Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0003934

Physical Location & Seq

Genomic Coordinates Reverse (-)

59192351 .. 59194715

Gene Structure 2365 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00124760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGTTAATAACCTCAGTATTTCTTCATGGCTGTCATCTTCTGGCCGTTTCGCCTTCGGTTTCTACCCACAAAGCAACGGCTTTGCTGTTGGTGTACAGCTTCTTAATCCCACCACAAAACACAGTTGTGTGGACTGCAAATCGAGATGACCGCCCTGTTTCCCCAAACTCTACACTAAAACTAACAAGAGACGGTCTCTTGCTTCGGTCAGAGGGAGGCCAAGAAATACTTATTACTAATGGCTCAAGTTCACTAGAAACTAGTGCGATATTAAATGCATCAACTGCAGCTGCAGCTATGCTTGATTCCGGAAACTTTGTGCTGTATAATGAGAATATTTTGGTTATATGGGAGAGCTTTGATTTCCCAACTGATACAATACTGGCAGGACAAGATTTGCGGATGTCGATGGGTCTGGTATCTAGTGCATCTGTTGCAGACCACTCCAGCGGGCGATTTTCCCTGGAGATGCAGCCTGATGGAAACCTTCTAGCCTACCCGCTAAACAGTCCTTATGCTTATTGGTCTGCTATGACCTATACTAGAAGTTACCAACTTAGTCTCAACCTATCAGGCTTTCTCGTTTTGAATAGCCAATCCAGTGGTCTGAGTACTCGGGTCTTGGCAAACGGCTCGTACCATCACCCACAAGACAATCAAACCACAATCTACCGAGCAACACTTGATGTCGATGGGAATTTCAGATTGTACTTGCATAACTTTCTGATGTCAGGTAGTTCAGAGCGAGCTGTAATGCCTATTTGGTCAGCTTTGCAACATCCATGCGACGTCCATGGCATTTGTGGCTTCAACAGTTATTGTGTAGTCATGCAAAAGAAGAAAGCAGCTGAAGTTGAGTGCAAATGTTACCCTGGATTTGTTCACAACAACGCCAGTCAGAAGTCTCTGGGGTGCTATCATAATTTCACTGTAGACGGCTGCACCGGAAATGAAGAGCCACGGCAGCGGTACAAGGTAAAGGCCTTGGACAATACATCGTGGAATAATCATCCTTATTCAGTGTCACCTATTCAAAACGAGGAACATTGCAGTGACTCTTGCATGAGAGACTGCAATTGTTGGGCTGTGCTATTTACAGATGCTGATTGCAGACTATACAAGCTTCCACTTTTATATGGCAAAACAACTTCAAATATATCCTCTAAGGGTTTCTTCAAAGTGCTTCTACCGAGAAATATGGTTGGCATGCCAAACAATTTTCCCGAACCACCCAAAAGCCGGACGGTAACTGAAAGCAAGAATAATAGTCTAATTCTAATTCTTGCCATCACTCTGGTTTCACCATATACAAGCACCGAGTTCATAGGTACAAAGAAGCTCTTGAAACAGTCGAGTTTGGCATTAGCTGATCAAGAGTACTTCACTTTACAATCGTTTTCTTATGCTGAGCTCGAAAGAGCAACAGATGGCTTCAAGGAAGAGATAGGAAGGGGTACTTTCGGAGCTGTTTATAAAGGAACTCTGTCCGGGGCCAGCTCTAACAAAACTGTTGCTGTTAAAAGACTGGAGAAAGTGGTTGATGAAGGAGTAAGGGAATTTATAGCTGAAATTACCACCATTGGACGAACTCACCACAGAAACTTAATTCAGTTGCTTGGTTTCTGTATTGAGGGTTCTCGGAAGCTTCTTGTTTATGAATACATGAGCAACGGCTCACTTGCGGATCTTATCTTTAAGGCCAAAAGTGTGCACTCATCTTGGAAAGAAAGGGTAAGACTTATATTCGATGTGGCCAAAGGGGTCCTCTATTTACATGACGAATGCAGTGTCCATATCATTCATTCCAATTTGAAACCCCAAAACGTACTCTTGGATCATACTTGGACGGCTAAGATATCTGATTTCGGTTTGGCCAGGCTGGTGCCAAATGAAACAAAAATCAACTCTACTGAAGGAGAAGGAGGAGGAGCGGGAACAGTCGAACAGGTGAGAAGAGGTTACTTTGCGCCTGAATGGCAGAAGAATGCTCTCATATCTGTAAAAGCTGATATCTACAGTTTTGGTATTGTGCTTTTAGAGATTGTATGTTGTAGAAGAAACATAGAAGTAAATGTTTCAACACCAGATGAGATAATTCTTTCGAGTTGGGTCAGTAAATGCTTTGCTGCTGGAGAATTAGACAAGCTTGTGGAGGAAGATGAAAATGTAGATTTGAAGACCCTAGAAAGAATGGTGAAAGTGGGGTTGTGGTGCGTTCAAGATGATCCAGCTTTGCGTCCTTTCATGAAGAATGTAATCTTGATGTTGGAAGGGACAATGGACATACCAGTTCCACCATGTCCAGAGCTTCCCTCTCTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

774

Amino Acids

85.86

Weight (kDa)

6.23

Isoelectric Point (pI)

40.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	41 - 136	1.3e-15	D-mannose binding lectin
S_locus_glycop	PF00954	209 - 296	4.2e-06	S-locus glycoprotein domain
PK_Tyr_Ser-Thr	PF07714	478 - 752	1.6e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	479 - 751	4.4e-40	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003934)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23390
prunus_persica	Prupe.6G165100_v2.0.a1 Prupe.6G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0118681 RchiOBHm_Chr2g0118741 RchiOBHm_Chr2g0118761
rosa_laevigata	RLG00000018419
rosa_multiflora	Rmu_co8322353.1_g000001 Rmu_sc0001814.1_g000026 Rmu_sc0001814.1_g000032 Rmu_sc0002700.1_g000009 Rmu_sc0002700.1_g000033 Rmu_sc0002700.1_g000035 Rmu_sc0002831.1_g000014 Rmu_sc0002831.1_g000017 Rmu_sc0002831.1_g000031 Rmu_sc0004399.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124760 Rroxscaffold_2G00124850 Rroxscaffold_2G00124890 Rroxscaffold_2G00125410 Rroxscaffold_2G00125420
rosa_rugosa	Rorug02G0218400
rosa_samantha	Rh2AG274700 Rh2AG275000 Rh2AG275300 Rh2BG286500 Rh2BG286700 Rh2BG287100 Rh2DG301500 Rh2DG301700
rosa_wichuraiana	Rw2G021850 Rw2G021890 Rw2G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1473
AatII	GACGTC	1 cut(s)	792
AccB1I	GGYRCC	1 cut(s)	1882
AccBSI	CCGCTC	1 cut(s)	1931
AccI	GTMKAC	1 cut(s)	933
AccIII	TCCGGA	1 cut(s)	308
