Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0003934

Physical Location & Seq

Genomic Coordinates Forward (+)

32876474 .. 32878242

Gene Structure 1769 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG275000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1769 bp

ATGGCTTCTTCTTCTGTACTGGTTATATTCTTACTCAGTACTGTTTTGATTATCTCGATCATGCCCAATGTAAAAGCTCAAACAAACCACTCCAAATCGAATTTAATCAGCCCGAACTCCTCTCTATCCCCCCATGTTAATAACCTCAATATTTCTTCATGGCTGTCATCTTCTGGCCGTTTCGCCTTCGGTTTCTACCCACAAGGCAACGGCTTTGCTGTTGGTATACAGCTTCTTAATCCACCACAAAACACAGTTGTGTGGACTGCAAATCGAGATGACCCCCCTGTCTCCCCAAACTCTACACTGGAACTAACAAGAGACGGTCTCTTGCTTCGGTCAGAGGGAGGCCAAGGAATACGTATTATTAATGGCTCAAGTTCACTAGAAACTAGTGCGATATCAAATGCATCAACTGCAGCTGCAGCTATGCTTGATTCCGGAAACTTTGTGCTGTATAATGAGAATTTTTTTGTTATATGGGAGAGCTTTGATTTCCCAACTGATACAATACTGGGAGGCCAAGATTTGCGGATGTCCATGGGTCTGGTATCTAGTGCATCTGTTGCAGACCACTCCAGCGGGCGATTTTCCCTGAAGATGCAGGACGATGGAAACCTTGTGGCCTACCCGCCAAACAGTTCTTCTGCTTATTGGGCTACTATGACCTATAGTAGTGGTTACCAACTTAGTCTCAACCTATCAGGCTTTCTCGTTTTGAATAGTGGTCTGAGTACTCAGGTCTTGGTAAACGGCTCGTACCATCACCCACAAGATAATCAAACCACAATCTACCGAGCAACACTTGATGTCGAAGGGAATTTCAGATTGTACTTGCATAACTTTCTGATGTCCGCTAGTTCAGAGCGAGCTGTAATGCCTATTTGGTCAGCTTTGCAACATCCATGCGACGTCCATGGCATTTGTGGCTTCAACAGTTATTGTGTAGTCATGCAAAAGAAAGCAGCTGAAGTTGAGTGCAAATGTTACCCTGGATTTGTTCACAACAACGCCAGTCAGAAGTCTCTGGGGTGCTTTCATAATTTCACTGTAGACGGCTGCACCGGAAATGAAGAGCCACGGCAGCGGTACAAGGTAGAGGCCTTGGACAATACATCGTGGAATAATCATCCTTATTCAGTGTCACCTATTCAAAACGAGGAACATTGCAGTGACTCTTGCATGAGAGACTGCAATTGTTGGGCTGTGCTATATACAGATGCTGATTGCAGAATATACAAGCTTCCACTTTTATATGGCAAAACAACTTCAAATATATCCTCTAAGGGTTTCTTCAAAGTGCTTCTACCGAGAAATATGGTTGGCATGCCAAACAATTTTCCCGAACCACCCATAAGCCGGACGGTAACTGAAAGCAAGAATAATAGTCTAATTCTAATTCTTGCCATCACTCTGGGTACCATTGCATTCCTGTGTTCTGTCCTTGCCATCTCGAGTTTCACCATATACAAGCACCGAGTTCATAGGTACGAGAAGCTGTTGAAACATTCGAGTTTGGCATTAGCTGATCAAGAGTACTTCACTTTACAATCGTTTTCTTATGCTGAGCTCGAAAGAGCAACAGATGGCTTCAAGGAAGAGATAGGAAGGGGTACTTTCGGAGCTGTTTATAAAGGAACTCTATGCGGGGGCAGCTCTAACAAAACTGTTGCTGTTAAAAGACTGGAGAAAGTGGTTGATGAAGGAGTAAGGGAATTTATAGCTGAAATCACCACCATTGGACGAACTCACCACAGAAACTTAATTCA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

