RLG00000030659

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002081
Physical Location & Seq
Reverse (-)
65226511 .. 65229259
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030659

Sequence Viewer

Length: 528 bp
ATGGGCGATTGCTGTTGTCGAGCCTGGGACCGCGTCTCGTCCTCGATGGGGATAAGGAGGAGCCAAAGGAGCTTGCTGCATGATGAAAAGGCCGGCTGGAGGGCATGGGACGTTTTAGATGGAGATGAGAAGGGCAAGGTAAAGAAGGTGTTGATGGAGATGAGAAGGGCATGGGAAGTTTTAGATGGAGATGAGAAGGGCAAGGGTGTTGACGGAAATGAGAAGGGCAAGGGAAAGGAGGTGTCAAAGAGCTTGCCGAGTTTGTCTGAGATTCTGATCAAGTACATAACATTGAAGGAGCAAAAAGTGTTGGACCATGAGAAGGTTCAGGTGGAGCAAGAGAAGACCAGGGTTCAGAAGTTGTTTAATGGGTTGCAGAACTCGATAAACATTTACAATGCCAGTGGAAACCCCCAAATCTCCAAAGTTTCGGATGCGGCACCGAAACAGATGCACATGGCTCCTCAGTCTCGCCTACCTAATGGGTCTTCTACAGGTACAATCCTAACCCCATCTGCTTGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.49

Weight (kDa)

