Rroxscaffold_5G00353760

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008181
Physical Location & Seq
Forward (+)
31677772 .. 31686698
8927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353760.1

Sequence Viewer

Length: 1044 bp
ATGGATGGAGGTTGTGCTAACGGTGGCTTTTCATATGGAAATTTTAAAAACCCTAAGCGCCATTACTACTCGGAAAACCCTAGATTGAAAGATGGCTTTTTCCAGTCTAATTCCATCGCGAAACTGCACCAGATGATGTCCATCGATGAAGACACTATATCCTTTGTTGCTTCGTTGGCTCTTGTCGGCAACAAAGTCATCGATATCTCAAGATTGGCAAGTGGATCTTCACAGATGTTGATGGGAGCACTTTGGCCTTTCAAGATAAGTGAGGATCTAGACAGTTGGGTTGAACAAGTAGCCAAAGTTCCCACATTTCACGTACAATCACTGCATTGTGGTTATCGCTTTCGTAGTTTTATACCCTTAGGGGACTCTAGCTCGCAAGAGGGCTTGGCACTCATGTCAAACCCTGCAAGATCTGTGGTTGCAGACACATGGTGCACAACGCCAACAATTTTTGCTGTAATGGGCATTGCATGGAAATCAGTCTACTATTCTGGGCTCCCAACTAGTGATGGATTTGGTGGGGAGAGGGCGCATCACATCTTATGTATTCTGCGCGGAAAAGTATTTGTAAAACGGCCTCAAAAAAGCTGTAAGAGAATATATGGCTGGTGGAGTCATGTTGGTGGGAGCGGGGTGTGCCTGCTTCGAAGACGGACGGTGGAGCAGCGGTCGAGCCACGATCAAGGTGGGTGCCCAGAGAGGCTTTGGGTGCCCAAAGTAGATCCTGGTGGGCCTAGGCTTCTATTCTCGTTGGGTTGCCCAACTAGGGCTTTGGGCTGGGTCTTCTTTATTGGGGCTTGGCAGGTGGTGAATGAGCCCGAAGCTTTTAGGGTTCGCATTTGGGATCTGGGAGATTTCTTTTGGACCCTAAATTTATCTGCAATTACTTCTTTGAAAGAAAAGATCTTTGGATCATCTGAGATCATCACCATGGTGAAGCCACAGGGCAGAAAAAGCAGACATGAGATCAAAGAGCGTCGACCTAATTTCACAAGTCCAAAATCAACCAATAAGAGGCCCAAGAGGAATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

347

Amino Acids

38.97

Weight (kDa)

