Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003934

Physical Location & Seq

Genomic Coordinates Forward (+)

30455396 .. 30466233

Gene Structure 10838 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G021850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGGCTTCTTCTTCTTCTGTACTGGTTATATTCTTACTCAGTACTGATTTGATTATCTCGATCATGCCCAATGTAAACGCGCAAAGAAACCACTCCAAATCCAATTTAATCAGCCCGGGCTCCACTCTATCCCCCCATCGCTATAACCTCAGTATTTCTTCATGGTTGTCATCTTCTGGCCGTTTCGCCTTCGGTTTCTACCCACATGGTAACGGCTTTGCGGTTGGTATACGGCTTCTTAATCCACCAAACAACACAATACCCAAGAATTCTGCTCGAGGGGACCCCCCTGTCTCCTCAAACTCTACACTGAAACTTACGGGAGACGGTCTCTTGCTTCGGTCAGAGGGAGGCCAAGAAATACCTATTACTAATAGCTCAAATTTACAAGAAACTAGCGCGATATCAAATGCATCAACTGCCGCTGCAGCTATGCTTGATTCCGGAAACTTTGTGCTGTATGATGAGACTTTTTTTGTTATATGGGAGAGCTTTGATTTCCCAACTGATACAATCCTGGGAGGTCAAGATTTGCTGGCGTCCATGGGTCTGATATCTAGTGCATCTGTTGCAGACCACTCCAGCGGGCGATTTTCCCTGGAGATGCAGGATGATGGAAACCTTGTGGCCTACCCGCCAAACAGTTCTTCTCCTTATTTGGCTACTATGACCTTCGGTAGTTACCAACTTAGTCTCAACCTATCAGGCTTTCTCGTTTTGAGTAGCCAATCCAGTGGTCTGACTACTCAGGTCTTGGCAAACGGCTCGTACCATCACCCACAAGACAATAAAACCACAATCTACCGAGCAACACTTGATGTCGATGGGATTTTCAGATTGTACTTGCATAACTTTCTGATGTCCGGTAGTCCAGAGGGAGCTGTAAAGGCTATTTGGTCAGCTTTGCAAGATCCATGCGACGTCAAAGGCATTTGTGGCTTCAACAGTTATTGTGAAGTAGTCATGCAAAAGAAAGCAGCTGAAGTTCACTGCAAATGTTACCCTGGATTTGTTTACAACAACGCCAGTCAGATGTCTCTGGGGTGCTATCATAATTTCACTGTAGGAGGCTGCACCGGAAATGAAGAGCCACGGCTGCGGTACAAGGTAGCGGCCTTGGACAATACATCGTGGAGTAATCATCCTTATTCAGTGACACCTATTCAAAACAAGGAAGATTGCGGTGACTCTTGCCTGGGAGACTGCAATTGTTGGGCTGTGCTATATACAGATGCTGATTGCAGAAAATACAAGCTTCCACTTTTATATGGCAAAACAACTTCAAGTATATCCTCTAAAGGTTTCATCAAAGTGCTTCTACCGAGAAACATGGACAGTATGCCAAATAATTTTCCCTACCTACCCAAAAGTCCGACGGTAACTGAAAGCAAGAATAAGAGTCTAATTCTAATTCTTGCTATCAGTCTGGGTTCCATTGCATTCTTGTGTTCTGTCTTTGCCATCTCGAGTTTCGCCATATACATGCACCGAGTTCATAGGTACAAGAAGCTGTTGAAACATTCGAGTTTGGCATTAGCTGATCAAGAGTACTTCACTTTGCAATCATTTTCTTACGCTGAGCTAGAAAGAGCAACAGATGGCTTCAAGGAAGAGATAGGAAGGGGTACTTTCGGAGCTGTTTATAAAGGAACCCTGTCCGTGGGCAGCTCTAACAAAACTGTTGCTGTTAAAAGACTGGAGAAAGTGGTTGATGAAGGAGTAAGGGAATTTATAGCAGAAATGACCACCATTGGACGAACTCACCACAGAAACTTAATTCAGTTGCTTGGTTTCTGTACTGAGGGTTCTCGGAAGCTTCTTGTTTATGAATACATGAGCCACGGCTCACTTGCGGATCTTATCTTTAAGGCTAAAAGTGTTCACTCATCTTGGAAAGAAAGGGTAAGACTTGTATTTGATGTGGCTAAAGGGGTCCTCTATTTACATGATGAGTGCAGTGTCCATATCATTCACTCCAATTTGAAACCCCAAAACGTACTCTTGGATCATACTTGGATGGCTAAGATATCTGATTTCGGTTTGGCCAGGCTGGTGCCAAATGAAACAAAAATCAACTCTACTGGAGGAGAAGGAGGAGGAGCGGGAACAGTCGAACAGCTGAGAAGAGGTTACTTGGCGCCTGAATGGCAGAAGAATGCTCTCATATCTGTAAAAGCTGATATCTACAGTTTTGGTATTGTGCTTTTAGAGATTTTATGTTGTAGAAGAAACATAGAAGTAAATGTTTCAACAACAGACGAGATAATTCTTTCGAGTTGGGTCAGTAAGTGCTTTACTGCTGGAGAATTAGATAAGCTTGTGGAGGACGATGAAAATGTAGATTTGAAAACCCTAGAAAGAATGGTGAAAGTGGGGTTGTGGTGCGTTCAAGATGATCCAGCTTTGCGTCCTTTCATGAAGAATATAATCTTGATGTTGGAAGGGACAATGGACATACCAGTTCCACCATGTCCAGAGCTTCCCTCTCTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

831

Amino Acids

91.25

Weight (kDa)

6.41

Isoelectric Point (pI)

40.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	97 - 179	7.1e-10	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	535 - 810	9.3e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	536 - 809	6.2e-40	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003934)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23390
prunus_persica	Prupe.6G165100_v2.0.a1 Prupe.6G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0118681 RchiOBHm_Chr2g0118741 RchiOBHm_Chr2g0118761
rosa_laevigata	RLG00000018419
rosa_multiflora	Rmu_co8322353.1_g000001 Rmu_sc0001814.1_g000026 Rmu_sc0001814.1_g000032 Rmu_sc0002700.1_g000009 Rmu_sc0002700.1_g000033 Rmu_sc0002700.1_g000035 Rmu_sc0002831.1_g000014 Rmu_sc0002831.1_g000017 Rmu_sc0002831.1_g000031 Rmu_sc0004399.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124760 Rroxscaffold_2G00124850 Rroxscaffold_2G00124890 Rroxscaffold_2G00125410 Rroxscaffold_2G00125420
rosa_rugosa	Rorug02G0218400
rosa_samantha	Rh2AG274700 Rh2AG275000 Rh2AG275300 Rh2BG286500 Rh2BG286700 Rh2BG287100 Rh2DG301500 Rh2DG301700
rosa_wichuraiana	Rw2G021850 Rw2G021890 Rw2G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1644
AasI	GACNNNNNNGTC	1 cut(s)	290
AatII	GACGTC	1 cut(s)	924
AccB1I	GGYRCC	2 cut(s)	2053, 2137
AccBSI	CCGCTC	1 cut(s)	2102
AccI	GTMKAC	1 cut(s)	229
AccII	CGCG	2 cut(s)	80, 401
AccIII	TCCGGA	1 cut(s)	443
