Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0003934

Physical Location & Seq

Genomic Coordinates Forward (+)

32848891 .. 32851906

Gene Structure 3016 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG274700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2535 bp

ATGTTCTTCAATAATAGAGATTTTTCTATGTTGATTGATCAGTCTAGCTATCGAATTCCTAGCTCCCTCATGGCTTCTTCTTCTGTACTGGTTATATTCTTACTCAGTACTGTTTTGATTATTGTCTCGATCATGCCCAATGTAAAAGCTCAAAGAAACCACTCCAAATCCAATTTAATCAGCCTGGGCTCCTCTCTATCCCCCAATGTTGATAACCTCAGTATTTCTTCATGGTTGTCATCTTCTGGCCGTTTCGCCTTCGGTTTCTACCCACAAGGCAACGGCTTTGCTGTTGGTATACGGCTTCTTAATCCACCAAACAACACAGTAGTGTGGACTGCAAATCGAGATGACCCCCCTGTCTCCCCAAACTCTACACTGAAACTAACAAGAGACGGTCTCTTGCTTCGGTCAGAGGGAGGCCAAGAAATACGTATTACTAATGGCTCAAGTTCACTAGAAACTAGTGCGATATCAAATGCATCAACGGAGGCTGCAGCTATGCGTGATTCCGGAAACTTTGTGCTGTATAATGAGACTTTTTTTGTTATATGGGAGAGCTTTGATTTCCCAACTGACACAATACTGGGAGGCCAAGATTTGGAGGCAGACATGGCTTTGGTATCTAGTGCATCTGCTACAGACCACTCCAGCGGGCGATTTTCCCTGAAGATGCAGGATGATGGAAACCTTGTGGCCTACCCGCCAAACAGTTCTTCTGCTTATTGGGCTACTATGACCTTCGATAGTTACCAGCAACTTAGTCTCAACCTATCAGGCTTTCTCGTTTTGAGTCGCCAATCCAGTAGTCAGACTACTCAAGACAATAAAACCACAATCTACCGAGCAACACTTGATGTCGATGGGATTTTCAGATTGTACTTGCATAACTTTCTGATGTCCGGTAGTCCAGAGGGAGCTGTAAAGGCTATTTGGTCAGCTTTGCAAGATCCATGCGACGTCAAAGGCATTTGTGGCTTCAACAGTTATTGTGAAGTAGTCATGCAAAAGAAAGCAGCTGAAGTTCACTGCAAATGTTACCCTGGGTTTGTTTACAACAACGCCAGTCAGATGTCTCTGGGGTGCTACCATAATTTCACTGTAGACGGTTGCACCGGAAATGAGAAGCCACGGCTGCGGTACAAGGTAGAGGCCTTGGACAATACATCGTGGATTAATCATCCTTATTCAGTGATACCTATTCAAAACAAGGAAGATTGCAGTGACTCTTGCCTGGAAGACTGCAACTGTTGGGCTGTGCTATATACAGATGCTAATTGCAGTAAATACAAGCCTCCACTTTTATATGGCAAAACAAATTCAAGTATATCCTCTAAGGGTTTCTTCAAAGTGCTTCTACCGAGAAATATGGATGGCATGCCAAACAATTTTCCCGAACTAACCGAAAGCCTGACGGTAACTGAAAACAAGAATAATAGTCTAATTCTAATTCTTGCCATCACTCTGGGTTCCATTGCATTCTTGTGTTTTGTCCTTGCCATCTCGAGTTTCACCATATACAAGCACCGAGTTCATAGGTACGAGAAGCTTTTGAAACATTCGAGTTTGGCATTAGCTGATCAAGAGTACTTCACTTTACAATCGTTTTCTTATGCTGTGCTCGAAAGAGCAACAGATGGCTTCAAGGAAGAGATAGGAAGGGGTACTTTCGGAGCTGTTTATAAAGGAACTCTGTCCGGGGCCAGCTCTAACAAAACTGTTGCTGTTAAAAGACTGGAGAAAGTGGTTGATGAAGGAGTAAGGGAATTTATAGCTGAAATTACCACCATTGGACGGACTCACCACAGAAACTTAGTTCAGTTGCTTGGTTTCTGTATTGAGGGTTCTCGGAAGCTTCTTGTTTATGAATACATGAGCAACGGCTCACTTGCGGATCTTATCTTTAAGGCCAAAAGTGTGCACTCATCTTGGAAAGAAAGGGTAAGACTTGTATTCGATGTAGCTAAAGGGGTCCTCTATTTACATGATGAGTGCAGTGTCCATATCATTCACTCCAATTTGAAACCCCAAAACGTACTCTTGGATCATACTTGGACGGCTAAGATATCTGATTTCGGTTTGGCCAGGCTGGTGCCAAATGAAACAAAAATCAACTCTACTGGAGGAGGAGAAGGAGGAGCGGGAACAGTCGAACAGGTGAGAAGAGGTTACTTGGCGCCTGAATGGCAGAAGAATGCTTTCATATCTGTAAAAGCTGATATCTACAGTTTTGGTATTGTGCTTTTAGAGATTGTATGTTGTAGAAGAAACATAGAAGTAAATGTTTCAACACCAGATGAGATAATTCTTTCGAGTTGGGTCAGTAAATGCTTTGCTGCTGGAGAATTAGATAAGCTTGTGGAGGAAGATGAAAATGTAGATTTGAAGACCCTAGAAAGAATGGTGAAAGTGGGGTTGTGGTGCGTTCAAGATGATCCAGCTTTGCGTCCTTTCATGAAGAATGTAATCTTGATGTTGGAAGGGACTATGGACATACCAGTTCCACCATGTCCAGAGCTTCCCTCTCTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

844

Amino Acids

93.44

Weight (kDa)

5.95

Isoelectric Point (pI)

37.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	109 - 205	1.4e-15	D-mannose binding lectin
S_locus_glycop	PF00954	242 - 352	6e-06	S-locus glycoprotein domain
PK_Tyr_Ser-Thr	PF07714	548 - 822	1.4e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	549 - 821	3.9e-40	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003934)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23390
prunus_persica	Prupe.6G165100_v2.0.a1 Prupe.6G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0118681 RchiOBHm_Chr2g0118741 RchiOBHm_Chr2g0118761
rosa_laevigata	RLG00000018419
rosa_multiflora	Rmu_co8322353.1_g000001 Rmu_sc0001814.1_g000026 Rmu_sc0001814.1_g000032 Rmu_sc0002700.1_g000009 Rmu_sc0002700.1_g000033 Rmu_sc0002700.1_g000035 Rmu_sc0002831.1_g000014 Rmu_sc0002831.1_g000017 Rmu_sc0002831.1_g000031 Rmu_sc0004399.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124760 Rroxscaffold_2G00124850 Rroxscaffold_2G00124890 Rroxscaffold_2G00125410 Rroxscaffold_2G00125420
rosa_rugosa	Rorug02G0218400
rosa_samantha	Rh2AG274700 Rh2AG275000 Rh2AG275300 Rh2BG286500 Rh2BG286700 Rh2BG287100 Rh2DG301500 Rh2DG301700
rosa_wichuraiana	Rw2G021850 Rw2G021890 Rw2G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1683
AasI	GACNNNNNNGTC	1 cut(s)	359
AatII	GACGTC	1 cut(s)	963
AccB1I	GGYRCC	2 cut(s)	2092, 2176
AccB7I	CCANNNNNTGG	1 cut(s)	601
AccBSI	CCGCTC	1 cut(s)	2141
AccI	GTMKAC	2 cut(s)	298, 1104
