Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003934

Physical Location & Seq

Genomic Coordinates Forward (+)

29542008 .. 29544485

Gene Structure 2478 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018419

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGGCTTCTTCTTCTGTACTGGTTATATTCTTACTCAGTACTGTTTTGATTATCTCGATCATGCCCGATGTAAAAGCTCAAAGAAACCACTCCAAATCCAATTTAATCAGCCTGGGCTCTTCTCTATCCCCCCATGCTAATAACCTCAATATTTCTTCATGGCTGTCATCTTCTGGCCGTTTCGCCTTCGGTTTCTACCCACAAGGCAACGGCTTTGCTGTTGGTATGCAGCTTCTTAATCCACCACAAAACACAGTTGTGTGGACTGCAAATCGAGATGACCCCCCTGTCTCCCCAAACTCTACACTGGAACTAACAAGAGACGGTCTCTTGCTTCGGTCAGAGGGAGGCCAAGAAATACGTATTACTAATGGCTCAAGTTCAGTAGAAACTAGTGCGATATCAAATGCATCAACTGCAGCTGCAGCTATGCTTGATTCCGGAAACTTTGTGCTGTATAATGAGACTTCTTTTGTTATATGGGAGAGCTTTGATTTCCCAACTGATACAATACTGGGAGGCCAAGATTTGCGGATGTCCATGTATCTGGTATCTAGTGCATCTGTTGCAGACCACTCCAGCGGGCGATTTTCCCTGAAGATGCAGGTTGATGGAAACCTTGTGGCCTACCCGCCAAACAGTTCTTTTGCTTATTGGGCTACTATGACCTCCGGTAGTCACCAACTTAGTCTCAACCTATCAGGTTTTCTCGTTTTGAATAGTGGTCTGACTACTCAGGTCTTGGCAAACGGCTCGTACCATCACCCACAAGACAATCAAACCACAATCTACCGAGCAACGCTTGATGTCGACGGGAATTTCAGATTGTACTTGCATAACTTTCTGATGTCCGGTAGTTCAGAGGGAGCTGTAATGCCTATTTGGTCAGCTTTGCAACATCCATGCGACGTCCATGGCATTTGTGGCTTCAACAGTTATTGTGAAGTAGTCATGCAAAAGAAAGCAGCTGAAGTTCAGTGCAAATGTTACCCTGGATTTGTTTACAACAACGCCAGTCAGAAGTCTCTGGGGTGCTATCATAATTTCACTGTAGACGGCTGCACCGTAAATGAAGAGCCACGGCTGCGGTACAAGGTAGCGGCCTTGGACAATACATCGTGGAGTAATCATCCTTATTCAGTGACACCTATTCGACACAAGGAAGATTGCAATGACTCTTGCCTGAGAGACTGCAATTGTTGGGCTGTGCTACATACAGATGCTGATTGCAAAAAATACAAGCTTCCACTTTTATATGGCAAAACAACTTCAAGTATATCCTCTAAGGGTTACTTCAAAGTGCTTCTACCGAGAAATATGGATGGCATGCCAAACAATTTTCCCGAACTAACCGAAAGCCAGACGGTAACTGAAAGCAAGAATAACAGTCTAATTCTAATTCTTGCCATCACTCTGGGTTCCATTGCACTCTTGTGTTTAGTCCTTGCCATCTCAAGTTTCACCTTATACAAGCACCGAGTTCATAGGTACGAGAAGCTTTTGAAACATTCGAGTTTGGCATTAGCTGATCAAGAGTACTTCACTTTACAATCGTTTTCTTATGCTGAGCTCGAAATAGCAACAGATGGCTTCAAGGAAGAGATAGGAAGGGGTACTTTCGGAGCTGTTTATAAAGGAACTCTGTCAGGGGCCAGCTCTAACAAAACTGTTGCTGTTAAAAGACTGGAGAAAGTGGTTGATGAAGGAGTAAGGGAATTTATAGCTGAAATTACCACCATTGGACGAACTCACCACAGAAACTTAGTTCAGTTGCTTGGTTTCTGCATTGAGGGTTCTCGGAAGCTTCTTGTTTATGAATATATGAGCAACGGCTCACTTGCAGATCTTATCTTTAAGGCCAAAAGTGTGCACTCATCTTGGAAAGAAAGGGTAAGACTTGTATTCGATGTGGCTAAAGGGGTCCTCTATTTACATGACGAATGCAGTGTCCATATCATTCACTCCAATTTGAAACCCCAAAACGTACTCTTGGATCATACTTGGACTGCTAAGATATCTGATTTCGGTTTGGCCAGGCTGGTGCCAAATGAAACAAAAATCAACTCTACTGGAGGAGGAGGCGCGGGAACAGTCGAACAGGTGAGAAGAGGTTACTTGGCGCCTGAATGGGAGAAGAATGCTCTGATATCTGTAAAAGCTGATATCTACAGTTTTGGTATTGTGCTTCTAGAGATTGTATGTTGTAGAAGAAACATAGAAGTAAATGTTTCAACACCAGATGAGATAATTCTTTCGGGTTGGGTCAGTAAATGCTTTGCTGCTGGAGAATTAGATAAGCTTGTGGAGGACGATGAAAACGTAGATTTGAAGACCCTAGAAAGAATGGTGAAGGCGGGGTTGTGGTGTGTTCAACATGATCCAGCTTTGCGTCCTTTGATGAAGAATGTAATCTTGATGTTGGAAGGGACAATGGACATACCAGTTCCACCATGTCCAGAGCTTCCCTCTCTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

826

Amino Acids

90.91

Weight (kDa)

6.26

Isoelectric Point (pI)

35.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	85 - 180	1e-16	D-mannose binding lectin
S_locus_glycop	PF00954	240 - 335	9.1e-06	S-locus glycoprotein domain
PK_Tyr_Ser-Thr	PF07714	531 - 751	8.3e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	532 - 753	1e-39	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003934)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23390
prunus_persica	Prupe.6G165100_v2.0.a1 Prupe.6G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0118681 RchiOBHm_Chr2g0118741 RchiOBHm_Chr2g0118761
rosa_laevigata	RLG00000018419
rosa_multiflora	Rmu_co8322353.1_g000001 Rmu_sc0001814.1_g000026 Rmu_sc0001814.1_g000032 Rmu_sc0002700.1_g000009 Rmu_sc0002700.1_g000033 Rmu_sc0002700.1_g000035 Rmu_sc0002831.1_g000014 Rmu_sc0002831.1_g000017 Rmu_sc0002831.1_g000031 Rmu_sc0004399.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124760 Rroxscaffold_2G00124850 Rroxscaffold_2G00124890 Rroxscaffold_2G00125410 Rroxscaffold_2G00125420
rosa_rugosa	Rorug02G0218400
rosa_samantha	Rh2AG274700 Rh2AG275000 Rh2AG275300 Rh2BG286500 Rh2BG286700 Rh2BG287100 Rh2DG301500 Rh2DG301700
rosa_wichuraiana	Rw2G021850 Rw2G021890 Rw2G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1632
AasI	GACNNNNNNGTC	1 cut(s)	287
AatII	GACGTC	1 cut(s)	912
Acc36I	ACCTGC	1 cut(s)	595
AccB1I	GGYRCC	2 cut(s)	2041, 2119
AccI	GTMKAC	2 cut(s)	810, 1053
AccII	CGCG	1 cut(s)	2084
AccIII	TCCGGA	1 cut(s)	440
