Rh5AG355600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003968
Physical Location & Seq
Forward (+)
58967613 .. 58971510
3898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG355600.1

Sequence Viewer

Length: 1701 bp
ATGGTCATAGAGGAGTCAGCTGACGAGTACTGGAGATATGAATTTAGGGTTTATGGGACCCCTGAATTTCATAGTGAGGGTTTTACTCTAGAAATACACCATGGAGGGAGCTTTGTTGATGCAGGTGGTGGGCAACACAAATACATTGGAGGTGAAGTTCATTGGCTTGAAAAGCTTGATCCAGAAAAGACTTCATGGTTGGAGCTCAACACATTTGCTTGGAGACTTGGTTATAGGGAACCACCAGTTTCACTACTGATTCAAGCACCTATATTTGTCATGGTACATTCCCGTGTTAGGAGGGTAGAAGAAGCTGTGGCAGAGTTAGAGAAGGATTACTATGAGGAGATGATACCGCACATAATGTTCAAGCCAGAGATAATTATGGCTAGACTAAGGGCTGTTGACGTTGGTCAAAGTTCTTTTGGTCATACAAGTGCATTTGAATATCCAATTTCTCAATTGACTGAAGGTTCATTTGGTGGTCTAAGTAGTCAAACAATGGGGCATGGTGTTCTTTACCTTACTGATGAGCTAAATGGAGTAAAAGGTGTAGAACAAGACAAAACAGATAATGTAGGAGACTTGAGCAAGTTTGTGGATCATGTTGAAGAAGAATTTGAAGATGAAGACTCACAAGATGAGGACTATGTACCTCCAGACCCTTCAGAACCACCAGAGGAAGTGCTAGTTGATAGTGATTATGAAATCTTGCCAGAAGATGATAATGAGTTAGTACATGTGGTGGATGAAAGACAAGATGATGCAGACTTTAATAAACATGTTGACAATGAAGATGTCTTTGAAGAGTTCCATGACATGGGATATGCAGGTTACATCTCAGACCATTCTAATGATAGTGAAGAGTTGAGCGAGAAAGATGAATTAGATTCAGATTCAGATGAGGCAGCAAGTAACAAAAAGAAGAAGAAAAAGGTTAAGCTAGGTAGGGCATGGGTTCCTAAAAGGGATATGAAAGACCCAAAATTTGAGCTTGGCCAACATTTTGCCAACTCCAAACAGTTCAAGGAAGCTGTGAAGAAATACGCAGTGAAGAATGGGTTGAATTTATCTTTCAATAAGAACACAGCTAAGAAAGTTCAAGTGGTTTGTGGTAAAAAAGAAGAAGAGAGGTGGTCATTGTCTAGTCTGCTATGCCCACAAGCAATAACAGTAATCTGGGTTAAAGGTGATGACCCTATTAAATATGTGCATCATTATTATAGCAAGAAAGCATACCTTGATGCATATTCATATGTTATCAATCCAGTTCCTGGTATTGAGATGTGGAAGATCATTAAAGCTCCAGTCCAGTCTCCACCATATAGAAAACAACCTGGGGGACCCAAGATGGTGAGGCATAGAGAAGAAGGTGAGGAACCACCTGCCGCAAGTGAAAGAAGGCCAACCAAACTTCCTAGGACTAGATATGTGACTATGAGTTGTACAGTTTGTGGATCTGGGGATCACAACAGAAGAACCTGCCATATTGAACTTGCAAGGAAGGCCAGAGGAATTGGGGGACAAAGTAGTGGAGTTCCTGGAAATGTAAGGGTGAATGTGGATGTGAGTGTTGGAAAAAAGGGTGCTAACAATGTGAATGTACAAGTACAAACAGAAGCCGCACCAAAGGCCAAGAGAGCACCTAGGCATCAAGGGCACCTTCACCAAGAGTACCTAGGAGGGGAGGCCATACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

566

Amino Acids

64.13

Weight (kDa)

5.38

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 26 - 80 2.5e-10 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 113, 1393
Acc36I ACCTGC 4 cut(s) 113, 821, 1393, 1490
AccB1I GGYRCC 1 cut(s) 1659
AccB7I CCANNNNNTGG 2 cut(s) 820, 1274
AciI CCGC 3 cut(s) 356, 1389, 1623
AclWI GGATC 4 cut(s) 173, 609, 1465, 1473
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 5 cut(s) 41, 65, 617, 986, 1066
AcuI CTGAAG 2 cut(s) 489, 651