AccIII	TCCGGA	1 cut(s)	512
AciI	CCGC	5 cut(s)	654, 704, 1138, 1892, 2141
AclWI	GGATC	4 cut(s)	944, 1902, 2052, 2429
AcoI	YGGCCR	2 cut(s)	247, 2082
AcsI	RAATTY	3 cut(s)	54, 1318, 1766
AcuI	CTGAAG	2 cut(s)	689, 1041
AcyI	GRCGYC	2 cut(s)	960, 2177
AfaI	GTAC	8 cut(s)	87, 109, 881, 1142, 1541, 1589, 1666, 2037
AfiI	CCNNNNNNNGG	2 cut(s)	601, 1794
AhlI	ACTAGT	1 cut(s)	464
AjnI	CCWGG	4 cut(s)	183, 1042, 1233, 2084
AjuI	GAANNNNNNNTTGG	2 cut(s)	700, 732
AleI	CACNNNNGTG	1 cut(s)	329
Alw21I	GWGCWC	2 cut(s)	1623, 1923
Alw26I	GTCTC	7 cut(s)	130, 367, 387, 404, 530, 770, 1080
Alw44I	GTGCAC	1 cut(s)	1919
AlwI	GGATC	4 cut(s)	944, 1902, 2052, 2429
Ama87I	CYCGRG	1 cut(s)	1504
Aor13HI	TCCGGA	1 cut(s)	512
AoxI	GGCC	8 cut(s)	247, 421, 592, 696, 1152, 1701, 1908, 2082
ApaLI	GTGCAC	1 cut(s)	1919
ApeKI	GCWGC	5 cut(s)	494, 497, 1016, 1135, 2336
ApoI	RAATTY	3 cut(s)	54, 1318, 1766
AseI	ATTAAT	1 cut(s)	1176
Asp700I	GAANNNNTTC	1 cut(s)	2198
AspLEI	GCGC	1 cut(s)	2179
AspS9I	GGNCC	2 cut(s)	1701, 1972
AsuC2I	CCSGG	1 cut(s)	1699
AsuHPI	GGTGA	4 cut(s)	1504, 1793, 2170, 2416
AvaI	CYCGRG	1 cut(s)	1504
AvaII	GGWCC	1 cut(s)	1972
BaeGI	GKGCMC	1 cut(s)	1923
BalI	TGGCCA	1 cut(s)	2084
BanI	GGYRCC	2 cut(s)	2092, 2176
BanII	GRGCYC	1 cut(s)	191
BbsI	GAAGAC	2 cut(s)	1245, 2393
Bbv12I	GWGCWC	2 cut(s)	1623, 1923
BbvI	GCAGC	5 cut(s)	481, 509, 1028, 1122, 2323
BccI	CCATC	6 cut(s)	677, 857, 1367, 1466, 1508, 1631
BceAI	ACGGC	6 cut(s)	234, 298, 317, 1148, 1897, 2073
BciT130I	CCWGG	4 cut(s)	185, 1044, 1235, 2086
BclI	TGATCA	2 cut(s)	37, 1579
BcnI	CCSGG	1 cut(s)	1699
BcoDI	GTCTC	7 cut(s)	130, 367, 387, 404, 530, 770, 1080
BcuI	ACTAGT	1 cut(s)	464
BfaI	CTAG	6 cut(s)	45, 60, 458, 465, 627, 2393
BfmI	CTRYAG	4 cut(s)	495, 639, 1101, 2224
BfoI	RGCGCY	1 cut(s)	2180
BglI	GCCNNNNNGGC	1 cut(s)	2185
BisI	GCNGC	5 cut(s)	495, 498, 1017, 1136, 2337
BlsI	GCNGC	5 cut(s)	496, 499, 1018, 1137, 2338
BmcAI	AGTACT	2 cut(s)	109, 1589
Bme1390I	CCNGG	5 cut(s)	185, 1044, 1235, 1699, 2086
Bme18I	GGWCC	1 cut(s)	1972
BmeT110I	CYCGRG	1 cut(s)	1504
BmgT120I	GGNCC	2 cut(s)	1701, 1972
BmiI	GGNNCC	6 cut(s)	190, 1471, 1702, 1973, 2094, 2178
BmrFI	CCNGG	5 cut(s)	185, 1044, 1235, 1699, 2086