9.69

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 802
Acc36I ACCTGC 1 cut(s) 802
AccB1I GGYRCC 2 cut(s) 699, 718
AccBSI CCGCTC 1 cut(s) 639
AccI GTMKAC 2 cut(s) 492, 988
AccII CGCG 2 cut(s) 119, 564
AciI CCGC 3 cut(s) 564, 639, 676
AclWI GGATC 5 cut(s) 232, 282, 725, 861, 928
AcsI RAATTY 2 cut(s) 40, 880
AdeI CACNNNGTG 1 cut(s) 441
AfaI GTAC 1 cut(s) 324
AfiI CCNNNNNNNGG 2 cut(s) 775, 1023
AgsI TTSAA 4 cut(s) 88, 262, 293, 904
AhlI ACTAGT 1 cut(s) 512
AjnI CCWGG 1 cut(s) 733
AjuI GAANNNNNNNTTGG 2 cut(s) 900, 932
AleI CACNNNNGTG 1 cut(s) 941
AluBI AGCT 3 cut(s) 381, 597, 833
AluI AGCT 3 cut(s) 381, 597, 833
Alw21I GWGCWC 2 cut(s) 250, 446
Alw44I GTGCAC 1 cut(s) 442
AlwI GGATC 5 cut(s) 232, 282, 725, 861, 928
AoxI GGCC 4 cut(s) 254, 584, 740, 1025
ApaLI GTGCAC 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 673
ApoI RAATTY 2 cut(s) 40, 880
AspA2I CCTAGG 1 cut(s) 743
AspLEI GCGC 3 cut(s) 60, 541, 564
AspS9I GGNCC 3 cut(s) 740, 873, 1026
AsuHPI GGTGA 3 cut(s) 829, 928, 955
AsuII TTCGAA 1 cut(s) 655
AvaII GGWCC 1 cut(s) 873
AvrII CCTAGG 1 cut(s) 743
AxyI CCTNAGG 1 cut(s) 367
BaeGI GKGCMC 3 cut(s) 446, 704, 723
BanI GGYRCC 2 cut(s) 699, 718
BanII GRGCYC 2 cut(s) 507, 828
BbsI GAAGAC 3 cut(s) 156, 664, 784
Bbv12I GWGCWC 2 cut(s) 250, 446
BbvI GCAGC 1 cut(s) 685
BccI CCATC 5 cut(s) 86, 122, 149, 235, 512
BceAI ACGGC 1 cut(s) 599
BciT130I CCWGG 1 cut(s) 735
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 6 cut(s) 81, 278, 378, 513, 744, 774
BfoI RGCGCY 1 cut(s) 61
BfuAI ACCTGC 1 cut(s) 802
BglII AGATCT 2 cut(s) 419, 912
BisI GCNGC 1 cut(s) 674
BlnI CCTAGG 1 cut(s) 743
BlsI GCNGC 1 cut(s) 675
Bme1390I CCNGG 1 cut(s) 735
Bme18I GGWCC 1 cut(s) 873
BmgT120I GGNCC 3 cut(s) 740, 873, 1026
BmiI GGNNCC 4 cut(s) 506, 701, 720, 875
BmrFI CCNGG 1 cut(s) 735
BmsI GCATC 1 cut(s) 550
BpiI GAAGAC 3 cut(s) 156, 664, 784
Bpu10I CCTNAGC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 655
BpuEI CTTGAG 1 cut(s) 193
Bsa29I ATCGAT 2 cut(s) 144, 201
BsaAI YACGTR 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 140
BsaJI CCNNGG 2 cut(s) 743, 939
BsaXI ACNNNNNCTCC 2 cut(s) 662, 692
Bsc4I CCNNNNNNNGG 2 cut(s) 775, 1023
Bse1I ACTGG 1 cut(s) 103
Bse21I CCTNAGG 1 cut(s) 367
Bse3DI GCAATG 1 cut(s) 474
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 1 cut(s) 735
BseCI ATCGAT 2 cut(s) 144, 201
BseDI CCNNGG 2 cut(s) 743, 939
BseGI GGATG 1 cut(s) 10
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 775, 1023
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 1 cut(s) 918
BseNI ACTGG 1 cut(s) 103
BseSI GKGCMC 3 cut(s) 446, 704, 723
BseXI GCAGC 1 cut(s) 685
BseYI CCCAGC 1 cut(s) 786
BsgI GTGCAG 1 cut(s) 110
Bsh1236I CGCG 2 cut(s) 119, 564
Bsh1285I CGRYCG 1 cut(s) 680
BshFI GGCC 4 cut(s) 256, 586, 742, 1027
BshNI GGYRCC 2 cut(s) 699, 718
BshVI ATCGAT 2 cut(s) 144, 201
BsiEI CGRYCG 1 cut(s) 680
BsiHKAI GWGCWC 2 cut(s) 250, 446
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 2 cut(s) 775, 1023
BsmFI GGGAC 1 cut(s) 386
BsnI GGCC 4 cut(s) 256, 586, 742, 1027
Bsp119I TTCGAA 1 cut(s) 655
Bsp1286I GDGCHC 6 cut(s) 250, 446, 507, 704, 723, 828
Bsp19I CCATGG 1 cut(s) 939
Bsp68I TCGCGA 1 cut(s) 119
BspACI CCGC 3 cut(s) 564, 639, 676