9.16

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 439
AccII CGCG 1 cut(s) 33
AciI CCGC 2 cut(s) 31, 437
AfaI GTAC 2 cut(s) 284, 499
AfiI CCNNNNNNNGG 3 cut(s) 48, 99, 322
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 2 cut(s) 23, 347
AluBI AGCT 2 cut(s) 72, 252
AluI AGCT 2 cut(s) 72, 252
Alw26I GTCTC 2 cut(s) 40, 474
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 76
AspS9I GGNCC 2 cut(s) 28, 313
AvaII GGWCC 2 cut(s) 28, 313
BanI GGYRCC 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 350, 480
BbvI GCAGC 1 cut(s) 63
BccI CCATC 5 cut(s) 40, 113, 148, 179, 520
BcgI CGANNNNNNTGC 2 cut(s) 433, 467
BciT130I CCWGG 2 cut(s) 25, 349
BclI TGATCA 1 cut(s) 276
BcoDI GTCTC 2 cut(s) 40, 474
BfmI CTRYAG 1 cut(s) 492
BisI GCNGC 2 cut(s) 77, 438
BlsI GCNGC 2 cut(s) 78, 439
Bme1390I CCNGG 2 cut(s) 25, 349
Bme18I GGWCC 2 cut(s) 28, 313
BmgT120I GGNCC 2 cut(s) 28, 313
BmiI GGNNCC 4 cut(s) 29, 62, 441, 462
BmrFI CCNGG 2 cut(s) 25, 349
BmsI GCATC 2 cut(s) 424, 441
BpiI GAAGAC 2 cut(s) 350, 480
BpmI CTGGAG 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 24, 348
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 99, 322
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 275
BseBI CCWGG 2 cut(s) 25, 349
BseDI CCNNGG 2 cut(s) 24, 348
BseGI GGATG 1 cut(s) 439
BseJI GATNNNNATC 1 cut(s) 275
BseLI CCNNNNNNNGG 3 cut(s) 48, 99, 322
BseMII CTCAG 2 cut(s) 258, 479
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 2 cut(s) 73, 453
BseXI GCAGC 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 439
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 41, 122
BslI CCNNNNNNNGG 3 cut(s) 48, 99, 322
BsmAI GTCTC 2 cut(s) 40, 474
BsmBI CGTCTC 1 cut(s) 40
BsmFI GGGAC 2 cut(s) 41, 122
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 276
BspACI CCGC 2 cut(s) 31, 437
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 2 cut(s) 259, 478
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 4 cut(s) 29, 62, 441, 462
BspT107I GGYRCC 1 cut(s) 439
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 24, 348
BssMI GATC 1 cut(s) 276
Bst2UI CCWGG 2 cut(s) 25, 349
BstC8I GCNNGC 3 cut(s) 74, 94, 254
BstDEI CTNAG 2 cut(s) 267, 465
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 1 cut(s) 33
BstKTI GATC 1 cut(s) 279
BstMAI GTCTC 2 cut(s) 40, 474
BstMBI GATC 1 cut(s) 276
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 2 cut(s) 25, 349
BstSCI CCNGG 2 cut(s) 23, 347
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 63
BstV2I GAAGAC 2 cut(s) 350, 480
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 439
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 3 cut(s) 74, 94, 254
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 28, 313
CseI GACGC 1 cut(s) 22
Csp6I GTAC 2 cut(s) 283, 498
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 5 cut(s) 80, 105, 171, 317, 457
CviJI RGCY 7 cut(s) 23, 63, 72, 92, 96, 252, 461
CviKI_1 RGCY 7 cut(s) 23, 63, 72, 92, 96, 252, 461
CviQI GTAC 2 cut(s) 283, 498
DdeI CTNAG 2 cut(s) 267, 465
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
Eco47I GGWCC 2 cut(s) 28, 313
EcoRII CCWGG 2 cut(s) 23, 347
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 5 cut(s) 83, 108, 174, 320, 460
FaiI YATR 7 cut(s) 81, 106, 172, 287, 318, 458, 524
FaqI GGGAC 2 cut(s) 41, 122
FatI CATG 5 cut(s) 79, 104, 170, 316, 456
FbaI TGATCA 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 77, 438
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 2 cut(s) 77, 438
GluI GCNGC 2 cut(s) 77, 438
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 22
Hin1II CATG 5 cut(s) 83, 108, 174, 320, 460
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 1 cut(s) 271
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 4 cut(s) 268, 276, 357, 433
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 7 cut(s) 124, 139, 159, 190, 217, 289, 316
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 3 cut(s) 79, 376, 454
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 2 cut(s) 267, 465
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 5 cut(s) 83, 108, 174, 320, 460
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 276
Kzo9I GATC 1 cut(s) 276
LmnI GCTCC 5 cut(s) 60, 69, 298, 334, 466
LpnPI CCDG 9 cut(s) 10, 37, 82, 106, 314, 334, 361, 415, 480
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 2 cut(s) 424, 441
MaeII ACGT 1 cut(s) 111
MalI GATC 1 cut(s) 278
MboI GATC 1 cut(s) 276
MboII GAAGA 2 cut(s) 355, 480
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 5 cut(s) 51, 52, 93, 232, 474
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 1 cut(s) 366
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 2 cut(s) 25, 349
MvaI CCWGG 2 cut(s) 25, 349
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 69
NaeI GCCGGC 1 cut(s) 94
NdeII GATC 1 cut(s) 276
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 5 cut(s) 83, 108, 174, 320, 460
NlaIV GGNNCC 4 cut(s) 29, 62, 441, 462
NmeAIII GCCGAG 1 cut(s) 282
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 271
PflFI GACNNNGTC 1 cut(s) 32
PkrI GCNGC 2 cut(s) 78, 439
Psp6I CCWGG 2 cut(s) 23, 347
PspGI CCWGG 2 cut(s) 23, 347
PspN4I GGNNCC 4 cut(s) 29, 62, 441, 462
PspPI GGNCC 2 cut(s) 28, 313
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 2 cut(s) 284, 499
RsaNI GTAC 2 cut(s) 283, 498
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 2 cut(s) 77, 438
Sau3AI GATC 1 cut(s) 276
Sau96I GGNCC 2 cut(s) 28, 313
ScrFI CCNGG 2 cut(s) 25, 349
SfaNI GCATC 2 cut(s) 424, 441
SfcI CTRYAG 1 cut(s) 492
SinI GGWCC 2 cut(s) 28, 313
SsiI CCGC 2 cut(s) 31, 437
StyD4I CCNGG 2 cut(s) 23, 347
TaiI ACGT 1 cut(s) 114
TaqI TCGA 3 cut(s) 19, 44, 383
TatI WGTACW 1 cut(s) 282
TauI GCSGC 1 cut(s) 440
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 1 cut(s) 409
TseI GCWGC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 2 cut(s) 28, 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.