AfaI GTAC 8 cut(s) 29, 285, 654, 738, 1447, 1605, 1611, 1676
AfiI CCNNNNNNNGG 4 cut(s) 297, 820, 1274, 1684
AflIII ACRYGT 2 cut(s) 739, 781
AjnI CCWGG 3 cut(s) 1273, 1336, 1540
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
Alw21I GWGCWC 2 cut(s) 207, 1645
Alw26I GTCTC 3 cut(s) 217, 576, 1320
AlwI GGATC 4 cut(s) 173, 609, 1465, 1473
AlwNI CAGNNNCTG 1 cut(s) 1274
AoxI GGCC 5 cut(s) 997, 1403, 1506, 1632, 1689
ApeKI GCWGC 1 cut(s) 908
ApoI RAATTY 5 cut(s) 41, 65, 617, 986, 1066
ArsI GACNNNNNNTTYG 2 cut(s) 971, 1003
AspA2I CCTAGG 3 cut(s) 1418, 1646, 1678
AspS9I GGNCC 2 cut(s) 57, 1343
AsuHPI GGTGA 6 cut(s) 164, 1202, 1366, 1385, 1567, 1658
AvaII GGWCC 2 cut(s) 57, 1343
AvrII CCTAGG 3 cut(s) 1418, 1646, 1678
BaeGI GKGCMC 1 cut(s) 1662
BalI TGGCCA 1 cut(s) 999
BanI GGYRCC 1 cut(s) 1659
BanII GRGCYC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 2 cut(s) 207, 1645
BbvI GCAGC 1 cut(s) 920
BccI CCATC 1 cut(s) 1345
BciT130I CCWGG 3 cut(s) 1275, 1338, 1542
BcoDI GTCTC 3 cut(s) 217, 576, 1320
BfaI CTAG 9 cut(s) 89, 390, 689, 944, 1146, 1419, 1425, 1647, 1679
BfuAI ACCTGC 4 cut(s) 113, 821, 1393, 1490
BisI GCNGC 3 cut(s) 909, 1389, 1623
BlnI CCTAGG 3 cut(s) 1418, 1646, 1678
BlsI GCNGC 3 cut(s) 910, 1390, 1624
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 1275, 1338, 1542
Bme18I GGWCC 2 cut(s) 57, 1343
BmgT120I GGNCC 2 cut(s) 57, 1343
BmiI GGNNCC 8 cut(s) 58, 59, 240, 960, 1344, 1345, 1380, 1661
BmrFI CCNGG 3 cut(s) 1275, 1338, 1542
BmsI GCATC 5 cut(s) 109, 754, 1222, 1234, 1660
BoxI GACNNNNGTC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 636
BpmI CTGGAG 3 cut(s) 52, 642, 1290
BpuEI CTTGAG 1 cut(s) 607
BsaJI CCNNGG 5 cut(s) 100, 1337, 1418, 1646, 1678
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 214, 244
Bsc4I CCNNNNNNNGG 4 cut(s) 297, 820, 1274, 1684
Bse1I ACTGG 5 cut(s) 35, 245, 1268, 1307, 1312
BseBI CCWGG 3 cut(s) 1275, 1338, 1542
BseDI CCNNGG 5 cut(s) 100, 1337, 1418, 1646, 1678
BseGI GGATG 2 cut(s) 754, 1570
BseLI CCNNNNNNNGG 4 cut(s) 297, 820, 1274, 1684
BseMII CTCAG 1 cut(s) 855
BseNI ACTGG 5 cut(s) 35, 245, 1268, 1307, 1312
BseRI GAGGAG 2 cut(s) 26, 359
BseSI GKGCMC 1 cut(s) 1662
BseXI GCAGC 1 cut(s) 920
BshFI GGCC 5 cut(s) 999, 1405, 1508, 1634, 1691
BshNI GGYRCC 1 cut(s) 1659
BsiHKAI GWGCWC 2 cut(s) 207, 1645
BslFI GGGAC 3 cut(s) 70, 1356, 1536
BslI CCNNNNNNNGG 4 cut(s) 297, 820, 1274, 1684
BsmAI GTCTC 3 cut(s) 217, 576, 1320
BsmFI GGGAC 3 cut(s) 70, 1356, 1536
