RLG00000021400

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004529
Physical Location & Seq
Forward (+)
74865522 .. 74867762
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021400

Sequence Viewer

Length: 1386 bp
ATGACTCAGATACGTCCATCCTCCATACCGAATAACATGCACCTCCAGGATCTGCTAGTGGATATCCTTGTAACTTGGGATCTGGAGACCGCCACCGCCGGAAGCGTTCAACGCCGTTTCATCCAAGAATATATCGATCTCTTGTTTGATGATGGGAAAGAGGATGTGAATGAAAGCAAGGGTACAAAAAGAAATAGAAAGAGAAAGGCGCCGGAGATATGTGGTGTCTCCCCGCAGCTTCAGGAGCTTGTAGGAGGAGGATCCGAAATGATGCGAGCGGAGGCTGTGAAGAAAATTGTGGCTTATAGCCGGGAAAAGGGTTTGCTAACTGCAAAGAGGATCATAGTAGCAGATGATAAACTAGATGCCCTTCTTCCCAAATATAAATTTATTTTTATATTACAAATAAGTGACCTCTTGTCCCGGCATGGGCATATGCAGCCGCTTAAAGTTGGCAAAGATGATGGCGGTGTGAGGAAGAGGAAGAGGAACAACGAAGCAAATGGTAAAAGATCTGAGCATTGTGAAGGTGATCAAGTTAGGGTGAAAAGGAAGAAGAACTATATTAAACGAAACATTAACCTCGCACTAGCAGAGGAAGATGTGCGACCGTGGGATAAAATGCCAGAAGATATTATGGTAAAAATCTTTAAGCTATTACCATTTCCATATTGGTCATTCTATCCCTGTTATGTCTGCCGTTCATGGCAATTGGCAGTGGCCGATGTAATGTTTCCACCTAATAACAATGAGCTTGATCTGCGGCTCTTTGATTCATATCCCTGGAAATCAAGGCAGAGGGTTCTACTCCTATACCTCAGGATTGTCTTAAATTCCCGACCCACCATCCCTTGGGCAACACTTTACTTGCCCGAGAAGACTGCATTCATCACCAATGAAGTCCTCACTTGGATCGCTCAAAAGACACCGGAACTACTTAATCTTTCTGTACCTAAAAAGTTGAGGGGAGATGTTTTTCTTGAGTCGGTTACACCTAAGTGGAAGAATTTGAGAATGGTGCACATCGATATTTCTGCAAATAATTTTTCTCATCTCCAAGCTAATTGCACATTTATAAGTGAGCTTAGTTTATATGGATGGATGGACGATGATTCAGCATCCATGCTAGCTAAATCTTTTCCAAATCTCAAGTCTCTAGAGATTTATGGGTGTACAATGTCGAACCATACCCTAGGAATCATAATGGATGGTCACAAGAATCTCAATCTAGTTGCTACACGAAAGAAGAACTTCTACCACCATGTTGACCTACTCGGTCACAAGTTGGTTGAGGCACTTACACGAGGAAACTACTACCGTTTTGAGACTTGTGTGGAGCTGGAGTTGGAAAGAACATTTGCTGGGTTTAACCCTATATATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

462

Amino Acids

53.33

Weight (kDa)

