RLG00000024994

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008181
Physical Location & Seq
Forward (+)
41205843 .. 41206716
874 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024994

Sequence Viewer

Length: 765 bp
ATGGGTGTGCGTTGCTGCAACTTCCTCCATCTCCGATGGAGCAAATTTCTGGCACTTCCCCTAGTGTCGCAGCCTTCGAGCATGGTCCAGGCCGTCTCTTCCTTTTACCCGTTACTCTCCAAGACGTCGAGGTCTTCGATGCGTGTTGAAGCGACGTTGTTCATCCATCCTCAGGTTGGTTCGATCTGGGTCTTGAGGTCGCCTTCGGTTCATCATCCATTTGGATTGGGGGCTATTGGTGACAGGAAAATCTGGGATCCAAGGCGTAGTCGGTGTTCGATCCATCCTTCCGGGGCGCTGAAGAGGCGTTGGGAGCGGTGCAGGGATCGGGTCCTGGGCAACATCTGTACTGCGGTGCTGGGTCATCTCCGTCCAAGAAGGGCACGGCTGAAACTGGATATAAAAGGTGCTGGGTCTGGTGTTGATGGCGCTTGGTGCGTCCCAGATCTAGGCAGAATGGGTTCGGATTGCTTCCGGCGAATGATGGACGCGCGACTGTTAGAAGGGTGGTGCAGCAGTGGTCGGGGCCGAGGTCAGGGAGGCGAAGATCTGAATGGAGCAGCGTTTGGGCTTCGGGCTAGAACTGCGATACATCTGGTAGAGGCGGCTGGAGTCGGGGTGGTGGGACCGGACTGTGCTTTCTCCGGCGACGGGCAATGGTGCAGCTGTCGGGGCCGACAGTACGGCTGGTGCCGAGAAAGGGTGTGGTGCCCAAAGCAGATCCTTGTGGCCTTGGGCCTCTACTCTGTTGGCTTGCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.02

Weight (kDa)

10.27

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 130
AatII GACGTC 1 cut(s) 128
AccB1I GGYRCC 2 cut(s) 690, 708
AccBSI CCGCTC 1 cut(s) 316
AccII CGCG 2 cut(s) 491, 493
AciI CCGC 3 cut(s) 316, 353, 605
AclWI GGATC 5 cut(s) 251, 264, 274, 333, 715
AcsI RAATTY 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 2 cut(s) 349, 683
AfiI CCNNNNNNNGG 6 cut(s) 172, 176, 449, 535, 651, 700
AgsI TTSAA 1 cut(s) 149
AjnI CCWGG 2 cut(s) 87, 333
AluBI AGCT 1 cut(s) 666
AluI AGCT 1 cut(s) 666
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 5 cut(s) 251, 264, 274, 333, 715
AoxI GGCC 5 cut(s) 90, 526, 673, 729, 736
ApeKI GCWGC 5 cut(s) 15, 70, 513, 560, 663
ApoI RAATTY 1 cut(s) 44
Asp700I GAANNNNTTC 1 cut(s) 460
AspLEI GCGC 3 cut(s) 298, 431, 493
AspS9I GGNCC 6 cut(s) 85, 331, 526, 626, 673, 736
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 3 cut(s) 85, 331, 626
AxyI CCTNAGG 1 cut(s) 171
BaeGI GKGCMC 2 cut(s) 385, 713
BamHI GGATCC 1 cut(s) 256
BanI GGYRCC 2 cut(s) 690, 708
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 5 cut(s) 2, 82, 525, 572, 675
BccI CCATC 6 cut(s) 30, 36, 174, 291, 419, 478
BceAI ACGGC 3 cut(s) 77, 401, 700
BciT130I CCWGG 2 cut(s) 89, 335
BcnI CCSGG 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 3 cut(s) 62, 449, 579
BfoI RGCGCY 2 cut(s) 299, 432
BglII AGATCT 2 cut(s) 445, 547
BisI GCNGC 6 cut(s) 16, 71, 514, 561, 606, 664
BlsI GCNGC 6 cut(s) 17, 72, 515, 562, 607, 665
Bme1390I CCNGG 3 cut(s) 89, 292, 335
Bme18I GGWCC 3 cut(s) 85, 331, 626