BmrI	ACTGGG	1 cut(s)	596
BmsI	GCATC	4 cut(s)	491, 641, 663, 1261
BmuI	ACTGGG	1 cut(s)	596
BpiI	GAAGAC	2 cut(s)	1245, 2393
BplI	GAGNNNNNCTC	3 cut(s)	384, 416, 2507
BpmI	CTGGAG	4 cut(s)	634, 1757, 2142, 2361
BpuEI	CTTGAG	2 cut(s)	433, 804
BpuMI	CCSGG	1 cut(s)	1699
BsaAI	YACGTR	1 cut(s)	434
BsaHI	GRCGYC	2 cut(s)	960, 2177
BsaI	GGTCTC	1 cut(s)	404
BsaJI	CCNNGG	6 cut(s)	184, 1042, 1043, 1130, 1155, 1698
BsaWI	WCCGGW	3 cut(s)	512, 902, 1115
Bsc4I	CCNNNNNNNGG	2 cut(s)	601, 1794
Bse1I	ACTGG	7 cut(s)	93, 591, 804, 1065, 1740, 2125, 2498
Bse3DI	GCAATG	1 cut(s)	1473
BseAI	TCCGGA	1 cut(s)	512
BseBI	CCWGG	4 cut(s)	185, 1044, 1235, 2086
BseDI	CCNNGG	6 cut(s)	184, 1042, 1043, 1130, 1155, 1698
BseGI	GGATG	3 cut(s)	685, 1180, 1378
BseLI	CCNNNNNNNGG	2 cut(s)	601, 1794
BseMI	GCAATG	1 cut(s)	1473
BseMII	CTCAG	2 cut(s)	118, 232
BseNI	ACTGG	7 cut(s)	93, 591, 804, 1065, 1740, 2125, 2498
BseRI	GAGGAG	4 cut(s)	181, 2139, 2142, 2151
BseSI	GKGCMC	1 cut(s)	1923
BseXI	GCAGC	5 cut(s)	481, 509, 1028, 1122, 2323
BsgI	GTGCAG	1 cut(s)	2014
BshFI	GGCC	8 cut(s)	249, 423, 594, 698, 1154, 1703, 1910, 2084
BshNI	GGYRCC	2 cut(s)	2092, 2176
BsiHKAI	GWGCWC	2 cut(s)	1623, 1923
BsiHKCI	CYCGRG	1 cut(s)	1504
BsiSI	CCGG	4 cut(s)	513, 903, 1116, 1698
BslFI	GGGAC	1 cut(s)	2497
BslI	CCNNNNNNNGG	2 cut(s)	601, 1794
BsmAI	GTCTC	7 cut(s)	130, 367, 387, 404, 530, 770, 1080
BsmBI	CGTCTC	1 cut(s)	387
BsmFI	GGGAC	1 cut(s)	2497
BsmI	GAATGC	2 cut(s)	1478, 2200
BsnI	GGCC	8 cut(s)	249, 423, 594, 698, 1154, 1703, 1910, 2084
Bso31I	GGTCTC	1 cut(s)	404
BsoBI	CYCGRG	1 cut(s)	1504
Bsp1286I	GDGCHC	3 cut(s)	191, 1623, 1923
Bsp13I	TCCGGA	1 cut(s)	512
Bsp143I	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
BspACI	CCGC	5 cut(s)	654, 704, 1138, 1892, 2141
BspANI	GGCC	8 cut(s)	249, 423, 594, 698, 1154, 1703, 1910, 2084
BspCNI	CTCAG	2 cut(s)	117, 231
BspEI	TCCGGA	1 cut(s)	512
BspHI	TCATGA	1 cut(s)	2454
BspLI	GGNNCC	6 cut(s)	190, 1471, 1702, 1973, 2094, 2178
BspMAI	CTGCAG	1 cut(s)	499
BspPI	GGATC	4 cut(s)	944, 1902, 2052, 2429
BspT107I	GGYRCC	2 cut(s)	2092, 2176
BspTNI	GGTCTC	1 cut(s)	404
BsrBI	CCGCTC	1 cut(s)	2141
BsrDI	GCAATG	1 cut(s)	1473
BsrI	ACTGG	7 cut(s)	93, 591, 804, 1065, 1740, 2125, 2498