AciI	CCGC	7 cut(s)	532, 582, 632, 1087, 1100, 2084, 2354
AclWI	GGATC	2 cut(s)	2001, 2372
AcoI	YGGCCR	2 cut(s)	175, 2031
AcsI	RAATTY	2 cut(s)	817, 1715
AcuI	CTGAAG	2 cut(s)	617, 990
AcyI	GRCGYC	2 cut(s)	909, 2120
AfaI	GTAC	9 cut(s)	18, 40, 758, 830, 1091, 1490, 1538, 1615, 1986
AhlI	ACTAGT	1 cut(s)	392
AjnI	CCWGG	3 cut(s)	111, 991, 2033
AleI	CACNNNNGTG	2 cut(s)	257, 1432
Alw21I	GWGCWC	2 cut(s)	1572, 1872
Alw26I	GTCTC	7 cut(s)	295, 315, 332, 458, 695, 1029, 1182
Alw44I	GTGCAC	1 cut(s)	1868
AlwI	GGATC	2 cut(s)	2001, 2372
AlwNI	CAGNNNCTG	1 cut(s)	1223
Aor13HI	TCCGGA	1 cut(s)	440
AoxI	GGCC	8 cut(s)	175, 349, 520, 624, 1101, 1650, 1857, 2031
ApaLI	GTGCAC	1 cut(s)	1868
ApeKI	GCWGC	8 cut(s)	229, 419, 422, 425, 965, 1059, 1084, 2279
ApoI	RAATTY	2 cut(s)	817, 1715
ArsI	GACNNNNNNTTYG	2 cut(s)	1932, 1964
AspLEI	GCGC	2 cut(s)	2084, 2122
AspS9I	GGNCC	2 cut(s)	1650, 1921
AsuHPI	GGTGA	6 cut(s)	671, 755, 1453, 1742, 2113, 2359
AvaII	GGWCC	1 cut(s)	1921
BaeGI	GKGCMC	1 cut(s)	1872
BalI	TGGCCA	1 cut(s)	2033
BanI	GGYRCC	2 cut(s)	2041, 2119
BanII	GRGCYC	2 cut(s)	119, 1572
BbsI	GAAGAC	1 cut(s)	2336
Bbv12I	GWGCWC	2 cut(s)	1572, 1872
BbvI	GCAGC	8 cut(s)	241, 409, 431, 437, 977, 1046, 1071, 2266
BccI	CCATC	6 cut(s)	605, 768, 1316, 1415, 1457, 1580
BceAI	ACGGC	6 cut(s)	162, 226, 766, 1072, 1097, 1846
BcgI	CGANNNNNNTGC	2 cut(s)	735, 769
BciT130I	CCWGG	3 cut(s)	113, 993, 2035
BclI	TGATCA	1 cut(s)	1528
BcoDI	GTCTC	7 cut(s)	295, 315, 332, 458, 695, 1029, 1182
BcuI	ACTAGT	1 cut(s)	392
BfaI	CTAG	4 cut(s)	393, 555, 2189, 2336
BfmI	CTRYAG	4 cut(s)	417, 423, 1050, 2167
BfoI	RGCGCY	1 cut(s)	2123
BfuAI	ACCTGC	1 cut(s)	595
BglII	AGATCT	1 cut(s)	1843
BisI	GCNGC	9 cut(s)	230, 420, 423, 426, 966, 1060, 1085, 1101, 2280
BlpI	GCTNAGC	1 cut(s)	1566
BlsI	GCNGC	9 cut(s)	231, 421, 424, 427, 967, 1061, 1086, 1102, 2281
BmcAI	AGTACT	2 cut(s)	40, 1538
Bme1390I	CCNGG	3 cut(s)	113, 993, 2035
Bme18I	GGWCC	1 cut(s)	1921
BmgT120I	GGNCC	2 cut(s)	1650, 1921
BmiI	GGNNCC	5 cut(s)	1420, 1651, 1922, 2043, 2121
BmrFI	CCNGG	3 cut(s)	113, 993, 2035
BmrI	ACTGGG	1 cut(s)	524
BmsI	GCATC	4 cut(s)	419, 569, 591, 1210
BmuI	ACTGGG	1 cut(s)	524
BpiI	GAAGAC	1 cut(s)	2336
BplI	GAGNNNNNCTC	3 cut(s)	312, 344, 2450