BspANI GGCC 4 cut(s) 256, 586, 742, 1027
BspCNI CTCAG 1 cut(s) 919
BspDI ATCGAT 2 cut(s) 144, 201
BspFNI CGCG 2 cut(s) 119, 564
BspLI GGNNCC 4 cut(s) 506, 701, 720, 875
BspMI ACCTGC 1 cut(s) 802
BspPI GGATC 5 cut(s) 232, 282, 725, 861, 928
BspT104I TTCGAA 1 cut(s) 655
BspT107I GGYRCC 2 cut(s) 699, 718
BsrBI CCGCTC 1 cut(s) 639
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 2 cut(s) 743, 939
BssT1I CCWWGG 2 cut(s) 743, 939
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 3 cut(s) 23, 284, 667
BstBAI YACGTR 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 655
BstC8I GCNNGC 2 cut(s) 383, 650
BstDEI CTNAG 3 cut(s) 54, 367, 927
BstDSI CCRYGG 1 cut(s) 939
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 119, 564
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 3 cut(s) 60, 541, 564
BstMCI CGRYCG 1 cut(s) 680
BstMWI GCNNNNNNNGC 4 cut(s) 176, 645, 718, 963
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 1 cut(s) 733
BstSLI GKGCMC 3 cut(s) 446, 704, 723
BstUI CGCG 2 cut(s) 119, 564
BstV1I GCAGC 1 cut(s) 685
BstV2I GAAGAC 3 cut(s) 156, 664, 784
BstX2I RGATCY 6 cut(s) 224, 274, 419, 730, 853, 912
BstYI RGATCY 6 cut(s) 224, 274, 419, 730, 853, 912
Bsu15I ATCGAT 2 cut(s) 144, 201
Bsu36I CCTNAGG 1 cut(s) 367
BsuRI GGCC 4 cut(s) 256, 586, 742, 1027
BsuTUI ATCGAT 2 cut(s) 144, 201
BtgI CCRYGG 1 cut(s) 939
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 1 cut(s) 329
BtuMI TCGCGA 1 cut(s) 119
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 2 cut(s) 383, 650
CfoI GCGC 3 cut(s) 60, 541, 564
Cfr13I GGNCC 3 cut(s) 740, 873, 1026
ClaI ATCGAT 2 cut(s) 144, 201
CseI GACGC 1 cut(s) 974
Csp6I GTAC 1 cut(s) 323
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 6 cut(s) 403, 438, 480, 626, 940, 971
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 3 cut(s) 54, 367, 927
DraI TTTAAA 1 cut(s) 46
DraIII CACNNNGTG 1 cut(s) 441
Eco130I CCWWGG 2 cut(s) 743, 939
Eco24I GRGCYC 2 cut(s) 507, 828
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 873
Eco81I CCTNAGG 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 733
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 743, 939
EcoT38I GRGCYC 2 cut(s) 507, 828
ErhI CCWWGG 2 cut(s) 743, 939
FaeI CATG 6 cut(s) 406, 441, 483, 629, 943, 974
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FaqI GGGAC 1 cut(s) 386
FatI CATG 6 cut(s) 402, 437, 479, 625, 939, 970
FauI CCCGC 1 cut(s) 632
FauNDI CATATG 1 cut(s) 34
FblI GTMKAC 2 cut(s) 492, 988
Fnu4HI GCNGC 1 cut(s) 674
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 2 cut(s) 507, 828
Fsp4HI GCNGC 1 cut(s) 674
FspBI CTAG 6 cut(s) 81, 278, 378, 513, 744, 774
GlaI GCGC 3 cut(s) 59, 540, 563
GluI GCNGC 1 cut(s) 674
GsaI CCCAGC 1 cut(s) 790
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 4 cut(s) 256, 586, 742, 1027
HgaI GACGC 1 cut(s) 974
HhaI GCGC 3 cut(s) 60, 541, 564
Hin1II CATG 6 cut(s) 406, 441, 483, 629, 943, 974
Hin6I GCGC 3 cut(s) 58, 539, 562
HinP1I GCGC 3 cut(s) 58, 539, 562
HincII GTYRAC 1 cut(s) 989
HindII GTYRAC 1 cut(s) 989
HindIII AAGCTT 1 cut(s) 831
HinfI GANTC 2 cut(s) 374, 622
HphI GGTGA 3 cut(s) 829, 928, 955
Hpy166II GTNNAC 3 cut(s) 444, 493, 989
Hpy188I TCNGA 2 cut(s) 73, 928