9.22

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 74 - 143 2.4e-11 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1076
AccB1I GGYRCC 1 cut(s) 208
AccB7I CCANNNNNTGG 1 cut(s) 852
AccBSI CCGCTC 1 cut(s) 278
AciI CCGC 7 cut(s) 90, 96, 233, 278, 443, 468, 763
AclWI GGATC 6 cut(s) 57, 87, 255, 268, 347, 920
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 3 cut(s) 386, 832, 1006
AcuI CTGAAG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 209
AfaI GTAC 3 cut(s) 184, 951, 1174
AfiI CCNNNNNNNGG 3 cut(s) 316, 429, 852
AgsI TTSAA 1 cut(s) 110
AhdI GACNNNNNGTC 1 cut(s) 418
AjnI CCWGG 2 cut(s) 45, 782
AleI CACNNNNGTG 1 cut(s) 997
AluBI AGCT 8 cut(s) 238, 247, 655, 754, 1061, 1084, 1130, 1339
AluI AGCT 8 cut(s) 238, 247, 655, 754, 1061, 1084, 1130, 1339
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 4 cut(s) 80, 232, 1158, 1319
Alw44I GTGCAC 1 cut(s) 1019
AlwI GGATC 6 cut(s) 57, 87, 255, 268, 347, 920
AlwNI CAGNNNCTG 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 872
AoxI GGCC 1 cut(s) 720
ApaLI GTGCAC 1 cut(s) 1019
ApeKI GCWGC 2 cut(s) 235, 439
ApoI RAATTY 3 cut(s) 386, 832, 1006
ArsI GACNNNNNNTTYG 2 cut(s) 1136, 1168
Asp700I GAANNNNTTC 1 cut(s) 1250
AspA2I CCTAGG 1 cut(s) 1192
AspLEI GCGC 1 cut(s) 211
AsuC2I CCSGG 2 cut(s) 311, 424
AsuHPI GGTGA 3 cut(s) 542, 556, 883
AsuNHI GCTAGC 1 cut(s) 1126
AvaI CYCGRG 1 cut(s) 872
AvrII CCTAGG 1 cut(s) 1192
AxyI CCTNAGG 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 1023
BamHI GGATCC 1 cut(s) 260
BanI GGYRCC 1 cut(s) 208
BarI GAAGNNNNNNTAC 2 cut(s) 1238, 1270
BauI CACGAG 1 cut(s) 1302
BbsI GAAGAC 1 cut(s) 884
Bbv12I GWGCWC 1 cut(s) 1023
BbvI GCAGC 2 cut(s) 247, 451
BccI CCATC 7 cut(s) 25, 146, 458, 854, 1092, 1096, 1202
BceAI ACGGC 2 cut(s) 99, 684
BcgI CGANNNNNNTGC 6 cut(s) 19, 53, 863, 897, 1016, 1050
BciT130I CCWGG 2 cut(s) 47, 784
BclI TGATCA 1 cut(s) 532
BcnI CCSGG 2 cut(s) 311, 424
BcoDI GTCTC 4 cut(s) 80, 232, 1158, 1319
BfaI CTAG 7 cut(s) 56, 362, 590, 1127, 1157, 1193, 1229
BfoI RGCGCY 1 cut(s) 212
BglII AGATCT 1 cut(s) 512
BisI GCNGC 4 cut(s) 236, 440, 443, 764
BlnI CCTAGG 1 cut(s) 1192
BlsI GCNGC 4 cut(s) 237, 441, 444, 765
Bme1390I CCNGG 4 cut(s) 47, 311, 424, 784
BmeRI GACNNNNNGTC 1 cut(s) 418
BmeT110I CYCGRG 1 cut(s) 872
BmiI GGNNCC 2 cut(s) 210, 262
BmrFI CCNGG 4 cut(s) 47, 311, 424, 784
BmsI GCATC 3 cut(s) 261, 355, 1127
BmtI GCTAGC 1 cut(s) 1130
BpiI GAAGAC 1 cut(s) 884
BpmI CTGGAG 3 cut(s) 29, 104, 1361
BpuEI CTTGAG 2 cut(s) 1001, 1133
BpuMI CCSGG 2 cut(s) 311, 424
Bsa29I ATCGAT 2 cut(s) 135, 1026
BsaBI GATNNNNATC 1 cut(s) 777
BsaHI GRCGYC 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 4 cut(s) 611, 782, 851, 1192
BsaWI WCCGGW 1 cut(s) 928
BsaXI ACNNNNNCTCC 2 cut(s) 1328, 1358
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 429, 852
Bse21I CCTNAGG 1 cut(s) 818
Bse8I GATNNNNATC 1 cut(s) 777
BseBI CCWGG 2 cut(s) 47, 784
BseCI ATCGAT 2 cut(s) 135, 1026
