RLG00000022359

Truncated transcription factor CAULIFLOWER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001081
Physical Location & Seq
Reverse (-)
82820120 .. 82832525
12406 bp
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UTR
Exon/CDS
Intron
RLM00000022359

Sequence Viewer

Length: 798 bp
ATGGGTAGAGGGAAGGTGCAACTGAAGCGAATCGAGGACAAGATCAGGCGCCAAGTCACTTTCTCCAAGAGAAGGACTGGGCTGATGAAGAAGGCTCGCGAGCTCTCCGTGCTGTGCGGCGTCGAGGTTGGGCTCATCATCTTCTCCGCCAAAGGGAGGCTCTACGAGTTCTGTAGCGGCGATAGTTTAGGAAATGTTCTTGAGCGTTACCAGATACATAATGAGGAAGAAGTTGCTGCTCCCAAGAATGCCAAGAAACATCATTTGGAATTGACTGGTAATGGCACATGCGCTAAGAAATCCCTGAAAATGATTCTAAGTGAACTAGAAGCACAGAACATTGAAAATCTGGATGTGACAGAGCTCACTCAACTTGAGAAGCAACTGGATGCTATATTGAGACAAACAAGATCAAGAAAGACGCAGCTCATGACGGAAACAATAACAGCTCTCATTGAGAAGGGAAAGAAGCTGGGAGAAGAGAAGGATCTCATGGAAAAGGAGATTGCAGCACTGATGGTGGAGAAGGCGAACCAGCCGGAACAGCCAACTGCGATTGCTGCTGCTATGGAAGGGGAACGAGAAGGAGAAGCAGTTGAGCACAGCAGTTGTGCCCACACCAAACATCCCCTGGGACATATGCATGCGACGTGGCAGCAGCAGATACTTCCCACCTCAACTCGCAATGACACTTGTAGCTATAGTAGTAGTCTTGACAGACGAAAGGACAAACCGCACCGTTTCAGCCACAATTGTATTTTGTATGTAGAAGAGTATGTTTGTAAAATTGTAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

266

Amino Acids

30.04

Weight (kDa)

