Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0000892

Physical Location & Seq

Genomic Coordinates Reverse (-)

34725140 .. 34731099

Gene Structure 5960 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G017510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3282 bp

ATGGACACCAATGGAAGACTGGTTGCTGGTTCTCATAACAGGAATGAGTTTGTTCTCATCAATGCTGATGAAGTTTCTAGAGTAACATCTGTTAAAGAACTGAGTGGGCAGATTTGCCAGATCTGTGGTGATGAAATAGAATTTACAGTGAATGGGGAGCCTTTTGTTGCTTGCAATGAATGTGCCTTCCCTGTTTGCAGATCTTGCTATGAGTATGAAAGAAGAGAAGGCAATCAATCATGTCCCCAATGCAAAACCAGATACAAGAGAGTGAAAGGGAGTCCAAGAGTTGAAGGAGATGAAGAAGAGGATGATATTGATGATTTGGAGAATGAGTTTGACATTGCAAGCAATGACAGGAGAGACCCTCACCAAATTGCTGCAGCGGTGCTGGCTGCTCGCCTTAATATTGGCCGGGGTTCCCAAGTCCACGGTTCGGGGATTAGTACACCGGCTGAGTTTGATACTGCATCCATGGTTTCAGAGATCCCTCTCCTGACCTATGGACAAGAGGATGTTGGGATTGCATCTGATAAACATGCGCTCATTATTCCCCCATTTATGAGTCGTGGAAAACGTGTTCATCCAATGCCATCTGCTGATGCCTCCATGTCATTTCCACCCAGGCCAATGGATCCTAAGAAAGACTTAGCTGTATATGGGTACGGAACTGTTGCATGGAAGGAGCGAATGGAGGAATGGAAGAAGAAGCAAAATGAAAAGCTTCAGGTGGTGAAGCACGAAGGAGGAAATGGTGATGGAAACAATGAGCCAATCGATTCTGATTTACCCAAGATGGATGAAGGCAGACAGCCACTGTCAAGGAAGTTGCCTATTCCTTCAAGCAAGATAAATCCATATAGAATGATCATCCTACTCCGTCTTGCGGTTCTTGGCCTTTTTTTTCACTATAGAATTCTTCATCCAGTCAACGATGCATATGGATTGTGGTTAACATCTGTAATATGTGAGATTTGGTTTGCCATGTCATGGATATTGGATCAGTTTCCCAAATGGCATCCAATTGAGAGAGAAACATACCTTGATCGATTGTCACTGAGGTATGAGAAGGAAGGGAAGACATCTGGGTTAGCTGACATAGACATCTTTGTTAGTACGGTTGATCCTATGAAGGAGCCTCCACTTATCACCGCAAACACAGTTCTGTCCATCCTTGCTGTAGACTATCCAGTCGACAAAGTTGCATGCTATGTCTCAGATGATGGTGCTGCCATGCTCACTTTTGAAGCTCTTTCAGAGACATCTGAATTTGCAAGAAAGTGGGTCCCTTTCTGCAAGAAGTTCAACATTGAACCCCGGGCCCCAGAGTGGTATTTTGCGCAAAAAGTTGACTATTTGAGAGATAAAGTAGATCCAACATTTGTCAGGGAACGTCGAGCCATGAAGAGGGATTATGAAGAGTTCAAAGTTCGGATAAATTCGTTGGTTGCCACATCACAGAAGGTTCCCGAGGAGGGTTGGACAATGCAGGATGGGTCTCCATGGCCTGGCAACAATGTACGGGATCATCCTGGAATGATTCAGGTTTTCCTTGGGCAAAATGGTTTTCGTGATATTGAAGGCAATGAGTTACCCCGTTTGGTTTATGTGTCTCGTGAGAAGAGACCGGGTTTTGATCATCACAAAAAGGCTGGCGCTATGAATGCTCTGATACGGGTGTCAGCAGTAGTCTCAAATGCTCCTTACATACTCAATGTTGACTGTGATCACTATATAAACAACAGCAAAGCACTTCGAGAAGCAATGTGCTTCATGATGGACCCCACATCAGGAAAGAAAATCTGTTACGTACAGTTTCCTCAAAGATTTGATGGGATTGATCTTCATGACAGATACTCAAATCGCAATGTTGTTTTCTTTGATATCAATATGAAAGGTTTAGATGGGATCCAAGGGCCCATTTATGTTGGAACTGGTTGTGTCTTCCGAAGGCAGGCCCTTTATGGATATGATGCTCCTGTGAAGAAGAAAGCCCCAGGGAAGACTTGTAATTGTTGGCCAAAATGGTGCTGCATGTGTTGTGGATCCAGAAAGACCAAGAAAAAAGCAAAGTCTAATGATAAAAAGAAAAAGAACAGGGAGGCTTCAAAGCAGATACATGCACTAGAGAATATTCAGGAGGGTGTTGAAGGAATAGATGATGAAAAATCATCCCTGATGCCCCAAATAAAATTTGAGAAAAAATTTGGACAGTCACCAGTTTTCATAGCTTCTTCACTTATGGAAGACAGTGGAGTTCCCAAGGGAACAAGTTCTGCATCACTCTTAAAAGAAGCCATACATGTCATTAGTTGTGGCTATGAAGATAAAACGGAATGGGGAAAGGAGGTTGGGTGGATATATGGATCTGTTACAGAAGATATCCTAACAGGTTTCAAAATGCATTGCCATGGCTGGAGGTCAGTGTATTGCATGCCCAAAAGGCCTGCATTTAAGGGTTCAGCTCCTATAAATTTGTCAGATCGGCTACACCAGGTGCTTAGATGGGCCTTGGGATCTGTTGAGATTTTATTAAGCAGGCACTGTCCCATATGGTATGGATATGGGTGTGGTTTGAAATGGTTGGAGCGCTTTTCATATATCAACTCAGTTGTATATCCTTTGACATCCATACCCTTGATTGCTTACTGTTCCTTGCCAGCTGTCTGTCTCCTTACTGGGAAATTTATTGTCCCTGAGATTAGCAACTATGCAAGTATTATTTTCATGGCCCTCTTTCTATCCATCGCGGCAACTAGTGTACTTGAGATGCAGTGGGGGCATGTCGGCATTCATGACTGGTGGAGAAATGAACAGTTTTGGGTGATTGGTGGTGCATCATCACACCTGTTTGCGCTAGTCCAGGGTCTGCTCAAGGTCTTGGGTGGAGTCAGCACAAACTTCACTGTCACATCCAAAGCAGCAGATGACGGAGAGTATTCCGAGCTATATCTCTTCAAGTGGACGGCGCTATTAATCCCTCCTATGACCTTATTGATCATAAACATAATTGGAGTCGTAGTTGGAATTTCTGATGCCATTAACAATGGTTACGAAACATGGGGTCCTCTATTTGGTAGGTTATTCTTTGCCATTTGGGTCATTGTCCATCTTTACCCATTCCTGAAAGGTATGGTGGGAAGAAATGACAGACTTCCCACAATCATTATTGTGTGGTCAATTCTTCTGGCATCCATATTCTCGCTGTTATGGGTCCGAATAAATCCATTTGTGTCCAAAGGTGGTATTGTGTTAGAAGTTTGTGGGTTGGATTGTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005887 GO:0005975 GO:0005976 GO:0006073 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009825 GO:0009832 GO:0009833 GO:0009987 GO:0010330 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0022402 GO:0030243 GO:0030244 GO:0030865 GO:0031122 GO:0031224 GO:0031226 GO:0032991 GO:0033692 GO:0034637 GO:0034645 GO:0040007 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046527 GO:0051273 GO:0051274 GO:0051301 GO:0061640 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0097435 GO:1901576 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-UDP

