Rh4CG126700

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003472
Physical Location & Seq
Forward (+)
24754452 .. 24766032
11581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG126700.1

Sequence Viewer

Length: 606 bp
ATGTTCCTCTCCGGAAAGATCTCCTCCGCTCCCGAAACCCTAAAATCATTGCCGCTTCTCCTCCTCCTCCACTCGCAACCGTATCCCTGCCGCCCAGTTTTCCCTCCTCGTCTTCTCCGGCGGGACCTCTTTCAACGGCGTCATCGCTCAAGAATTTTAACACTCGCGTCGCCCACGTGCTTCTGGTTTCCAGCGACAGAGGAAGCACCGCCGAGATTATCAGCGTCCTCGGACTGGTTCGTCAGTCTCACTGCTGATGACTACCCTTTTCTCAAACAAGACGATCTTCTTCACATTATGTCATTTCTACCTAAAGATCAGAACTTTGTGAACCATTCATTAGCTGGAAAGAGTAAGAGCTTACGTTGTCACTTGGCTAAGAAGAATGTTTCCTATCTTCCAATTTCATCACCACCTGTTCTCTCTATCCATCAGAAACATGATACTACAGGCTCTGTAGAGTTGCCTCTTTCGCTGCAGAAAAAGTTGTTTTCTGGAGAACATACAAAAGAATGTCTTTCGGCAGTTGAAACGATATTTGGATCAGGCCCAAGCATGGATGGTGATTTCATTGTGATAAATTTCTTTGCTCTGATATCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.53

Weight (kDa)