589

Amino Acids

64.82

Weight (kDa)

6.62

Isoelectric Point (pI)

37.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	85 - 180	7.9e-16	D-mannose binding lectin
S_locus_glycop	PF00954	240 - 336	4.2e-07	S-locus glycoprotein domain
PK_Tyr_Ser-Thr	PF07714	531 - 589	3.1e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003934)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23390
prunus_persica	Prupe.6G165100_v2.0.a1 Prupe.6G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0118681 RchiOBHm_Chr2g0118741 RchiOBHm_Chr2g0118761
rosa_laevigata	RLG00000018419
rosa_multiflora	Rmu_co8322353.1_g000001 Rmu_sc0001814.1_g000026 Rmu_sc0001814.1_g000032 Rmu_sc0002700.1_g000009 Rmu_sc0002700.1_g000033 Rmu_sc0002700.1_g000035 Rmu_sc0002831.1_g000014 Rmu_sc0002831.1_g000017 Rmu_sc0002831.1_g000031 Rmu_sc0004399.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124760 Rroxscaffold_2G00124850 Rroxscaffold_2G00124890 Rroxscaffold_2G00125410 Rroxscaffold_2G00125420
rosa_rugosa	Rorug02G0218400
rosa_samantha	Rh2AG274700 Rh2AG275000 Rh2AG275300 Rh2BG286500 Rh2BG286700 Rh2BG287100 Rh2DG301500 Rh2DG301700
rosa_wichuraiana	Rw2G021850 Rw2G021890 Rw2G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1632
AasI	GACNNNNNNGTC	1 cut(s)	287
AatII	GACGTC	1 cut(s)	915
Acc65I	GGTACC	1 cut(s)	1418
AccB1I	GGYRCC	1 cut(s)	1418
AccI	GTMKAC	2 cut(s)	226, 1053
AccIII	TCCGGA	1 cut(s)	440
AciI	CCGC	6 cut(s)	532, 582, 632, 855, 1087, 1647
AcoI	YGGCCR	1 cut(s)	175
AcsI	RAATTY	4 cut(s)	100, 466, 820, 1715
AcuI	CTGAAG	2 cut(s)	617, 990
AcyI	GRCGYC	1 cut(s)	912
AfiI	CCNNNNNNNGG	1 cut(s)	1359
AgsI	TTSAA	7 cut(s)	721, 934, 1154, 1272, 1297, 1504, 1594
AhlI	ACTAGT	1 cut(s)	392
AjnI	CCWGG	1 cut(s)	991
AjuI	GAANNNNNNNTTGG	2 cut(s)	628, 660
AleI	CACNNNNGTG	1 cut(s)	257
Alw21I	GWGCWC	1 cut(s)	1572
Alw26I	GTCTC	6 cut(s)	295, 315, 332, 698, 1029, 1182
AlwNI	CAGNNNCTG	1 cut(s)	1223
Ama87I	CYCGRG	1 cut(s)	1453
Aor13HI	TCCGGA	1 cut(s)	440
AoxI	GGCC	5 cut(s)	175, 349, 520, 624, 1101
ApeKI	GCWGC	7 cut(s)	419, 422, 425, 965, 1059, 1084, 1653
