Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003934

Physical Location & Seq

Genomic Coordinates Reverse (-)

30530475 .. 30533282

Gene Structure 2808 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G021890.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGGCTTCCTCTTCTGTACTGGTTATATTCTTACTCAGTACTGTTTTGATTATCTCGATCATGCCCAATGTAAAAGCTCAAAGAAACCACTCCAAATCCAATTTAATCAGCCTGGGCTCCTCTCTATCACCCCATGTTAATAACCTCAGTATTTCTTCATGGCTGTCATCTTCTGGCCGTTTCGCCTTCGGTTTCTACCCACAAGGAAACGGCTTTGCTGTTGGTATACAGCTTCTTAATCCACCACAAAACACAGTTGTGTGGACTGCAAATCGAGATGACCCCCCTGTCTCCCCAAACTCTACACTGAAACTAACAAGAGACGGTATCTTGCTTCAGTCAGAGGGAGGCCAAGAAATACGTATTACTAATGGCTTAAGTTTACTGGAAACTAGTGCGATATCAAATGCATCAACTGCAGCTGCAGCTATGCTTGATTCCGGAAACTTTGTGCTGTATAATGAGACGTTTTTTGTCATATGGAAGAGCTTTCTTTCCCCAACTGATACAATACTGGCAGGACAAGATTTGCAGATGTCCATGGGTCTGGTATCTAGTGCATCTGTCGCAGACCACTCCAGCGGGCGATTTTCCCTGGAGATGCGGAATGATGGAAACCTTGCAGCCTACCCGCCAAACAGTTCTTCTGCTTATTGGTCTGTTATGATGACCACTAGTTACCCTATGATGACCACTAGTTACCGACTTAGTCTCAACCTATCAGGCTTTCTCGTTTTGAATAGCCAAAGAGGCCAATACATCAGTCTGAGTACTCCGACCACTCAGGTCTTGGCAAACAGATCGGACCATCCGCCACAAGACAATCAAACCACAATCTACCGAGCAACACTTGATGTCGATGGGAATTTCAGATTGTACTTGCATAACTTTCCGATGTCCGGTAGTTCAGAGGGAGCTGTAATGGCTATTTGGTCAGCTTTGCAACATCCATGCGACGTCCAAGGCATTTGTGGCTTCAACAGTTATTGTGAAGTAGTCATGCAAAAGAAAGCAGCTGAAGTTGAGTGCAAATGTTACCCTGGATTTGTTTACAACAACGCCAGTCAGAAGTCTCTGGGGTGCTATCATAATTTCAGTGTAGAGGGCTGCACCGGAAATGAAGAGCCACGGCTGCGGTACAAGGTCGAGGCCTTGGACAATACATCGTGGAATAATCATCCTTATTTAGTGACACCTATTAGAAACAAGGAAGGTTGCAGCGATTCTTGCCTGGGAGACTGCAATTGTTGGGCTGTGCTATATACAGATGGTAACTGCAGAAAATACAAGCTTCCACTTTTATATGGCAAAAAAAATTCAAGTATATCCTCTAAAGGTTTCATCAAAGTGATTCTATCGGGAAACATGGACAGTATGCCAAATAATTTTCCCGACCTACGCAAAAGTCCGACGGTAGCTGAAAGCAAGAATAATAGTCTAATTCTAATTCTTGCTATCAGTTTGGGTTCCATTGCATTCTTGTGTTCTGTCTTTGCCATCTCGAGTTTCACCATATACAAGCACCGAGTTCATAGGTACGAGAAGCTCTTGAAACAATGGAGTTTGGCATTAGCTGATCAAGAGTACTTCACTTTACAATCGTTTTCTTATGCTGAGCTCGAAAGAGCAACAGATGGCTTCAAGGAAGAGATAGGAAGGGGTACTTTCGGAGCTGTTTATAAAGGAACTCTATCCGGGGGCAGCTCTAACAAAACTGTTGCTGTTAAAAGACTGGAGAAAGTGGTTGATGAAGGAGTAAGGGAATTTATAGCTGAAATTACCACCATTGGACGAACTCACCACAGAAACTTAATTCAGTTGCTTGGTTTCTGTATTGAGGGTTCTCGGAAGCTTCTTGTTTATGAATACATGAGCAACGGCTCACTTGCGGATCTTATCTTTAAGGCCAAAAGTGTGCACTCATCTTGGAAAGAAAGGGTAAGACTTGTATTCGATGTGGCTAAAGGGGTCCTCTATTTACATGACGAATGCAGTGTCCATATCATTCATTCCAATTTGAAACCCCAAAACGTACTCTTGGATCATACTTGGACGGCTAAGATATCTGATTTCGGTTTGGCCAGGCTGGTGCCAAATGAAACAAAAATCAACTCTACTGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGCGGAAACAGTCGAACAGGTGAGAAGAAGTTACTTGGCGCCTGAATGGCAGAAGAATGCTCTCATATCTGTAAAAGCTGATATCTACAGTTTTGGTATTGTGCTTTTAGAGATTGTATGTTGTAGAAGAAACATAGAAGTAAATGTTTCAACGCCAGATGAGATTATTCTTTCGAGTTGGGTCAGTAAATGCTTTGCTGCTGGACAATTAGATAAGCTTGTGGAGGAAGATGAAAATGTAGATTTGAAGACCCTAGAAAGAATGGTGAAAGTGGGGTTGTGGTGCGTTCAAGATGATCCAGCTTTGCGTCCTTTCATGAAGAATGTAATCTTGATGTTGGAAGGGACAATGGACATACCAGTTCTACCAAATCCAGAGCTTCCCTCTCTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

847

Amino Acids

93.26

Weight (kDa)

6.97

Isoelectric Point (pI)

36.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	85 - 180	8.2e-15	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	547 - 825	1.8e-42	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	548 - 824	1.3e-39	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003934)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23390
prunus_persica	Prupe.6G165100_v2.0.a1 Prupe.6G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0118681 RchiOBHm_Chr2g0118741 RchiOBHm_Chr2g0118761
rosa_laevigata	RLG00000018419
rosa_multiflora	Rmu_co8322353.1_g000001 Rmu_sc0001814.1_g000026 Rmu_sc0001814.1_g000032 Rmu_sc0002700.1_g000009 Rmu_sc0002700.1_g000033 Rmu_sc0002700.1_g000035 Rmu_sc0002831.1_g000014 Rmu_sc0002831.1_g000017 Rmu_sc0002831.1_g000031 Rmu_sc0004399.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124760 Rroxscaffold_2G00124850 Rroxscaffold_2G00124890 Rroxscaffold_2G00125410 Rroxscaffold_2G00125420
rosa_rugosa	Rorug02G0218400
rosa_samantha	Rh2AG274700 Rh2AG275000 Rh2AG275300 Rh2BG286500 Rh2BG286700 Rh2BG287100 Rh2DG301500 Rh2DG301700
rosa_wichuraiana	Rw2G021850 Rw2G021890 Rw2G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1680
AasI	GACNNNNNNGTC	1 cut(s)	287
AatII	GACGTC	1 cut(s)	960
AccB1I	GGYRCC	2 cut(s)	2089, 2185