BsnI GGCC 5 cut(s) 999, 1405, 1508, 1634, 1691
Bsp1286I GDGCHC 3 cut(s) 207, 1645, 1662
Bsp1407I TGTACA 2 cut(s) 1445, 1603
Bsp143I GATC 5 cut(s) 178, 601, 1293, 1457, 1465
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 3 cut(s) 356, 1389, 1623
BspANI GGCC 5 cut(s) 999, 1405, 1508, 1634, 1691
BspCNI CTCAG 1 cut(s) 854
BspLI GGNNCC 8 cut(s) 58, 59, 240, 960, 1344, 1345, 1380, 1661
BspMI ACCTGC 4 cut(s) 113, 821, 1393, 1490
BspPI GGATC 4 cut(s) 173, 609, 1465, 1473
BspT107I GGYRCC 1 cut(s) 1659
BsrGI TGTACA 2 cut(s) 1445, 1603
BsrI ACTGG 5 cut(s) 35, 245, 1268, 1307, 1312
BssECI CCNNGG 5 cut(s) 100, 1337, 1418, 1646, 1678
BssMI GATC 5 cut(s) 178, 601, 1293, 1457, 1465
BssT1I CCWWGG 4 cut(s) 100, 1418, 1646, 1678
Bst2UI CCWGG 3 cut(s) 1275, 1338, 1542
Bst4CI ACNGT 3 cut(s) 1023, 1174, 1450
Bst6I CTCTTC 3 cut(s) 801, 858, 1122
BstAUI TGTACA 2 cut(s) 1445, 1603
BstDEI CTNAG 4 cut(s) 395, 488, 841, 1092
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 754, 1570
BstKTI GATC 5 cut(s) 181, 604, 1296, 1460, 1468
BstMAI GTCTC 3 cut(s) 217, 576, 1320
BstMBI GATC 5 cut(s) 178, 601, 1293, 1457, 1465
BstMWI GCNNNNNNNGC 5 cut(s) 172, 1505, 1631, 1640, 1657
BstNI CCWGG 3 cut(s) 1275, 1338, 1542
BstNSI RCATGY 2 cut(s) 743, 785
BstPAI GACNNNNGTC 1 cut(s) 411
BstSCI CCNGG 3 cut(s) 1273, 1336, 1540
BstSLI GKGCMC 1 cut(s) 1662
BstV1I GCAGC 1 cut(s) 920
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 1457
BstYI RGATCY 1 cut(s) 1457
BsuRI GGCC 5 cut(s) 999, 1405, 1508, 1634, 1691
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 754, 1570
BtsI GCAGTG 1 cut(s) 1056
BtsIMutI CAGTG 1 cut(s) 1056
BveI ACCTGC 4 cut(s) 113, 821, 1393, 1490
CaiI CAGNNNCTG 1 cut(s) 1274
Cfr13I GGNCC 2 cut(s) 57, 1343
Csp6I GTAC 8 cut(s) 28, 284, 653, 737, 1446, 1604, 1610, 1675
CviQI GTAC 8 cut(s) 28, 284, 653, 737, 1446, 1604, 1610, 1675
DdeI CTNAG 4 cut(s) 395, 488, 841, 1092
DpnI GATC 5 cut(s) 180, 603, 1295, 1459, 1467
DpnII GATC 5 cut(s) 178, 601, 1293, 1457, 1465
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 3 cut(s) 801, 858, 1122
EarI CTCTTC 3 cut(s) 801, 858, 1122
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 4 cut(s) 100, 1418, 1646, 1678
Eco24I GRGCYC 1 cut(s) 207
Eco47I GGWCC 2 cut(s) 57, 1343
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 489, 651
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 2 cut(s) 57, 1343
EcoRII CCWGG 3 cut(s) 1273, 1336, 1540
EcoT14I CCWWGG 4 cut(s) 100, 1418, 1646, 1678
