RLG00000029764

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003472
Physical Location & Seq
Reverse (-)
48738659 .. 48741956
3298 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029764

Sequence Viewer

Length: 654 bp
ATGGCGTTGAGATCGGGCGGCTGGAAGCTCTCATTAGCGATTTTGAGTAGAAAAGCTCCCCCAAAGAAAAAGAAGCCGGACTCTTCCACCGATCCAACTCAGATCCAATCCAATAGAGCCGGAGAGGACGAAACCCGTTGCTGTATTCATGGCTTTCGGTACCAAAGGCGATGTCTATCCAATTGCTCCATTGCGGCAGCCTTTGCTTCCGACCAGAGACAGTACCAAGTTGTTCTTATCACACATTCAGCTCACGAGAGCTTACGTTGTCACTTGGCTGAGAAGAATGTTTCCTATCTTCCAATTTCATCACCACCTGTTCTCTCTATCCATCAGAAACATGATACTACAGGCTCTGTAGAGTTGCCTCTTTCGCTGCAGAAAAAGTTGTTTTCTGGAGAACATAGAAAAGAATGTCTTTCGGTAGTTGAAACGATATTTGGATCAGGCCCAAGCATGGATGATATCAGCTTATTATATTACATGATTCGTGGTCAAGGAACAATCAAATTTGGTCCATGGAATTCCACTGATGGTGGTGATCCATCAGTTGATGATAGCTCTTTAATTCAAATGGCAATAAGATATGGAAAGGATGTTGCAAAACATGATCTGCAGAACTGCAAGAACTGGAATCATTTTCTTAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

24.2

Weight (kDa)