AccBSI	CCGCTC	1 cut(s)	2150
AccI	GTMKAC	1 cut(s)	226
AccIII	TCCGGA	1 cut(s)	440
AciI	CCGC	7 cut(s)	582, 604, 632, 812, 1135, 1889, 2150
AclWI	GGATC	3 cut(s)	1899, 2049, 2438
AcoI	YGGCCR	2 cut(s)	175, 2079
AcsI	RAATTY	3 cut(s)	865, 1315, 1763
AcuI	CTGAAG	2 cut(s)	320, 1038
AcyI	GRCGYC	2 cut(s)	957, 2186
AfaI	GTAC	9 cut(s)	18, 40, 772, 878, 1139, 1538, 1586, 1663, 2034
AfiI	CCNNNNNNNGG	1 cut(s)	899
AflII	CTTAAG	1 cut(s)	376
AgsI	TTSAA	9 cut(s)	739, 979, 1320, 1552, 1642, 2020, 2298, 2395, 2438
AhlI	ACTAGT	3 cut(s)	392, 674, 695
AjnI	CCWGG	5 cut(s)	111, 594, 1039, 1230, 2081
AjuI	GAANNNNNNNTTGG	2 cut(s)	628, 660
AleI	CACNNNNGTG	1 cut(s)	257
Alw21I	GWGCWC	2 cut(s)	1620, 1920
Alw26I	GTCTC	6 cut(s)	295, 315, 458, 716, 1077, 1230
Alw44I	GTGCAC	1 cut(s)	1916
AlwI	GGATC	3 cut(s)	1899, 2049, 2438
Ama87I	CYCGRG	1 cut(s)	1501
Aor13HI	TCCGGA	1 cut(s)	440
AoxI	GGCC	6 cut(s)	175, 349, 751, 1149, 1905, 2079
ApaLI	GTGCAC	1 cut(s)	1916
ApoI	RAATTY	3 cut(s)	865, 1315, 1763
ArsI	GACNNNNNNTTYG	2 cut(s)	1980, 2012
AspLEI	GCGC	1 cut(s)	2188
AspS9I	GGNCC	2 cut(s)	805, 1969
AsuC2I	CCSGG	1 cut(s)	1696
AsuHPI	GGTGA	5 cut(s)	120, 1501, 1790, 2179, 2425
AvaI	CYCGRG	1 cut(s)	1501
AvaII	GGWCC	2 cut(s)	805, 1969
BaeGI	GKGCMC	1 cut(s)	1920
BalI	TGGCCA	1 cut(s)	2081
BanI	GGYRCC	2 cut(s)	2089, 2185
BanII	GRGCYC	2 cut(s)	119, 1620
BbsI	GAAGAC	1 cut(s)	2402
Bbv12I	GWGCWC	2 cut(s)	1620, 1920
BccI	CCATC	6 cut(s)	605, 816, 854, 1262, 1505, 1628
BceAI	ACGGC	5 cut(s)	162, 226, 1145, 1894, 2070
BcgI	CGANNNNNNTGC	2 cut(s)	783, 817
BciT130I	CCWGG	5 cut(s)	113, 596, 1041, 1232, 2083
BclI	TGATCA	1 cut(s)	1576
BcnI	CCSGG	1 cut(s)	1696
BcoDI	GTCTC	6 cut(s)	295, 315, 458, 716, 1077, 1230
BcuI	ACTAGT	3 cut(s)	392, 674, 695
BfaI	CTAG	5 cut(s)	393, 555, 675, 696, 2402
BfmI	CTRYAG	4 cut(s)	417, 423, 1276, 2233
BfoI	RGCGCY	1 cut(s)	2189
BfrI	CTTAAG	1 cut(s)	376
BglI	GCCNNNNNGGC	2 cut(s)	750, 2194
BlpI	GCTNAGC	1 cut(s)	1614
BmcAI	AGTACT	3 cut(s)	40, 772, 1586
Bme1390I	CCNGG	6 cut(s)	113, 596, 1041, 1232, 1696, 2083
Bme18I	GGWCC	2 cut(s)	805, 1969
BmeT110I	CYCGRG	1 cut(s)	1501
BmgT120I	GGNCC	2 cut(s)	805, 1969