BpmI	CTGGAG	4 cut(s)	562, 1706, 2091, 2304
Bpu1102I	GCTNAGC	1 cut(s)	1566
BpuEI	CTTGAG	2 cut(s)	361, 1438
BsaAI	YACGTR	1 cut(s)	362
BsaHI	GRCGYC	2 cut(s)	909, 2120
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	5 cut(s)	112, 913, 991, 1079, 1104
BsaWI	WCCGGW	3 cut(s)	440, 671, 851
Bse1I	ACTGG	7 cut(s)	24, 312, 519, 1014, 1689, 2074, 2441
Bse3DI	GCAATG	2 cut(s)	1177, 1422
BseAI	TCCGGA	1 cut(s)	440
BseBI	CCWGG	3 cut(s)	113, 993, 2035
BseDI	CCNNGG	5 cut(s)	112, 913, 991, 1079, 1104
BseGI	GGATG	4 cut(s)	540, 898, 1129, 1327
BseMI	GCAATG	2 cut(s)	1177, 1422
BseMII	CTCAG	4 cut(s)	49, 749, 1175, 1557
BseNI	ACTGG	7 cut(s)	24, 312, 519, 1014, 1689, 2074, 2441
BseRI	GAGGAG	2 cut(s)	2088, 2091
BseSI	GKGCMC	1 cut(s)	1872
BseXI	GCAGC	8 cut(s)	241, 409, 431, 437, 977, 1046, 1071, 2266
BsgI	GTGCAG	1 cut(s)	1045
Bsh1236I	CGCG	1 cut(s)	2084
BshFI	GGCC	8 cut(s)	177, 351, 522, 626, 1103, 1652, 1859, 2033
BshNI	GGYRCC	2 cut(s)	2041, 2119
BsiHKAI	GWGCWC	2 cut(s)	1572, 1872
BsiSI	CCGG	3 cut(s)	441, 672, 852
BslFI	GGGAC	1 cut(s)	2440
BsmAI	GTCTC	7 cut(s)	295, 315, 332, 458, 695, 1029, 1182
BsmBI	CGTCTC	1 cut(s)	315
BsmFI	GGGAC	1 cut(s)	2440
BsmI	GAATGC	2 cut(s)	1946, 2143
BsnI	GGCC	8 cut(s)	177, 351, 522, 626, 1103, 1652, 1859, 2033
Bso31I	GGTCTC	1 cut(s)	332
Bsp1286I	GDGCHC	3 cut(s)	119, 1572, 1872
Bsp13I	TCCGGA	1 cut(s)	440
Bsp143I	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
Bsp1720I	GCTNAGC	1 cut(s)	1566
Bsp19I	CCATGG	1 cut(s)	913
BspACI	CCGC	7 cut(s)	532, 582, 632, 1087, 1100, 2084, 2354
BspANI	GGCC	8 cut(s)	177, 351, 522, 626, 1103, 1652, 1859, 2033
BspCNI	CTCAG	4 cut(s)	48, 748, 1176, 1558
BspEI	TCCGGA	1 cut(s)	440
BspFNI	CGCG	1 cut(s)	2084
BspLI	GGNNCC	5 cut(s)	1420, 1651, 1922, 2043, 2121
BspMAI	CTGCAG	2 cut(s)	421, 427
BspMI	ACCTGC	1 cut(s)	595
BspPI	GGATC	2 cut(s)	2001, 2372
BspQI	GCTCTTC	2 cut(s)	124, 1068
BspT107I	GGYRCC	2 cut(s)	2041, 2119
BspTNI	GGTCTC	1 cut(s)	332
BsrDI	GCAATG	2 cut(s)	1177, 1422
BsrI	ACTGG	7 cut(s)	24, 312, 519, 1014, 1689, 2074, 2441
BssECI	CCNNGG	5 cut(s)	112, 913, 991, 1079, 1104
BssMI	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
BssNI	GRCGYC	2 cut(s)	909, 2120