BmgT120I GGNCC 6 cut(s) 85, 331, 526, 626, 673, 736
BmiI GGNNCC 7 cut(s) 258, 332, 527, 627, 674, 692, 710
BmrFI CCNGG 3 cut(s) 89, 292, 335
BmsI GCATC 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 214, 743
BpuMI CCSGG 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 5 cut(s) 260, 291, 334, 529, 732
BsaWI WCCGGW 1 cut(s) 628
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 6 cut(s) 172, 176, 449, 535, 651, 700
Bse1I ACTGG 1 cut(s) 399
Bse21I CCTNAGG 1 cut(s) 171
Bse3DI GCAATG 1 cut(s) 662
BseBI CCWGG 2 cut(s) 89, 335
BseDI CCNNGG 5 cut(s) 260, 291, 334, 529, 732
BseGI GGATG 4 cut(s) 162, 166, 214, 283
BseLI CCNNNNNNNGG 6 cut(s) 172, 176, 449, 535, 651, 700
BseMI GCAATG 1 cut(s) 662
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 1 cut(s) 399
BseSI GKGCMC 2 cut(s) 385, 713
BseXI GCAGC 5 cut(s) 2, 82, 525, 572, 675
BseYI CCCAGC 2 cut(s) 358, 410
BsgI GTGCAG 3 cut(s) 340, 532, 682
Bsh1236I CGCG 2 cut(s) 491, 493
BshFI GGCC 5 cut(s) 92, 528, 675, 731, 738
BshNI GGYRCC 2 cut(s) 690, 708
BsiSI CCGG 4 cut(s) 291, 475, 629, 645
BslFI GGGAC 2 cut(s) 425, 639
BslI CCNNNNNNNGG 6 cut(s) 172, 176, 449, 535, 651, 700
BsmAI GTCTC 1 cut(s) 100
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 2 cut(s) 425, 639
BsnI GGCC 5 cut(s) 92, 528, 675, 731, 738
Bsp1286I GDGCHC 2 cut(s) 385, 713
Bsp143I GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
BspACI CCGC 3 cut(s) 316, 353, 605
BspANI GGCC 5 cut(s) 92, 528, 675, 731, 738
BspCNI CTCAG 1 cut(s) 184
BspFNI CGCG 2 cut(s) 491, 493
BspLI GGNNCC 7 cut(s) 258, 332, 527, 627, 674, 692, 710
BspPI GGATC 5 cut(s) 251, 264, 274, 333, 715
BspT107I GGYRCC 2 cut(s) 690, 708
BsrBI CCGCTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 662
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 5 cut(s) 260, 291, 334, 529, 732
BssMI GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 2 cut(s) 260, 732
Bst2UI CCWGG 2 cut(s) 89, 335
Bst4CI ACNGT 3 cut(s) 498, 635, 681
Bst6I CTCTTC 2 cut(s) 103, 296
BstACI GRCGYC 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 755
BstDEI CTNAG 1 cut(s) 171
BstF5I GGATG 4 cut(s) 162, 166, 214, 283
BstFNI CGCG 2 cut(s) 491, 493
BstH2I RGCGCY 2 cut(s) 299, 432
BstHHI GCGC 3 cut(s) 298, 431, 493
BstKTI GATC 7 cut(s) 186, 259, 282, 328, 448, 550, 723
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
BstMWI GCNNNNNNNGC 5 cut(s) 304, 313, 435, 584, 672
BstNI CCWGG 2 cut(s) 89, 335
BstSCI CCNGG 3 cut(s) 87, 290, 333
BstSLI GKGCMC 2 cut(s) 385, 713
BstUI CGCG 2 cut(s) 491, 493
BstV1I GCAGC 5 cut(s) 2, 82, 525, 572, 675