EcoT22I ATGCAT 1 cut(s) 1249
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 4 cut(s) 100, 1418, 1646, 1678
FalI AAGNNNNNCTT 4 cut(s) 1224, 1256, 1647, 1679
FaqI GGGAC 3 cut(s) 70, 1356, 1536
FauNDI CATATG 1 cut(s) 1255
Fnu4HI GCNGC 3 cut(s) 909, 1389, 1623
FokI GGATG 2 cut(s) 761, 1577
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 3 cut(s) 909, 1389, 1623
FspBI CTAG 9 cut(s) 89, 390, 689, 944, 1146, 1419, 1425, 1647, 1679
GluI GCNGC 3 cut(s) 909, 1389, 1623
GsuI CTGGAG 3 cut(s) 52, 642, 1290
HaeIII GGCC 5 cut(s) 999, 1405, 1508, 1634, 1691
HincII GTYRAC 2 cut(s) 406, 787
HindII GTYRAC 2 cut(s) 406, 787
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 5 cut(s) 14, 259, 632, 890, 896
HphI GGTGA 6 cut(s) 164, 1202, 1366, 1385, 1567, 1658
Hpy166II GTNNAC 2 cut(s) 406, 787
Hpy188I TCNGA 4 cut(s) 670, 844, 895, 901
Hpy188III TCNNGA 3 cut(s) 89, 182, 659
Hpy8I GTNNAC 2 cut(s) 406, 787
HpyAV CCTTC 7 cut(s) 325, 464, 675, 1364, 1395, 1498, 1673
HpyCH4III ACNGT 3 cut(s) 1023, 1174, 1450
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 7 cut(s) 122, 440, 767, 830, 1213, 1247, 1499
HpyF10VI GCNNNNNNNGC 5 cut(s) 172, 1505, 1631, 1640, 1657
HpyF3I CTNAG 4 cut(s) 395, 488, 841, 1092
HpySE526I ACGT 1 cut(s) 408
KflI GGGWCCC 2 cut(s) 57, 1343
Kzo9I GATC 5 cut(s) 178, 601, 1293, 1457, 1465
LmnI GCTCC 3 cut(s) 108, 202, 1309
Lsp1109I GCAGC 1 cut(s) 920
LweI GCATC 5 cut(s) 109, 754, 1222, 1234, 1660
MaeI CTAG 9 cut(s) 89, 390, 689, 944, 1146, 1419, 1425, 1647, 1679
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 3 cut(s) 833, 914, 1432
MalI GATC 5 cut(s) 180, 603, 1295, 1459, 1467
MboI GATC 5 cut(s) 178, 601, 1293, 1457, 1465
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 1 cut(s) 1457
MhlI GDGCHC 3 cut(s) 207, 1645, 1662
MlsI TGGCCA 1 cut(s) 999
MluNI TGGCCA 1 cut(s) 999
MlyI GAGTC 2 cut(s) 23, 626
MmeI TCCRAC 2 cut(s) 180, 1555
Mox20I TGGCCA 1 cut(s) 999
Mph1103I ATGCAT 1 cut(s) 1249
MscI TGGCCA 1 cut(s) 999
MseI TTAA 5 cut(s) 774, 939, 1185, 1203, 1299
MslI CAYNNNNRTG 3 cut(s) 291, 435, 852
Msp20I TGGCCA 1 cut(s) 999
MspA1I CMGCKG 1 cut(s) 20
MspR9I CCNGG 3 cut(s) 1275, 1338, 1542
MunI CAATTG 1 cut(s) 461
MvaI CCWGG 3 cut(s) 1275, 1338, 1542
MwoI GCNNNNNNNGC 5 cut(s) 172, 1505, 1631, 1640, 1657
NcoI CCATGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 1255
NdeII GATC 5 cut(s) 178, 601, 1293, 1457, 1465
NlaIV GGNNCC 8 cut(s) 58, 59, 240, 960, 1344, 1345, 1380, 1661