BssT1I	CCWWGG	2 cut(s)	913, 1104
Bst2UI	CCWGG	3 cut(s)	113, 993, 2035
Bst6I	CTCTTC	4 cut(s)	124, 1068, 1593, 2101
BstACI	GRCGYC	2 cut(s)	909, 2120
BstAPI	GCANNNNNTGC	2 cut(s)	416, 566
BstBAI	YACGTR	1 cut(s)	362
BstC8I	GCNNGC	3 cut(s)	584, 1328, 1654
BstDEI	CTNAG	8 cut(s)	35, 686, 735, 1184, 1284, 1566, 1762, 2010
BstDSI	CCRYGG	2 cut(s)	913, 1079
BstF5I	GGATG	4 cut(s)	540, 898, 1129, 1327
BstFNI	CGCG	1 cut(s)	2084
BstH2I	RGCGCY	1 cut(s)	2123
BstHHI	GCGC	2 cut(s)	2084, 2122
BstKTI	GATC	5 cut(s)	60, 1531, 1846, 1996, 2380
BstMAI	GTCTC	7 cut(s)	295, 315, 332, 458, 695, 1029, 1182
BstMBI	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
BstMWI	GCNNNNNNNGC	6 cut(s)	416, 425, 566, 656, 924, 1084
BstNI	CCWGG	3 cut(s)	113, 993, 2035
BstNSI	RCATGY	1 cut(s)	1330
BstSCI	CCNGG	3 cut(s)	111, 991, 2033
BstSFI	CTRYAG	4 cut(s)	417, 423, 1050, 2167
BstSLI	GKGCMC	1 cut(s)	1872
BstSNI	TACGTA	1 cut(s)	362
BstUI	CGCG	1 cut(s)	2084
BstV1I	GCAGC	8 cut(s)	241, 409, 431, 437, 977, 1046, 1071, 2266
BstV2I	GAAGAC	1 cut(s)	2336
BstX2I	RGATCY	1 cut(s)	1843
BstXI	CCANNNNNNTGG	2 cut(s)	547, 1414
BstYI	RGATCY	1 cut(s)	1843
BsuRI	GGCC	8 cut(s)	177, 351, 522, 626, 1103, 1652, 1859, 2033
BtgI	CCRYGG	2 cut(s)	913, 1079
BtsCI	GGATG	4 cut(s)	540, 898, 1129, 1327
BtsI	GCAGTG	1 cut(s)	1951
BtsIMutI	CAGTG	5 cut(s)	305, 983, 1047, 1146, 1951
BveI	ACCTGC	1 cut(s)	595
Cac8I	GCNNGC	3 cut(s)	584, 1328, 1654
CaiI	CAGNNNCTG	1 cut(s)	1223
CfoI	GCGC	2 cut(s)	2084, 2122
Cfr13I	GGNCC	2 cut(s)	1650, 1921
CseI	GACGC	1 cut(s)	2378
Csp6I	GTAC	9 cut(s)	17, 39, 757, 829, 1090, 1489, 1537, 1614, 1985
CviQI	GTAC	9 cut(s)	17, 39, 757, 829, 1090, 1489, 1537, 1614, 1985
DdeI	CTNAG	8 cut(s)	35, 686, 735, 1184, 1284, 1566, 1762, 2010
DinI	GGCGCC	1 cut(s)	2121
DpnI	GATC	5 cut(s)	59, 1530, 1845, 1995, 2379
DpnII	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
DrdI	GACNNNNNNGTC	1 cut(s)	287
DseDI	GACNNNNNNGTC	1 cut(s)	287
EaeI	YGGCCR	2 cut(s)	175, 2031
Eam1104I	CTCTTC	4 cut(s)	124, 1068, 1593, 2101
EarI	CTCTTC	4 cut(s)	124, 1068, 1593, 2101
Ecl136II	GAGCTC	1 cut(s)	1570
Eco105I	TACGTA	1 cut(s)	362
Eco130I	CCWWGG	2 cut(s)	913, 1104
Eco24I	GRGCYC	2 cut(s)	119, 1572
Eco31I	GGTCTC	1 cut(s)	332
