Rmu_sc0003558.1_g000006

Truncated transcription factor CAULIFLOWER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003558.1
OG0001081
Physical Location & Seq
Forward (+)
27622 .. 33777
6156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003558.1_g000006.1.cds

Sequence Viewer

Length: 1224 bp
atgacatcctccgctgcaccttcatcagtatccatgccatcagttcccactacacctatctatcattttgtcgctgtcaaactgaatgatgataattttgtgctttggtctcaccaaattgaatcctatcttcatggtaacaaactcttcaagtttgtaggtgggtccttcccttgtccagctacctcttcttctgatcatgagaaacataatttggaattgactggtaatggcacatgcgctaagaaatccctgaaaatgattcaaagtgaactagaagcacagaacattgaaaatctggatgtgacagagctcactcaacttgagaagcaactggatgctatattaaggcaaacaagatcaagaaagacgcagctcatgatggaaacaataacaactctcattgagaagggaaagaagctgggagaagagaaggatctcatggaaaaggagattgcagcactgatggtggagaaggcgaaccagcaggaacagccaactgcgattgctgctgctaatgataatcatgatgatgaggaaatggaaggagaacgagaaggagaagcagatgagcgcagcagttgtgcccacaccaaacatcccggaccggataaacccggaccgagagtaaacggtccggtttggccgggctttcggttcctggcaacagttgcagcagtgctgcattttgccgatgaggttgccatcaagtctgaaacagaagtactcaatctgtcaagcataaagtcagcaattgcaacagcaatgctttcaagttacaagtttcaacagcaatccaagtttcaagctggaattcaagaagtagagaggagattaccgttctcgacttccgaacggcgagcagagtctgctcgaggtcctcttcgaaatcggagtcagaattgggagccgaggagaggtctaccgcctacgtcgtcgctttggttgattagtggccggaactccggaagactggccttcgatcgagggctcaaggcggagaggcggactggtggagtagggagagttgagatcgagagtcctcttcgaaatcggagtcagaattgggagccgaggagaggtctaccgcctacgtcgtcgctttggttgattagtggccggaactccggaagactggccttcgatcgagggctcaaggcggagaggcggactggtggagtagggagagttgagatcgagagtacgacaagtcgacataactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

407

Amino Acids

45.25

Weight (kDa)

8.86

Isoelectric Point (pI)