ApoI	RAATTY	4 cut(s)	100, 466, 820, 1715
AseI	ATTAAT	1 cut(s)	369
Asp718I	GGTACC	1 cut(s)	1418
AsuHPI	GGTGA	5 cut(s)	758, 1137, 1453, 1723, 1742
AvaI	CYCGRG	1 cut(s)	1453
BanI	GGYRCC	1 cut(s)	1418
BanII	GRGCYC	1 cut(s)	1572
Bbv12I	GWGCWC	1 cut(s)	1572
BbvI	GCAGC	7 cut(s)	409, 431, 437, 977, 1046, 1096, 1665
BccI	CCATC	5 cut(s)	605, 771, 1415, 1457, 1580
BceAI	ACGGC	5 cut(s)	162, 226, 769, 1072, 1097
BciT130I	CCWGG	1 cut(s)	993
BclI	TGATCA	1 cut(s)	1528
BcoDI	GTCTC	6 cut(s)	295, 315, 332, 698, 1029, 1182
BcuI	ACTAGT	1 cut(s)	392
BfaI	CTAG	4 cut(s)	386, 393, 555, 858
BfmI	CTRYAG	4 cut(s)	417, 423, 670, 1050
BisI	GCNGC	7 cut(s)	420, 423, 426, 966, 1060, 1085, 1654
BlpI	GCTNAGC	1 cut(s)	1566
BlsI	GCNGC	7 cut(s)	421, 424, 427, 967, 1061, 1086, 1655
BmcAI	AGTACT	3 cut(s)	40, 736, 1538
Bme1390I	CCNGG	1 cut(s)	993
BmeT110I	CYCGRG	1 cut(s)	1453
BmiI	GGNNCC	1 cut(s)	1420
BmrFI	CCNGG	1 cut(s)	993
BmrI	ACTGGG	1 cut(s)	524
BmsI	GCATC	4 cut(s)	419, 569, 591, 1210
BmuI	ACTGGG	1 cut(s)	524
BplI	GAGNNNNNCTC	2 cut(s)	312, 344
BpmI	CTGGAG	2 cut(s)	562, 1706
Bpu1102I	GCTNAGC	1 cut(s)	1566
BpuEI	CTTGAG	1 cut(s)	361
BsaAI	YACGTR	1 cut(s)	362
BsaHI	GRCGYC	1 cut(s)	912
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	6 cut(s)	352, 540, 916, 991, 1079, 1104
BsaWI	WCCGGW	2 cut(s)	440, 1064
Bsc4I	CCNNNNNNNGG	1 cut(s)	1359
Bse1I	ACTGG	5 cut(s)	24, 312, 519, 1014, 1689
Bse3DI	GCAATG	2 cut(s)	1165, 1422
BseAI	TCCGGA	1 cut(s)	440
BseBI	CCWGG	1 cut(s)	993
BseDI	CCNNGG	6 cut(s)	352, 540, 916, 991, 1079, 1104
BseGI	GGATG	3 cut(s)	540, 901, 1129
BseLI	CCNNNNNNNGG	1 cut(s)	1359
BseMI	GCAATG	2 cut(s)	1165, 1422
BseMII	CTCAG	4 cut(s)	49, 722, 752, 1557
BseNI	ACTGG	5 cut(s)	24, 312, 519, 1014, 1689
BseRI	GAGGAG	1 cut(s)	109
BseXI	GCAGC	7 cut(s)	409, 431, 437, 977, 1046, 1096, 1665
BsgI	GTGCAG	1 cut(s)	1045
BshFI	GGCC	5 cut(s)	177, 351, 522, 626, 1103
BshNI	GGYRCC	1 cut(s)	1418
BsiHKAI	GWGCWC	1 cut(s)	1572
BsiHKCI	CYCGRG	1 cut(s)	1453
BsiSI	CCGG	3 cut(s)	441, 1065, 1360
BslI	CCNNNNNNNGG	1 cut(s)	1359
BsmAI	GTCTC	6 cut(s)	295, 315, 332, 698, 1029, 1182