Eco32I	GATATC	4 cut(s)	402, 2016, 2148, 2164
Eco47I	GGWCC	1 cut(s)	1921
Eco53kI	GAGCTC	1 cut(s)	1570
Eco57I	CTGAAG	2 cut(s)	617, 990
EcoICRI	GAGCTC	1 cut(s)	1570
EcoO109I	RGGNCCY	1 cut(s)	1921
EcoRII	CCWGG	3 cut(s)	111, 991, 2033
EcoRV	GATATC	4 cut(s)	402, 2016, 2148, 2164
EcoT14I	CCWWGG	2 cut(s)	913, 1104
EcoT22I	ATGCAT	1 cut(s)	412
EcoT38I	GRGCYC	2 cut(s)	119, 1572
EgeI	GGCGCC	1 cut(s)	2121
EheI	GGCGCC	1 cut(s)	2121
ErhI	CCWWGG	2 cut(s)	913, 1104
Esp3I	CGTCTC	1 cut(s)	315
FalI	AAGNNNNNCTT	4 cut(s)	1277, 1309, 1600, 1632
FaqI	GGGAC	1 cut(s)	2440
FauI	CCCGC	4 cut(s)	575, 639, 2077, 2347
FbaI	TGATCA	1 cut(s)	1528
FblI	GTMKAC	2 cut(s)	810, 1053
Fnu4HI	GCNGC	9 cut(s)	230, 420, 423, 426, 966, 1060, 1085, 1101, 2280
FokI	GGATG	4 cut(s)	547, 885, 1116, 1334
FriOI	GRGCYC	2 cut(s)	119, 1572
Fsp4HI	GCNGC	9 cut(s)	230, 420, 423, 426, 966, 1060, 1085, 1101, 2280
FspBI	CTAG	4 cut(s)	393, 555, 2189, 2336
GlaI	GCGC	2 cut(s)	2083, 2121
GluI	GCNGC	9 cut(s)	230, 420, 423, 426, 966, 1060, 1085, 1101, 2280
GsuI	CTGGAG	4 cut(s)	562, 1706, 2091, 2304
HaeII	RGCGCY	1 cut(s)	2123
HaeIII	GGCC	8 cut(s)	177, 351, 522, 626, 1103, 1652, 1859, 2033
HapII	CCGG	3 cut(s)	441, 672, 852
HgaI	GACGC	1 cut(s)	2378
HhaI	GCGC	2 cut(s)	2084, 2122
Hin1I	GRCGYC	2 cut(s)	909, 2120
Hin6I	GCGC	2 cut(s)	2082, 2120
HinP1I	GCGC	2 cut(s)	2082, 2120
HincII	GTYRAC	1 cut(s)	811
HindII	GTYRAC	1 cut(s)	811
HindIII	AAGCTT	4 cut(s)	1241, 1496, 1802, 2297
HinfI	GANTC	2 cut(s)	437, 1175
HpaII	CCGG	3 cut(s)	441, 672, 852
HphI	GGTGA	6 cut(s)	671, 755, 1453, 1742, 2113, 2359
Hpy166II	GTNNAC	5 cut(s)	264, 811, 1003, 1054, 1870
Hpy188III	TCNNGA	8 cut(s)	55, 275, 441, 1343, 1532, 2189, 2413, 2456
Hpy8I	GTNNAC	5 cut(s)	264, 811, 1003, 1054, 1870
Hpy99I	CGWCG	2 cut(s)	815, 911
HpyAV	CCTTC	5 cut(s)	196, 1602, 1697, 2344, 2417
HpyCH4IV	ACGT	4 cut(s)	361, 909, 1983, 2319
HpyF10VI	GCNNNNNNNGC	6 cut(s)	416, 425, 566, 656, 924, 1084
HpyF3I	CTNAG	8 cut(s)	35, 686, 735, 1184, 1284, 1566, 1762, 2010
HpySE526I	ACGT	4 cut(s)	361, 909, 1983, 2319
Hsp92I	GRCGYC	2 cut(s)	909, 2120
HspAI	GCGC	2 cut(s)	2082, 2120
KasI	GGCGCC	1 cut(s)	2119
Kpn2I	TCCGGA	1 cut(s)	440
Ksp22I	TGATCA	1 cut(s)	1528