57.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 922, 1084, 1213
AccIII TCCGGA 2 cut(s) 965, 1127
AciI CCGC 7 cut(s) 12, 926, 998, 1006, 1088, 1160, 1168
AclWI GGATC 1 cut(s) 444
AcoI YGGCCR 3 cut(s) 644, 955, 1117
AcsI RAATTY 1 cut(s) 813
AfaI GTAC 2 cut(s) 726, 1204
AfiI CCNNNNNNNGG 2 cut(s) 917, 1079
AgsI TTSAA 8 cut(s) 122, 151, 266, 293, 774, 788, 806, 818
AjnI CCWGG 1 cut(s) 660
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 182, 313, 376, 421, 809
AluI AGCT 5 cut(s) 182, 313, 376, 421, 809
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 444
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 1 cut(s) 873
Aor13HI TCCGGA 2 cut(s) 965, 1127
AoxI GGCC 5 cut(s) 644, 955, 975, 1117, 1137
ApeKI GCWGC 8 cut(s) 14, 373, 458, 509, 512, 576, 674, 682
ApoI RAATTY 1 cut(s) 813
ArsI GACNNNNNNTTYG 4 cut(s) 880, 912, 1042, 1074
AspLEI GCGC 2 cut(s) 242, 576
AspS9I GGNCC 5 cut(s) 165, 605, 620, 635, 878
AsuC2I CCSGG 3 cut(s) 603, 618, 648
AsuHPI GGTGA 1 cut(s) 104
AsuII TTCGAA 2 cut(s) 886, 1048
AvaI CYCGRG 1 cut(s) 873
AvaII GGWCC 5 cut(s) 165, 605, 620, 635, 878
BaeGI GKGCMC 1 cut(s) 589
BanII GRGCYC 3 cut(s) 315, 993, 1155
BbsI GAAGAC 2 cut(s) 976, 1138
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 7 cut(s) 385, 470, 496, 499, 588, 669, 686
BccI CCATC 4 cut(s) 46, 376, 460, 713
BceAI ACGGC 1 cut(s) 872
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciT130I CCWGG 1 cut(s) 662
BciVI GTATCC 1 cut(s) 40
BclI TGATCA 1 cut(s) 196
BcnI CCSGG 3 cut(s) 603, 618, 648
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 275
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 8 cut(s) 15, 374, 459, 510, 513, 577, 675, 683
BlsI GCNGC 8 cut(s) 16, 375, 460, 511, 514, 578, 676, 684
BmcAI AGTACT 1 cut(s) 726
Bme1390I CCNGG 4 cut(s) 603, 618, 648, 662
Bme18I GGWCC 5 cut(s) 165, 605, 620, 635, 878
BmeT110I CYCGRG 1 cut(s) 873
BmgT120I GGNCC 5 cut(s) 165, 605, 620, 635, 878
BmiI GGNNCC 4 cut(s) 166, 659, 909, 1071
BmrFI CCNGG 4 cut(s) 603, 618, 648, 662
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 2 cut(s) 976, 1138
Bpu14I TTCGAA 2 cut(s) 886, 1048
BpuEI CTTGAG 3 cut(s) 344, 977, 1139
BpuMI CCSGG 3 cut(s) 603, 618, 648
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 2 cut(s) 911, 1073
BsaWI WCCGGW 4 cut(s) 607, 637, 965, 1127
Bsc4I CCNNNNNNNGG 2 cut(s) 917, 1079
Bse1I ACTGG 6 cut(s) 229, 339, 978, 1015, 1140, 1177
Bse3DI GCAATG 1 cut(s) 771
BseAI TCCGGA 2 cut(s) 965, 1127
BseBI CCWGG 1 cut(s) 662
BseDI CCNNGG 2 cut(s) 911, 1073
BseGI GGATG 4 cut(s) 5, 307, 343, 598
BseLI CCNNNNNNNGG 2 cut(s) 917, 1079
BseMI GCAATG 1 cut(s) 771
BseNI ACTGG 6 cut(s) 229, 339, 978, 1015, 1140, 1177
BseRI GAGGAG 3 cut(s) 844, 928, 1090
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 7 cut(s) 385, 470, 496, 499, 588, 669, 686
BseYI CCCAGC 1 cut(s) 421