BseDI CCNNGG 4 cut(s) 611, 782, 851, 1192
BseGI GGATG 8 cut(s) 17, 120, 169, 846, 1103, 1107, 1118, 1213
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 3 cut(s) 316, 429, 852
BseMII CTCAG 3 cut(s) 20, 507, 832
BseRI GAGGAG 1 cut(s) 270
BseSI GKGCMC 1 cut(s) 1023
BseXI GCAGC 2 cut(s) 247, 451
BseYI CCCAGC 1 cut(s) 1361
Bsh1285I CGRYCG 1 cut(s) 611
BshFI GGCC 1 cut(s) 722
BshNI GGYRCC 1 cut(s) 208
BshVI ATCGAT 2 cut(s) 135, 1026
BsiEI CGRYCG 1 cut(s) 611
BsiHKAI GWGCWC 1 cut(s) 1023
BsiHKCI CYCGRG 1 cut(s) 872
BsiSI CCGG 5 cut(s) 99, 212, 310, 424, 929
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 3 cut(s) 316, 429, 852
BsmAI GTCTC 4 cut(s) 80, 232, 1158, 1319
BsmFI GGGAC 1 cut(s) 406
BsmI GAATGC 1 cut(s) 884
BsnI GGCC 1 cut(s) 722
Bso31I GGTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 872
Bsp1286I GDGCHC 1 cut(s) 1023
Bsp1407I TGTACA 1 cut(s) 1172
Bsp143I GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
BspACI CCGC 7 cut(s) 90, 96, 233, 278, 443, 468, 763
BspANI GGCC 1 cut(s) 722
BspCNI CTCAG 3 cut(s) 19, 508, 831
BspDI ATCGAT 2 cut(s) 135, 1026
BspLI GGNNCC 2 cut(s) 210, 262
BspOI GCTAGC 1 cut(s) 1130
BspPI GGATC 6 cut(s) 57, 87, 255, 268, 347, 920
BspT107I GGYRCC 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 278
BsrGI TGTACA 1 cut(s) 1172
BssECI CCNNGG 4 cut(s) 611, 782, 851, 1192
BssMI GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
BssNI GRCGYC 1 cut(s) 209
BssSI CACGAG 1 cut(s) 1302
BssT1I CCWWGG 2 cut(s) 851, 1192
Bst2BI CACGAG 1 cut(s) 1302
Bst2UI CCWGG 2 cut(s) 47, 784
Bst4CI ACNGT 2 cut(s) 612, 1319
Bst6I CTCTTC 2 cut(s) 473, 479
BstACI GRCGYC 1 cut(s) 209
BstAUI TGTACA 1 cut(s) 1172
BstC8I GCNNGC 2 cut(s) 276, 1128
BstDEI CTNAG 5 cut(s) 6, 516, 818, 996, 1085
BstDSI CCRYGG 1 cut(s) 611
BstF5I GGATG 8 cut(s) 17, 120, 169, 846, 1103, 1107, 1118, 1213
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 9 cut(s) 52, 82, 139, 263, 342, 515, 535, 760, 915
BstMAI GTCTC 4 cut(s) 80, 232, 1158, 1319
BstMBI GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
BstMCI CGRYCG 1 cut(s) 611
BstMWI GCNNNNNNNGC 4 cut(s) 111, 244, 439, 760
BstNI CCWGG 2 cut(s) 47, 784
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 4 cut(s) 45, 309, 422, 782
BstSLI GKGCMC 1 cut(s) 1023
BstV1I GCAGC 2 cut(s) 247, 451
BstV2I GAAGAC 1 cut(s) 884
BstX2I RGATCY 4 cut(s) 49, 79, 260, 512
BstYI RGATCY 4 cut(s) 49, 79, 260, 512
Bsu15I ATCGAT 2 cut(s) 135, 1026
Bsu36I CCTNAGG 1 cut(s) 818
BsuRI GGCC 1 cut(s) 722
BsuTUI ATCGAT 2 cut(s) 135, 1026
BtgI CCRYGG 1 cut(s) 611
BtsCI GGATG 8 cut(s) 17, 120, 169, 846, 1103, 1107, 1118, 1213
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 2 cut(s) 723, 1381
Cac8I GCNNGC 2 cut(s) 276, 1128
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 211
ClaI ATCGAT 2 cut(s) 135, 1026
Csp6I GTAC 3 cut(s) 183, 950, 1173
CviAII CATG 5 cut(s) 37, 428, 705, 1123, 1262
CviQI GTAC 3 cut(s) 183, 950, 1173
DdeI CTNAG 5 cut(s) 6, 516, 818, 996, 1085