BssECI	CCNNGG	6 cut(s)	184, 1042, 1043, 1130, 1155, 1698
BssMI	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
BssNAI	GTATAC	1 cut(s)	299
BssNI	GRCGYC	2 cut(s)	960, 2177
BssT1I	CCWWGG	1 cut(s)	1155
Bst1107I	GTATAC	1 cut(s)	299
Bst2UI	CCWGG	4 cut(s)	185, 1044, 1235, 2086
Bst6I	CTCTTC	2 cut(s)	1644, 2158
BstACI	GRCGYC	2 cut(s)	960, 2177
BstBAI	YACGTR	1 cut(s)	434
BstC8I	GCNNGC	3 cut(s)	656, 1379, 1705
BstDEI	CTNAG	6 cut(s)	104, 218, 761, 1335, 1813, 2061
BstDSI	CCRYGG	1 cut(s)	1130
BstF5I	GGATG	3 cut(s)	685, 1180, 1378
BstH2I	RGCGCY	1 cut(s)	2180
BstHHI	GCGC	1 cut(s)	2179
BstKTI	GATC	7 cut(s)	40, 132, 952, 1582, 1897, 2047, 2437
BstMAI	GTCTC	7 cut(s)	130, 367, 387, 404, 530, 770, 1080
BstMBI	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
BstMWI	GCNNNNNNNGC	6 cut(s)	614, 728, 926, 975, 1135, 2185
BstNI	CCWGG	4 cut(s)	185, 1044, 1235, 2086
BstNSI	RCATGY	1 cut(s)	1381
BstSCI	CCNGG	5 cut(s)	183, 1042, 1233, 1697, 2084
BstSFI	CTRYAG	4 cut(s)	495, 639, 1101, 2224
BstSLI	GKGCMC	1 cut(s)	1923
BstSNI	TACGTA	1 cut(s)	434
BstV1I	GCAGC	5 cut(s)	481, 509, 1028, 1122, 2323
BstV2I	GAAGAC	2 cut(s)	1245, 2393
BstX2I	RGATCY	2 cut(s)	949, 1894
BstXI	CCANNNNNNTGG	1 cut(s)	1465
BstYI	RGATCY	2 cut(s)	949, 1894
BstZ17I	GTATAC	1 cut(s)	299
BsuRI	GGCC	8 cut(s)	249, 423, 594, 698, 1154, 1703, 1910, 2084
BtgI	CCRYGG	1 cut(s)	1130
BtsCI	GGATG	3 cut(s)	685, 1180, 1378
BtsI	GCAGTG	3 cut(s)	1027, 1228, 2002
BtsIMutI	CAGTG	6 cut(s)	377, 1027, 1098, 1197, 1228, 2002
Cac8I	GCNNGC	3 cut(s)	656, 1379, 1705
CciI	TCATGA	1 cut(s)	2454
CfoI	GCGC	1 cut(s)	2179
Cfr13I	GGNCC	2 cut(s)	1701, 1972
CseI	GACGC	1 cut(s)	2435
Csp6I	GTAC	8 cut(s)	86, 108, 880, 1141, 1540, 1588, 1665, 2036
CviQI	GTAC	8 cut(s)	86, 108, 880, 1141, 1540, 1588, 1665, 2036
DdeI	CTNAG	6 cut(s)	104, 218, 761, 1335, 1813, 2061
DinI	GGCGCC	1 cut(s)	2178
DpnI	GATC	7 cut(s)	39, 131, 951, 1581, 1896, 2046, 2436
DpnII	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
DrdI	GACNNNNNNGTC	1 cut(s)	359
DseDI	GACNNNNNNGTC	1 cut(s)	359
EaeI	YGGCCR	2 cut(s)	247, 2082
Eam1104I	CTCTTC	2 cut(s)	1644, 2158
EarI	CTCTTC	2 cut(s)	1644, 2158
Eco105I	TACGTA	1 cut(s)	434
Eco130I	CCWWGG	1 cut(s)	1155