NmuCI GTSAC 1 cut(s) 1432
NsiI ATGCAT 1 cut(s) 1249
NspI RCATGY 2 cut(s) 743, 785
PaqCI CACCTGC 2 cut(s) 113, 1393
PciI ACATGT 2 cut(s) 739, 781
PfeI GAWTC 3 cut(s) 259, 890, 896
PflMI CCANNNNNTGG 2 cut(s) 820, 1274
PfoI TCCNGGA 1 cut(s) 1540
PkrI GCNGC 3 cut(s) 910, 1390, 1624
PleI GAGTC 2 cut(s) 22, 626
PpsI GAGTC 2 cut(s) 22, 626
PpuMI RGGWCCY 2 cut(s) 57, 1343
PscI ACATGT 2 cut(s) 739, 781
PshAI GACNNNNGTC 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 207
Psp5II RGGWCCY 2 cut(s) 57, 1343
Psp6I CCWGG 3 cut(s) 1273, 1336, 1540
PspGI CCWGG 3 cut(s) 1273, 1336, 1540
PspN4I GGNNCC 8 cut(s) 58, 59, 240, 960, 1344, 1345, 1380, 1661
PspPI GGNCC 2 cut(s) 57, 1343
PspPPI RGGWCCY 2 cut(s) 57, 1343
PstNI CAGNNNCTG 1 cut(s) 1274
PsuI RGATCY 1 cut(s) 1457
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 8 cut(s) 29, 285, 654, 738, 1447, 1605, 1611, 1676
RsaNI GTAC 8 cut(s) 28, 284, 653, 737, 1446, 1604, 1610, 1675
RseI CAYNNNNRTG 3 cut(s) 291, 435, 852
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 5 cut(s) 774, 939, 1185, 1203, 1299
SatI GCNGC 3 cut(s) 909, 1389, 1623
Sau3AI GATC 5 cut(s) 178, 601, 1293, 1457, 1465
Sau96I GGNCC 2 cut(s) 57, 1343
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 2 cut(s) 23, 626
ScrFI CCNGG 3 cut(s) 1275, 1338, 1542
SduI GDGCHC 3 cut(s) 207, 1645, 1662
SfaNI GCATC 5 cut(s) 109, 754, 1222, 1234, 1660
SinI GGWCC 2 cut(s) 57, 1343
SmiMI CAYNNNNRTG 3 cut(s) 291, 435, 852
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
SsiI CCGC 3 cut(s) 356, 1389, 1623
SspMI CTAG 9 cut(s) 89, 390, 689, 944, 1146, 1419, 1425, 1647, 1679
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 3 cut(s) 1273, 1336, 1540
StyI CCWWGG 4 cut(s) 100, 1418, 1646, 1678
TaaI ACNGT 3 cut(s) 1023, 1174, 1450
TaiI ACGT 1 cut(s) 411
TatI WGTACW 5 cut(s) 27, 736, 1445, 1603, 1609
TauI GCSGC 2 cut(s) 1391, 1625
TfiI GAWTC 3 cut(s) 259, 890, 896
Tru1I TTAA 5 cut(s) 774, 939, 1185, 1203, 1299
Tru9I TTAA 5 cut(s) 774, 939, 1185, 1203, 1299
TscAI CASTG 1 cut(s) 1056
TseFI GTSAC 1 cut(s) 1432
TseI GCWGC 1 cut(s) 908
Tsp45I GTSAC 1 cut(s) 1432
TspRI CASTG 1 cut(s) 1056
Van91I CCANNNNNTGG 2 cut(s) 820, 1274
VpaK11BI GGWCC 2 cut(s) 57, 1343
XapI RAATTY 5 cut(s) 41, 65, 617, 986, 1066
XbaI TCTAGA 1 cut(s) 88
XceI RCATGY 2 cut(s) 743, 785
XmaJI CCTAGG 3 cut(s) 1418, 1646, 1678
XspI CTAG 9 cut(s) 89, 390, 689, 944, 1146, 1419, 1425, 1647, 1679
ZrmI AGTACT 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.