BsmBI	CGTCTC	1 cut(s)	315
BsmI	GAATGC	1 cut(s)	1427
BsnI	GGCC	5 cut(s)	177, 351, 522, 626, 1103
Bso31I	GGTCTC	1 cut(s)	332
BsoBI	CYCGRG	1 cut(s)	1453
Bsp1286I	GDGCHC	1 cut(s)	1572
Bsp13I	TCCGGA	1 cut(s)	440
Bsp143I	GATC	2 cut(s)	57, 1528
Bsp1720I	GCTNAGC	1 cut(s)	1566
Bsp19I	CCATGG	2 cut(s)	540, 916
BspACI	CCGC	6 cut(s)	532, 582, 632, 855, 1087, 1647
BspANI	GGCC	5 cut(s)	177, 351, 522, 626, 1103
BspCNI	CTCAG	4 cut(s)	48, 723, 751, 1558
BspEI	TCCGGA	1 cut(s)	440
BspLI	GGNNCC	1 cut(s)	1420
BspMAI	CTGCAG	2 cut(s)	421, 427
BspQI	GCTCTTC	1 cut(s)	1068
BspT107I	GGYRCC	1 cut(s)	1418
BspTNI	GGTCTC	1 cut(s)	332
BsrDI	GCAATG	2 cut(s)	1165, 1422
BsrI	ACTGG	5 cut(s)	24, 312, 519, 1014, 1689
BssECI	CCNNGG	6 cut(s)	352, 540, 916, 991, 1079, 1104
BssMI	GATC	2 cut(s)	57, 1528
BssNAI	GTATAC	1 cut(s)	227
BssNI	GRCGYC	1 cut(s)	912
BssT1I	CCWWGG	4 cut(s)	352, 540, 916, 1104
Bst1107I	GTATAC	1 cut(s)	227
Bst2UI	CCWGG	1 cut(s)	993
Bst4CI	ACNGT	8 cut(s)	43, 256, 326, 641, 938, 1051, 1366, 1669
Bst6I	CTCTTC	2 cut(s)	1068, 1593
BstACI	GRCGYC	1 cut(s)	912
BstAPI	GCANNNNNTGC	2 cut(s)	416, 566
BstBAI	YACGTR	1 cut(s)	362
BstC8I	GCNNGC	3 cut(s)	584, 870, 1328
BstDEI	CTNAG	6 cut(s)	35, 689, 731, 738, 1284, 1566
BstDSI	CCRYGG	3 cut(s)	540, 916, 1079
BstEII	GGTNACC	1 cut(s)	680
BstF5I	GGATG	3 cut(s)	540, 901, 1129
BstKTI	GATC	2 cut(s)	60, 1531
BstMAI	GTCTC	6 cut(s)	295, 315, 332, 698, 1029, 1182
BstMBI	GATC	2 cut(s)	57, 1528
BstMWI	GCNNNNNNNGC	7 cut(s)	416, 425, 566, 656, 927, 1084, 1653
BstNI	CCWGG	1 cut(s)	993
BstNSI	RCATGY	1 cut(s)	1330
BstPI	GGTNACC	1 cut(s)	680
BstSCI	CCNGG	1 cut(s)	991
BstSFI	CTRYAG	4 cut(s)	417, 423, 670, 1050
BstSNI	TACGTA	1 cut(s)	362
BstV1I	GCAGC	7 cut(s)	409, 431, 437, 977, 1046, 1096, 1665
BstXI	CCANNNNNNTGG	2 cut(s)	547, 1414
BstZ17I	GTATAC	1 cut(s)	227
BsuRI	GGCC	5 cut(s)	177, 351, 522, 626, 1103
BtgI	CCRYGG	3 cut(s)	540, 916, 1079
BtsCI	GGATG	3 cut(s)	540, 901, 1129
BtsI	GCAGTG	1 cut(s)	1177
BtsIMutI	CAGTG	4 cut(s)	305, 1047, 1146, 1177
Cac8I	GCNNGC	3 cut(s)	584, 870, 1328
CaiI	CAGNNNCTG	1 cut(s)	1223