Eco147I	AGGCCT	1 cut(s)	1154
Eco24I	GRGCYC	1 cut(s)	191
Eco31I	GGTCTC	1 cut(s)	404
Eco32I	GATATC	3 cut(s)	474, 2067, 2221
Eco47I	GGWCC	1 cut(s)	1972
Eco57I	CTGAAG	2 cut(s)	689, 1041
Eco88I	CYCGRG	1 cut(s)	1504
EcoO109I	RGGNCCY	1 cut(s)	1972
EcoRI	GAATTC	1 cut(s)	54
EcoRII	CCWGG	4 cut(s)	183, 1042, 1233, 2084
EcoRV	GATATC	3 cut(s)	474, 2067, 2221
EcoT14I	CCWWGG	1 cut(s)	1155
EcoT22I	ATGCAT	1 cut(s)	484
EcoT38I	GRGCYC	1 cut(s)	191
EgeI	GGCGCC	1 cut(s)	2178
EheI	GGCGCC	1 cut(s)	2178
ErhI	CCWWGG	1 cut(s)	1155
Esp3I	CGTCTC	1 cut(s)	387
FalI	AAGNNNNNCTT	4 cut(s)	1328, 1360, 1651, 1683
FaqI	GGGAC	1 cut(s)	2497
FauI	CCCGC	3 cut(s)	647, 711, 2134
FbaI	TGATCA	2 cut(s)	37, 1579
FblI	GTMKAC	2 cut(s)	298, 1104
Fnu4HI	GCNGC	5 cut(s)	495, 498, 1017, 1136, 2337
FokI	GGATG	3 cut(s)	692, 1167, 1385
FriOI	GRGCYC	1 cut(s)	191
Fsp4HI	GCNGC	5 cut(s)	495, 498, 1017, 1136, 2337
FspBI	CTAG	6 cut(s)	45, 60, 458, 465, 627, 2393
GlaI	GCGC	1 cut(s)	2178
GluI	GCNGC	5 cut(s)	495, 498, 1017, 1136, 2337
GsuI	CTGGAG	4 cut(s)	634, 1757, 2142, 2361
HaeII	RGCGCY	1 cut(s)	2180
HaeIII	GGCC	8 cut(s)	249, 423, 594, 698, 1154, 1703, 1910, 2084
HapII	CCGG	4 cut(s)	513, 903, 1116, 1698
HgaI	GACGC	1 cut(s)	2435
HhaI	GCGC	1 cut(s)	2179
Hin1I	GRCGYC	2 cut(s)	960, 2177
Hin6I	GCGC	1 cut(s)	2177
HinP1I	GCGC	1 cut(s)	2177
HindIII	AAGCTT	3 cut(s)	1547, 1853, 2354
HinfI	GANTC	4 cut(s)	509, 793, 1226, 1798
HpaII	CCGG	4 cut(s)	513, 903, 1116, 1698
HphI	GGTGA	4 cut(s)	1504, 1793, 2170, 2416
Hpy166II	GTNNAC	7 cut(s)	299, 336, 455, 1027, 1054, 1105, 1921
Hpy188I	TCNGA	8 cut(s)	415, 813, 875, 897, 1071, 1673, 1851, 2071
Hpy8I	GTNNAC	7 cut(s)	299, 336, 455, 1027, 1054, 1105, 1921
Hpy99I	CGWCG	1 cut(s)	962
HpyAV	CCTTC	6 cut(s)	268, 751, 1653, 1748, 2126, 2474
HpyCH4IV	ACGT	3 cut(s)	433, 960, 2034
HpyF10VI	GCNNNNNNNGC	6 cut(s)	614, 728, 926, 975, 1135, 2185
HpyF3I	CTNAG	6 cut(s)	104, 218, 761, 1335, 1813, 2061
HpySE526I	ACGT	3 cut(s)	433, 960, 2034
Hsp92I	GRCGYC	2 cut(s)	960, 2177
HspAI	GCGC	1 cut(s)	2177
KasI	GGCGCC	1 cut(s)	2176
Kpn2I	TCCGGA	1 cut(s)	512
Ksp22I	TGATCA	2 cut(s)	37, 1579
Kzo9I	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
LmnI	GCTCC	5 cut(s)	68, 194, 917, 1673, 2138