AciI	CCGC	7 cut(s)	151, 400, 450, 500, 967, 1682, 1931
AclWI	GGATC	3 cut(s)	1692, 1842, 2219
AcoI	YGGCCR	3 cut(s)	42, 1752, 1872
AcsI	RAATTY	2 cut(s)	697, 1556
AcuI	CTGAAG	2 cut(s)	870, 1932
AcyI	GRCGYC	1 cut(s)	789
AfaI	GTAC	9 cut(s)	95, 613, 638, 710, 971, 1330, 1379, 1456, 1827
AfiI	CCNNNNNNNGG	1 cut(s)	1239
AhlI	ACTAGT	1 cut(s)	260
AjnI	CCWGG	3 cut(s)	462, 871, 1874
AleI	CACNNNNGTG	1 cut(s)	125
Alw21I	GWGCWC	2 cut(s)	1413, 1713
Alw26I	GTCTC	5 cut(s)	183, 200, 566, 909, 1062
Alw44I	GTGCAC	1 cut(s)	1709
AlwI	GGATC	3 cut(s)	1692, 1842, 2219
AlwNI	CAGNNNCTG	1 cut(s)	1103
Ama87I	CYCGRG	1 cut(s)	615
Aor13HI	TCCGGA	1 cut(s)	308
AoxI	GGCC	7 cut(s)	42, 217, 981, 1491, 1698, 1752, 1872
ApaLI	GTGCAC	1 cut(s)	1709
ApeKI	GCWGC	8 cut(s)	287, 290, 293, 472, 845, 939, 964, 2126
ApoI	RAATTY	2 cut(s)	697, 1556
ArsI	GACNNNNNNTTYG	2 cut(s)	1773, 1805
AspLEI	GCGC	1 cut(s)	1969
AspS9I	GGNCC	2 cut(s)	1491, 1762
AsuC2I	CCSGG	1 cut(s)	1489
AsuHPI	GGTGA	6 cut(s)	635, 1017, 1293, 1583, 1960, 2206
AvaI	CYCGRG	1 cut(s)	615
AvaII	GGWCC	1 cut(s)	1762
BaeGI	GKGCMC	1 cut(s)	1713
BalI	TGGCCA	2 cut(s)	1754, 1874
BanI	GGYRCC	1 cut(s)	1882
BanII	GRGCYC	1 cut(s)	1413
BbsI	GAAGAC	1 cut(s)	2183
Bbv12I	GWGCWC	2 cut(s)	1413, 1713
BbvI	GCAGC	8 cut(s)	277, 299, 305, 484, 857, 926, 976, 2113
BccI	CCATC	6 cut(s)	403, 473, 648, 686, 1295, 1421
BceAI	ACGGC	7 cut(s)	29, 93, 646, 952, 977, 1687, 1863
BcgI	CGANNNNNNTGC	2 cut(s)	615, 649
BciT130I	CCWGG	3 cut(s)	464, 873, 1876
BclI	TGATCA	1 cut(s)	1369
BcnI	CCSGG	1 cut(s)	1489
BcoDI	GTCTC	5 cut(s)	183, 200, 566, 909, 1062
BcuI	ACTAGT	1 cut(s)	260
BfaI	CTAG	6 cut(s)	254, 261, 423, 491, 543, 2183
BfmI	CTRYAG	4 cut(s)	285, 291, 930, 2014
BglI	GCCNNNNNGGC	1 cut(s)	1975
BisI	GCNGC	8 cut(s)	288, 291, 294, 473, 846, 940, 965, 2127
BlpI	GCTNAGC	1 cut(s)	1407
BlsI	GCNGC	8 cut(s)	289, 292, 295, 474, 847, 941, 966, 2128
BmcAI	AGTACT	2 cut(s)	613, 1379
Bme1390I	CCNGG	4 cut(s)	464, 873, 1489, 1876
Bme18I	GGWCC	1 cut(s)	1762
BmeT110I	CYCGRG	1 cut(s)	615
BmgT120I	GGNCC	2 cut(s)	1491, 1762
BmiI	GGNNCC	3 cut(s)	1492, 1763, 1884
