Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 2455, 2668 |
| AccB1I | GGYRCC | 1 cut(s) | 1453 |
| AccII | CGCG | 5 cut(s) | 66, 72, 374, 420, 1585 |
| AccIII | TCCGGA | 2 cut(s) | 557, 2695 |
| AciI | CCGC | 6 cut(s) | 61, 64, 381, 418, 1583, 2855 |
| AcoI | YGGCCR | 6 cut(s) | 24, 288, 400, 415, 1543, 3234 |
| AcsI | RAATTY | 7 cut(s) | 332, 1256, 1316, 1702, 2089, 2167, 2684 |
| AcuI | CTGAAG | 1 cut(s) | 1918 |
| AfaI | GTAC | 4 cut(s) | 1105, 1359, 2618, 2653 |
| AflIII | ACRYGT | 1 cut(s) | 1695 |
| AgsI | TTSAA | 7 cut(s) | 100, 262, 1702, 2080, 2150, 2159, 2525 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1039 |
| AjiI | CACGTC | 1 cut(s) | 133 |
| AjnI | CCWGG | 7 cut(s) | 977, 1495, 1519, 1587, 1615, 1988, 3255 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2190, 2222 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1491, 1523 |
| Alw21I | GWGCWC | 4 cut(s) | 51, 1312, 1680, 2190 |
| Alw26I | GTCTC | 9 cut(s) | 120, 272, 436, 1047, 1195, 1606, 1672, 2220, 2736 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 596, 2114 |
| Aor13HI | TCCGGA | 2 cut(s) | 557, 2695 |
| ApaI | GGGCCC | 1 cut(s) | 2512 |
| ApeKI | GCWGC | 3 cut(s) | 367, 644, 1217 |
| ApoI | RAATTY | 7 cut(s) | 332, 1256, 1316, 1702, 2089, 2167, 2684 |
| AseI | ATTAAT | 2 cut(s) | 782, 1425 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 332, 2298 |
| AspLEI | GCGC | 5 cut(s) | 72, 219, 376, 422, 1587 |
| AspS9I | GGNCC | 9 cut(s) | 767, 1063, 1273, 1660, 1904, 2508, 2509, 3065, 3248 |
| AsuHPI | GGTGA | 4 cut(s) | 305, 1147, 2836, 3112 |
| AsuII | TTCGAA | 1 cut(s) | 1420 |
| AsuNHI | GCTAGC | 1 cut(s) | 2714 |
| AvaII | GGWCC | 3 cut(s) | 1273, 1660, 1904 |
| BaeGI | GKGCMC | 1 cut(s) | 2512 |
| BalI | TGGCCA | 3 cut(s) | 402, 1545, 3236 |
| BamHI | GGATCC | 6 cut(s) | 570, 625, 903, 1111, 1768, 1896 |
| BanI | GGYRCC | 1 cut(s) | 1453 |
| BanII | GRGCYC | 3 cut(s) | 51, 141, 2512 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1385, 1417 |
| BauI | CACGAG | 1 cut(s) | 1305 |
| BbsI | GAAGAC | 2 cut(s) | 312, 1924 |
| Bbv12I | GWGCWC | 4 cut(s) | 51, 1312, 1680, 2190 |
| BbvI | GCAGC | 3 cut(s) | 379, 631, 1204 |
| BceAI | ACGGC | 3 cut(s) | 430, 667, 1993 |
| BciT130I | CCWGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| BclI | TGATCA | 4 cut(s) | 855, 988, 1513, 1714 |
| BcoDI | GTCTC | 9 cut(s) | 120, 272, 436, 1047, 1195, 1606, 1672, 2220, 2736 |