DinI GGCGCC 1 cut(s) 210
DpnI GATC 9 cut(s) 51, 81, 138, 262, 341, 514, 534, 759, 914
DpnII GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
DriI GACNNNNNGTC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 2 cut(s) 473, 479
Eam1105I GACNNNNNGTC 1 cut(s) 418
EarI CTCTTC 2 cut(s) 473, 479
Eco130I CCWWGG 2 cut(s) 851, 1192
Eco31I GGTCTC 1 cut(s) 80
Eco32I GATATC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 224
Eco81I CCTNAGG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 872
EcoRII CCWGG 2 cut(s) 45, 782
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 2 cut(s) 851, 1192
EgeI GGCGCC 1 cut(s) 210
EheI GGCGCC 1 cut(s) 210
ErhI CCWWGG 2 cut(s) 851, 1192
FaeI CATG 5 cut(s) 40, 431, 708, 1126, 1265
FalI AAGNNNNNCTT 2 cut(s) 1235, 1267
FaqI GGGAC 1 cut(s) 406
FatI CATG 5 cut(s) 36, 427, 704, 1122, 1261
FauI CCCGC 1 cut(s) 240
FauNDI CATATG 1 cut(s) 435
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 4 cut(s) 236, 440, 443, 764
FokI GGATG 8 cut(s) 4, 107, 176, 833, 1105, 1110, 1114, 1220
Fsp4HI GCNGC 4 cut(s) 236, 440, 443, 764
FspBI CTAG 7 cut(s) 56, 362, 590, 1127, 1157, 1193, 1229
GlaI GCGC 1 cut(s) 210
GluI GCNGC 4 cut(s) 236, 440, 443, 764
GsaI CCCAGC 1 cut(s) 1365
GsuI CTGGAG 3 cut(s) 29, 104, 1361
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 1 cut(s) 722
HapII CCGG 5 cut(s) 99, 212, 310, 424, 929
HhaI GCGC 1 cut(s) 211
Hin1I GRCGYC 1 cut(s) 209
Hin1II CATG 5 cut(s) 40, 431, 708, 1126, 1265
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HincII GTYRAC 1 cut(s) 1267
HindII GTYRAC 1 cut(s) 1267
HinfI GANTC 6 cut(s) 4, 773, 983, 1112, 1197, 1219
HpaII CCGG 5 cut(s) 99, 212, 310, 424, 929
HphI GGTGA 3 cut(s) 542, 556, 883
Hpy166II GTNNAC 3 cut(s) 1021, 1173, 1267
Hpy188I TCNGA 3 cut(s) 9, 265, 517
Hpy188III TCNNGA 6 cut(s) 83, 242, 820, 837, 980, 1157
Hpy8I GTNNAC 3 cut(s) 1021, 1173, 1267
HpyAV CCTTC 2 cut(s) 380, 521
HpyCH4III ACNGT 2 cut(s) 612, 1319
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 7 cut(s) 40, 332, 439, 884, 1021, 1037, 1068
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 244, 439, 760
HpyF3I CTNAG 5 cut(s) 6, 516, 818, 996, 1085
HpySE526I ACGT 1 cut(s) 13
Hsp92I GRCGYC 1 cut(s) 209
Hsp92II CATG 5 cut(s) 40, 431, 708, 1126, 1265
HspAI GCGC 1 cut(s) 209
KasI GGCGCC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
LmnI GCTCC 2 cut(s) 244, 1336
Lsp1109I GCAGC 2 cut(s) 247, 451
LweI GCATC 3 cut(s) 261, 355, 1127
MaeI CTAG 7 cut(s) 56, 362, 590, 1127, 1157, 1193, 1229
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 5 cut(s) 70, 410, 988, 1211, 1277
MalI GATC 9 cut(s) 51, 81, 138, 262, 341, 514, 534, 759, 914
MbiI CCGCTC 1 cut(s) 278
MboI GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
MfeI CAATTG 1 cut(s) 710
MflI RGATCY 4 cut(s) 49, 79, 260, 512
MhlI GDGCHC 1 cut(s) 1023
MluCI AATT 7 cut(s) 294, 386, 710, 832, 1006, 1042, 1063
Mly113I GGCGCC 1 cut(s) 209
MlyI GAGTC 1 cut(s) 992