Protein Analysis

1093

Amino Acids

123.24

Weight (kDa)

7.07

Isoelectric Point (pI)

38.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UDP	PF14569	30 - 105	1.1e-41	Zinc-binding RING-finger
Cellulose_synt	PF03552	367 - 1081	0.0	Cellulose synthase
Glyco_trans_2_3	PF13632	783 - 886	1e-06	Glycosyl transferase family group 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21770 AT4G39350 AT5G09870 AT5G64740
fragaria_vesca	FvH4_1g23890 FvH4_1g23890 FvH4_1g23890 FvH4_1g23890 FvH4_1g23890 FvH4_2g13380 FvH4_2g13380 FvH4_2g13380 FvH4_2g13380 FvH4_2g13380 FvH4_5g26830 FvH4_5g26830 FvH4_5g26830
malus_domestica	MD00G1146900.v1.1 MD05G1029400.v1.1 MD10G1029800.v1.1 MD15G1340200.v1.1
prunus_persica	Prupe.5G000300_v2.0.a1 Prupe.5G000300_v2.0.a1 Prupe.5G000300_v2.0.a1 Prupe.6G161300_v2.0.a1 Prupe.6G161300_v2.0.a1 Prupe.8G035100_v2.0.a1
pyrus_communis	pycom01g03410 pycom01g03420 pycom01g03470 pycom05g02060 pycom05g02070 pycom15g29990
rosa_chinensis	RchiOBHm_Chr2g0119881 RchiOBHm_Chr6g0274931 RchiOBHm_Chr7g0217741
rosa_laevigata	RLG00000002442 RLG00000013507 RLG00000018496
rosa_multiflora	Rmu_sc0000202.1_g000022 Rmu_sc0001501.1_g000028 Rmu_sc0007760.1_g000002
rosa_roxburghii	Rroxscaffold_2G00124010 Rroxscaffold_3G00241720 Rroxscaffold_7G00193790
rosa_rugosa	Rorug02G0224300 Rorug02G0224400 Rorug02G0224500 Rorug06G0086400 Rorug06G0086500 Rorug07G0170900.1
rosa_samantha	Rh2AG282100 Rh2BG293400 Rh2DG288300 Rh2DG307400 Rh6AG201300 Rh6BG204900 Rh6CG206300 Rh6DG196400 Rh7AG311600 Rh7BG303500 Rh7CG330400
rosa_wichuraiana	Rw2G022520 Rw6G017510 Rw7G026440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1190
Acc16I	TGCGCA	1 cut(s)	1341
AccB7I	CCANNNNNTGG	2 cut(s)	990, 1508
AccI	GTMKAC	2 cut(s)	1182, 1194
AccII	CGCG	1 cut(s)	2750
AciI	CCGC	4 cut(s)	386, 887, 1152, 2750
AcoI	YGGCCR	2 cut(s)	412, 2018
AcsI	RAATTY	9 cut(s)	140, 915, 1268, 1438, 2192, 2204, 2473, 2684, 3027
AcuI	CTGAAG	1 cut(s)	710
AdeI	CACNNNGTG	1 cut(s)	2497
AfaI	GTAC	6 cut(s)	448, 665, 1117, 1521, 1812, 2763
AfeI	AGCGCT	1 cut(s)	2591
AflIII	ACRYGT	2 cut(s)	577, 2302
AhlI	ACTAGT	1 cut(s)	2756
AjnI	CCWGG	6 cut(s)	623, 1507, 1531, 1996, 2493, 2862