| BfaI | CTAG | 4 cut(s) | 1602, 2243, 2271, 2715 |
| BfmI | CTRYAG | 2 cut(s) | 713, 2610 |
| BfoI | RGCGCY | 1 cut(s) | 220 |
| BfuAI | ACCTGC | 2 cut(s) | 2455, 2668 |
| BisI | GCNGC | 5 cut(s) | 64, 368, 418, 645, 1218 |
| BlsI | GCNGC | 5 cut(s) | 65, 369, 419, 646, 1219 |
| Bme1390I | CCNGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| Bme18I | GGWCC | 3 cut(s) | 1273, 1660, 1904 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1039 |
| BmgBI | CACGTC | 1 cut(s) | 133 |
| BmgT120I | GGNCC | 9 cut(s) | 767, 1063, 1273, 1660, 1904, 2508, 2509, 3065, 3248 |
| BmrFI | CCNGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| BmrI | ACTGGG | 2 cut(s) | 182, 1172 |
| BmsI | GCATC | 8 cut(s) | 376, 913, 1951, 2169, 2240, 2288, 2391, 2920 |
| BmtI | GCTAGC | 1 cut(s) | 2718 |
| BmuI | ACTGGG | 2 cut(s) | 182, 1172 |
| BpiI | GAAGAC | 2 cut(s) | 312, 1924 |
| BpmI | CTGGAG | 4 cut(s) | 1662, 2147, 2606, 3278 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2184 |
| Bpu14I | TTCGAA | 1 cut(s) | 1420 |
| BpuEI | CTTGAG | 1 cut(s) | 3110 |
| Bsa29I | ATCGAT | 2 cut(s) | 1840, 2630 |
| BsaBI | GATNNNNATC | 2 cut(s) | 927, 1713 |
| BsaI | GGTCTC | 3 cut(s) | 436, 1195, 1606 |
| BsaWI | WCCGGW | 4 cut(s) | 39, 557, 1084, 2695 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 117, 147, 2579, 2609 |
| Bse118I | RCCGGY | 2 cut(s) | 26, 290 |
| Bse1I | ACTGG | 5 cut(s) | 188, 605, 1178, 1801, 1927 |
| Bse3DI | GCAATG | 5 cut(s) | 396, 1395, 2404, 2542, 2969 |
| Bse8I | GATNNNNATC | 2 cut(s) | 927, 1713 |
| BseAI | TCCGGA | 2 cut(s) | 557, 2695 |
| BseBI | CCWGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| BseCI | ATCGAT | 2 cut(s) | 1840, 2630 |
| BseJI | GATNNNNATC | 2 cut(s) | 927, 1713 |
| BseMI | GCAATG | 5 cut(s) | 396, 1395, 2404, 2542, 2969 |
| BseMII | CTCAG | 7 cut(s) | 257, 603, 960, 1037, 1823, 2175, 2382 |
| BseNI | ACTGG | 5 cut(s) | 188, 605, 1178, 1801, 1927 |
| BseRI | GAGGAG | 5 cut(s) | 415, 424, 1119, 2847, 2937 |
| BseSI | GKGCMC | 1 cut(s) | 2512 |
| BseX3I | CGGCCG | 1 cut(s) | 415 |
| BseXI | GCAGC | 3 cut(s) | 379, 631, 1204 |
| Bsh1236I | CGCG | 5 cut(s) | 66, 72, 374, 420, 1585 |
| Bsh1285I | CGRYCG | 1 cut(s) | 418 |
| BshNI | GGYRCC | 1 cut(s) | 1453 |
| BshVI | ATCGAT | 2 cut(s) | 1840, 2630 |
| BsiEI | CGRYCG | 1 cut(s) | 418 |
| BsiHKAI | GWGCWC | 4 cut(s) | 51, 1312, 1680, 2190 |