Hpy188III TCNNGA 4 cut(s) 118, 210, 262, 278
Hpy8I GTNNAC 3 cut(s) 444, 493, 989
Hpy99I CGWCG 1 cut(s) 990
HpyCH4III ACNGT 3 cut(s) 23, 284, 667
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 7 cut(s) 127, 334, 416, 431, 444, 479, 890
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 645, 718, 963
HpyF3I CTNAG 3 cut(s) 54, 367, 927
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 6 cut(s) 406, 441, 483, 629, 943, 974
HspAI GCGC 3 cut(s) 58, 539, 562
LmnI GCTCC 4 cut(s) 245, 510, 636, 670
Lsp1109I GCAGC 1 cut(s) 685
LweI GCATC 1 cut(s) 550
MaeI CTAG 6 cut(s) 81, 278, 378, 513, 744, 774
MaeII ACGT 1 cut(s) 321
MbiI CCGCTC 1 cut(s) 639
MboII GAAGA 4 cut(s) 161, 219, 669, 784
MflI RGATCY 6 cut(s) 224, 274, 419, 730, 853, 912
MhlI GDGCHC 6 cut(s) 250, 446, 507, 704, 723, 828
MluCI AATT 6 cut(s) 40, 109, 456, 880, 891, 994
MlyI GAGTC 2 cut(s) 368, 631
MnlI CCTC 7 cut(s) 265, 382, 528, 597, 702, 1017, 1026
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 3 cut(s) 630, 938, 941
MspA1I CMGCKG 1 cut(s) 676
MspR9I CCNGG 1 cut(s) 735
MvaI CCWGG 1 cut(s) 735
MvnI CGCG 2 cut(s) 119, 564
MwoI GCNNNNNNNGC 4 cut(s) 176, 645, 718, 963
NcoI CCATGG 1 cut(s) 939
NdeI CATATG 1 cut(s) 34
NlaIII CATG 6 cut(s) 406, 441, 483, 629, 943, 974
NlaIV GGNNCC 4 cut(s) 506, 701, 720, 875
NruI TCGCGA 1 cut(s) 119
NspV TTCGAA 1 cut(s) 655
OliI CACNNNNGTG 1 cut(s) 941
PaqCI CACCTGC 1 cut(s) 802
PkrI GCNGC 1 cut(s) 675
PleI GAGTC 2 cut(s) 368, 630
PpsI GAGTC 2 cut(s) 368, 630
Ppu21I YACGTR 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 733
PspFI CCCAGC 1 cut(s) 786
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 4 cut(s) 506, 701, 720, 875
PspPI GGNCC 3 cut(s) 740, 873, 1026
PsrI GAACNNNNNNTAC 2 cut(s) 291, 323
PsuI RGATCY 6 cut(s) 224, 274, 419, 730, 853, 912
RruI TCGCGA 1 cut(s) 119
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
RseI CAYNNNNRTG 3 cut(s) 630, 938, 941
SalI GTCGAC 1 cut(s) 987
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 1 cut(s) 674
Sau96I GGNCC 3 cut(s) 740, 873, 1026
SchI GAGTC 2 cut(s) 368, 631
ScrFI CCNGG 1 cut(s) 735
SduI GDGCHC 6 cut(s) 250, 446, 507, 704, 723, 828
SetI ASST 8 cut(s) 13, 324, 383, 599, 697, 816, 835, 994
SfaNI GCATC 1 cut(s) 550
SfuI TTCGAA 1 cut(s) 655
SinI GGWCC 1 cut(s) 873
SmiMI CAYNNNNRTG 3 cut(s) 630, 938, 941
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 6 cut(s) 40, 109, 456, 880, 891, 994
SsiI CCGC 3 cut(s) 564, 639, 676
SspMI CTAG 6 cut(s) 81, 278, 378, 513, 744, 774
StyD4I CCNGG 1 cut(s) 733
StyI CCWWGG 2 cut(s) 743, 939
TaaI ACNGT 3 cut(s) 23, 284, 667
TaiI ACGT 1 cut(s) 324
TaqI TCGA 5 cut(s) 144, 201, 655, 680, 988
TasI AATT 6 cut(s) 40, 109, 456, 880, 891, 994
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 336
TseI GCWGC 1 cut(s) 673
TspDTI ATGAA 2 cut(s) 21, 162
TspGWI ACGGA 1 cut(s) 676
TspRI CASTG 1 cut(s) 336
VneI GTGCAC 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 873
XapI RAATTY 2 cut(s) 40, 880
XbaI TCTAGA 1 cut(s) 277
XcmI CCANNNNNNNNNTGG 2 cut(s) 692, 711
XmaJI CCTAGG 1 cut(s) 743
XmiI GTMKAC 2 cut(s) 492, 988
XspI CTAG 6 cut(s) 81, 278, 378, 513, 744, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.