MmeI TCCRAC 1 cut(s) 1326
MroXI GAANNNNTTC 1 cut(s) 1250
MseI TTAA 7 cut(s) 447, 567, 579, 651, 830, 939, 1368
MslI CAYNNNNRTG 1 cut(s) 997
MspI CCGG 5 cut(s) 99, 212, 310, 424, 929
MspR9I CCNGG 4 cut(s) 47, 311, 424, 784
MunI CAATTG 1 cut(s) 710
Mva1269I GAATGC 1 cut(s) 884
MvaI CCWGG 2 cut(s) 47, 784
MwoI GCNNNNNNNGC 4 cut(s) 111, 244, 439, 760
NarI GGCGCC 1 cut(s) 209
NciI CCSGG 2 cut(s) 311, 424
NdeI CATATG 1 cut(s) 435
NdeII GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
NheI GCTAGC 1 cut(s) 1126
NlaIII CATG 5 cut(s) 40, 431, 708, 1126, 1265
NlaIV GGNNCC 2 cut(s) 210, 262
NmuCI GTSAC 3 cut(s) 410, 1211, 1277
NspI RCATGY 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 997
PctI GAATGC 1 cut(s) 884
PdmI GAANNNNTTC 1 cut(s) 1250
PfeI GAWTC 4 cut(s) 773, 1112, 1197, 1219
PflMI CCANNNNNTGG 1 cut(s) 852
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 4 cut(s) 237, 441, 444, 765
PleI GAGTC 1 cut(s) 991
PluTI GGCGCC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 991
PsiI TTATAA 1 cut(s) 1076
Psp6I CCWGG 2 cut(s) 45, 782
PspFI CCCAGC 1 cut(s) 1361
PspGI CCWGG 2 cut(s) 45, 782
PspN4I GGNNCC 2 cut(s) 210, 262
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 4 cut(s) 49, 79, 260, 512
RsaI GTAC 3 cut(s) 184, 951, 1174
RsaNI GTAC 3 cut(s) 183, 950, 1173
RseI CAYNNNNRTG 1 cut(s) 997
SaqAI TTAA 7 cut(s) 447, 567, 579, 651, 830, 939, 1368
SatI GCNGC 4 cut(s) 236, 440, 443, 764
Sau3AI GATC 9 cut(s) 49, 79, 136, 260, 339, 512, 532, 757, 912
SchI GAGTC 1 cut(s) 992
ScrFI CCNGG 4 cut(s) 47, 311, 424, 784
SduI GDGCHC 1 cut(s) 1023
SfaNI GCATC 3 cut(s) 261, 355, 1127
SfoI GGCGCC 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 997
SmlI CTYRAG 2 cut(s) 980, 1148
SmoI CTYRAG 2 cut(s) 980, 1148
Sse9I AATT 7 cut(s) 294, 386, 710, 832, 1006, 1042, 1063
SsiI CCGC 7 cut(s) 90, 96, 233, 278, 443, 468, 763
SspDI GGCGCC 1 cut(s) 208
SspMI CTAG 7 cut(s) 56, 362, 590, 1127, 1157, 1193, 1229
StyD4I CCNGG 4 cut(s) 45, 309, 422, 782
StyI CCWWGG 2 cut(s) 851, 1192
TaaI ACNGT 2 cut(s) 612, 1319
TaiI ACGT 1 cut(s) 16
TaqI TCGA 3 cut(s) 135, 1026, 1181
TaqII GACCGA 1 cut(s) 1265
TasI AATT 7 cut(s) 294, 386, 710, 832, 1006, 1042, 1063
TatI WGTACW 1 cut(s) 1172
TauI GCSGC 2 cut(s) 445, 766
TfiI GAWTC 4 cut(s) 773, 1112, 1197, 1219
Tru1I TTAA 7 cut(s) 447, 567, 579, 651, 830, 939, 1368
Tru9I TTAA 7 cut(s) 447, 567, 579, 651, 830, 939, 1368
TscAI CASTG 1 cut(s) 723
TseFI GTSAC 3 cut(s) 410, 1211, 1277
TseI GCWGC 2 cut(s) 235, 439
Tsp45I GTSAC 3 cut(s) 410, 1211, 1277
TspDTI ATGAA 6 cut(s) 109, 186, 693, 765, 877, 912
TspRI CASTG 1 cut(s) 723
Van91I CCANNNNNTGG 1 cut(s) 852
VneI GTGCAC 1 cut(s) 1019
XapI RAATTY 3 cut(s) 386, 832, 1006
XbaI TCTAGA 1 cut(s) 1156
XceI RCATGY 1 cut(s) 40
XcmI CCANNNNNNNNNTGG 1 cut(s) 669
XmaJI CCTAGG 1 cut(s) 1192
XmnI GAANNNNTTC 1 cut(s) 1250
XspI CTAG 7 cut(s) 56, 362, 590, 1127, 1157, 1193, 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.