8.74

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.3e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 108 - 169 1.5e-10 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 48
AccII CGCG 1 cut(s) 99
AciI CCGC 4 cut(s) 117, 147, 177, 734
AclWI GGATC 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 44
AcyI GRCGYC 2 cut(s) 49, 120
AfiI CCNNNNNNNGG 3 cut(s) 72, 153, 156
AgsI TTSAA 1 cut(s) 344
AjiI CACGTC 1 cut(s) 651
AjnI CCWGG 1 cut(s) 630
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 6 cut(s) 103, 364, 427, 449, 472, 699
AluI AGCT 6 cut(s) 103, 364, 427, 449, 472, 699
Alw21I GWGCWC 3 cut(s) 105, 366, 603
Alw26I GTCTC 1 cut(s) 394
AlwI GGATC 1 cut(s) 495
ApeKI GCWGC 7 cut(s) 236, 424, 509, 560, 563, 655, 658
AspLEI GCGC 2 cut(s) 51, 293
BaeGI GKGCMC 1 cut(s) 616
BanI GGYRCC 1 cut(s) 48
BanII GRGCYC 3 cut(s) 105, 135, 366
Bbv12I GWGCWC 3 cut(s) 105, 366, 603
BbvI GCAGC 7 cut(s) 223, 436, 521, 547, 550, 667, 670
BccI CCATC 1 cut(s) 511
BciT130I CCWGG 1 cut(s) 632
BcoDI GTCTC 1 cut(s) 394
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 2 cut(s) 172, 700
BfoI RGCGCY 1 cut(s) 52
BisI GCNGC 9 cut(s) 118, 178, 237, 425, 510, 561, 564, 656, 659
BlsI GCNGC 9 cut(s) 119, 179, 238, 426, 511, 562, 565, 657, 660
Bme1390I CCNGG 1 cut(s) 632
BmgBI CACGTC 1 cut(s) 651
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 1 cut(s) 632
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 87
BpuEI CTTGAG 2 cut(s) 221, 395
BsaHI GRCGYC 2 cut(s) 49, 120
BsaJI CCNNGG 2 cut(s) 630, 631
BsaXI ACNNNNNCTCC 2 cut(s) 579, 609
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 153, 156
Bse1I ACTGG 3 cut(s) 82, 280, 390
Bse3DI GCAATG 1 cut(s) 691
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 2 cut(s) 630, 631
BseGI GGATG 3 cut(s) 358, 394, 625
BseLI CCNNNNNNNGG 3 cut(s) 72, 153, 156
BseMI GCAATG 1 cut(s) 691
BseNI ACTGG 3 cut(s) 82, 280, 390
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 7 cut(s) 223, 436, 521, 547, 550, 667, 670
BseYI CCCAGC 1 cut(s) 472
Bsh1236I CGCG 1 cut(s) 99
BshNI GGYRCC 1 cut(s) 48
BsiHKAI GWGCWC 3 cut(s) 105, 366, 603
BsiSI CCGG 1 cut(s) 539
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 3 cut(s) 72, 153, 156
BsmAI GTCTC 1 cut(s) 394
BsmFI GGGAC 1 cut(s) 648
BsmI GAATGC 1 cut(s) 253
Bsp1286I GDGCHC 5 cut(s) 105, 135, 366, 603, 616
Bsp143I GATC 3 cut(s) 42, 410, 487
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 4 cut(s) 117, 147, 177, 734
BspFNI CGCG 1 cut(s) 99
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 50
BspPI GGATC 1 cut(s) 495
BspT107I GGYRCC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 691
BsrI ACTGG 3 cut(s) 82, 280, 390
BssECI CCNNGG 2 cut(s) 630, 631
BssMI GATC 3 cut(s) 42, 410, 487
BssNI GRCGYC 2 cut(s) 49, 120
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 1 cut(s) 740
Bst6I CTCTTC 2 cut(s) 474, 765
BstACI GRCGYC 2 cut(s) 49, 120
BstC8I GCNNGC 3 cut(s) 97, 101, 645
BstDEI CTNAG 2 cut(s) 294, 317
BstF5I GGATG 3 cut(s) 358, 394, 625
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 2 cut(s) 51, 293
BstKTI GATC 3 cut(s) 45, 413, 490
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 3 cut(s) 42, 410, 487
BstMWI GCNNNNNNNGC 4 cut(s) 25, 109, 544, 560
BstNI CCWGG 1 cut(s) 632
BstNSI RCATGY 2 cut(s) 291, 647
BstSCI CCNGG 1 cut(s) 630
BstSFI CTRYAG 2 cut(s) 172, 700
BstSLI GKGCMC 1 cut(s) 616
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 7 cut(s) 223, 436, 521, 547, 550, 667, 670
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BtrI CACGTC 1 cut(s) 651
BtsCI GGATG 3 cut(s) 358, 394, 625
BtsIMutI CAGTG 1 cut(s) 512
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 3 cut(s) 97, 101, 645
CciI TCATGA 1 cut(s) 429
CfoI GCGC 2 cut(s) 51, 293
CseI GACGC 2 cut(s) 109, 430
CviAII CATG 4 cut(s) 288, 430, 493, 644
DdeI CTNAG 2 cut(s) 294, 317
DinI GGCGCC 1 cut(s) 50
DpnI GATC 3 cut(s) 44, 412, 489
DpnII GATC 3 cut(s) 42, 410, 487
Eam1104I CTCTTC 2 cut(s) 474, 765
EarI CTCTTC 2 cut(s) 474, 765
EciI GGCGGA 1 cut(s) 136
Ecl136II GAGCTC 2 cut(s) 103, 364
Eco24I GRGCYC 3 cut(s) 105, 135, 366
Eco53kI GAGCTC 2 cut(s) 103, 364