AciI	CCGC	9 cut(s)	221, 423, 585, 635, 1099, 1112, 1182, 1853, 2102
AclWI	GGATC	4 cut(s)	905, 1863, 2013, 2390
AcoI	YGGCCR	2 cut(s)	178, 2043
AcsI	RAATTY	3 cut(s)	268, 382, 1727
AcuI	CTGAAG	1 cut(s)	1002
AcyI	GRCGYC	3 cut(s)	539, 921, 2138
AfiI	CCNNNNNNNGG	1 cut(s)	1660
AgsI	TTSAA	9 cut(s)	943, 1166, 1284, 1516, 1606, 1984, 2250, 2347, 2390
AjnI	CCWGG	5 cut(s)	516, 597, 1003, 1194, 2045
AjuI	GAANNNNNNNTTGG	2 cut(s)	631, 663
Alw26I	GTCTC	7 cut(s)	298, 318, 335, 461, 698, 1041, 1194
AlwI	GGATC	4 cut(s)	905, 1863, 2013, 2390
AlwNI	CAGNNNCTG	1 cut(s)	1235
Ama87I	CYCGRG	3 cut(s)	115, 276, 1465
Aor13HI	TCCGGA	1 cut(s)	443
AoxI	GGCC	5 cut(s)	178, 352, 627, 1113, 2043
ApeKI	GCWGC	6 cut(s)	425, 428, 977, 1071, 1096, 1665
ApoI	RAATTY	3 cut(s)	268, 382, 1727
AspLEI	GCGC	3 cut(s)	82, 401, 2140
AspS9I	GGNCC	2 cut(s)	283, 1933
AsuC2I	CCSGG	2 cut(s)	116, 117
AsuHPI	GGTGA	4 cut(s)	767, 1196, 1754, 2377
AvaI	CYCGRG	3 cut(s)	115, 276, 1465
AvaII	GGWCC	2 cut(s)	283, 1933
BalI	TGGCCA	1 cut(s)	2045
BanI	GGYRCC	2 cut(s)	2053, 2137
BanII	GRGCYC	1 cut(s)	122
BbvI	GCAGC	6 cut(s)	412, 440, 989, 1058, 1083, 1677
BccI	CCATC	7 cut(s)	144, 608, 780, 818, 1469, 1592, 2011
BceAI	ACGGC	6 cut(s)	165, 229, 248, 778, 1109, 1858
BcgI	CGANNNNNNTGC	2 cut(s)	747, 781
BciT130I	CCWGG	5 cut(s)	518, 599, 1005, 1196, 2047
BclI	TGATCA	1 cut(s)	1540
BcnI	CCSGG	2 cut(s)	116, 117
BcoDI	GTCTC	7 cut(s)	298, 318, 335, 461, 698, 1041, 1194
BfaI	CTAG	4 cut(s)	396, 558, 1583, 2354
BfmI	CTRYAG	3 cut(s)	426, 1062, 2185
BfoI	RGCGCY	1 cut(s)	2141
BglI	GCCNNNNNGGC	1 cut(s)	2146
BisI	GCNGC	8 cut(s)	423, 426, 429, 978, 1072, 1097, 1113, 1666
BlpI	GCTNAGC	1 cut(s)	1578
BlsI	GCNGC	8 cut(s)	424, 427, 430, 979, 1073, 1098, 1114, 1667
BmcAI	AGTACT	2 cut(s)	43, 1550
Bme1390I	CCNGG	7 cut(s)	116, 117, 518, 599, 1005, 1196, 2047
Bme18I	GGWCC	2 cut(s)	283, 1933
BmeT110I	CYCGRG	3 cut(s)	115, 276, 1465
BmgT120I	GGNCC	2 cut(s)	283, 1933
BmiI	GGNNCC	8 cut(s)	121, 284, 285, 1432, 1651, 1934, 2055, 2139
BmrFI	CCNGG	7 cut(s)	116, 117, 518, 599, 1005, 1196, 2047
BmsI	GCATC	4 cut(s)	422, 572, 594, 1222
BplI	GAGNNNNNCTC	3 cut(s)	315, 347, 2468