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 4 cut(s) 256, 445, 547, 720
BstYI RGATCY 4 cut(s) 256, 445, 547, 720
Bsu36I CCTNAGG 1 cut(s) 171
BsuRI GGCC 5 cut(s) 92, 528, 675, 731, 738
BtsCI GGATG 4 cut(s) 162, 166, 214, 283
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 1 cut(s) 523
Cac8I GCNNGC 1 cut(s) 755
CfoI GCGC 3 cut(s) 298, 431, 493
Cfr13I GGNCC 6 cut(s) 85, 331, 526, 626, 673, 736
CseI GACGC 2 cut(s) 427, 497
Csp6I GTAC 2 cut(s) 348, 682
CviAII CATG 1 cut(s) 82
CviQI GTAC 2 cut(s) 348, 682
DdeI CTNAG 1 cut(s) 171
DpnI GATC 7 cut(s) 185, 258, 281, 327, 447, 549, 722
DpnII GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 103, 296
EarI CTCTTC 2 cut(s) 103, 296
Eco130I CCWWGG 2 cut(s) 260, 732
Eco47I GGWCC 3 cut(s) 85, 331, 626
Eco57I CTGAAG 1 cut(s) 320
Eco81I CCTNAGG 1 cut(s) 171
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 87, 333
EcoT14I CCWWGG 2 cut(s) 260, 732
ErhI CCWWGG 2 cut(s) 260, 732
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 1 cut(s) 85
FaiI YATR 2 cut(s) 83, 401
FaqI GGGAC 2 cut(s) 425, 639
FatI CATG 1 cut(s) 81
Fnu4HI GCNGC 6 cut(s) 16, 71, 514, 561, 606, 664
FokI GGATG 4 cut(s) 149, 153, 201, 270
Fsp4HI GCNGC 6 cut(s) 16, 71, 514, 561, 606, 664
FspBI CTAG 3 cut(s) 62, 449, 579
GlaI GCGC 3 cut(s) 297, 430, 492
GluI GCNGC 6 cut(s) 16, 71, 514, 561, 606, 664
GsaI CCCAGC 2 cut(s) 362, 414
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 2 cut(s) 299, 432
HaeIII GGCC 5 cut(s) 92, 528, 675, 731, 738
HapII CCGG 4 cut(s) 291, 475, 629, 645
HgaI GACGC 2 cut(s) 427, 497
HhaI GCGC 3 cut(s) 298, 431, 493
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 1 cut(s) 85
Hin6I GCGC 3 cut(s) 296, 429, 491
HinP1I GCGC 3 cut(s) 296, 429, 491
HinfI GANTC 1 cut(s) 612
HpaII CCGG 4 cut(s) 291, 475, 629, 645
HphI GGTGA 1 cut(s) 251
Hpy188I TCNGA 3 cut(s) 35, 466, 552
Hpy188III TCNNGA 1 cut(s) 193
Hpy99I CGWCG 3 cut(s) 130, 157, 653
HpyAV CCTTC 5 cut(s) 84, 213, 297, 372, 497
HpyCH4III ACNGT 3 cut(s) 498, 635, 681
HpyCH4IV ACGT 2 cut(s) 125, 155
HpyCH4V TGCA 4 cut(s) 18, 321, 513, 663
HpyF10VI GCNNNNNNNGC 5 cut(s) 304, 313, 435, 584, 672
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 2 cut(s) 125, 155
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 1 cut(s) 85
HspAI GCGC 3 cut(s) 296, 429, 491
Kzo9I GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
LmnI GCTCC 3 cut(s) 39, 313, 557
Lsp1109I GCAGC 5 cut(s) 2, 82, 525, 572, 675
LweI GCATC 1 cut(s) 129
MaeI CTAG 3 cut(s) 62, 449, 579
MaeII ACGT 2 cut(s) 125, 155
MaeIII GTNAC 2 cut(s) 111, 239