Eco57I CTGAAG 1 cut(s) 44
EcoICRI GAGCTC 2 cut(s) 103, 364
EcoRII CCWGG 1 cut(s) 630
EcoT22I ATGCAT 1 cut(s) 645
EcoT38I GRGCYC 3 cut(s) 105, 135, 366
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
FaeI CATG 4 cut(s) 291, 433, 496, 647
FaqI GGGAC 1 cut(s) 648
FatI CATG 4 cut(s) 287, 429, 492, 643
FauNDI CATATG 1 cut(s) 639
Fnu4HI GCNGC 9 cut(s) 118, 178, 237, 425, 510, 561, 564, 656, 659
FokI GGATG 3 cut(s) 365, 401, 612
FriOI GRGCYC 3 cut(s) 105, 135, 366
Fsp4HI GCNGC 9 cut(s) 118, 178, 237, 425, 510, 561, 564, 656, 659
FspBI CTAG 1 cut(s) 326
GlaI GCGC 2 cut(s) 50, 292
GluI GCNGC 9 cut(s) 118, 178, 237, 425, 510, 561, 564, 656, 659
GsaI CCCAGC 1 cut(s) 476
HaeII RGCGCY 1 cut(s) 52
HapII CCGG 1 cut(s) 539
HgaI GACGC 2 cut(s) 109, 430
HhaI GCGC 2 cut(s) 51, 293
Hin1I GRCGYC 2 cut(s) 49, 120
Hin1II CATG 4 cut(s) 291, 433, 496, 647
Hin6I GCGC 2 cut(s) 49, 291
HinP1I GCGC 2 cut(s) 49, 291
HinfI GANTC 2 cut(s) 30, 313
HpaII CCGG 1 cut(s) 539
Hpy166II GTNNAC 1 cut(s) 323
Hpy188III TCNNGA 6 cut(s) 98, 200, 350, 414, 430, 713
Hpy8I GTNNAC 1 cut(s) 323
Hpy99I CGWCG 2 cut(s) 125, 652
HpyAV CCTTC 8 cut(s) 7, 66, 85, 454, 478, 520, 566, 578
HpyCH4III ACNGT 1 cut(s) 740
HpyCH4IV ACGT 1 cut(s) 650
HpyCH4V TGCA 3 cut(s) 19, 509, 643
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 109, 544, 560
HpyF3I CTNAG 2 cut(s) 294, 317
HpySE526I ACGT 1 cut(s) 650
Hsp92I GRCGYC 2 cut(s) 49, 120
Hsp92II CATG 4 cut(s) 291, 433, 496, 647
HspAI GCGC 2 cut(s) 49, 291
KasI GGCGCC 1 cut(s) 48
Kzo9I GATC 3 cut(s) 42, 410, 487
LmnI GCTCC 1 cut(s) 244
Lsp1109I GCAGC 7 cut(s) 223, 436, 521, 547, 550, 667, 670
LweI GCATC 1 cut(s) 379
MaeI CTAG 1 cut(s) 326
MaeII ACGT 1 cut(s) 650
MaeIII GTNAC 3 cut(s) 55, 206, 355
MalI GATC 3 cut(s) 44, 412, 489
MboI GATC 3 cut(s) 42, 410, 487
MboII GAAGA 5 cut(s) 100, 133, 239, 491, 782
MfeI CAATTG 1 cut(s) 751
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 5 cut(s) 105, 135, 366, 603, 616
MluCI AATT 3 cut(s) 269, 751, 786
Mly113I GGCGCC 1 cut(s) 49
MnlI CCTC 5 cut(s) 28, 118, 150, 217, 685
Mph1103I ATGCAT 1 cut(s) 645
MslI CAYNNNNRTG 1 cut(s) 642
MspI CCGG 1 cut(s) 539
MspR9I CCNGG 1 cut(s) 632
MunI CAATTG 1 cut(s) 751
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 4 cut(s) 25, 109, 544, 560
NarI GGCGCC 1 cut(s) 49
NdeI CATATG 1 cut(s) 639
NdeII GATC 3 cut(s) 42, 410, 487
NlaIII CATG 4 cut(s) 291, 433, 496, 647
NlaIV GGNNCC 1 cut(s) 50
NmuCI GTSAC 2 cut(s) 55, 355
NruI TCGCGA 1 cut(s) 99
NsiI ATGCAT 1 cut(s) 645
NspI RCATGY 2 cut(s) 291, 647
PaeI GCATGC 1 cut(s) 647
PagI TCATGA 1 cut(s) 429
PasI CCCWGGG 1 cut(s) 631
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 2 cut(s) 30, 313
PkrI GCNGC 9 cut(s) 119, 179, 238, 426, 511, 562, 565, 657, 660
PluTI GGCGCC 1 cut(s) 52
Psp124BI GAGCTC 2 cut(s) 105, 366
Psp6I CCWGG 1 cut(s) 630
PspFI CCCAGC 1 cut(s) 472
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 1 cut(s) 50
PsuI RGATCY 1 cut(s) 487
RruI TCGCGA 1 cut(s) 99
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 2 cut(s) 105, 366
SatI GCNGC 9 cut(s) 118, 178, 237, 425, 510, 561, 564, 656, 659
Sau3AI GATC 3 cut(s) 42, 410, 487
ScrFI CCNGG 1 cut(s) 632
SduI GDGCHC 5 cut(s) 105, 135, 366, 603, 616
SfaNI GCATC 1 cut(s) 379
SfcI CTRYAG 2 cut(s) 172, 700
SfoI GGCGCC 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 200, 374
SmoI CTYRAG 2 cut(s) 200, 374
SphI GCATGC 1 cut(s) 647
Sse9I AATT 3 cut(s) 269, 751, 786
SsiI CCGC 4 cut(s) 117, 147, 177, 734
SspDI GGCGCC 1 cut(s) 48
SspMI CTAG 1 cut(s) 326
SstI GAGCTC 2 cut(s) 105, 366
StyD4I CCNGG 1 cut(s) 630
TaaI ACNGT 1 cut(s) 740
TaiI ACGT 1 cut(s) 653
TaqI TCGA 2 cut(s) 33, 123
TasI AATT 3 cut(s) 269, 751, 786
TauI GCSGC 2 cut(s) 120, 180
TfiI GAWTC 2 cut(s) 30, 313
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 2 cut(s) 55, 355
TseI GCWGC 7 cut(s) 236, 424, 509, 560, 563, 655, 658
Tsp45I GTSAC 2 cut(s) 55, 355
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 2 cut(s) 97, 449
TspRI CASTG 1 cut(s) 519
XceI RCATGY 2 cut(s) 291, 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XspI CTAG 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.