RLG00000030233

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003472
Physical Location & Seq
Reverse (-)
58094985 .. 58096305
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030233

Sequence Viewer

Length: 405 bp
ATGAGGGTTGGGGGACGAAGGTGGGGAGGAGGAAGAAGAGGGTGGGGAGAGTGTGGCGTAGGTGAGGGAAGCGGAGGAGGAGGCGAAGGTGAGGAAGACGAGAAGGGCGAAGAGGTAAAAGGCAAAGAGAGCGGGAGGAGAGCGGAGGAGAGGGAGGCGGTGGTGGCCGTGCTCCACGGCGCGTCGATTATGCTCCGGTTGTCAACCAATTGGGACTGGTTGAAGAGATTGAAACCAGTAATTGTTGAAGAGATTGAAGCTCAGTTTTCAGCCCGGGCTGCTTCCATGGCCATGGTCTTTATCAACAAAGCTGTTCTTCTTATGGAGTACTCGAATTCAATGACACTTCTCACTATGCAGGTAAGATCTTTATTTAGTGGAATATTCAAAATGCATCAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.81

Weight (kDa)

7.89

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccBSI CCGCTC 2 cut(s) 132, 143
AccII CGCG 1 cut(s) 182
AciI CCGC 4 cut(s) 72, 132, 143, 158
AcoI YGGCCR 2 cut(s) 165, 288
AcsI RAATTY 1 cut(s) 334
AfaI GTAC 2 cut(s) 329, 401
AgsI TTSAA 6 cut(s) 223, 232, 248, 257, 339, 388
AluBI AGCT 2 cut(s) 260, 311
AluI AGCT 2 cut(s) 260, 311
Alw21I GWGCWC 1 cut(s) 174
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 2 cut(s) 165, 288
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 1 cut(s) 334
AspLEI GCGC 1 cut(s) 182
AsuC2I CCSGG 2 cut(s) 274, 275
AsuHPI GGTGA 2 cut(s) 74, 101
AvaI CYCGRG 1 cut(s) 273
BalI TGGCCA 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 265
BceAI ACGGC 2 cut(s) 152, 193
BcnI CCSGG 2 cut(s) 274, 275
BfaI CTAG 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 349
BglII AGATCT 1 cut(s) 365
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
BmcAI AGTACT 2 cut(s) 329, 401
Bme1390I CCNGG 2 cut(s) 274, 275
BmeT110I CYCGRG 1 cut(s) 273
BmrFI CCNGG 2 cut(s) 274, 275
BpiI GAAGAC 1 cut(s) 102
BpuMI CCSGG 2 cut(s) 274, 275
BsaJI CCNNGG 4 cut(s) 175, 273, 285, 291
BsaWI WCCGGW 1 cut(s) 195
Bse1I ACTGG 2 cut(s) 221, 236
BseDI CCNNGG 4 cut(s) 175, 273, 285, 291
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 2 cut(s) 221, 236
BseRI GAGGAG 5 cut(s) 42, 90, 93, 151, 161
BseXI GCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 167, 290
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 2 cut(s) 196, 274
BslFI GGGAC 2 cut(s) 27, 227
BsmFI GGGAC 2 cut(s) 27, 227
BsnI GGCC 2 cut(s) 167, 290
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 1 cut(s) 365
Bsp19I CCATGG 2 cut(s) 285, 291
BspACI CCGC 4 cut(s) 72, 132, 143, 158
BspANI GGCC 2 cut(s) 167, 290
BspCNI CTCAG 1 cut(s) 274
BspFNI CGCG 1 cut(s) 182
BspMI ACCTGC 1 cut(s) 349
BsrBI CCGCTC 2 cut(s) 132, 143
BsrI ACTGG 2 cut(s) 221, 236
BssECI CCNNGG 4 cut(s) 175, 273, 285, 291
BssMI GATC 1 cut(s) 365
BssT1I CCWWGG 2 cut(s) 285, 291
Bst6I CTCTTC 4 cut(s) 31, 105, 218, 243
BstDEI CTNAG 1 cut(s) 261
BstDSI CCRYGG 3 cut(s) 175, 285, 291
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 1 cut(s) 368
BstMBI GATC 1 cut(s) 365
BstMWI GCNNNNNNNGC 4 cut(s) 129, 164, 278, 287
BstSCI CCNGG 2 cut(s) 272, 273
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 265
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 365
BstXI CCANNNNNNTGG 1 cut(s) 292
BstYI RGATCY 1 cut(s) 365
BsuRI GGCC 2 cut(s) 167, 290
BtgI CCRYGG 3 cut(s) 175, 285, 291
BveI ACCTGC 1 cut(s) 349
CfoI GCGC 1 cut(s) 182
Cfr9I CCCGGG 1 cut(s) 273
CseI GACGC 1 cut(s) 171
Csp6I GTAC 2 cut(s) 328, 400