9.12

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 1 cut(s) 167
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 5 cut(s) 27, 53, 91, 121, 209
AclWI GGATC 1 cut(s) 550
AcsI RAATTY 2 cut(s) 153, 580
AcvI CACGTG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 139
AfiI CCNNNNNNNGG 2 cut(s) 234, 556
AgsI TTSAA 2 cut(s) 134, 530
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 3 cut(s) 344, 360, 602
AluI AGCT 3 cut(s) 344, 360, 602
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 550
AlwNI CAGNNNCTG 1 cut(s) 455
Aor13HI TCCGGA 1 cut(s) 11
AoxI GGCC 1 cut(s) 547
ApeKI GCWGC 1 cut(s) 475
ApoI RAATTY 2 cut(s) 153, 580
AspS9I GGNCC 2 cut(s) 124, 548
AsuHPI GGTGA 2 cut(s) 402, 575
AvaII GGWCC 1 cut(s) 124
BbrPI CACGTG 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 462
BccI CCATC 2 cut(s) 438, 554
BceAI ACGGC 1 cut(s) 152
BciVI GTATCC 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 3 cut(s) 447, 456, 476
BfuI GTATCC 1 cut(s) 93
BglII AGATCT 1 cut(s) 18
BisI GCNGC 3 cut(s) 53, 91, 476
BlsI GCNGC 3 cut(s) 54, 92, 477
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 2 cut(s) 124, 548
BmiI GGNNCC 1 cut(s) 125
BmrI ACTGGG 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 104
BpmI CTGGAG 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 133
BsaAI YACGTR 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 556
Bse1I ACTGG 2 cut(s) 95, 239
Bse3DI GCAATG 1 cut(s) 47
BseAI TCCGGA 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 565
BseLI CCNNNNNNNGG 2 cut(s) 234, 556
BseMI GCAATG 1 cut(s) 47
BseNI ACTGG 2 cut(s) 95, 239
BseRI GAGGAG 5 cut(s) 13, 50, 53, 56, 96
BseXI GCAGC 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 167
BshFI GGCC 1 cut(s) 549
BsiSI CCGG 2 cut(s) 12, 118
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 234, 556
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 1 cut(s) 549
Bsp13I TCCGGA 1 cut(s) 11
Bsp143I GATC 4 cut(s) 18, 283, 316, 542
BspACI CCGC 5 cut(s) 27, 53, 91, 121, 209
BspANI GGCC 1 cut(s) 549
BspEI TCCGGA 1 cut(s) 11
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 1 cut(s) 125
BspMAI CTGCAG 1 cut(s) 480
BspPI GGATC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 47
BsrI ACTGG 2 cut(s) 95, 239
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 4 cut(s) 18, 283, 316, 542
BssNI GRCGYC 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 81
BstACI GRCGYC 1 cut(s) 139
BstBAI YACGTR 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 565
BstFNI CGCG 1 cut(s) 167
BstKTI GATC 4 cut(s) 21, 286, 319, 545
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 4 cut(s) 18, 283, 316, 542
BstMWI GCNNNNNNNGC 1 cut(s) 472
BstSFI CTRYAG 3 cut(s) 447, 456, 476
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 1 cut(s) 462
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BsuI GTATCC 1 cut(s) 93
BsuRI GGCC 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 128
BtsCI GGATG 1 cut(s) 565
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 249
CaiI CAGNNNCTG 1 cut(s) 455
Cfr13I GGNCC 2 cut(s) 124, 548
CseI GACGC 3 cut(s) 128, 156, 213
CviAII CATG 2 cut(s) 440, 556
CviJI RGCY 6 cut(s) 344, 360, 377, 453, 549, 602
CviKI_1 RGCY 6 cut(s) 344, 360, 377, 453, 549, 602
DdeI CTNAG 1 cut(s) 378
DpnI GATC 4 cut(s) 20, 285, 318, 544
DpnII GATC 4 cut(s) 18, 283, 316, 542
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
Eco32I GATATC 1 cut(s) 597
Eco47I GGWCC 1 cut(s) 124
Eco72I CACGTG 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 124
EcoRV GATATC 1 cut(s) 597
FaeI CATG 2 cut(s) 443, 559
FaiI YATR 4 cut(s) 299, 441, 504, 557
FalI AAGNNNNNCTT 4 cut(s) 270, 302, 501, 533
FaqI GGGAC 1 cut(s) 137
FatI CATG 2 cut(s) 439, 555
FauI CCCGC 1 cut(s) 114
Fnu4HI GCNGC 3 cut(s) 53, 91, 476
FokI GGATG 1 cut(s) 572
Fsp4HI GCNGC 3 cut(s) 53, 91, 476
GluI GCNGC 3 cut(s) 53, 91, 476
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 1 cut(s) 549
HapII CCGG 2 cut(s) 12, 118
HgaI GACGC 3 cut(s) 128, 156, 213
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 2 cut(s) 443, 559
HpaII CCGG 2 cut(s) 12, 118
HphI GGTGA 2 cut(s) 402, 575
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 4 cut(s) 232, 321, 435, 594
Hpy188III TCNNGA 4 cut(s) 12, 32, 150, 495
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 1 cut(s) 172
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4IV ACGT 2 cut(s) 176, 364
HpyCH4V TGCA 1 cut(s) 478
HpyF10VI GCNNNNNNNGC 1 cut(s) 472
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 2 cut(s) 176, 364
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 2 cut(s) 443, 559
Kpn2I TCCGGA 1 cut(s) 11
Kzo9I GATC 4 cut(s) 18, 283, 316, 542
LmnI GCTCC 1 cut(s) 34
Lsp1109I GCAGC 1 cut(s) 462
MaeII ACGT 2 cut(s) 176, 364
MaeIII GTNAC 1 cut(s) 368
MalI GATC 4 cut(s) 20, 285, 318, 544
MbiI CCGCTC 1 cut(s) 29
MboI GATC 4 cut(s) 18, 283, 316, 542
MboII GAAGA 5 cut(s) 104, 278, 281, 389, 394
MflI RGATCY 1 cut(s) 18
MluCI AATT 3 cut(s) 153, 402, 580
MroI TCCGGA 1 cut(s) 11
MseI TTAA 2 cut(s) 158, 604
MspI CCGG 2 cut(s) 12, 118
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 472
NdeII GATC 4 cut(s) 18, 283, 316, 542
NlaIII CATG 2 cut(s) 443, 559
NlaIV GGNNCC 1 cut(s) 125
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 54, 92, 477
PmaCI CACGTG 1 cut(s) 177
PmlI CACGTG 1 cut(s) 177
Ppu21I YACGTR 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 124
PspCI CACGTG 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 125
PspPI GGNCC 2 cut(s) 124, 548
PspPPI RGGWCCY 1 cut(s) 124
PstI CTGCAG 1 cut(s) 480
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 1 cut(s) 18
SaqAI TTAA 2 cut(s) 158, 604
SatI GCNGC 3 cut(s) 53, 91, 476
Sau3AI GATC 4 cut(s) 18, 283, 316, 542
Sau96I GGNCC 2 cut(s) 124, 548
SetI ASST 8 cut(s) 129, 179, 313, 346, 362, 367, 418, 604
SfcI CTRYAG 3 cut(s) 447, 456, 476
SinI GGWCC 1 cut(s) 124
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 3 cut(s) 153, 402, 580
SsiI CCGC 5 cut(s) 27, 53, 91, 121, 209
TaaI ACNGT 1 cut(s) 81
TaiI ACGT 2 cut(s) 179, 367
TasI AATT 3 cut(s) 153, 402, 580
TauI GCSGC 2 cut(s) 55, 93
Tru1I TTAA 2 cut(s) 158, 604
Tru9I TTAA 2 cut(s) 158, 604
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 1 cut(s) 475
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 3 cut(s) 327, 396, 559
TspRI CASTG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 2 cut(s) 153, 580
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.