Kzo9I	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
LguI	GCTCTTC	2 cut(s)	124, 1068
LmnI	GCTCC	2 cut(s)	866, 1622
Lsp1109I	GCAGC	8 cut(s)	241, 409, 431, 437, 977, 1046, 1071, 2266
LweI	GCATC	4 cut(s)	419, 569, 591, 1210
MaeI	CTAG	4 cut(s)	393, 555, 2189, 2336
MaeII	ACGT	4 cut(s)	361, 909, 1983, 2319
MaeIII	GTNAC	6 cut(s)	677, 986, 1141, 1289, 1366, 2111
MalI	GATC	5 cut(s)	59, 1530, 1845, 1995, 2379
MboI	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
MfeI	CAATTG	1 cut(s)	1195
MflI	RGATCY	1 cut(s)	1843
MhlI	GDGCHC	3 cut(s)	119, 1572, 1872
MlsI	TGGCCA	1 cut(s)	2033
MluNI	TGGCCA	1 cut(s)	2033
Mly113I	GGCGCC	1 cut(s)	2120
MlyI	GAGTC	1 cut(s)	1169
MmeI	TCCRAC	1 cut(s)	2400
Mox20I	TGGCCA	1 cut(s)	2033
Mph1103I	ATGCAT	1 cut(s)	412
MroI	TCCGGA	1 cut(s)	440
MscI	TGGCCA	1 cut(s)	2033
MseI	TTAA	4 cut(s)	104, 237, 1677, 1854
MslI	CAYNNNNRTG	3 cut(s)	257, 1218, 1432
Msp20I	TGGCCA	1 cut(s)	2033
MspA1I	CMGCKG	3 cut(s)	422, 582, 968
MspI	CCGG	3 cut(s)	441, 672, 852
MspR9I	CCNGG	3 cut(s)	113, 993, 2035
MunI	CAATTG	1 cut(s)	1195
Mva1269I	GAATGC	2 cut(s)	1946, 2143
MvaI	CCWGG	3 cut(s)	113, 993, 2035
MvnI	CGCG	1 cut(s)	2084
MwoI	GCNNNNNNNGC	6 cut(s)	416, 425, 566, 656, 924, 1084
NarI	GGCGCC	1 cut(s)	2120
NcoI	CCATGG	1 cut(s)	913
NdeII	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
NlaIV	GGNNCC	5 cut(s)	1420, 1651, 1922, 2043, 2121
NmuCI	GTSAC	2 cut(s)	677, 1141
NsiI	ATGCAT	1 cut(s)	412
NspI	RCATGY	1 cut(s)	1330
OliI	CACNNNNGTG	2 cut(s)	257, 1432
PaeI	GCATGC	1 cut(s)	1330
PciSI	GCTCTTC	2 cut(s)	124, 1068
PcsI	WCGNNNNNNNCGW	2 cut(s)	1062, 2316
PctI	GAATGC	2 cut(s)	1946, 2143
PfeI	GAWTC	1 cut(s)	437
PkrI	GCNGC	9 cut(s)	231, 421, 424, 427, 967, 1061, 1086, 1102, 2281
PleI	GAGTC	1 cut(s)	1169
PluTI	GGCGCC	1 cut(s)	2123
PpsI	GAGTC	1 cut(s)	1169
Ppu21I	YACGTR	1 cut(s)	362
PpuMI	RGGWCCY	1 cut(s)	1921
PsiI	TTATAA	1 cut(s)	1632
Psp124BI	GAGCTC	1 cut(s)	1572
Psp5II	RGGWCCY	1 cut(s)	1921
Psp6I	CCWGG	3 cut(s)	111, 991, 2033
PspGI	CCWGG	3 cut(s)	111, 991, 2033
PspN4I	GGNNCC	5 cut(s)	1420, 1651, 1922, 2043, 2121
PspPI	GGNCC	2 cut(s)	1650, 1921
PspPPI	RGGWCCY	1 cut(s)	1921
PstI	CTGCAG	2 cut(s)	421, 427
PstNI	CAGNNNCTG	1 cut(s)	1223