BpmI	CTGGAG	5 cut(s)	565, 620, 1718, 2103, 2322
Bpu1102I	GCTNAGC	1 cut(s)	1578
BpuMI	CCSGG	2 cut(s)	116, 117
BsaHI	GRCGYC	3 cut(s)	539, 921, 2138
BsaI	GGTCTC	1 cut(s)	335
BsaWI	WCCGGW	3 cut(s)	443, 863, 1076
Bsc4I	CCNNNNNNNGG	1 cut(s)	1660
Bse1I	ACTGG	6 cut(s)	27, 732, 1026, 1701, 2086, 2459
Bse3DI	GCAATG	1 cut(s)	1434
BseAI	TCCGGA	1 cut(s)	443
BseBI	CCWGG	5 cut(s)	518, 599, 1005, 1196, 2047
BseGI	GGATG	3 cut(s)	616, 1141, 2022
BseLI	CCNNNNNNNGG	1 cut(s)	1660
BseMI	GCAATG	1 cut(s)	1434
BseMII	CTCAG	6 cut(s)	52, 163, 761, 1569, 1791, 2111
BseNI	ACTGG	6 cut(s)	27, 732, 1026, 1701, 2086, 2459
BseRI	GAGGAG	4 cut(s)	286, 2100, 2109, 2112
BseXI	GCAGC	6 cut(s)	412, 440, 989, 1058, 1083, 1677
BsgI	GTGCAG	2 cut(s)	1057, 1975
Bsh1236I	CGCG	2 cut(s)	80, 401
BshFI	GGCC	5 cut(s)	180, 354, 629, 1115, 2045
BshNI	GGYRCC	2 cut(s)	2053, 2137
BsiHKCI	CYCGRG	3 cut(s)	115, 276, 1465
BsiSI	CCGG	4 cut(s)	116, 444, 864, 1077
BslFI	GGGAC	2 cut(s)	296, 2458
BslI	CCNNNNNNNGG	1 cut(s)	1660
BsmAI	GTCTC	7 cut(s)	298, 318, 335, 461, 698, 1041, 1194
BsmBI	CGTCTC	1 cut(s)	318
BsmFI	GGGAC	2 cut(s)	296, 2458
BsmI	GAATGC	2 cut(s)	1439, 2161
BsnI	GGCC	5 cut(s)	180, 354, 629, 1115, 2045
Bso31I	GGTCTC	1 cut(s)	335
BsoBI	CYCGRG	3 cut(s)	115, 276, 1465
Bsp1286I	GDGCHC	1 cut(s)	122
Bsp13I	TCCGGA	1 cut(s)	443
Bsp143I	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
Bsp1720I	GCTNAGC	1 cut(s)	1578
Bsp19I	CCATGG	1 cut(s)	543
BspACI	CCGC	9 cut(s)	221, 423, 585, 635, 1099, 1112, 1182, 1853, 2102
BspANI	GGCC	5 cut(s)	180, 354, 629, 1115, 2045
BspCNI	CTCAG	6 cut(s)	51, 162, 760, 1570, 1792, 2112
BspEI	TCCGGA	1 cut(s)	443
BspFNI	CGCG	2 cut(s)	80, 401
BspHI	TCATGA	1 cut(s)	2415
BspLI	GGNNCC	8 cut(s)	121, 284, 285, 1432, 1651, 1934, 2055, 2139
BspMAI	CTGCAG	1 cut(s)	430
BspPI	GGATC	4 cut(s)	905, 1863, 2013, 2390
BspQI	GCTCTTC	1 cut(s)	1080
BspT107I	GGYRCC	2 cut(s)	2053, 2137
BspTNI	GGTCTC	1 cut(s)	335
BsrBI	CCGCTC	1 cut(s)	2102
BsrDI	GCAATG	1 cut(s)	1434
BsrI	ACTGG	6 cut(s)	27, 732, 1026, 1701, 2086, 2459
BssMI	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
BssNAI	GTATAC	1 cut(s)	230
BssNI	GRCGYC	3 cut(s)	539, 921, 2138