Bsh1285I CGRYCG 2 cut(s) 985, 1147
BshFI GGCC 5 cut(s) 646, 957, 977, 1119, 1139
BsiEI CGRYCG 2 cut(s) 985, 1147
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 873
BsiSI CCGG 9 cut(s) 603, 608, 618, 638, 647, 958, 966, 1120, 1128
BslI CCNNNNNNNGG 2 cut(s) 917, 1079
BsmAI GTCTC 1 cut(s) 114
BsnI GGCC 5 cut(s) 646, 957, 977, 1119, 1139
Bso31I GGTCTC 1 cut(s) 114
BsoBI CYCGRG 1 cut(s) 873
Bsp119I TTCGAA 2 cut(s) 886, 1048
Bsp1286I GDGCHC 4 cut(s) 315, 589, 993, 1155
Bsp13I TCCGGA 2 cut(s) 965, 1127
Bsp143I GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
BspACI CCGC 7 cut(s) 12, 926, 998, 1006, 1088, 1160, 1168
BspANI GGCC 5 cut(s) 646, 957, 977, 1119, 1139
BspEI TCCGGA 2 cut(s) 965, 1127
BspHI TCATGA 3 cut(s) 199, 378, 526
BspLI GGNNCC 4 cut(s) 166, 659, 909, 1071
BspPI GGATC 1 cut(s) 444
BspT104I TTCGAA 2 cut(s) 886, 1048
BspTNI GGTCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 771
BsrI ACTGG 6 cut(s) 229, 339, 978, 1015, 1140, 1177
BssECI CCNNGG 2 cut(s) 911, 1073
BssMI GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
Bst2UI CCWGG 1 cut(s) 662
Bst4CI ACNGT 3 cut(s) 635, 670, 840
Bst6I CTCTTC 5 cut(s) 152, 193, 423, 888, 1050
BstAPI GCANNNNNTGC 2 cut(s) 671, 869
BstBI TTCGAA 2 cut(s) 886, 1048
BstC8I GCNNGC 1 cut(s) 861
BstDEI CTNAG 1 cut(s) 243
BstF5I GGATG 4 cut(s) 5, 307, 343, 598
BstHHI GCGC 2 cut(s) 242, 576
BstKTI GATC 7 cut(s) 199, 362, 439, 985, 1035, 1147, 1197
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
BstMCI CGRYCG 2 cut(s) 985, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 493, 509, 671, 869
BstNI CCWGG 1 cut(s) 662
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 4 cut(s) 601, 616, 646, 660
BstSLI GKGCMC 1 cut(s) 589
BstV1I GCAGC 7 cut(s) 385, 470, 496, 499, 588, 669, 686
BstV2I GAAGAC 2 cut(s) 976, 1138
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 5 cut(s) 646, 957, 977, 1119, 1139
BtsCI GGATG 4 cut(s) 5, 307, 343, 598
BtsI GCAGTG 1 cut(s) 684
BtsIMutI CAGTG 2 cut(s) 461, 684
Cac8I GCNNGC 1 cut(s) 861
CaiI CAGNNNCTG 1 cut(s) 869
CciI TCATGA 3 cut(s) 199, 378, 526
CfoI GCGC 2 cut(s) 242, 576
Cfr13I GGNCC 5 cut(s) 165, 605, 620, 635, 878
CpoI CGGWCCG 3 cut(s) 605, 620, 635
CseI GACGC 1 cut(s) 379
Csp6I GTAC 2 cut(s) 725, 1203
CspI CGGWCCG 3 cut(s) 605, 620, 635
CviAII CATG 7 cut(s) 34, 134, 200, 237, 379, 442, 527
CviQI GTAC 2 cut(s) 725, 1203
DdeI CTNAG 1 cut(s) 243
DpnI GATC 7 cut(s) 198, 361, 438, 984, 1034, 1146, 1196
DpnII GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
EaeI YGGCCR 3 cut(s) 644, 955, 1117
Eam1104I CTCTTC 5 cut(s) 152, 193, 423, 888, 1050
EarI CTCTTC 5 cut(s) 152, 193, 423, 888, 1050
EciI GGCGGA 4 cut(s) 1013, 1021, 1175, 1183
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 3 cut(s) 315, 993, 1155
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 5 cut(s) 165, 605, 620, 635, 878