PsuI	RGATCY	1 cut(s)	1843
PvuII	CAGCTG	2 cut(s)	422, 968
RsaI	GTAC	9 cut(s)	18, 40, 758, 830, 1091, 1490, 1538, 1615, 1986
RsaNI	GTAC	9 cut(s)	17, 39, 757, 829, 1090, 1489, 1537, 1614, 1985
RseI	CAYNNNNRTG	3 cut(s)	257, 1218, 1432
SacI	GAGCTC	1 cut(s)	1572
SalI	GTCGAC	1 cut(s)	809
SapI	GCTCTTC	2 cut(s)	124, 1068
SaqAI	TTAA	4 cut(s)	104, 237, 1677, 1854
SatI	GCNGC	9 cut(s)	230, 420, 423, 426, 966, 1060, 1085, 1101, 2280
Sau3AI	GATC	5 cut(s)	57, 1528, 1843, 1993, 2377
Sau96I	GGNCC	2 cut(s)	1650, 1921
ScaI	AGTACT	2 cut(s)	40, 1538
SchI	GAGTC	1 cut(s)	1169
ScrFI	CCNGG	3 cut(s)	113, 993, 2035
SduI	GDGCHC	3 cut(s)	119, 1572, 1872
SfaNI	GCATC	4 cut(s)	419, 569, 591, 1210
SfcI	CTRYAG	4 cut(s)	417, 423, 1050, 2167
SfoI	GGCGCC	1 cut(s)	2121
SinI	GGWCC	1 cut(s)	1921
SmiMI	CAYNNNNRTG	3 cut(s)	257, 1218, 1432
SmlI	CTYRAG	2 cut(s)	376, 1453
SmoI	CTYRAG	2 cut(s)	376, 1453
SnaBI	TACGTA	1 cut(s)	362
SpeI	ACTAGT	1 cut(s)	392
SphI	GCATGC	1 cut(s)	1330
SsiI	CCGC	7 cut(s)	532, 582, 632, 1087, 1100, 2084, 2354
SspDI	GGCGCC	1 cut(s)	2119
SspI	AATATT	1 cut(s)	151
SspMI	CTAG	4 cut(s)	393, 555, 2189, 2336
SstI	GAGCTC	1 cut(s)	1572
StyD4I	CCNGG	3 cut(s)	111, 991, 2033
StyI	CCWWGG	2 cut(s)	913, 1104
TaiI	ACGT	4 cut(s)	364, 912, 1986, 2322
TaqI	TCGA	8 cut(s)	56, 274, 810, 1153, 1511, 1572, 1905, 2094
TaqII	GACCGA	1 cut(s)	327
TatI	WGTACW	4 cut(s)	16, 38, 828, 1536
TauI	GCSGC	1 cut(s)	1103
TfiI	GAWTC	1 cut(s)	437
Tru1I	TTAA	4 cut(s)	104, 237, 1677, 1854
Tru9I	TTAA	4 cut(s)	104, 237, 1677, 1854
TscAI	CASTG	5 cut(s)	312, 983, 1054, 1146, 1951
TseFI	GTSAC	2 cut(s)	677, 1141
TseI	GCWGC	8 cut(s)	229, 419, 422, 425, 965, 1059, 1084, 2279
Tsp45I	GTSAC	2 cut(s)	677, 1141
TspDTI	ATGAA	8 cut(s)	147, 1086, 1472, 1716, 1830, 2064, 2328, 2414
TspRI	CASTG	5 cut(s)	312, 983, 1054, 1146, 1951
VneI	GTGCAC	1 cut(s)	1868
VpaK11BI	GGWCC	1 cut(s)	1921
XapI	RAATTY	2 cut(s)	817, 1715
XbaI	TCTAGA	1 cut(s)	2188
XceI	RCATGY	1 cut(s)	1330
XcmI	CCANNNNNNNNNTGG	1 cut(s)	920
XmiI	GTMKAC	2 cut(s)	810, 1053
XspI	CTAG	4 cut(s)	393, 555, 2189, 2336
ZraI	GACGTC	1 cut(s)	910
ZrmI	AGTACT	2 cut(s)	40, 1538
Zsp2I	ATGCAT	1 cut(s)	412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.