| BsiSI | CCGG | 8 cut(s) | 27, 40, 196, 291, 558, 1085, 2696, 3024 |
| BslFI | GGGAC | 1 cut(s) | 1259 |
| BsmAI | GTCTC | 9 cut(s) | 120, 272, 436, 1047, 1195, 1606, 1672, 2220, 2736 |
| BsmBI | CGTCTC | 2 cut(s) | 120, 272 |
| BsmFI | GGGAC | 1 cut(s) | 1259 |
| BsmI | GAATGC | 1 cut(s) | 1657 |
| Bso31I | GGTCTC | 3 cut(s) | 436, 1195, 1606 |
| Bsp119I | TTCGAA | 1 cut(s) | 1420 |
| Bsp120I | GGGCCC | 1 cut(s) | 2508 |
| Bsp1286I | GDGCHC | 6 cut(s) | 51, 141, 1312, 1680, 2190, 2512 |
| Bsp13I | TCCGGA | 2 cut(s) | 557, 2695 |
| Bsp19I | CCATGG | 4 cut(s) | 397, 621, 2410, 2621 |
| BspACI | CCGC | 6 cut(s) | 61, 64, 381, 418, 1583, 2855 |
| BspCNI | CTCAG | 7 cut(s) | 256, 602, 959, 1038, 1822, 2176, 2381 |
| BspDI | ATCGAT | 2 cut(s) | 1840, 2630 |
| BspEI | TCCGGA | 2 cut(s) | 557, 2695 |
| BspFNI | CGCG | 5 cut(s) | 66, 72, 374, 420, 1585 |
| BspHI | TCATGA | 1 cut(s) | 1761 |
| BspMI | ACCTGC | 2 cut(s) | 2455, 2668 |
| BspOI | GCTAGC | 1 cut(s) | 2718 |
| BspQI | GCTCTTC | 1 cut(s) | 3089 |
| BspT104I | TTCGAA | 1 cut(s) | 1420 |
| BspT107I | GGYRCC | 1 cut(s) | 1453 |
| BspTNI | GGTCTC | 3 cut(s) | 436, 1195, 1606 |
| BsrDI | GCAATG | 5 cut(s) | 396, 1395, 2404, 2542, 2969 |
| BsrFI | RCCGGY | 2 cut(s) | 26, 290 |
| BsrI | ACTGG | 5 cut(s) | 188, 605, 1178, 1801, 1927 |
| BssAI | RCCGGY | 2 cut(s) | 26, 290 |
| BssSI | CACGAG | 1 cut(s) | 1305 |
| BssT1I | CCWWGG | 7 cut(s) | 397, 621, 1656, 1900, 2410, 2512, 2621 |
| Bst2BI | CACGAG | 1 cut(s) | 1305 |
| Bst2UI | CCWGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| Bst4CI | ACNGT | 7 cut(s) | 936, 1108, 1150, 1861, 2432, 2614, 2908 |
| Bst6I | CTCTTC | 5 cut(s) | 1388, 1401, 2078, 2133, 3089 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2544 |
| BstBI | TTCGAA | 1 cut(s) | 1420 |
| BstDEI | CTNAG | 9 cut(s) | 243, 589, 946, 1046, 1263, 1346, 1809, 2184, 2368 |
| BstDSI | CCRYGG | 5 cut(s) | 397, 621, 1490, 2410, 2621 |
| BstFNI | CGCG | 5 cut(s) | 66, 72, 374, 420, 1585 |
| BstH2I | RGCGCY | 1 cut(s) | 220 |
| BstHHI | GCGC | 5 cut(s) | 72, 219, 376, 422, 1587 |
| BstMAI | GTCTC | 9 cut(s) | 120, 272, 436, 1047, 1195, 1606, 1672, 2220, 2736 |
| BstMCI | CGRYCG | 1 cut(s) | 418 |
| BstNI | CCWGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| BstNSI | RCATGY | 3 cut(s) | 527, 1699, 1998 |
| BstSCI | CCNGG | 7 cut(s) | 977, 1495, 1519, 1587, 1615, 1988, 3255 |
| BstSFI | CTRYAG | 2 cut(s) | 713, 2610 |
| BstSLI | GKGCMC | 1 cut(s) | 2512 |