Eco53kI GAGCTC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 873
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 2 cut(s) 165, 878
EcoRI GAATTC 1 cut(s) 813
EcoRII CCWGG 1 cut(s) 660
EcoT38I GRGCYC 3 cut(s) 315, 993, 1155
FaeI CATG 7 cut(s) 37, 137, 203, 240, 382, 445, 530
FatI CATG 7 cut(s) 33, 133, 199, 236, 378, 441, 526
FbaI TGATCA 1 cut(s) 196
FblI GTMKAC 3 cut(s) 922, 1084, 1213
Fnu4HI GCNGC 8 cut(s) 15, 374, 459, 510, 513, 577, 675, 683
FokI GGATG 3 cut(s) 314, 350, 585
FriOI GRGCYC 3 cut(s) 315, 993, 1155
Fsp4HI GCNGC 8 cut(s) 15, 374, 459, 510, 513, 577, 675, 683
FspBI CTAG 1 cut(s) 275
GlaI GCGC 2 cut(s) 241, 575
GluI GCNGC 8 cut(s) 15, 374, 459, 510, 513, 577, 675, 683
GsaI CCCAGC 1 cut(s) 425
HaeIII GGCC 5 cut(s) 646, 957, 977, 1119, 1139
HapII CCGG 9 cut(s) 603, 608, 618, 638, 647, 958, 966, 1120, 1128
HgaI GACGC 1 cut(s) 379
HhaI GCGC 2 cut(s) 242, 576
Hin1II CATG 7 cut(s) 37, 137, 203, 240, 382, 445, 530
Hin6I GCGC 2 cut(s) 240, 574
HinP1I GCGC 2 cut(s) 240, 574
HincII GTYRAC 1 cut(s) 1214
HindII GTYRAC 1 cut(s) 1214
HinfI GANTC 6 cut(s) 122, 262, 866, 895, 1039, 1057
HpaII CCGG 9 cut(s) 603, 608, 618, 638, 647, 958, 966, 1120, 1128
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 5 cut(s) 272, 631, 923, 1085, 1214
Hpy188I TCNGA 7 cut(s) 196, 715, 853, 894, 900, 1056, 1062
Hpy8I GTNNAC 5 cut(s) 272, 631, 923, 1085, 1214
Hpy99I CGWCG 4 cut(s) 937, 940, 1099, 1102
HpyAV CCTTC 9 cut(s) 30, 178, 403, 427, 469, 539, 551, 988, 1150
HpyCH4III ACNGT 3 cut(s) 635, 670, 840
HpyCH4IV ACGT 2 cut(s) 932, 1094
HpyCH4V TGCA 5 cut(s) 17, 458, 674, 685, 758
HpyF10VI GCNNNNNNNGC 4 cut(s) 493, 509, 671, 869
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 2 cut(s) 932, 1094
Hsp92II CATG 7 cut(s) 37, 137, 203, 240, 382, 445, 530
HspAI GCGC 2 cut(s) 240, 574
Kpn2I TCCGGA 2 cut(s) 965, 1127
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
LmnI GCTCC 2 cut(s) 907, 1069
Lsp1109I GCAGC 7 cut(s) 385, 470, 496, 499, 588, 669, 686
LweI GCATC 1 cut(s) 328
MaeI CTAG 1 cut(s) 275
MaeII ACGT 2 cut(s) 932, 1094
MaeIII GTNAC 3 cut(s) 137, 304, 776
MalI GATC 7 cut(s) 198, 361, 438, 984, 1034, 1146, 1196
MboI GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
MboII GAAGA 9 cut(s) 122, 139, 180, 183, 440, 875, 981, 1037, 1143
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 4 cut(s) 315, 589, 993, 1155
MluCI AATT 8 cut(s) 94, 117, 211, 218, 753, 813, 901, 1063
MlyI GAGTC 4 cut(s) 875, 904, 1048, 1066
MroI TCCGGA 2 cut(s) 965, 1127
MseI TTAA 1 cut(s) 347
MslI CAYNNNNRTG 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 9 cut(s) 603, 608, 618, 638, 647, 958, 966, 1120, 1128
MspR9I CCNGG 4 cut(s) 603, 618, 648, 662
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 1 cut(s) 662
MwoI GCNNNNNNNGC 4 cut(s) 493, 509, 671, 869
NciI CCSGG 3 cut(s) 603, 618, 648