CviAII	CATG	9 cut(s)	61, 134, 159, 541, 906, 917, 952, 1183, 1327
DdeI	CTNAG	6 cut(s)	35, 689, 731, 738, 1284, 1566
DpnI	GATC	2 cut(s)	59, 1530
DpnII	GATC	2 cut(s)	57, 1528
DrdI	GACNNNNNNGTC	1 cut(s)	287
DseDI	GACNNNNNNGTC	1 cut(s)	287
EaeI	YGGCCR	1 cut(s)	175
Eam1104I	CTCTTC	2 cut(s)	1068, 1593
EarI	CTCTTC	2 cut(s)	1068, 1593
Ecl136II	GAGCTC	1 cut(s)	1570
Eco105I	TACGTA	1 cut(s)	362
Eco130I	CCWWGG	4 cut(s)	352, 540, 916, 1104
Eco147I	AGGCCT	1 cut(s)	1103
Eco24I	GRGCYC	1 cut(s)	1572
Eco31I	GGTCTC	1 cut(s)	332
Eco32I	GATATC	1 cut(s)	402
Eco53kI	GAGCTC	1 cut(s)	1570
Eco57I	CTGAAG	2 cut(s)	617, 990
Eco88I	CYCGRG	1 cut(s)	1453
Eco91I	GGTNACC	1 cut(s)	680
EcoICRI	GAGCTC	1 cut(s)	1570
EcoO65I	GGTNACC	1 cut(s)	680
EcoRII	CCWGG	1 cut(s)	991
EcoRV	GATATC	1 cut(s)	402
EcoT14I	CCWWGG	4 cut(s)	352, 540, 916, 1104
EcoT22I	ATGCAT	1 cut(s)	412
EcoT38I	GRGCYC	1 cut(s)	1572
ErhI	CCWWGG	4 cut(s)	352, 540, 916, 1104
Esp3I	CGTCTC	1 cut(s)	315
FaeI	CATG	9 cut(s)	64, 137, 162, 544, 909, 920, 955, 1186, 1330
FalI	AAGNNNNNCTT	4 cut(s)	1277, 1309, 1600, 1632
FatI	CATG	9 cut(s)	60, 133, 158, 540, 905, 916, 951, 1182, 1326
FauI	CCCGC	3 cut(s)	575, 639, 1640
FbaI	TGATCA	1 cut(s)	1528
FblI	GTMKAC	2 cut(s)	226, 1053
Fnu4HI	GCNGC	7 cut(s)	420, 423, 426, 966, 1060, 1085, 1654
FokI	GGATG	3 cut(s)	547, 888, 1116
FriOI	GRGCYC	1 cut(s)	1572
Fsp4HI	GCNGC	7 cut(s)	420, 423, 426, 966, 1060, 1085, 1654
FspBI	CTAG	4 cut(s)	386, 393, 555, 858
GluI	GCNGC	7 cut(s)	420, 423, 426, 966, 1060, 1085, 1654
GsuI	CTGGAG	2 cut(s)	562, 1706
HaeIII	GGCC	5 cut(s)	177, 351, 522, 626, 1103
HapII	CCGG	3 cut(s)	441, 1065, 1360
Hin1I	GRCGYC	1 cut(s)	912
Hin1II	CATG	9 cut(s)	64, 137, 162, 544, 909, 920, 955, 1186, 1330
HindIII	AAGCTT	1 cut(s)	1241
HinfI	GANTC	2 cut(s)	437, 1175
HpaII	CCGG	3 cut(s)	441, 1065, 1360
HphI	GGTGA	5 cut(s)	758, 1137, 1453, 1723, 1742
Hpy166II	GTNNAC	6 cut(s)	227, 264, 383, 751, 1003, 1054
Hpy188I	TCNGA	7 cut(s)	343, 732, 827, 849, 865, 1020, 1622
Hpy188III	TCNNGA	6 cut(s)	55, 275, 441, 1343, 1453, 1532
Hpy8I	GTNNAC	6 cut(s)	227, 264, 383, 751, 1003, 1054
Hpy99I	CGWCG	1 cut(s)	914