| BstUI | CGCG | 5 cut(s) | 66, 72, 374, 420, 1585 |
| BstV1I | GCAGC | 3 cut(s) | 379, 631, 1204 |
| BstV2I | GAAGAC | 2 cut(s) | 312, 1924 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 122 |
| BstZI | CGGCCG | 1 cut(s) | 415 |
| Bsu15I | ATCGAT | 2 cut(s) | 1840, 2630 |
| BsuTUI | ATCGAT | 2 cut(s) | 1840, 2630 |
| BtgI | CCRYGG | 5 cut(s) | 397, 621, 1490, 2410, 2621 |
| BtrI | CACGTC | 1 cut(s) | 133 |
| BtsI | GCAGTG | 1 cut(s) | 1173 |
| BtsIMutI | CAGTG | 8 cut(s) | 251, 612, 932, 1113, 1146, 1173, 2258, 2619 |
| BveI | ACCTGC | 2 cut(s) | 2455, 2668 |
| CaiI | CAGNNNCTG | 2 cut(s) | 596, 2114 |
| CciI | TCATGA | 1 cut(s) | 1761 |
| CfoI | GCGC | 5 cut(s) | 72, 219, 376, 422, 1587 |
| Cfr10I | RCCGGY | 2 cut(s) | 26, 290 |
| Cfr13I | GGNCC | 9 cut(s) | 767, 1063, 1273, 1660, 1904, 2508, 2509, 3065, 3248 |
| ClaI | ATCGAT | 2 cut(s) | 1840, 2630 |
| Csp6I | GTAC | 4 cut(s) | 1104, 1358, 2617, 2652 |
| CviQI | GTAC | 4 cut(s) | 1104, 1358, 2617, 2652 |
| DdeI | CTNAG | 9 cut(s) | 243, 589, 946, 1046, 1263, 1346, 1809, 2184, 2368 |
| DraI | TTTAAA | 3 cut(s) | 1414, 2455, 2959 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1039 |
| EaeI | YGGCCR | 6 cut(s) | 24, 288, 400, 415, 1543, 3234 |
| EagI | CGGCCG | 1 cut(s) | 415 |
| Eam1104I | CTCTTC | 5 cut(s) | 1388, 1401, 2078, 2133, 3089 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1039 |
| EarI | CTCTTC | 5 cut(s) | 1388, 1401, 2078, 2133, 3089 |
| EciI | GGCGGA | 2 cut(s) | 50, 370 |
| Ecl136II | GAGCTC | 1 cut(s) | 49 |
| EclXI | CGGCCG | 1 cut(s) | 415 |
| Eco130I | CCWWGG | 7 cut(s) | 397, 621, 1656, 1900, 2410, 2512, 2621 |
| Eco24I | GRGCYC | 3 cut(s) | 51, 141, 2512 |
| Eco31I | GGTCTC | 3 cut(s) | 436, 1195, 1606 |
| Eco32I | GATATC | 1 cut(s) | 508 |
| Eco47I | GGWCC | 3 cut(s) | 1273, 1660, 1904 |
| Eco52I | CGGCCG | 1 cut(s) | 415 |
| Eco53kI | GAGCTC | 1 cut(s) | 49 |
| Eco57I | CTGAAG | 1 cut(s) | 1918 |
| EcoICRI | GAGCTC | 1 cut(s) | 49 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1273, 1904, 2509, 3065 |
| EcoRI | GAATTC | 1 cut(s) | 332 |
| EcoRII | CCWGG | 7 cut(s) | 977, 1495, 1519, 1587, 1615, 1988, 3255 |
| EcoRV | GATATC | 1 cut(s) | 508 |
| EcoT14I | CCWWGG | 7 cut(s) | 397, 621, 1656, 1900, 2410, 2512, 2621 |
| EcoT22I | ATGCAT | 2 cut(s) | 928, 2406 |
| EcoT38I | GRGCYC | 3 cut(s) | 51, 141, 2512 |
| ErhI | CCWWGG | 7 cut(s) | 397, 621, 1656, 1900, 2410, 2512, 2621 |