BmiI	GGNNCC	5 cut(s)	118, 1468, 1970, 2091, 2187
BmrFI	CCNGG	6 cut(s)	113, 596, 1041, 1232, 1696, 2083
BmsI	GCATC	3 cut(s)	419, 569, 591
BpiI	GAAGAC	1 cut(s)	2402
BplI	GAGNNNNNCTC	1 cut(s)	2516
BpmI	CTGGAG	4 cut(s)	562, 617, 1754, 2139
Bpu1102I	GCTNAGC	1 cut(s)	1614
BpuMI	CCSGG	1 cut(s)	1696
BsaAI	YACGTR	1 cut(s)	362
BsaHI	GRCGYC	2 cut(s)	957, 2186
BsaJI	CCNNGG	9 cut(s)	112, 540, 594, 961, 1039, 1127, 1152, 1231, 1695
BsaWI	WCCGGW	3 cut(s)	440, 899, 1112
Bsc4I	CCNNNNNNNGG	1 cut(s)	899
Bse1I	ACTGG	7 cut(s)	24, 390, 519, 1062, 1737, 2122, 2507
Bse3DI	GCAATG	1 cut(s)	1470
BseAI	TCCGGA	1 cut(s)	440
BseBI	CCWGG	5 cut(s)	113, 596, 1041, 1232, 2083
BseDI	CCNNGG	9 cut(s)	112, 540, 594, 961, 1039, 1127, 1152, 1231, 1695
BseGI	GGATG	3 cut(s)	808, 946, 1177
BseLI	CCNNNNNNNGG	1 cut(s)	899
BseMI	GCAATG	1 cut(s)	1470
BseMII	CTCAG	5 cut(s)	49, 160, 758, 797, 1605
BseNI	ACTGG	7 cut(s)	24, 390, 519, 1062, 1737, 2122, 2507
BseSI	GKGCMC	1 cut(s)	1920
BsgI	GTGCAG	1 cut(s)	1093
BshFI	GGCC	6 cut(s)	177, 351, 753, 1151, 1907, 2081
BshNI	GGYRCC	2 cut(s)	2089, 2185
BsiHKAI	GWGCWC	2 cut(s)	1620, 1920
BsiHKCI	CYCGRG	1 cut(s)	1501
BsiSI	CCGG	4 cut(s)	441, 900, 1113, 1695
BslFI	GGGAC	1 cut(s)	2506
BslI	CCNNNNNNNGG	1 cut(s)	899
BsmAI	GTCTC	6 cut(s)	295, 315, 458, 716, 1077, 1230
BsmBI	CGTCTC	2 cut(s)	315, 458
BsmFI	GGGAC	1 cut(s)	2506
BsmI	GAATGC	3 cut(s)	1475, 1994, 2209
BsnI	GGCC	6 cut(s)	177, 351, 753, 1151, 1907, 2081
BsoBI	CYCGRG	1 cut(s)	1501
Bsp1286I	GDGCHC	3 cut(s)	119, 1620, 1920
Bsp13I	TCCGGA	1 cut(s)	440
Bsp143I	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
Bsp1720I	GCTNAGC	1 cut(s)	1614
Bsp19I	CCATGG	1 cut(s)	540
BspACI	CCGC	7 cut(s)	582, 604, 632, 812, 1135, 1889, 2150
BspANI	GGCC	6 cut(s)	177, 351, 753, 1151, 1907, 2081
BspCNI	CTCAG	5 cut(s)	48, 159, 759, 796, 1606
BspEI	TCCGGA	1 cut(s)	440
BspHI	TCATGA	1 cut(s)	2463
BspLI	GGNNCC	5 cut(s)	118, 1468, 1970, 2091, 2187
BspMAI	CTGCAG	3 cut(s)	421, 427, 1280
BspPI	GGATC	3 cut(s)	1899, 2049, 2438
BspQI	GCTCTTC	2 cut(s)	479, 1116
BspT107I	GGYRCC	2 cut(s)	2089, 2185
BspTI	CTTAAG	1 cut(s)	376
BsrBI	CCGCTC	1 cut(s)	2150
BsrDI	GCAATG	1 cut(s)	1470