BmrFI	CCNGG	4 cut(s)	464, 873, 1489, 1876
BmsI	GCATC	4 cut(s)	287, 437, 459, 1090
BpiI	GAAGAC	1 cut(s)	2183
BplI	GAGNNNNNCTC	3 cut(s)	180, 212, 2297
BpmI	CTGGAG	4 cut(s)	430, 485, 1547, 2151
Bpu1102I	GCTNAGC	1 cut(s)	1407
BpuEI	CTTGAG	1 cut(s)	229
BpuMI	CCSGG	1 cut(s)	1489
BsaHI	GRCGYC	1 cut(s)	789
BsaI	GGTCTC	1 cut(s)	200
BsaJI	CCNNGG	6 cut(s)	462, 793, 871, 959, 984, 1488
BsaWI	WCCGGW	2 cut(s)	308, 944
Bsc4I	CCNNNNNNNGG	1 cut(s)	1239
Bse1I	ACTGG	5 cut(s)	387, 600, 894, 1530, 2288
Bse3DI	GCAATG	1 cut(s)	1045
BseAI	TCCGGA	1 cut(s)	308
BseBI	CCWGG	3 cut(s)	464, 873, 1876
BseDI	CCNNGG	6 cut(s)	462, 793, 871, 959, 984, 1488
BseGI	GGATG	3 cut(s)	408, 778, 1009
BseLI	CCNNNNNNNGG	1 cut(s)	1239
BseMI	GCAATG	1 cut(s)	1045
BseMII	CTCAG	3 cut(s)	27, 599, 1398
BseNI	ACTGG	5 cut(s)	387, 600, 894, 1530, 2288
BseRI	GAGGAG	2 cut(s)	1938, 1941
BseSI	GKGCMC	1 cut(s)	1713
BseXI	GCAGC	8 cut(s)	277, 299, 305, 484, 857, 926, 976, 2113
BsgI	GTGCAG	1 cut(s)	925
BshFI	GGCC	7 cut(s)	44, 219, 983, 1493, 1700, 1754, 1874
BshNI	GGYRCC	1 cut(s)	1882
BsiHKAI	GWGCWC	2 cut(s)	1413, 1713
BsiHKCI	CYCGRG	1 cut(s)	615
BsiSI	CCGG	4 cut(s)	309, 945, 1240, 1488
BslFI	GGGAC	1 cut(s)	2287
BslI	CCNNNNNNNGG	1 cut(s)	1239
BsmAI	GTCTC	5 cut(s)	183, 200, 566, 909, 1062
BsmBI	CGTCTC	1 cut(s)	183
BsmFI	GGGAC	1 cut(s)	2287
BsmI	GAATGC	2 cut(s)	1787, 1990
BsnI	GGCC	7 cut(s)	44, 219, 983, 1493, 1700, 1754, 1874
Bso31I	GGTCTC	1 cut(s)	200
BsoBI	CYCGRG	1 cut(s)	615
Bsp1286I	GDGCHC	2 cut(s)	1413, 1713
Bsp13I	TCCGGA	1 cut(s)	308
Bsp1407I	TGTACA	1 cut(s)	93
Bsp143I	GATC	4 cut(s)	1369, 1684, 1834, 2224
Bsp1720I	GCTNAGC	1 cut(s)	1407
Bsp19I	CCATGG	1 cut(s)	793
BspACI	CCGC	7 cut(s)	151, 400, 450, 500, 967, 1682, 1931
BspANI	GGCC	7 cut(s)	44, 219, 983, 1493, 1700, 1754, 1874
BspCNI	CTCAG	3 cut(s)	26, 600, 1399
BspEI	TCCGGA	1 cut(s)	308
BspHI	TCATGA	1 cut(s)	2244
BspLI	GGNNCC	3 cut(s)	1492, 1763, 1884
BspMAI	CTGCAG	2 cut(s)	289, 295
BspPI	GGATC	3 cut(s)	1692, 1842, 2219
BspQI	GCTCTTC	1 cut(s)	948
BspT107I	GGYRCC	1 cut(s)	1882
BspTNI	GGTCTC	1 cut(s)	200