BssT1I	CCWWGG	2 cut(s)	543, 1116
Bst1107I	GTATAC	1 cut(s)	230
Bst2UI	CCWGG	5 cut(s)	518, 599, 1005, 1196, 2047
Bst4CI	ACNGT	9 cut(s)	329, 644, 947, 1063, 1337, 1378, 1681, 2110, 2189
Bst6I	CTCTTC	3 cut(s)	1080, 1605, 2119
BstACI	GRCGYC	3 cut(s)	539, 921, 2138
BstAPI	GCANNNNNTGC	2 cut(s)	419, 569
BstC8I	GCNNGC	2 cut(s)	537, 587
BstDEI	CTNAG	8 cut(s)	38, 149, 689, 747, 1578, 1800, 2022, 2120
BstDSI	CCRYGG	4 cut(s)	543, 1091, 1659, 1840
BstF5I	GGATG	3 cut(s)	616, 1141, 2022
BstFNI	CGCG	2 cut(s)	80, 401
BstH2I	RGCGCY	1 cut(s)	2141
BstHHI	GCGC	3 cut(s)	82, 401, 2140
BstKTI	GATC	6 cut(s)	63, 913, 1543, 1858, 2008, 2398
BstMAI	GTCTC	7 cut(s)	298, 318, 335, 461, 698, 1041, 1194
BstMBI	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
BstMWI	GCNNNNNNNGC	7 cut(s)	419, 428, 569, 887, 936, 1096, 2146
BstNI	CCWGG	5 cut(s)	518, 599, 1005, 1196, 2047
BstNSI	RCATGY	1 cut(s)	1486
BstSCI	CCNGG	7 cut(s)	114, 115, 516, 597, 1003, 1194, 2045
BstSFI	CTRYAG	3 cut(s)	426, 1062, 2185
BstUI	CGCG	2 cut(s)	80, 401
BstV1I	GCAGC	6 cut(s)	412, 440, 989, 1058, 1083, 1677
BstX2I	RGATCY	2 cut(s)	910, 1855
BstXI	CCANNNNNNTGG	1 cut(s)	734
BstYI	RGATCY	2 cut(s)	910, 1855
BstZ17I	GTATAC	1 cut(s)	230
BsuRI	GGCC	5 cut(s)	180, 354, 629, 1115, 2045
BtgI	CCRYGG	4 cut(s)	543, 1091, 1659, 1840
BtgZI	GCGATG	1 cut(s)	122
BtsCI	GGATG	3 cut(s)	616, 1141, 2022
BtsI	GCAGTG	2 cut(s)	988, 1963
BtsIMutI	CAGTG	6 cut(s)	308, 739, 988, 1059, 1158, 1963
Cac8I	GCNNGC	2 cut(s)	537, 587
CaiI	CAGNNNCTG	1 cut(s)	1235
CciI	TCATGA	1 cut(s)	2415
CfoI	GCGC	3 cut(s)	82, 401, 2140
Cfr13I	GGNCC	2 cut(s)	283, 1933
Cfr9I	CCCGGG	1 cut(s)	115
CseI	GACGC	2 cut(s)	528, 2396
DdeI	CTNAG	8 cut(s)	38, 149, 689, 747, 1578, 1800, 2022, 2120
DinI	GGCGCC	1 cut(s)	2139
DpnI	GATC	6 cut(s)	62, 912, 1542, 1857, 2007, 2397
DpnII	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	2 cut(s)	178, 2043
Eam1104I	CTCTTC	3 cut(s)	1080, 1605, 2119
EarI	CTCTTC	3 cut(s)	1080, 1605, 2119
Eco130I	CCWWGG	2 cut(s)	543, 1116
Eco24I	GRGCYC	1 cut(s)	122
Eco31I	GGTCTC	1 cut(s)	335
Eco32I	GATATC	4 cut(s)	405, 555, 2028, 2182
Eco47I	GGWCC	2 cut(s)	283, 1933
Eco57I	CTGAAG	1 cut(s)	1002