BsrI	ACTGG	7 cut(s)	24, 390, 519, 1062, 1737, 2122, 2507
BssECI	CCNNGG	9 cut(s)	112, 540, 594, 961, 1039, 1127, 1152, 1231, 1695
BssMI	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
BssNAI	GTATAC	1 cut(s)	227
BssNI	GRCGYC	2 cut(s)	957, 2186
BssT1I	CCWWGG	3 cut(s)	540, 961, 1152
Bst1107I	GTATAC	1 cut(s)	227
Bst2UI	CCWGG	5 cut(s)	113, 596, 1041, 1232, 2083
Bst6I	CTCTTC	4 cut(s)	16, 479, 1116, 1641
BstACI	GRCGYC	2 cut(s)	957, 2186
BstAFI	CTTAAG	1 cut(s)	376
BstAPI	GCANNNNNTGC	1 cut(s)	416
BstBAI	YACGTR	1 cut(s)	362
BstC8I	GCNNGC	1 cut(s)	584
BstDEI	CTNAG	7 cut(s)	35, 146, 707, 767, 783, 1614, 2058
BstDSI	CCRYGG	2 cut(s)	540, 1127
BstF5I	GGATG	3 cut(s)	808, 946, 1177
BstH2I	RGCGCY	1 cut(s)	2189
BstHHI	GCGC	1 cut(s)	2188
BstKTI	GATC	6 cut(s)	60, 803, 1579, 1894, 2044, 2446
BstMAI	GTCTC	6 cut(s)	295, 315, 458, 716, 1077, 1230
BstMBI	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
BstMWI	GCNNNNNNNGC	9 cut(s)	416, 425, 566, 750, 923, 972, 1132, 1227, 2194
BstNI	CCWGG	5 cut(s)	113, 596, 1041, 1232, 2083
BstSCI	CCNGG	6 cut(s)	111, 594, 1039, 1230, 1694, 2081
BstSFI	CTRYAG	4 cut(s)	417, 423, 1276, 2233
BstSLI	GKGCMC	1 cut(s)	1920
BstSNI	TACGTA	1 cut(s)	362
BstV2I	GAAGAC	1 cut(s)	2402
BstX2I	RGATCY	1 cut(s)	1891
BstXI	CCANNNNNNTGG	1 cut(s)	547
BstYI	RGATCY	1 cut(s)	1891
BstZ17I	GTATAC	1 cut(s)	227
BsuRI	GGCC	6 cut(s)	177, 351, 753, 1151, 1907, 2081
BtgI	CCRYGG	2 cut(s)	540, 1127
BtsCI	GGATG	3 cut(s)	808, 946, 1177
BtsI	GCAGTG	1 cut(s)	1999
BtsIMutI	CAGTG	3 cut(s)	305, 1102, 1999
Cac8I	GCNNGC	1 cut(s)	584
CciI	TCATGA	1 cut(s)	2463
CfoI	GCGC	1 cut(s)	2188
Cfr13I	GGNCC	2 cut(s)	805, 1969
CseI	GACGC	1 cut(s)	2444
Csp6I	GTAC	9 cut(s)	17, 39, 771, 877, 1138, 1537, 1585, 1662, 2033
CviQI	GTAC	9 cut(s)	17, 39, 771, 877, 1138, 1537, 1585, 1662, 2033
DdeI	CTNAG	7 cut(s)	35, 146, 707, 767, 783, 1614, 2058
DinI	GGCGCC	1 cut(s)	2187
DpnI	GATC	6 cut(s)	59, 802, 1578, 1893, 2043, 2445
DpnII	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
DrdI	GACNNNNNNGTC	1 cut(s)	287
DseDI	GACNNNNNNGTC	1 cut(s)	287
EaeI	YGGCCR	2 cut(s)	175, 2079
Eam1104I	CTCTTC	4 cut(s)	16, 479, 1116, 1641