HpyAV	CCTTC	4 cut(s)	196, 809, 1602, 1697
HpyCH4III	ACNGT	8 cut(s)	43, 256, 326, 641, 938, 1051, 1366, 1669
HpyCH4IV	ACGT	2 cut(s)	361, 912
HpyF10VI	GCNNNNNNNGC	7 cut(s)	416, 425, 566, 656, 927, 1084, 1653
HpyF3I	CTNAG	6 cut(s)	35, 689, 731, 738, 1284, 1566
HpySE526I	ACGT	2 cut(s)	361, 912
Hsp92I	GRCGYC	1 cut(s)	912
Hsp92II	CATG	9 cut(s)	64, 137, 162, 544, 909, 920, 955, 1186, 1330
Kpn2I	TCCGGA	1 cut(s)	440
KpnI	GGTACC	1 cut(s)	1422
Ksp22I	TGATCA	1 cut(s)	1528
Kzo9I	GATC	2 cut(s)	57, 1528
LguI	GCTCTTC	1 cut(s)	1068
LmnI	GCTCC	1 cut(s)	1622
Lsp1109I	GCAGC	7 cut(s)	409, 431, 437, 977, 1046, 1096, 1665
LweI	GCATC	4 cut(s)	419, 569, 591, 1210
MaeI	CTAG	4 cut(s)	386, 393, 555, 858
MaeII	ACGT	2 cut(s)	361, 912
MaeIII	GTNAC	5 cut(s)	680, 986, 1143, 1172, 1366
MalI	GATC	2 cut(s)	59, 1530
MboI	GATC	2 cut(s)	57, 1528
MboII	GAAGA	8 cut(s)	3, 147, 162, 610, 636, 1085, 1285, 1610
MfeI	CAATTG	1 cut(s)	1195
MhlI	GDGCHC	1 cut(s)	1572
MlyI	GAGTC	1 cut(s)	1169
MnlI	CCTC	8 cut(s)	130, 155, 337, 341, 512, 1093, 1153, 1291
Mph1103I	ATGCAT	1 cut(s)	412
MroI	TCCGGA	1 cut(s)	440
MseI	TTAA	6 cut(s)	104, 138, 237, 369, 1677, 1763
MslI	CAYNNNNRTG	3 cut(s)	257, 1170, 1432
MspA1I	CMGCKG	4 cut(s)	422, 582, 968, 1087
MspI	CCGG	3 cut(s)	441, 1065, 1360
MspR9I	CCNGG	1 cut(s)	993
MunI	CAATTG	1 cut(s)	1195
Mva1269I	GAATGC	1 cut(s)	1427
MvaI	CCWGG	1 cut(s)	993
MwoI	GCNNNNNNNGC	7 cut(s)	416, 425, 566, 656, 927, 1084, 1653
NcoI	CCATGG	2 cut(s)	540, 916
NdeII	GATC	2 cut(s)	57, 1528
NlaIII	CATG	9 cut(s)	64, 137, 162, 544, 909, 920, 955, 1186, 1330
NlaIV	GGNNCC	1 cut(s)	1420
NmuCI	GTSAC	2 cut(s)	1143, 1172
NsiI	ATGCAT	1 cut(s)	412
NspI	RCATGY	1 cut(s)	1330
OliI	CACNNNNGTG	1 cut(s)	257
PaeI	GCATGC	1 cut(s)	1330
PaeR7I	CTCGAG	1 cut(s)	1453
PceI	AGGCCT	1 cut(s)	1103
PciSI	GCTCTTC	1 cut(s)	1068
PctI	GAATGC	1 cut(s)	1427
PfeI	GAWTC	1 cut(s)	437
PkrI	GCNGC	7 cut(s)	421, 424, 427, 967, 1061, 1086, 1655
PleI	GAGTC	1 cut(s)	1169
PpsI	GAGTC	1 cut(s)	1169
Ppu21I	YACGTR	1 cut(s)	362
PshBI	ATTAAT	1 cut(s)	369
PsiI	TTATAA	1 cut(s)	1632
Psp124BI	GAGCTC	1 cut(s)	1572