| Esp3I | CGTCTC | 2 cut(s) | 120, 272 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 3071, 3103 |
| FaqI | GGGAC | 1 cut(s) | 1259 |
| FbaI | TGATCA | 4 cut(s) | 855, 988, 1513, 1714 |
| Fnu4HI | GCNGC | 5 cut(s) | 64, 368, 418, 645, 1218 |
| FriOI | GRGCYC | 3 cut(s) | 51, 141, 2512 |
| Fsp4HI | GCNGC | 5 cut(s) | 64, 368, 418, 645, 1218 |
| FspBI | CTAG | 4 cut(s) | 1602, 2243, 2271, 2715 |
| GlaI | GCGC | 5 cut(s) | 71, 218, 375, 421, 1586 |
| GluI | GCNGC | 5 cut(s) | 64, 368, 418, 645, 1218 |
| GsuI | CTGGAG | 4 cut(s) | 1662, 2147, 2606, 3278 |
| HaeII | RGCGCY | 1 cut(s) | 220 |
| HapII | CCGG | 8 cut(s) | 27, 40, 196, 291, 558, 1085, 2696, 3024 |
| HhaI | GCGC | 5 cut(s) | 72, 219, 376, 422, 1587 |
| Hin6I | GCGC | 5 cut(s) | 70, 217, 374, 420, 1585 |
| HinP1I | GCGC | 5 cut(s) | 70, 217, 374, 420, 1585 |
| HincII | GTYRAC | 1 cut(s) | 1183 |
| HindII | GTYRAC | 1 cut(s) | 1183 |
| HpaII | CCGG | 8 cut(s) | 27, 40, 196, 291, 558, 1085, 2696, 3024 |
| HphI | GGTGA | 4 cut(s) | 305, 1147, 2836, 3112 |
| Hpy166II | GTNNAC | 6 cut(s) | 1171, 1183, 1470, 1623, 2258, 2617 |
| Hpy8I | GTNNAC | 6 cut(s) | 1171, 1183, 1470, 1623, 2258, 2617 |
| Hpy99I | CGWCG | 5 cut(s) | 56, 77, 134, 152, 167 |
| HpyCH4III | ACNGT | 7 cut(s) | 936, 1108, 1150, 1861, 2432, 2614, 2908 |
| HpyCH4IV | ACGT | 2 cut(s) | 132, 265 |
| HpyF3I | CTNAG | 9 cut(s) | 243, 589, 946, 1046, 1263, 1346, 1809, 2184, 2368 |
| HpySE526I | ACGT | 2 cut(s) | 132, 265 |
| HspAI | GCGC | 5 cut(s) | 70, 217, 374, 420, 1585 |
| KflI | GGGWCCC | 1 cut(s) | 1273 |
| Kpn2I | TCCGGA | 2 cut(s) | 557, 2695 |
| KroI | GCCGGC | 1 cut(s) | 26 |
| KroNI | GCCGGC | 1 cut(s) | 28 |
| Ksp22I | TGATCA | 4 cut(s) | 855, 988, 1513, 1714 |
| LguI | GCTCTTC | 1 cut(s) | 3089 |
| LmnI | GCTCC | 6 cut(s) | 91, 399, 548, 1643, 1969, 2587 |
| Lsp1109I | GCAGC | 3 cut(s) | 379, 631, 1204 |
| LweI | GCATC | 8 cut(s) | 376, 913, 1951, 2169, 2240, 2288, 2391, 2920 |
| MaeI | CTAG | 4 cut(s) | 1602, 2243, 2271, 2715 |
| MaeII | ACGT | 2 cut(s) | 132, 265 |
| MaeIII | GTNAC | 4 cut(s) | 170, 1135, 2371, 2908 |
| MfeI | CAATTG | 1 cut(s) | 2520 |
| MhlI | GDGCHC | 6 cut(s) | 51, 141, 1312, 1680, 2190, 2512 |
| MlsI | TGGCCA | 3 cut(s) | 402, 1545, 3236 |
| MluNI | TGGCCA | 3 cut(s) | 402, 1545, 3236 |
| MlyI | GAGTC | 4 cut(s) | 1674, 1757, 3065, 3148 |
| MmeI | TCCRAC | 6 cut(s) | 33, 671, 1897, 2175, 2992, 3222 |