9.06

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBC1 PF14443 172 - 217 2.4e-11 DBC1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 159
AciI CCGC 2 cut(s) 18, 194
AclWI GGATC 4 cut(s) 86, 97, 451, 536
AcsI RAATTY 2 cut(s) 509, 523
AfaI GTAC 2 cut(s) 161, 224
AfiI CCNNNNNNNGG 1 cut(s) 457
AflII CTTAAG 1 cut(s) 644
AgsI TTSAA 2 cut(s) 431, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 6 cut(s) 28, 56, 251, 261, 471, 561
AluI AGCT 6 cut(s) 28, 56, 251, 261, 471, 561
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 4 cut(s) 86, 97, 451, 536
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 2 cut(s) 197, 376
ApoI RAATTY 2 cut(s) 509, 523
ArsI GACNNNNNNTTYG 2 cut(s) 157, 189
Asp718I GGTACC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 449, 515
AsuHPI GGTGA 2 cut(s) 303, 551
AvaII GGWCC 1 cut(s) 515
BanI GGYRCC 1 cut(s) 159
BauI CACGAG 1 cut(s) 254
BbvI GCAGC 2 cut(s) 209, 363
BccI CCATC 3 cut(s) 339, 527, 553
BcoDI GTCTC 1 cut(s) 211
BfmI CTRYAG 4 cut(s) 348, 357, 377, 614
BfrI CTTAAG 1 cut(s) 644
BisI GCNGC 4 cut(s) 19, 195, 198, 377
BlsI GCNGC 4 cut(s) 20, 196, 199, 378
Bme18I GGWCC 1 cut(s) 515
BmgT120I GGNCC 2 cut(s) 449, 515
BmiI GGNNCC 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 518
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 635
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 1 cut(s) 175
BseDI CCNNGG 1 cut(s) 518
BseGI GGATG 2 cut(s) 466, 601
BseJI GATNNNNATC 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 2 cut(s) 113, 270
BseNI ACTGG 1 cut(s) 635
BseXI GCAGC 2 cut(s) 209, 363
BshFI GGCC 1 cut(s) 450
BshNI GGYRCC 1 cut(s) 159
BsiSI CCGG 2 cut(s) 77, 120
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 1 cut(s) 211
BsnI GGCC 1 cut(s) 450
Bsp143I GATC 6 cut(s) 11, 91, 102, 443, 541, 610
Bsp19I CCATGG 1 cut(s) 518
BspACI CCGC 2 cut(s) 18, 194
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 2 cut(s) 112, 271
BspLI GGNNCC 1 cut(s) 161
BspMAI CTGCAG 2 cut(s) 381, 618
BspPI GGATC 4 cut(s) 86, 97, 451, 536
BspT107I GGYRCC 1 cut(s) 159
BspTI CTTAAG 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 189
BsrI ACTGG 1 cut(s) 635
BssECI CCNNGG 1 cut(s) 518
BssMI GATC 6 cut(s) 11, 91, 102, 443, 541, 610
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 1 cut(s) 518
Bst2BI CACGAG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 222
Bst6I CTCTTC 1 cut(s) 88
BstAFI CTTAAG 1 cut(s) 644
BstAPI GCANNNNNTGC 1 cut(s) 203
BstDEI CTNAG 2 cut(s) 99, 279
BstDSI CCRYGG 1 cut(s) 518
BstF5I GGATG 2 cut(s) 466, 601
BstKTI GATC 6 cut(s) 14, 94, 105, 446, 544, 613
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 6 cut(s) 11, 91, 102, 443, 541, 610
BstMWI GCNNNNNNNGC 2 cut(s) 203, 373
BstSFI CTRYAG 4 cut(s) 348, 357, 377, 614
BstV1I GCAGC 2 cut(s) 209, 363
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 518
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 466, 601
BtsIMutI CAGTG 1 cut(s) 528
CaiI CAGNNNCTG 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 449, 515
Csp6I GTAC 2 cut(s) 160, 223
CviAII CATG 6 cut(s) 149, 341, 457, 484, 519, 608
CviQI GTAC 2 cut(s) 160, 223
DdeI CTNAG 2 cut(s) 99, 279
DpnI GATC 6 cut(s) 13, 93, 104, 445, 543, 612
DpnII GATC 6 cut(s) 11, 91, 102, 443, 541, 610
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 518
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 523
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 518
FaeI CATG 6 cut(s) 152, 344, 460, 487, 522, 611
FaiI YATR 9 cut(s) 150, 342, 405, 458, 478, 485, 520, 588, 609
FalI AAGNNNNNCTT 4 cut(s) 219, 251, 402, 434
FatI CATG 6 cut(s) 148, 340, 456, 483, 518, 607
Fnu4HI GCNGC 4 cut(s) 19, 195, 198, 377
FokI GGATG 2 cut(s) 473, 608
Fsp4HI GCNGC 4 cut(s) 19, 195, 198, 377
GluI GCNGC 4 cut(s) 19, 195, 198, 377
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 1 cut(s) 450
HapII CCGG 2 cut(s) 77, 120
Hin1II CATG 6 cut(s) 152, 344, 460, 487, 522, 611
HinfI GANTC 3 cut(s) 80, 487, 634
HpaII CCGG 2 cut(s) 77, 120
HphI GGTGA 2 cut(s) 303, 551
Hpy188I TCNGA 3 cut(s) 102, 211, 336
Hpy188III TCNNGA 2 cut(s) 254, 396
HpyCH4III ACNGT 1 cut(s) 222
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 4 cut(s) 379, 602, 616, 624
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 373
HpyF3I CTNAG 2 cut(s) 99, 279
HpySE526I ACGT 1 cut(s) 265
Hsp92II CATG 6 cut(s) 152, 344, 460, 487, 522, 611
KpnI GGTACC 1 cut(s) 163
Kzo9I GATC 6 cut(s) 11, 91, 102, 443, 541, 610
LmnI GCTCC 2 cut(s) 61, 191
LpnPI CCDG 9 cut(s) 7, 90, 133, 227, 330, 336, 381, 432, 616
Lsp1109I GCAGC 2 cut(s) 209, 363
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 1 cut(s) 269
MalI GATC 6 cut(s) 13, 93, 104, 445, 543, 612
MboI GATC 6 cut(s) 11, 91, 102, 443, 541, 610
MboII GAAGA 3 cut(s) 75, 290, 295
MfeI CAATTG 1 cut(s) 181
MflI RGATCY 1 cut(s) 102
MluCI AATT 5 cut(s) 181, 303, 509, 523, 567
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 119, 234
MnlI CCTC 2 cut(s) 118, 378
MseI TTAA 3 cut(s) 566, 645, 652
MspCI CTTAAG 1 cut(s) 644
MspI CCGG 2 cut(s) 77, 120
MunI CAATTG 1 cut(s) 181
MwoI GCNNNNNNNGC 2 cut(s) 203, 373
NcoI CCATGG 1 cut(s) 518
NdeII GATC 6 cut(s) 11, 91, 102, 443, 541, 610
NlaIII CATG 6 cut(s) 152, 344, 460, 487, 522, 611
NlaIV GGNNCC 1 cut(s) 161
NmuCI GTSAC 1 cut(s) 269
PfeI GAWTC 2 cut(s) 487, 634
PkrI GCNGC 4 cut(s) 20, 196, 199, 378
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 161
PspPI GGNCC 2 cut(s) 449, 515
PstI CTGCAG 2 cut(s) 381, 618
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 2 cut(s) 161, 224
RsaNI GTAC 2 cut(s) 160, 223
SaqAI TTAA 3 cut(s) 566, 645, 652
SatI GCNGC 4 cut(s) 19, 195, 198, 377
Sau3AI GATC 6 cut(s) 11, 91, 102, 443, 541, 610
Sau96I GGNCC 2 cut(s) 449, 515
SchI GAGTC 1 cut(s) 74
SetI ASST 9 cut(s) 30, 58, 253, 263, 268, 319, 473, 563, 651
SfcI CTRYAG 4 cut(s) 348, 357, 377, 614
SinI GGWCC 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 5 cut(s) 181, 303, 509, 523, 567
SsiI CCGC 2 cut(s) 18, 194
StyI CCWWGG 1 cut(s) 518
TaaI ACNGT 1 cut(s) 222
TaiI ACGT 1 cut(s) 268
TasI AATT 5 cut(s) 181, 303, 509, 523, 567
TauI GCSGC 2 cut(s) 21, 197
TfiI GAWTC 2 cut(s) 487, 634
Tru1I TTAA 3 cut(s) 566, 645, 652
Tru9I TTAA 3 cut(s) 566, 645, 652
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 2 cut(s) 197, 376
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 2 cut(s) 137, 297
TspRI CASTG 1 cut(s) 535
Vha464I CTTAAG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 515
XapI RAATTY 2 cut(s) 509, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.