Lsp1109I	GCAGC	5 cut(s)	481, 509, 1028, 1122, 2323
LweI	GCATC	4 cut(s)	491, 641, 663, 1261
MaeI	CTAG	6 cut(s)	45, 60, 458, 465, 627, 2393
MaeII	ACGT	3 cut(s)	433, 960, 2034
MaeIII	GTNAC	5 cut(s)	749, 1037, 1223, 1417, 2168
MalI	GATC	7 cut(s)	39, 131, 951, 1581, 1896, 2046, 2436
MbiI	CCGCTC	1 cut(s)	2141
MboI	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
MflI	RGATCY	2 cut(s)	949, 1894
MhlI	GDGCHC	3 cut(s)	191, 1623, 1923
MlsI	TGGCCA	1 cut(s)	2084
MluNI	TGGCCA	1 cut(s)	2084
Mly113I	GGCGCC	1 cut(s)	2177
MlyI	GAGTC	3 cut(s)	802, 1220, 1792
MmeI	TCCRAC	1 cut(s)	2457
Mox20I	TGGCCA	1 cut(s)	2084
Mph1103I	ATGCAT	1 cut(s)	484
MroI	TCCGGA	1 cut(s)	512
MroXI	GAANNNNTTC	1 cut(s)	2198
MscI	TGGCCA	1 cut(s)	2084
MseI	TTAA	5 cut(s)	176, 309, 1176, 1728, 1905
MslI	CAYNNNNRTG	2 cut(s)	329, 1483
Msp20I	TGGCCA	1 cut(s)	2084
MspA1I	CMGCKG	2 cut(s)	654, 1019
MspI	CCGG	4 cut(s)	513, 903, 1116, 1698
MspR9I	CCNGG	5 cut(s)	185, 1044, 1235, 1699, 2086
Mva1269I	GAATGC	2 cut(s)	1478, 2200
MvaI	CCWGG	4 cut(s)	185, 1044, 1235, 2086
MwoI	GCNNNNNNNGC	6 cut(s)	614, 728, 926, 975, 1135, 2185
NarI	GGCGCC	1 cut(s)	2177
NciI	CCSGG	1 cut(s)	1699
NdeII	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
NlaIV	GGNNCC	6 cut(s)	190, 1471, 1702, 1973, 2094, 2178
NmuCI	GTSAC	1 cut(s)	1223
NsiI	ATGCAT	1 cut(s)	484
NspI	RCATGY	1 cut(s)	1381
OliI	CACNNNNGTG	1 cut(s)	329
PaeI	GCATGC	1 cut(s)	1381
PaeR7I	CTCGAG	1 cut(s)	1504
PagI	TCATGA	1 cut(s)	2454
PasI	CCCWGGG	1 cut(s)	1043
PceI	AGGCCT	1 cut(s)	1154
PctI	GAATGC	2 cut(s)	1478, 2200
PdmI	GAANNNNTTC	1 cut(s)	2198
PfeI	GAWTC	1 cut(s)	509
PflMI	CCANNNNNTGG	1 cut(s)	601
PkrI	GCNGC	5 cut(s)	496, 499, 1018, 1137, 2338
PleI	GAGTC	3 cut(s)	801, 1220, 1792
PluTI	GGCGCC	1 cut(s)	2180
PpsI	GAGTC	3 cut(s)	801, 1220, 1792
Ppu21I	YACGTR	1 cut(s)	434
PpuMI	RGGWCCY	1 cut(s)	1972
PshBI	ATTAAT	1 cut(s)	1176
PsiI	TTATAA	1 cut(s)	1683
Psp5II	RGGWCCY	1 cut(s)	1972
Psp6I	CCWGG	4 cut(s)	183, 1042, 1233, 2084
PspGI	CCWGG	4 cut(s)	183, 1042, 1233, 2084
PspN4I	GGNNCC	6 cut(s)	190, 1471, 1702, 1973, 2094, 2178
PspPI	GGNCC	2 cut(s)	1701, 1972
PspPPI	RGGWCCY	1 cut(s)	1972
PsrI	GAACNNNNNNTAC	2 cut(s)	1828, 1860
PstI	CTGCAG	1 cut(s)	499