BsrBI	CCGCTC	1 cut(s)	1931
BsrDI	GCAATG	1 cut(s)	1045
BsrGI	TGTACA	1 cut(s)	93
BsrI	ACTGG	5 cut(s)	387, 600, 894, 1530, 2288
BssECI	CCNNGG	6 cut(s)	462, 793, 871, 959, 984, 1488
BssMI	GATC	4 cut(s)	1369, 1684, 1834, 2224
BssNI	GRCGYC	1 cut(s)	789
BssT1I	CCWWGG	2 cut(s)	793, 984
Bst2UI	CCWGG	3 cut(s)	464, 873, 1876
Bst6I	CTCTTC	3 cut(s)	948, 1434, 1948
BstACI	GRCGYC	1 cut(s)	789
BstAPI	GCANNNNNTGC	2 cut(s)	284, 434
BstAUI	TGTACA	1 cut(s)	93
BstC8I	GCNNGC	4 cut(s)	452, 747, 1208, 1495
BstDEI	CTNAG	6 cut(s)	13, 557, 608, 1164, 1407, 1851
BstDSI	CCRYGG	2 cut(s)	793, 959
BstF5I	GGATG	3 cut(s)	408, 778, 1009
BstHHI	GCGC	1 cut(s)	1969
BstKTI	GATC	4 cut(s)	1372, 1687, 1837, 2227
BstMAI	GTCTC	5 cut(s)	183, 200, 566, 909, 1062
BstMBI	GATC	4 cut(s)	1369, 1684, 1834, 2224
BstMWI	GCNNNNNNNGC	6 cut(s)	284, 293, 434, 804, 964, 1975
BstNI	CCWGG	3 cut(s)	464, 873, 1876
BstNSI	RCATGY	1 cut(s)	1210
BstSCI	CCNGG	4 cut(s)	462, 871, 1487, 1874
BstSFI	CTRYAG	4 cut(s)	285, 291, 930, 2014
BstSLI	GKGCMC	1 cut(s)	1713
BstV1I	GCAGC	8 cut(s)	277, 299, 305, 484, 857, 926, 976, 2113
BstV2I	GAAGAC	1 cut(s)	2183
BstX2I	RGATCY	1 cut(s)	1684
BstXI	CCANNNNNNTGG	2 cut(s)	602, 1294
BstYI	RGATCY	1 cut(s)	1684
BsuRI	GGCC	7 cut(s)	44, 219, 983, 1493, 1700, 1754, 1874
BtgI	CCRYGG	2 cut(s)	793, 959
BtsCI	GGATG	3 cut(s)	408, 778, 1009
BtsI	GCAGTG	2 cut(s)	1057, 1792
BtsIMutI	CAGTG	5 cut(s)	607, 927, 1026, 1057, 1792
Cac8I	GCNNGC	4 cut(s)	452, 747, 1208, 1495
CaiI	CAGNNNCTG	1 cut(s)	1103
CciI	TCATGA	1 cut(s)	2244
CfoI	GCGC	1 cut(s)	1969
Cfr13I	GGNCC	2 cut(s)	1491, 1762
CseI	GACGC	1 cut(s)	2225
Csp6I	GTAC	9 cut(s)	94, 612, 637, 709, 970, 1329, 1378, 1455, 1826
CviQI	GTAC	9 cut(s)	94, 612, 637, 709, 970, 1329, 1378, 1455, 1826
DdeI	CTNAG	6 cut(s)	13, 557, 608, 1164, 1407, 1851
DpnI	GATC	4 cut(s)	1371, 1686, 1836, 2226
DpnII	GATC	4 cut(s)	1369, 1684, 1834, 2224
EaeI	YGGCCR	3 cut(s)	42, 1752, 1872
Eam1104I	CTCTTC	3 cut(s)	948, 1434, 1948
EarI	CTCTTC	3 cut(s)	948, 1434, 1948
Ecl136II	GAGCTC	1 cut(s)	1411
Eco130I	CCWWGG	2 cut(s)	793, 984