Eco88I	CYCGRG	3 cut(s)	115, 276, 1465
EcoO109I	RGGNCCY	2 cut(s)	283, 1933
EcoRI	GAATTC	1 cut(s)	268
EcoRII	CCWGG	5 cut(s)	516, 597, 1003, 1194, 2045
EcoRV	GATATC	4 cut(s)	405, 555, 2028, 2182
EcoT14I	CCWWGG	2 cut(s)	543, 1116
EcoT22I	ATGCAT	1 cut(s)	415
EcoT38I	GRGCYC	1 cut(s)	122
EgeI	GGCGCC	1 cut(s)	2139
EheI	GGCGCC	1 cut(s)	2139
ErhI	CCWWGG	2 cut(s)	543, 1116
Esp3I	CGTCTC	1 cut(s)	318
FalI	AAGNNNNNCTT	2 cut(s)	1612, 1644
FaqI	GGGAC	2 cut(s)	296, 2458
FauI	CCCGC	3 cut(s)	578, 642, 2095
FbaI	TGATCA	1 cut(s)	1540
FblI	GTMKAC	1 cut(s)	229
Fnu4HI	GCNGC	8 cut(s)	423, 426, 429, 978, 1072, 1097, 1113, 1666
FokI	GGATG	3 cut(s)	623, 1128, 2029
FriOI	GRGCYC	1 cut(s)	122
Fsp4HI	GCNGC	8 cut(s)	423, 426, 429, 978, 1072, 1097, 1113, 1666
FspBI	CTAG	4 cut(s)	396, 558, 1583, 2354
GlaI	GCGC	3 cut(s)	81, 400, 2139
GluI	GCNGC	8 cut(s)	423, 426, 429, 978, 1072, 1097, 1113, 1666
GsuI	CTGGAG	5 cut(s)	565, 620, 1718, 2103, 2322
HaeII	RGCGCY	1 cut(s)	2141
HaeIII	GGCC	5 cut(s)	180, 354, 629, 1115, 2045
HapII	CCGG	4 cut(s)	116, 444, 864, 1077
HgaI	GACGC	2 cut(s)	528, 2396
HhaI	GCGC	3 cut(s)	82, 401, 2140
Hin1I	GRCGYC	3 cut(s)	539, 921, 2138
Hin6I	GCGC	3 cut(s)	80, 399, 2138
HinP1I	GCGC	3 cut(s)	80, 399, 2138
HindIII	AAGCTT	3 cut(s)	1253, 1814, 2315
HinfI	GANTC	3 cut(s)	440, 1187, 1399
HpaII	CCGG	4 cut(s)	116, 444, 864, 1077
HphI	GGTGA	4 cut(s)	767, 1196, 1754, 2377
Hpy166II	GTNNAC	5 cut(s)	76, 230, 988, 1015, 1882
Hpy8I	GTNNAC	5 cut(s)	76, 230, 988, 1015, 1882
Hpy99I	CGWCG	2 cut(s)	923, 1378
HpyAV	CCTTC	6 cut(s)	199, 682, 1614, 1709, 2084, 2435
HpyCH4III	ACNGT	9 cut(s)	329, 644, 947, 1063, 1337, 1378, 1681, 2110, 2189
HpyCH4IV	ACGT	2 cut(s)	921, 1995
HpyF10VI	GCNNNNNNNGC	7 cut(s)	419, 428, 569, 887, 936, 1096, 2146
HpyF3I	CTNAG	8 cut(s)	38, 149, 689, 747, 1578, 1800, 2022, 2120
HpySE526I	ACGT	2 cut(s)	921, 1995
Hsp92I	GRCGYC	3 cut(s)	539, 921, 2138
HspAI	GCGC	3 cut(s)	80, 399, 2138
KasI	GGCGCC	1 cut(s)	2137
KflI	GGGWCCC	1 cut(s)	283
Kpn2I	TCCGGA	1 cut(s)	443
Ksp22I	TGATCA	1 cut(s)	1540
Kzo9I	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
LguI	GCTCTTC	1 cut(s)	1080
LmnI	GCTCC	4 cut(s)	125, 878, 1634, 2099