| Mox20I | TGGCCA | 3 cut(s) | 402, 1545, 3236 |
| Mph1103I | ATGCAT | 2 cut(s) | 928, 2406 |
| MroI | TCCGGA | 2 cut(s) | 557, 2695 |
| MroNI | GCCGGC | 1 cut(s) | 26 |
| MroXI | GAANNNNTTC | 2 cut(s) | 332, 2298 |
| MscI | TGGCCA | 3 cut(s) | 402, 1545, 3236 |
| MslI | CAYNNNNRTG | 5 cut(s) | 732, 1877, 2409, 2959, 3170 |
| Msp20I | TGGCCA | 3 cut(s) | 402, 1545, 3236 |
| MspI | CCGG | 8 cut(s) | 27, 40, 196, 291, 558, 1085, 2696, 3024 |
| MspR9I | CCNGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| MunI | CAATTG | 1 cut(s) | 2520 |
| Mva1269I | GAATGC | 1 cut(s) | 1657 |
| MvaI | CCWGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| MvnI | CGCG | 5 cut(s) | 66, 72, 374, 420, 1585 |
| NaeI | GCCGGC | 1 cut(s) | 28 |
| NcoI | CCATGG | 4 cut(s) | 397, 621, 2410, 2621 |
| NgoMIV | GCCGGC | 1 cut(s) | 26 |
| NheI | GCTAGC | 1 cut(s) | 2714 |
| NmeAIII | GCCGAG | 1 cut(s) | 245 |
| NmuCI | GTSAC | 2 cut(s) | 1135, 2908 |
| NsiI | ATGCAT | 2 cut(s) | 928, 2406 |
| NspI | RCATGY | 3 cut(s) | 527, 1699, 1998 |
| NspV | TTCGAA | 1 cut(s) | 1420 |
| PagI | TCATGA | 1 cut(s) | 1761 |
| PciI | ACATGT | 1 cut(s) | 1695 |
| PciSI | GCTCTTC | 1 cut(s) | 3089 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 171 |
| PctI | GAATGC | 1 cut(s) | 1657 |
| PdiI | GCCGGC | 1 cut(s) | 28 |
| PdmI | GAANNNNTTC | 2 cut(s) | 332, 2298 |
| PfeI | GAWTC | 8 cut(s) | 80, 258, 910, 982, 1528, 2133, 2632, 3027 |
| PflFI | GACNNNGTC | 1 cut(s) | 1187 |
| PfoI | TCCNGGA | 2 cut(s) | 977, 3255 |
| PkrI | GCNGC | 5 cut(s) | 65, 369, 419, 646, 1219 |
| PleI | GAGTC | 4 cut(s) | 1673, 1756, 3064, 3148 |
| PpsI | GAGTC | 4 cut(s) | 1673, 1756, 3064, 3148 |
| PpuMI | RGGWCCY | 2 cut(s) | 1273, 1904 |
| PscI | ACATGT | 1 cut(s) | 1695 |
| PshBI | ATTAAT | 2 cut(s) | 782, 1425 |
| Psp124BI | GAGCTC | 1 cut(s) | 51 |
| Psp5II | RGGWCCY | 2 cut(s) | 1273, 1904 |
| Psp6I | CCWGG | 7 cut(s) | 977, 1495, 1519, 1587, 1615, 1988, 3255 |
| PspGI | CCWGG | 7 cut(s) | 977, 1495, 1519, 1587, 1615, 1988, 3255 |
| PspOMI | GGGCCC | 1 cut(s) | 2508 |
| PspPI | GGNCC | 9 cut(s) | 767, 1063, 1273, 1660, 1904, 2508, 2509, 3065, 3248 |
| PspPPI | RGGWCCY | 2 cut(s) | 1273, 1904 |
| PstNI | CAGNNNCTG | 2 cut(s) | 596, 2114 |
| PsyI | GACNNNGTC | 1 cut(s) | 1187 |
| RsaI | GTAC | 4 cut(s) | 1105, 1359, 2618, 2653 |
| RsaNI | GTAC | 4 cut(s) | 1104, 1358, 2617, 2652 |