Psp6I	CCWGG	1 cut(s)	991
PspEI	GGTNACC	1 cut(s)	680
PspGI	CCWGG	1 cut(s)	991
PspN4I	GGNNCC	1 cut(s)	1420
PstI	CTGCAG	2 cut(s)	421, 427
PstNI	CAGNNNCTG	1 cut(s)	1223
PvuII	CAGCTG	2 cut(s)	422, 968
RseI	CAYNNNNRTG	3 cut(s)	257, 1170, 1432
SacI	GAGCTC	1 cut(s)	1572
SapI	GCTCTTC	1 cut(s)	1068
SaqAI	TTAA	6 cut(s)	104, 138, 237, 369, 1677, 1763
SatI	GCNGC	7 cut(s)	420, 423, 426, 966, 1060, 1085, 1654
Sau3AI	GATC	2 cut(s)	57, 1528
ScaI	AGTACT	3 cut(s)	40, 736, 1538
SchI	GAGTC	1 cut(s)	1169
ScrFI	CCNGG	1 cut(s)	993
SduI	GDGCHC	1 cut(s)	1572
SfaNI	GCATC	4 cut(s)	419, 569, 591, 1210
SfcI	CTRYAG	4 cut(s)	417, 423, 670, 1050
Sfr274I	CTCGAG	1 cut(s)	1453
SlaI	CTCGAG	1 cut(s)	1453
SmiMI	CAYNNNNRTG	3 cut(s)	257, 1170, 1432
SmlI	CTYRAG	2 cut(s)	376, 1453
SmoI	CTYRAG	2 cut(s)	376, 1453
SnaBI	TACGTA	1 cut(s)	362
SpeI	ACTAGT	1 cut(s)	392
SphI	GCATGC	1 cut(s)	1330
SseBI	AGGCCT	1 cut(s)	1103
SsiI	CCGC	6 cut(s)	532, 582, 632, 855, 1087, 1647
SspI	AATATT	1 cut(s)	151
SspMI	CTAG	4 cut(s)	386, 393, 555, 858
SstI	GAGCTC	1 cut(s)	1572
StuI	AGGCCT	1 cut(s)	1103
StyD4I	CCNGG	1 cut(s)	991
StyI	CCWWGG	4 cut(s)	352, 540, 916, 1104
TaaI	ACNGT	8 cut(s)	43, 256, 326, 641, 938, 1051, 1366, 1669
TaiI	ACGT	2 cut(s)	364, 915
TaqI	TCGA	7 cut(s)	56, 98, 274, 813, 1454, 1511, 1572
TaqII	GACCGA	1 cut(s)	327
TatI	WGTACW	5 cut(s)	16, 38, 734, 831, 1536
TfiI	GAWTC	1 cut(s)	437
Tru1I	TTAA	6 cut(s)	104, 138, 237, 369, 1677, 1763
Tru9I	TTAA	6 cut(s)	104, 138, 237, 369, 1677, 1763
TscAI	CASTG	4 cut(s)	312, 1054, 1146, 1177
TseFI	GTSAC	2 cut(s)	1143, 1172
TseI	GCWGC	7 cut(s)	419, 422, 425, 965, 1059, 1084, 1653
Tsp45I	GTSAC	2 cut(s)	1143, 1172
TspDTI	ATGAA	5 cut(s)	147, 1028, 1086, 1472, 1716
TspRI	CASTG	4 cut(s)	312, 1054, 1146, 1177
VspI	ATTAAT	1 cut(s)	369
XapI	RAATTY	4 cut(s)	100, 466, 820, 1715
XceI	RCATGY	1 cut(s)	1330
XcmI	CCANNNNNNNNNTGG	1 cut(s)	923
XhoI	CTCGAG	1 cut(s)	1453
XmiI	GTMKAC	2 cut(s)	226, 1053
XspI	CTAG	4 cut(s)	386, 393, 555, 858
ZraI	GACGTC	1 cut(s)	913
ZrmI	AGTACT	3 cut(s)	40, 736, 1538
Zsp2I	ATGCAT	1 cut(s)	412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.