Eco147I	AGGCCT	1 cut(s)	983
Eco24I	GRGCYC	1 cut(s)	1413
Eco31I	GGTCTC	1 cut(s)	200
Eco32I	GATATC	2 cut(s)	1857, 2011
Eco47I	GGWCC	1 cut(s)	1762
Eco53kI	GAGCTC	1 cut(s)	1411
Eco57I	CTGAAG	2 cut(s)	870, 1932
Eco88I	CYCGRG	1 cut(s)	615
EcoICRI	GAGCTC	1 cut(s)	1411
EcoO109I	RGGNCCY	1 cut(s)	1762
EcoRII	CCWGG	3 cut(s)	462, 871, 1874
EcoRV	GATATC	2 cut(s)	1857, 2011
EcoT14I	CCWWGG	2 cut(s)	793, 984
EcoT22I	ATGCAT	1 cut(s)	280
EcoT38I	GRGCYC	1 cut(s)	1413
ErhI	CCWWGG	2 cut(s)	793, 984
Esp3I	CGTCTC	1 cut(s)	183
FalI	AAGNNNNNCTT	8 cut(s)	213, 245, 1157, 1189, 1325, 1357, 1441, 1473
FaqI	GGGAC	1 cut(s)	2287
FauI	CCCGC	3 cut(s)	443, 507, 1924
FbaI	TGATCA	1 cut(s)	1369
FblI	GTMKAC	1 cut(s)	933
Fnu4HI	GCNGC	8 cut(s)	288, 291, 294, 473, 846, 940, 965, 2127
FokI	GGATG	3 cut(s)	415, 765, 996
FriOI	GRGCYC	1 cut(s)	1413
Fsp4HI	GCNGC	8 cut(s)	288, 291, 294, 473, 846, 940, 965, 2127
FspBI	CTAG	6 cut(s)	254, 261, 423, 491, 543, 2183
GlaI	GCGC	1 cut(s)	1968
GluI	GCNGC	8 cut(s)	288, 291, 294, 473, 846, 940, 965, 2127
GsuI	CTGGAG	4 cut(s)	430, 485, 1547, 2151
HaeIII	GGCC	7 cut(s)	44, 219, 983, 1493, 1700, 1754, 1874
HapII	CCGG	4 cut(s)	309, 945, 1240, 1488
HgaI	GACGC	1 cut(s)	2225
HhaI	GCGC	1 cut(s)	1969
Hin1I	GRCGYC	1 cut(s)	789
Hin6I	GCGC	1 cut(s)	1967
HinP1I	GCGC	1 cut(s)	1967
HindIII	AAGCTT	3 cut(s)	1121, 1643, 2144
HinfI	GANTC	2 cut(s)	305, 1055
HpaII	CCGG	4 cut(s)	309, 945, 1240, 1488
HphI	GGTGA	6 cut(s)	635, 1017, 1293, 1583, 1960, 2206
Hpy166II	GTNNAC	6 cut(s)	94, 132, 251, 883, 934, 1711
Hpy188I	TCNGA	9 cut(s)	211, 609, 704, 726, 742, 900, 1463, 1641, 1861
Hpy188III	TCNNGA	9 cut(s)	143, 309, 1223, 1342, 1373, 2219, 2245, 2260, 2303
Hpy8I	GTNNAC	6 cut(s)	94, 132, 251, 883, 934, 1711
Hpy99I	CGWCG	1 cut(s)	791
HpyAV	CCTTC	7 cut(s)	63, 497, 1443, 1538, 1907, 1913, 2264
HpyCH4IV	ACGT	2 cut(s)	789, 1824
HpyF10VI	GCNNNNNNNGC	6 cut(s)	284, 293, 434, 804, 964, 1975
HpyF3I	CTNAG	6 cut(s)	13, 557, 608, 1164, 1407, 1851
HpySE526I	ACGT	2 cut(s)	789, 1824
Hsp92I	GRCGYC	1 cut(s)	789
HspAI	GCGC	1 cut(s)	1967
Kpn2I	TCCGGA	1 cut(s)	308