| RseI | CAYNNNNRTG | 5 cut(s) | 732, 1877, 2409, 2959, 3170 |
| SacI | GAGCTC | 1 cut(s) | 51 |
| SapI | GCTCTTC | 1 cut(s) | 3089 |
| SatI | GCNGC | 5 cut(s) | 64, 368, 418, 645, 1218 |
| Sau96I | GGNCC | 9 cut(s) | 767, 1063, 1273, 1660, 1904, 2508, 2509, 3065, 3248 |
| SchI | GAGTC | 4 cut(s) | 1674, 1757, 3065, 3148 |
| ScrFI | CCNGG | 7 cut(s) | 979, 1497, 1521, 1589, 1617, 1990, 3257 |
| SduI | GDGCHC | 6 cut(s) | 51, 141, 1312, 1680, 2190, 2512 |
| SfaNI | GCATC | 8 cut(s) | 376, 913, 1951, 2169, 2240, 2288, 2391, 2920 |
| SfcI | CTRYAG | 2 cut(s) | 713, 2610 |
| SfuI | TTCGAA | 1 cut(s) | 1420 |
| SinI | GGWCC | 3 cut(s) | 1273, 1660, 1904 |
| SmiI | ATTTAAAT | 1 cut(s) | 2959 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 732, 1877, 2409, 2959, 3170 |
| SmlI | CTYRAG | 1 cut(s) | 3125 |
| SmoI | CTYRAG | 1 cut(s) | 3125 |
| SsiI | CCGC | 6 cut(s) | 61, 64, 381, 418, 1583, 2855 |
| SspI | AATATT | 3 cut(s) | 2529, 2710, 3183 |
| SspMI | CTAG | 4 cut(s) | 1602, 2243, 2271, 2715 |
| SstI | GAGCTC | 1 cut(s) | 51 |
| StyD4I | CCNGG | 7 cut(s) | 977, 1495, 1519, 1587, 1615, 1988, 3255 |
| StyI | CCWWGG | 7 cut(s) | 397, 621, 1656, 1900, 2410, 2512, 2621 |
| SwaI | ATTTAAAT | 1 cut(s) | 2959 |
| TaaI | ACNGT | 7 cut(s) | 936, 1108, 1150, 1861, 2432, 2614, 2908 |
| TaiI | ACGT | 2 cut(s) | 135, 268 |
| TaqI | TCGA | 9 cut(s) | 342, 830, 868, 1032, 1420, 1840, 2136, 2503, 2630 |
| TaqII | GACCGA | 1 cut(s) | 418 |
| TatI | WGTACW | 2 cut(s) | 1103, 2651 |
| TauI | GCSGC | 2 cut(s) | 66, 420 |
| TfiI | GAWTC | 8 cut(s) | 80, 258, 910, 982, 1528, 2133, 2632, 3027 |
| TscAI | CASTG | 8 cut(s) | 251, 612, 939, 1113, 1153, 1173, 2265, 2619 |
| TseFI | GTSAC | 2 cut(s) | 1135, 2908 |
| TseI | GCWGC | 3 cut(s) | 367, 644, 1217 |
| Tsp45I | GTSAC | 2 cut(s) | 1135, 2908 |
| TspGWI | ACGGA | 3 cut(s) | 90, 1479, 1761 |
| TspRI | CASTG | 8 cut(s) | 251, 612, 939, 1113, 1153, 1173, 2265, 2619 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1187 |
| VpaK11BI | GGWCC | 3 cut(s) | 1273, 1660, 1904 |
| VspI | ATTAAT | 2 cut(s) | 782, 1425 |
| XapI | RAATTY | 7 cut(s) | 332, 1256, 1316, 1702, 2089, 2167, 2684 |
| XbaI | TCTAGA | 1 cut(s) | 1601 |
| XceI | RCATGY | 3 cut(s) | 527, 1699, 1998 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 16, 1165, 1464 |
| XmnI | GAANNNNTTC | 2 cut(s) | 332, 2298 |
| XspI | CTAG | 4 cut(s) | 1602, 2243, 2271, 2715 |
| Zsp2I | ATGCAT | 2 cut(s) | 928, 2406 |