CviAII CATG 2 cut(s) 286, 292
CviJI RGCY 6 cut(s) 167, 260, 272, 278, 290, 311
CviKI_1 RGCY 6 cut(s) 167, 260, 272, 278, 290, 311
CviQI GTAC 2 cut(s) 328, 400
DdeI CTNAG 1 cut(s) 261
DpnI GATC 1 cut(s) 367
DpnII GATC 1 cut(s) 365
EaeI YGGCCR 2 cut(s) 165, 288
Eam1104I CTCTTC 4 cut(s) 31, 105, 218, 243
EarI CTCTTC 4 cut(s) 31, 105, 218, 243
Eco130I CCWWGG 2 cut(s) 285, 291
Eco88I CYCGRG 1 cut(s) 273
EcoRI GAATTC 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 285, 291
EcoT22I ATGCAT 1 cut(s) 396
ErhI CCWWGG 2 cut(s) 285, 291
FaeI CATG 2 cut(s) 289, 295
FaiI YATR 5 cut(s) 191, 287, 293, 323, 356
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 2 cut(s) 27, 227
FatI CATG 2 cut(s) 285, 291
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 1 cut(s) 403
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 279
HaeIII GGCC 2 cut(s) 167, 290
HapII CCGG 2 cut(s) 196, 274
HgaI GACGC 1 cut(s) 171
HhaI GCGC 1 cut(s) 182
Hin1II CATG 2 cut(s) 289, 295
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 204
HindII GTYRAC 1 cut(s) 204
HpaII CCGG 2 cut(s) 196, 274
HphI GGTGA 2 cut(s) 74, 101
Hpy166II GTNNAC 1 cut(s) 204
Hpy8I GTNNAC 1 cut(s) 204
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 3 cut(s) 12, 80, 97
HpyCH4V TGCA 2 cut(s) 358, 394
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 164, 278, 287
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 2 cut(s) 289, 295
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 1 cut(s) 365
LmnI GCTCC 2 cut(s) 177, 198
LpnPI CCDG 5 cut(s) 202, 209, 249, 287, 344
Lsp1109I GCAGC 1 cut(s) 265
MaeI CTAG 1 cut(s) 403
MalI GATC 1 cut(s) 367
MbiI CCGCTC 2 cut(s) 132, 143
MboI GATC 1 cut(s) 365
MboII GAAGA 7 cut(s) 45, 48, 107, 122, 235, 260, 308
MfeI CAATTG 1 cut(s) 208
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 174
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 3 cut(s) 208, 240, 334
MluNI TGGCCA 1 cut(s) 290
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 396
MscI TGGCCA 1 cut(s) 290
MslI CAYNNNNRTG 1 cut(s) 290
Msp20I TGGCCA 1 cut(s) 290
MspI CCGG 2 cut(s) 196, 274
MspR9I CCNGG 2 cut(s) 274, 275
MunI CAATTG 1 cut(s) 208
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 129, 164, 278, 287
NciI CCSGG 2 cut(s) 274, 275
NcoI CCATGG 2 cut(s) 285, 291
NdeII GATC 1 cut(s) 365
NlaIII CATG 2 cut(s) 289, 295
NsiI ATGCAT 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PkrI GCNGC 1 cut(s) 280
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 2 cut(s) 329, 401
RsaNI GTAC 2 cut(s) 328, 400
RseI CAYNNNNRTG 1 cut(s) 290
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 1 cut(s) 365
ScaI AGTACT 2 cut(s) 329, 401
ScrFI CCNGG 2 cut(s) 274, 275
SduI GDGCHC 1 cut(s) 174
SetI ASST 7 cut(s) 23, 64, 91, 117, 262, 313, 363
SmaI CCCGGG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 290
SrfI GCCCGGGC 1 cut(s) 275
Sse9I AATT 3 cut(s) 208, 240, 334
SsiI CCGC 4 cut(s) 72, 132, 143, 158
SspI AATATT 1 cut(s) 384
SspMI CTAG 1 cut(s) 403
StyD4I CCNGG 2 cut(s) 272, 273
StyI CCWWGG 2 cut(s) 285, 291
TaqI TCGA 2 cut(s) 185, 332
TasI AATT 3 cut(s) 208, 240, 334
TatI WGTACW 2 cut(s) 327, 399
TseI GCWGC 1 cut(s) 278
TspMI CCCGGG 1 cut(s) 273
XapI RAATTY 1 cut(s) 334
XmaI CCCGGG 1 cut(s) 273
XspI CTAG 1 cut(s) 403
ZrmI AGTACT 2 cut(s) 329, 401
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.