MalI GATC 7 cut(s) 185, 258, 281, 327, 447, 549, 722
MbiI CCGCTC 1 cut(s) 316
MboI GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
MboII GAAGA 4 cut(s) 90, 126, 313, 557
MflI RGATCY 4 cut(s) 256, 445, 547, 720
MhlI GDGCHC 2 cut(s) 385, 713
MluCI AATT 1 cut(s) 44
MlyI GAGTC 1 cut(s) 621
MnlI CCTC 9 cut(s) 35, 123, 180, 189, 297, 524, 533, 595, 749
MroXI GAANNNNTTC 1 cut(s) 460
MspA1I CMGCKG 1 cut(s) 666
MspI CCGG 4 cut(s) 291, 475, 629, 645
MspR9I CCNGG 3 cut(s) 89, 292, 335
MvaI CCWGG 2 cut(s) 89, 335
MvnI CGCG 2 cut(s) 491, 493
MwoI GCNNNNNNNGC 5 cut(s) 304, 313, 435, 584, 672
NciI CCSGG 1 cut(s) 292
NdeII GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
NlaIII CATG 1 cut(s) 85
NlaIV GGNNCC 7 cut(s) 258, 332, 527, 627, 674, 692, 710
NmeAIII GCCGAG 2 cut(s) 554, 719
NmuCI GTSAC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 134
PdmI GAANNNNTTC 1 cut(s) 460
PkrI GCNGC 6 cut(s) 17, 72, 515, 562, 607, 665
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
PpuMI RGGWCCY 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 87, 333
PspFI CCCAGC 2 cut(s) 358, 410
PspGI CCWGG 2 cut(s) 87, 333
PspN4I GGNNCC 7 cut(s) 258, 332, 527, 627, 674, 692, 710
PspPI GGNCC 6 cut(s) 85, 331, 526, 626, 673, 736
PspPPI RGGWCCY 1 cut(s) 331
PsrI GAACNNNNNNTAC 2 cut(s) 259, 291
PsuI RGATCY 4 cut(s) 256, 445, 547, 720
PvuII CAGCTG 1 cut(s) 666
RsaI GTAC 2 cut(s) 349, 683
RsaNI GTAC 2 cut(s) 348, 682
SatI GCNGC 6 cut(s) 16, 71, 514, 561, 606, 664
Sau3AI GATC 7 cut(s) 183, 256, 279, 325, 445, 547, 720
Sau96I GGNCC 6 cut(s) 85, 331, 526, 626, 673, 736
SchI GAGTC 1 cut(s) 621
ScrFI CCNGG 3 cut(s) 89, 292, 335
SduI GDGCHC 2 cut(s) 385, 713
SetI ASST 8 cut(s) 128, 134, 158, 177, 200, 409, 535, 668
SfaNI GCATC 1 cut(s) 129
SinI GGWCC 3 cut(s) 85, 331, 626
SmlI CTYRAG 2 cut(s) 193, 758
SmoI CTYRAG 2 cut(s) 193, 758
Sse9I AATT 1 cut(s) 44
SsiI CCGC 3 cut(s) 316, 353, 605
SspMI CTAG 3 cut(s) 62, 449, 579
StyD4I CCNGG 3 cut(s) 87, 290, 333
StyI CCWWGG 2 cut(s) 260, 732
TaaI ACNGT 3 cut(s) 498, 635, 681
TaiI ACGT 2 cut(s) 128, 158
TaqI TCGA 5 cut(s) 77, 128, 137, 182, 278
TasI AATT 1 cut(s) 44
TatI WGTACW 1 cut(s) 347
TauI GCSGC 1 cut(s) 608
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 5 cut(s) 15, 70, 513, 560, 663
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 151, 200
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 523
VpaK11BI GGWCC 3 cut(s) 85, 331, 626
XapI RAATTY 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 3 cut(s) 62, 449, 579
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.