Ksp22I	TGATCA	1 cut(s)	1369
Kzo9I	GATC	4 cut(s)	1369, 1684, 1834, 2224
LguI	GCTCTTC	1 cut(s)	948
LmnI	GCTCC	2 cut(s)	1463, 1928
Lsp1109I	GCAGC	8 cut(s)	277, 299, 305, 484, 857, 926, 976, 2113
LweI	GCATC	4 cut(s)	287, 437, 459, 1090
MaeI	CTAG	6 cut(s)	254, 261, 423, 491, 543, 2183
MaeII	ACGT	2 cut(s)	789, 1824
MaeIII	GTNAC	6 cut(s)	548, 866, 1023, 1052, 1246, 1958
MalI	GATC	4 cut(s)	1371, 1686, 1836, 2226
MbiI	CCGCTC	1 cut(s)	1931
MboI	GATC	4 cut(s)	1369, 1684, 1834, 2224
MfeI	CAATTG	1 cut(s)	1075
MflI	RGATCY	1 cut(s)	1684
MhlI	GDGCHC	2 cut(s)	1413, 1713
MlsI	TGGCCA	2 cut(s)	1754, 1874
MluNI	TGGCCA	2 cut(s)	1754, 1874
MlyI	GAGTC	1 cut(s)	1049
MmeI	TCCRAC	1 cut(s)	2247
Mox20I	TGGCCA	2 cut(s)	1754, 1874
Mph1103I	ATGCAT	1 cut(s)	280
MroI	TCCGGA	1 cut(s)	308
MscI	TGGCCA	2 cut(s)	1754, 1874
MseI	TTAA	6 cut(s)	5, 104, 272, 1518, 1604, 1695
MslI	CAYNNNNRTG	2 cut(s)	125, 1050
Msp20I	TGGCCA	2 cut(s)	1754, 1874
MspA1I	CMGCKG	4 cut(s)	290, 450, 848, 967
MspI	CCGG	4 cut(s)	309, 945, 1240, 1488
MspR9I	CCNGG	4 cut(s)	464, 873, 1489, 1876
MunI	CAATTG	1 cut(s)	1075
Mva1269I	GAATGC	2 cut(s)	1787, 1990
MvaI	CCWGG	3 cut(s)	464, 873, 1876
MwoI	GCNNNNNNNGC	6 cut(s)	284, 293, 434, 804, 964, 1975
NciI	CCSGG	1 cut(s)	1489
NcoI	CCATGG	1 cut(s)	793
NdeII	GATC	4 cut(s)	1369, 1684, 1834, 2224
NlaIV	GGNNCC	3 cut(s)	1492, 1763, 1884
NmuCI	GTSAC	2 cut(s)	1023, 1052
NsiI	ATGCAT	1 cut(s)	280
NspI	RCATGY	1 cut(s)	1210
OliI	CACNNNNGTG	1 cut(s)	125
PaeI	GCATGC	1 cut(s)	1210
PagI	TCATGA	1 cut(s)	2244
PceI	AGGCCT	1 cut(s)	983
PciSI	GCTCTTC	1 cut(s)	948
PctI	GAATGC	2 cut(s)	1787, 1990
PfeI	GAWTC	1 cut(s)	305
PkrI	GCNGC	8 cut(s)	289, 292, 295, 474, 847, 941, 966, 2128
PleI	GAGTC	1 cut(s)	1049
PpsI	GAGTC	1 cut(s)	1049
PpuMI	RGGWCCY	1 cut(s)	1762
PsiI	TTATAA	1 cut(s)	1473
Psp124BI	GAGCTC	1 cut(s)	1413
Psp5II	RGGWCCY	1 cut(s)	1762
Psp6I	CCWGG	3 cut(s)	462, 871, 1874
PspGI	CCWGG	3 cut(s)	462, 871, 1874
PspN4I	GGNNCC	3 cut(s)	1492, 1763, 1884
PspPI	GGNCC	2 cut(s)	1491, 1762
PspPPI	RGGWCCY	1 cut(s)	1762
PsrI	GAACNNNNNNTAC	2 cut(s)	1618, 1650