EarI	CTCTTC	4 cut(s)	16, 479, 1116, 1641
EciI	GGCGGA	1 cut(s)	801
Ecl136II	GAGCTC	1 cut(s)	1618
Eco105I	TACGTA	1 cut(s)	362
Eco130I	CCWWGG	3 cut(s)	540, 961, 1152
Eco147I	AGGCCT	1 cut(s)	1151
Eco24I	GRGCYC	2 cut(s)	119, 1620
Eco32I	GATATC	3 cut(s)	402, 2064, 2230
Eco47I	GGWCC	2 cut(s)	805, 1969
Eco53kI	GAGCTC	1 cut(s)	1618
Eco57I	CTGAAG	2 cut(s)	320, 1038
Eco88I	CYCGRG	1 cut(s)	1501
EcoICRI	GAGCTC	1 cut(s)	1618
EcoO109I	RGGNCCY	1 cut(s)	1969
EcoRII	CCWGG	5 cut(s)	111, 594, 1039, 1230, 2081
EcoRV	GATATC	3 cut(s)	402, 2064, 2230
EcoT14I	CCWWGG	3 cut(s)	540, 961, 1152
EcoT22I	ATGCAT	1 cut(s)	412
EcoT38I	GRGCYC	2 cut(s)	119, 1620
EgeI	GGCGCC	1 cut(s)	2187
EheI	GGCGCC	1 cut(s)	2187
ErhI	CCWWGG	3 cut(s)	540, 961, 1152
Esp3I	CGTCTC	2 cut(s)	315, 458
FalI	AAGNNNNNCTT	2 cut(s)	1648, 1680
FaqI	GGGAC	1 cut(s)	2506
FauI	CCCGC	2 cut(s)	575, 639
FauNDI	CATATG	1 cut(s)	479
FbaI	TGATCA	1 cut(s)	1576
FblI	GTMKAC	1 cut(s)	226
FokI	GGATG	3 cut(s)	795, 933, 1164
FriOI	GRGCYC	2 cut(s)	119, 1620
FspBI	CTAG	5 cut(s)	393, 555, 675, 696, 2402
GlaI	GCGC	1 cut(s)	2187
GsuI	CTGGAG	4 cut(s)	562, 617, 1754, 2139
HaeII	RGCGCY	1 cut(s)	2189
HaeIII	GGCC	6 cut(s)	177, 351, 753, 1151, 1907, 2081
HapII	CCGG	4 cut(s)	441, 900, 1113, 1695
HgaI	GACGC	1 cut(s)	2444
HhaI	GCGC	1 cut(s)	2188
Hin1I	GRCGYC	2 cut(s)	957, 2186
Hin6I	GCGC	1 cut(s)	2186
HinP1I	GCGC	1 cut(s)	2186
HindIII	AAGCTT	3 cut(s)	1289, 1850, 2363
HinfI	GANTC	3 cut(s)	437, 1223, 1351
HpaII	CCGG	4 cut(s)	441, 900, 1113, 1695
HphI	GGTGA	5 cut(s)	120, 1501, 1790, 2179, 2425
Hpy166II	GTNNAC	5 cut(s)	227, 264, 383, 1051, 1918
Hpy8I	GTNNAC	5 cut(s)	227, 264, 383, 1051, 1918
Hpy99I	CGWCG	2 cut(s)	959, 1414
HpyAV	CCTTC	5 cut(s)	196, 1205, 1650, 1745, 2483
HpyCH4IV	ACGT	4 cut(s)	361, 467, 957, 2031
HpyF10VI	GCNNNNNNNGC	9 cut(s)	416, 425, 566, 750, 923, 972, 1132, 1227, 2194
HpyF3I	CTNAG	7 cut(s)	35, 146, 707, 767, 783, 1614, 2058
HpySE526I	ACGT	4 cut(s)	361, 467, 957, 2031
Hsp92I	GRCGYC	2 cut(s)	957, 2186
HspAI	GCGC	1 cut(s)	2186
KasI	GGCGCC	1 cut(s)	2185
Kpn2I	TCCGGA	1 cut(s)	440
Ksp22I	TGATCA	1 cut(s)	1576
Kzo9I	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