Lsp1109I	GCAGC	6 cut(s)	412, 440, 989, 1058, 1083, 1677
LweI	GCATC	4 cut(s)	422, 572, 594, 1222
MaeI	CTAG	4 cut(s)	396, 558, 1583, 2354
MaeII	ACGT	2 cut(s)	921, 1995
MaeIII	GTNAC	7 cut(s)	209, 680, 998, 1153, 1184, 1378, 2129
MalI	GATC	6 cut(s)	62, 912, 1542, 1857, 2007, 2397
MbiI	CCGCTC	1 cut(s)	2102
MboI	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
MfeI	CAATTG	1 cut(s)	1207
MflI	RGATCY	2 cut(s)	910, 1855
MhlI	GDGCHC	1 cut(s)	122
MlsI	TGGCCA	1 cut(s)	2045
MluNI	TGGCCA	1 cut(s)	2045
Mly113I	GGCGCC	1 cut(s)	2138
MlyI	GAGTC	2 cut(s)	1181, 1408
MmeI	TCCRAC	2 cut(s)	1397, 2418
Mox20I	TGGCCA	1 cut(s)	2045
Mph1103I	ATGCAT	1 cut(s)	415
MroI	TCCGGA	1 cut(s)	443
MscI	TGGCCA	1 cut(s)	2045
MseI	TTAA	5 cut(s)	107, 240, 1689, 1775, 1866
MslI	CAYNNNNRTG	3 cut(s)	1310, 1444, 1481
Msp20I	TGGCCA	1 cut(s)	2045
MspA1I	CMGCKG	4 cut(s)	425, 585, 980, 2119
MspI	CCGG	4 cut(s)	116, 444, 864, 1077
MspR9I	CCNGG	7 cut(s)	116, 117, 518, 599, 1005, 1196, 2047
MunI	CAATTG	1 cut(s)	1207
Mva1269I	GAATGC	2 cut(s)	1439, 2161
MvaI	CCWGG	5 cut(s)	518, 599, 1005, 1196, 2047
MvnI	CGCG	2 cut(s)	80, 401
MwoI	GCNNNNNNNGC	7 cut(s)	419, 428, 569, 887, 936, 1096, 2146
NarI	GGCGCC	1 cut(s)	2138
NciI	CCSGG	2 cut(s)	116, 117
NcoI	CCATGG	1 cut(s)	543
NdeII	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
NlaIV	GGNNCC	8 cut(s)	121, 284, 285, 1432, 1651, 1934, 2055, 2139
NmuCI	GTSAC	2 cut(s)	1153, 1184
NsiI	ATGCAT	1 cut(s)	415
NspI	RCATGY	1 cut(s)	1486
PaeR7I	CTCGAG	2 cut(s)	276, 1465
PagI	TCATGA	1 cut(s)	2415
PciSI	GCTCTTC	1 cut(s)	1080
PctI	GAATGC	2 cut(s)	1439, 2161
PfeI	GAWTC	1 cut(s)	440
PkrI	GCNGC	8 cut(s)	424, 427, 430, 979, 1073, 1098, 1114, 1667
PleI	GAGTC	2 cut(s)	1181, 1407
PluTI	GGCGCC	1 cut(s)	2141
PpsI	GAGTC	2 cut(s)	1181, 1407
PpuMI	RGGWCCY	2 cut(s)	283, 1933
PsiI	TTATAA	1 cut(s)	1644
Psp5II	RGGWCCY	2 cut(s)	283, 1933
Psp6I	CCWGG	5 cut(s)	516, 597, 1003, 1194, 2045
PspGI	CCWGG	5 cut(s)	516, 597, 1003, 1194, 2045
PspN4I	GGNNCC	8 cut(s)	121, 284, 285, 1432, 1651, 1934, 2055, 2139
PspPI	GGNCC	2 cut(s)	283, 1933
PspPPI	RGGWCCY	2 cut(s)	283, 1933
PspXI	VCTCGAGB	1 cut(s)	276
PsrI	GAACNNNNNNTAC	2 cut(s)	1789, 1821