PstI	CTGCAG	2 cut(s)	289, 295
PstNI	CAGNNNCTG	1 cut(s)	1103
PsuI	RGATCY	1 cut(s)	1684
PvuII	CAGCTG	2 cut(s)	290, 848
RsaI	GTAC	9 cut(s)	95, 613, 638, 710, 971, 1330, 1379, 1456, 1827
RsaNI	GTAC	9 cut(s)	94, 612, 637, 709, 970, 1329, 1378, 1455, 1826
RseI	CAYNNNNRTG	2 cut(s)	125, 1050
SacI	GAGCTC	1 cut(s)	1413
SapI	GCTCTTC	1 cut(s)	948
SaqAI	TTAA	6 cut(s)	5, 104, 272, 1518, 1604, 1695
SatI	GCNGC	8 cut(s)	288, 291, 294, 473, 846, 940, 965, 2127
Sau3AI	GATC	4 cut(s)	1369, 1684, 1834, 2224
Sau96I	GGNCC	2 cut(s)	1491, 1762
ScaI	AGTACT	2 cut(s)	613, 1379
SchI	GAGTC	1 cut(s)	1049
ScrFI	CCNGG	4 cut(s)	464, 873, 1489, 1876
SduI	GDGCHC	2 cut(s)	1413, 1713
SfaNI	GCATC	4 cut(s)	287, 437, 459, 1090
SfcI	CTRYAG	4 cut(s)	285, 291, 930, 2014
SinI	GGWCC	1 cut(s)	1762
SmiMI	CAYNNNNRTG	2 cut(s)	125, 1050
SmlI	CTYRAG	1 cut(s)	244
SmoI	CTYRAG	1 cut(s)	244
SpeI	ACTAGT	1 cut(s)	260
SphI	GCATGC	1 cut(s)	1210
SseBI	AGGCCT	1 cut(s)	983
SsiI	CCGC	7 cut(s)	151, 400, 450, 500, 967, 1682, 1931
SspI	AATATT	1 cut(s)	337
SspMI	CTAG	6 cut(s)	254, 261, 423, 491, 543, 2183
SstI	GAGCTC	1 cut(s)	1413
StuI	AGGCCT	1 cut(s)	983
StyD4I	CCNGG	4 cut(s)	462, 871, 1487, 1874
StyI	CCWWGG	2 cut(s)	793, 984
TaiI	ACGT	2 cut(s)	792, 1827
TaqI	TCGA	8 cut(s)	142, 407, 690, 1352, 1413, 1746, 1941, 2102
TaqII	GACCGA	1 cut(s)	195
TatI	WGTACW	4 cut(s)	93, 611, 708, 1377
TfiI	GAWTC	1 cut(s)	305
Tru1I	TTAA	6 cut(s)	5, 104, 272, 1518, 1604, 1695
Tru9I	TTAA	6 cut(s)	5, 104, 272, 1518, 1604, 1695
TscAI	CASTG	5 cut(s)	607, 934, 1026, 1057, 1792
TseFI	GTSAC	2 cut(s)	1023, 1052
TseI	GCWGC	8 cut(s)	287, 290, 293, 472, 845, 939, 964, 2126
Tsp45I	GTSAC	2 cut(s)	1023, 1052
TspRI	CASTG	5 cut(s)	607, 934, 1026, 1057, 1792
VneI	GTGCAC	1 cut(s)	1709
VpaK11BI	GGWCC	1 cut(s)	1762
XapI	RAATTY	2 cut(s)	697, 1556
XceI	RCATGY	1 cut(s)	1210
XcmI	CCANNNNNNNNNTGG	1 cut(s)	800
XmiI	GTMKAC	1 cut(s)	933
XspI	CTAG	6 cut(s)	254, 261, 423, 491, 543, 2183
ZraI	GACGTC	1 cut(s)	790
ZrmI	AGTACT	2 cut(s)	613, 1379
Zsp2I	ATGCAT	1 cut(s)	280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.