LguI	GCTCTTC	2 cut(s)	479, 1116
LmnI	GCTCC	4 cut(s)	122, 914, 1670, 2147
LweI	GCATC	3 cut(s)	419, 569, 591
MaeI	CTAG	5 cut(s)	393, 555, 675, 696, 2402
MaeII	ACGT	4 cut(s)	361, 467, 957, 2031
MaeIII	GTNAC	6 cut(s)	677, 698, 1034, 1189, 1271, 2177
MalI	GATC	6 cut(s)	59, 802, 1578, 1893, 2043, 2445
MbiI	CCGCTC	1 cut(s)	2150
MboI	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
MfeI	CAATTG	1 cut(s)	1243
MflI	RGATCY	1 cut(s)	1891
MhlI	GDGCHC	3 cut(s)	119, 1620, 1920
MlsI	TGGCCA	1 cut(s)	2081
MluNI	TGGCCA	1 cut(s)	2081
Mly113I	GGCGCC	1 cut(s)	2186
MmeI	TCCRAC	3 cut(s)	800, 1433, 2466
Mox20I	TGGCCA	1 cut(s)	2081
Mph1103I	ATGCAT	1 cut(s)	412
MroI	TCCGGA	1 cut(s)	440
MscI	TGGCCA	1 cut(s)	2081
MseI	TTAA	7 cut(s)	104, 138, 237, 377, 1725, 1811, 1902
MslI	CAYNNNNRTG	3 cut(s)	257, 1346, 1480
Msp20I	TGGCCA	1 cut(s)	2081
MspA1I	CMGCKG	3 cut(s)	422, 582, 1016
MspCI	CTTAAG	1 cut(s)	376
MspI	CCGG	4 cut(s)	441, 900, 1113, 1695
MspR9I	CCNGG	6 cut(s)	113, 596, 1041, 1232, 1696, 2083
MunI	CAATTG	1 cut(s)	1243
Mva1269I	GAATGC	3 cut(s)	1475, 1994, 2209
MvaI	CCWGG	5 cut(s)	113, 596, 1041, 1232, 2083
MwoI	GCNNNNNNNGC	9 cut(s)	416, 425, 566, 750, 923, 972, 1132, 1227, 2194
NarI	GGCGCC	1 cut(s)	2186
NciI	CCSGG	1 cut(s)	1696
NcoI	CCATGG	1 cut(s)	540
NdeI	CATATG	1 cut(s)	479
NdeII	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
NlaIV	GGNNCC	5 cut(s)	118, 1468, 1970, 2091, 2187
NmuCI	GTSAC	1 cut(s)	1189
NsiI	ATGCAT	1 cut(s)	412
OliI	CACNNNNGTG	1 cut(s)	257
PaeR7I	CTCGAG	1 cut(s)	1501
PagI	TCATGA	1 cut(s)	2463
PceI	AGGCCT	1 cut(s)	1151
PciSI	GCTCTTC	2 cut(s)	479, 1116
PctI	GAATGC	3 cut(s)	1475, 1994, 2209
PfeI	GAWTC	3 cut(s)	437, 1223, 1351
PflFI	GACNNNGTC	1 cut(s)	708
PluTI	GGCGCC	1 cut(s)	2189
Ppu21I	YACGTR	1 cut(s)	362
PpuMI	RGGWCCY	1 cut(s)	1969
PsiI	TTATAA	1 cut(s)	1680
Psp124BI	GAGCTC	1 cut(s)	1620
Psp5II	RGGWCCY	1 cut(s)	1969
Psp6I	CCWGG	5 cut(s)	111, 594, 1039, 1230, 2081
PspGI	CCWGG	5 cut(s)	111, 594, 1039, 1230, 2081
PspN4I	GGNNCC	5 cut(s)	118, 1468, 1970, 2091, 2187
PspPI	GGNCC	2 cut(s)	805, 1969
PspPPI	RGGWCCY	1 cut(s)	1969
PsrI	GAACNNNNNNTAC	2 cut(s)	1825, 1857
PstI	CTGCAG	3 cut(s)	421, 427, 1280