PstI	CTGCAG	1 cut(s)	430
PstNI	CAGNNNCTG	1 cut(s)	1235
PsuI	RGATCY	2 cut(s)	910, 1855
PvuII	CAGCTG	2 cut(s)	980, 2119
RseI	CAYNNNNRTG	3 cut(s)	1310, 1444, 1481
SapI	GCTCTTC	1 cut(s)	1080
SaqAI	TTAA	5 cut(s)	107, 240, 1689, 1775, 1866
SatI	GCNGC	8 cut(s)	423, 426, 429, 978, 1072, 1097, 1113, 1666
Sau3AI	GATC	6 cut(s)	60, 910, 1540, 1855, 2005, 2395
Sau96I	GGNCC	2 cut(s)	283, 1933
ScaI	AGTACT	2 cut(s)	43, 1550
SchI	GAGTC	2 cut(s)	1181, 1408
ScrFI	CCNGG	7 cut(s)	116, 117, 518, 599, 1005, 1196, 2047
SduI	GDGCHC	1 cut(s)	122
SfaNI	GCATC	4 cut(s)	422, 572, 594, 1222
SfcI	CTRYAG	3 cut(s)	426, 1062, 2185
SfoI	GGCGCC	1 cut(s)	2139
Sfr274I	CTCGAG	2 cut(s)	276, 1465
SinI	GGWCC	2 cut(s)	283, 1933
SlaI	CTCGAG	2 cut(s)	276, 1465
SmaI	CCCGGG	1 cut(s)	117
SmiMI	CAYNNNNRTG	3 cut(s)	1310, 1444, 1481
SmlI	CTYRAG	2 cut(s)	276, 1465
SmoI	CTYRAG	2 cut(s)	276, 1465
SrfI	GCCCGGGC	1 cut(s)	117
SsiI	CCGC	9 cut(s)	221, 423, 585, 635, 1099, 1112, 1182, 1853, 2102
SspDI	GGCGCC	1 cut(s)	2137
SspMI	CTAG	4 cut(s)	396, 558, 1583, 2354
StyD4I	CCNGG	7 cut(s)	114, 115, 516, 597, 1003, 1194, 2045
StyI	CCWWGG	2 cut(s)	543, 1116
TaaI	ACNGT	9 cut(s)	329, 644, 947, 1063, 1337, 1378, 1681, 2110, 2189
TaiI	ACGT	2 cut(s)	924, 1998
TaqI	TCGA	7 cut(s)	59, 277, 822, 1466, 1523, 2112, 2273
TaqII	GACCGA	1 cut(s)	330
TatI	WGTACW	5 cut(s)	19, 41, 840, 1548, 1796
TauI	GCSGC	2 cut(s)	425, 1115
TfiI	GAWTC	1 cut(s)	440
Tru1I	TTAA	5 cut(s)	107, 240, 1689, 1775, 1866
Tru9I	TTAA	5 cut(s)	107, 240, 1689, 1775, 1866
TscAI	CASTG	6 cut(s)	315, 739, 995, 1066, 1158, 1963
TseFI	GTSAC	2 cut(s)	1153, 1184
TseI	GCWGC	6 cut(s)	425, 428, 977, 1071, 1096, 1665
Tsp45I	GTSAC	2 cut(s)	1153, 1184
TspGWI	ACGGA	1 cut(s)	1648
TspMI	CCCGGG	1 cut(s)	115
TspRI	CASTG	6 cut(s)	315, 739, 995, 1066, 1158, 1963
VpaK11BI	GGWCC	2 cut(s)	283, 1933
XapI	RAATTY	3 cut(s)	268, 382, 1727
XceI	RCATGY	1 cut(s)	1486
XhoI	CTCGAG	2 cut(s)	276, 1465
XmaI	CCCGGG	1 cut(s)	115
XmiI	GTMKAC	1 cut(s)	229
XspI	CTAG	4 cut(s)	396, 558, 1583, 2354
ZraI	GACGTC	1 cut(s)	922
ZrmI	AGTACT	2 cut(s)	43, 1550
Zsp2I	ATGCAT	1 cut(s)	415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.