PsuI	RGATCY	2 cut(s)	949, 1894
PvuII	CAGCTG	1 cut(s)	1019
RsaI	GTAC	8 cut(s)	87, 109, 881, 1142, 1541, 1589, 1666, 2037
RsaNI	GTAC	8 cut(s)	86, 108, 880, 1141, 1540, 1588, 1665, 2036
RseI	CAYNNNNRTG	2 cut(s)	329, 1483
SaqAI	TTAA	5 cut(s)	176, 309, 1176, 1728, 1905
SatI	GCNGC	5 cut(s)	495, 498, 1017, 1136, 2337
Sau3AI	GATC	7 cut(s)	37, 129, 949, 1579, 1894, 2044, 2434
Sau96I	GGNCC	2 cut(s)	1701, 1972
ScaI	AGTACT	2 cut(s)	109, 1589
SchI	GAGTC	3 cut(s)	802, 1220, 1792
ScrFI	CCNGG	5 cut(s)	185, 1044, 1235, 1699, 2086
SduI	GDGCHC	3 cut(s)	191, 1623, 1923
SfaNI	GCATC	4 cut(s)	491, 641, 663, 1261
SfcI	CTRYAG	4 cut(s)	495, 639, 1101, 2224
SfoI	GGCGCC	1 cut(s)	2178
Sfr274I	CTCGAG	1 cut(s)	1504
SinI	GGWCC	1 cut(s)	1972
SlaI	CTCGAG	1 cut(s)	1504
SmiMI	CAYNNNNRTG	2 cut(s)	329, 1483
SmlI	CTYRAG	3 cut(s)	448, 819, 1504
SmoI	CTYRAG	3 cut(s)	448, 819, 1504
SnaBI	TACGTA	1 cut(s)	434
SpeI	ACTAGT	1 cut(s)	464
SphI	GCATGC	1 cut(s)	1381
SseBI	AGGCCT	1 cut(s)	1154
SsiI	CCGC	5 cut(s)	654, 704, 1138, 1892, 2141
SspDI	GGCGCC	1 cut(s)	2176
SspMI	CTAG	6 cut(s)	45, 60, 458, 465, 627, 2393
StuI	AGGCCT	1 cut(s)	1154
StyD4I	CCNGG	5 cut(s)	183, 1042, 1233, 1697, 2084
StyI	CCWWGG	1 cut(s)	1155
TaiI	ACGT	3 cut(s)	436, 963, 2037
TaqII	GACCGA	1 cut(s)	399
TatI	WGTACW	4 cut(s)	85, 107, 879, 1587
TfiI	GAWTC	1 cut(s)	509
Tru1I	TTAA	5 cut(s)	176, 309, 1176, 1728, 1905
Tru9I	TTAA	5 cut(s)	176, 309, 1176, 1728, 1905
TscAI	CASTG	6 cut(s)	384, 1034, 1105, 1197, 1228, 2002
TseFI	GTSAC	1 cut(s)	1223
TseI	GCWGC	5 cut(s)	494, 497, 1016, 1135, 2336
Tsp45I	GTSAC	1 cut(s)	1223
TspDTI	ATGAA	9 cut(s)	219, 1523, 1767, 1881, 2115, 2191, 2385, 2443, 2471
TspGWI	ACGGA	2 cut(s)	503, 1810
TspRI	CASTG	6 cut(s)	384, 1034, 1105, 1197, 1228, 2002
Van91I	CCANNNNNTGG	1 cut(s)	601
VneI	GTGCAC	1 cut(s)	1919
VpaK11BI	GGWCC	1 cut(s)	1972
VspI	ATTAAT	1 cut(s)	1176
XapI	RAATTY	3 cut(s)	54, 1318, 1766
XceI	RCATGY	1 cut(s)	1381
XhoI	CTCGAG	1 cut(s)	1504
XmiI	GTMKAC	2 cut(s)	298, 1104
XmnI	GAANNNNTTC	1 cut(s)	2198
XspI	CTAG	6 cut(s)	45, 60, 458, 465, 627, 2393
ZraI	GACGTC	1 cut(s)	961
ZrmI	AGTACT	2 cut(s)	109, 1589
Zsp2I	ATGCAT	1 cut(s)	484

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.