PsuI	RGATCY	1 cut(s)	1891
PsyI	GACNNNGTC	1 cut(s)	708
PvuII	CAGCTG	2 cut(s)	422, 1016
RsaI	GTAC	9 cut(s)	18, 40, 772, 878, 1139, 1538, 1586, 1663, 2034
RsaNI	GTAC	9 cut(s)	17, 39, 771, 877, 1138, 1537, 1585, 1662, 2033
RseI	CAYNNNNRTG	3 cut(s)	257, 1346, 1480
SacI	GAGCTC	1 cut(s)	1620
SapI	GCTCTTC	2 cut(s)	479, 1116
SaqAI	TTAA	7 cut(s)	104, 138, 237, 377, 1725, 1811, 1902
Sau3AI	GATC	6 cut(s)	57, 800, 1576, 1891, 2041, 2443
Sau96I	GGNCC	2 cut(s)	805, 1969
ScaI	AGTACT	3 cut(s)	40, 772, 1586
ScrFI	CCNGG	6 cut(s)	113, 596, 1041, 1232, 1696, 2083
SduI	GDGCHC	3 cut(s)	119, 1620, 1920
SfaNI	GCATC	3 cut(s)	419, 569, 591
SfcI	CTRYAG	4 cut(s)	417, 423, 1276, 2233
SfoI	GGCGCC	1 cut(s)	2187
Sfr274I	CTCGAG	1 cut(s)	1501
SinI	GGWCC	2 cut(s)	805, 1969
SlaI	CTCGAG	1 cut(s)	1501
SmiMI	CAYNNNNRTG	3 cut(s)	257, 1346, 1480
SmlI	CTYRAG	2 cut(s)	376, 1501
SmoI	CTYRAG	2 cut(s)	376, 1501
SnaBI	TACGTA	1 cut(s)	362
SpeI	ACTAGT	3 cut(s)	392, 674, 695
SseBI	AGGCCT	1 cut(s)	1151
SsiI	CCGC	7 cut(s)	582, 604, 632, 812, 1135, 1889, 2150
SspDI	GGCGCC	1 cut(s)	2185
SspMI	CTAG	5 cut(s)	393, 555, 675, 696, 2402
SstI	GAGCTC	1 cut(s)	1620
StuI	AGGCCT	1 cut(s)	1151
StyD4I	CCNGG	6 cut(s)	111, 594, 1039, 1230, 1694, 2081
StyI	CCWWGG	3 cut(s)	540, 961, 1152
TaiI	ACGT	4 cut(s)	364, 470, 960, 2034
TaqI	TCGA	9 cut(s)	56, 274, 858, 1146, 1502, 1620, 1953, 2160, 2321
TatI	WGTACW	5 cut(s)	16, 38, 770, 876, 1584
TfiI	GAWTC	3 cut(s)	437, 1223, 1351
Tru1I	TTAA	7 cut(s)	104, 138, 237, 377, 1725, 1811, 1902
Tru9I	TTAA	7 cut(s)	104, 138, 237, 377, 1725, 1811, 1902
TscAI	CASTG	3 cut(s)	312, 1102, 1999
TseFI	GTSAC	1 cut(s)	1189
Tsp45I	GTSAC	1 cut(s)	1189
TspRI	CASTG	3 cut(s)	312, 1102, 1999
Tth111I	GACNNNGTC	1 cut(s)	708
Vha464I	CTTAAG	1 cut(s)	376
VneI	GTGCAC	1 cut(s)	1916
VpaK11BI	GGWCC	2 cut(s)	805, 1969
XapI	RAATTY	3 cut(s)	865, 1315, 1763
XcmI	CCANNNNNNNNNTGG	2 cut(s)	787, 968
XhoI	CTCGAG	1 cut(s)	1501
XmiI	GTMKAC	1 cut(s)	226
XspI	CTAG	5 cut(s)	393, 555, 675, 696, 2402
ZraI	GACGTC	1 cut(s)	958
ZrmI	AGTACT	3 cut(s)	40, 772, 1586
Zsp2I	ATGCAT	1 cut(s)	412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.