NdeII GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
NlaIII CATG 7 cut(s) 37, 137, 203, 240, 382, 445, 530
NlaIV GGNNCC 4 cut(s) 166, 659, 909, 1071
NmeAIII GCCGAG 2 cut(s) 936, 1098
NmuCI GTSAC 1 cut(s) 304
NspI RCATGY 1 cut(s) 240
NspV TTCGAA 2 cut(s) 886, 1048
PaeR7I CTCGAG 1 cut(s) 873
PagI TCATGA 3 cut(s) 199, 378, 526
PfeI GAWTC 2 cut(s) 122, 262
PfoI TCCNGGA 1 cut(s) 601
PkrI GCNGC 8 cut(s) 16, 375, 460, 511, 514, 578, 676, 684
Ple19I CGATCG 2 cut(s) 985, 1147
PleI GAGTC 4 cut(s) 874, 903, 1047, 1065
PpsI GAGTC 4 cut(s) 874, 903, 1047, 1065
PpuMI RGGWCCY 2 cut(s) 165, 878
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 2 cut(s) 165, 878
Psp6I CCWGG 1 cut(s) 660
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 1 cut(s) 660
PspN4I GGNNCC 4 cut(s) 166, 659, 909, 1071
PspPI GGNCC 5 cut(s) 165, 605, 620, 635, 878
PspPPI RGGWCCY 2 cut(s) 165, 878
PspXI VCTCGAGB 1 cut(s) 873
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 1 cut(s) 436
PvuI CGATCG 2 cut(s) 985, 1147
RsaI GTAC 2 cut(s) 726, 1204
RsaNI GTAC 2 cut(s) 725, 1203
RseI CAYNNNNRTG 1 cut(s) 531
Rsr2I CGGWCCG 3 cut(s) 605, 620, 635
RsrII CGGWCCG 3 cut(s) 605, 620, 635
SacI GAGCTC 1 cut(s) 315
SalI GTCGAC 1 cut(s) 1212
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 8 cut(s) 15, 374, 459, 510, 513, 577, 675, 683
Sau3AI GATC 7 cut(s) 196, 359, 436, 982, 1032, 1144, 1194
Sau96I GGNCC 5 cut(s) 165, 605, 620, 635, 878
ScaI AGTACT 1 cut(s) 726
SchI GAGTC 4 cut(s) 875, 904, 1048, 1066
ScrFI CCNGG 4 cut(s) 603, 618, 648, 662
SduI GDGCHC 4 cut(s) 315, 589, 993, 1155
SfaNI GCATC 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 873
SfuI TTCGAA 2 cut(s) 886, 1048
SinI GGWCC 5 cut(s) 165, 605, 620, 635, 878
SlaI CTCGAG 1 cut(s) 873
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 4 cut(s) 323, 873, 992, 1154
SmoI CTYRAG 4 cut(s) 323, 873, 992, 1154
Sse9I AATT 8 cut(s) 94, 117, 211, 218, 753, 813, 901, 1063
SsiI CCGC 7 cut(s) 12, 926, 998, 1006, 1088, 1160, 1168
SspMI CTAG 1 cut(s) 275
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 4 cut(s) 601, 616, 646, 660
TaaI ACNGT 3 cut(s) 635, 670, 840
TaiI ACGT 2 cut(s) 935, 1097
TaqII GACCGA 1 cut(s) 637
TasI AATT 8 cut(s) 94, 117, 211, 218, 753, 813, 901, 1063
TatI WGTACW 1 cut(s) 724
TfiI GAWTC 2 cut(s) 122, 262
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TscAI CASTG 2 cut(s) 468, 684
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 8 cut(s) 14, 373, 458, 509, 512, 576, 674, 682
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 12, 122
TspRI CASTG 2 cut(s) 468, 684
VpaK11BI GGWCC 5 cut(s) 165, 605, 620, 635, 878
XapI RAATTY 1 cut(s) 813
XceI RCATGY 1 cut(s) 240
XhoI CTCGAG 1 cut(s) 873
XmiI GTMKAC 3 cut(s) 922, 1084, 1213
XspI CTAG 1 cut(s) 275
ZrmI AGTACT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.