AloI	GAACNNNNNNTCC	2 cut(s)	564, 596
AluBI	AGCT	8 cut(s)	653, 724, 1094, 1250, 2231, 2465, 2663, 2947
AluI	AGCT	8 cut(s)	653, 724, 1094, 1250, 2231, 2465, 2663, 2947
Alw26I	GTCTC	8 cut(s)	357, 1219, 1253, 1503, 1617, 1618, 1696, 2675
AlwNI	CAGNNNCTG	3 cut(s)	817, 2544, 2869
Ama87I	CYCGRG	2 cut(s)	1317, 1469
Aor51HI	AGCGCT	1 cut(s)	2591
ApaI	GGGCCC	2 cut(s)	1324, 1920
ApeKI	GCWGC	6 cut(s)	380, 383, 395, 1229, 2031, 2921
ApoI	RAATTY	9 cut(s)	140, 915, 1268, 1438, 2192, 2204, 2473, 2684, 3027
AseI	ATTAAT	1 cut(s)	2975
Asp700I	GAANNNNTTC	2 cut(s)	723, 2272
AspLEI	GCGC	6 cut(s)	544, 1342, 1658, 2592, 2857, 2971
AsuC2I	CCSGG	4 cut(s)	416, 1318, 1319, 1629
AsuHPI	GGTGA	7 cut(s)	140, 362, 745, 767, 1141, 2208, 2836
AvaI	CYCGRG	2 cut(s)	1317, 1469
AvaII	GGWCC	4 cut(s)	1285, 1780, 3065, 3214
BaeGI	GKGCMC	2 cut(s)	1324, 1920
BaeI	ACNNNNGTAYC	2 cut(s)	1664, 1697
BalI	TGGCCA	1 cut(s)	2020
BamHI	GGATCC	3 cut(s)	634, 1908, 2045
BanII	GRGCYC	2 cut(s)	1324, 1920
BauI	CACGAG	1 cut(s)	1614
BbsI	GAAGAC	5 cut(s)	22, 1085, 1936, 2009, 2253
BbvI	GCAGC	6 cut(s)	367, 382, 395, 1216, 2018, 2933
BceAI	ACGGC	1 cut(s)	2982
BcgI	CGANNNNNNTGC	2 cut(s)	1184, 1218
BciT130I	CCWGG	6 cut(s)	625, 1509, 1533, 1998, 2495, 2864
BclI	TGATCA	4 cut(s)	867, 1636, 1726, 2997
BcnI	CCSGG	4 cut(s)	416, 1318, 1319, 1629
BcoDI	GTCTC	8 cut(s)	357, 1219, 1253, 1503, 1617, 1618, 1696, 2675
BcuI	ACTAGT	1 cut(s)	2756
BfaI	CTAG	4 cut(s)	78, 2126, 2757, 2858
BfmI	CTRYAG	3 cut(s)	381, 910, 1179
BfoI	RGCGCY	3 cut(s)	1659, 2593, 2972
BglI	GCCNNNNNGGC	1 cut(s)	2443
BglII	AGATCT	2 cut(s)	120, 200
BisI	GCNGC	7 cut(s)	381, 384, 396, 1230, 2032, 2751, 2922
BlsI	GCNGC	7 cut(s)	382, 385, 397, 1231, 2033, 2752, 2923
Bme18I	GGWCC	4 cut(s)	1285, 1780, 3065, 3214
BmeT110I	CYCGRG	2 cut(s)	1317, 1469
BmrI	ACTGGG	1 cut(s)	2688
BmuI	ACTGGG	1 cut(s)	2688
BpiI	GAAGAC	5 cut(s)	22, 1085, 1936, 2009, 2253
BplI	GAGNNNNNCTC	2 cut(s)	352, 384
BpmI	CTGGAG	1 cut(s)	2437
BpuEI	CTTGAG	2 cut(s)	2786, 2858
BpuMI	CCSGG	4 cut(s)	416, 1318, 1319, 1629
Bsa29I	ATCGAT	2 cut(s)	777, 1048
BsaAI	YACGTR	1 cut(s)	1810
BsaI	GGTCTC	3 cut(s)	357, 1503, 1618
BsaXI	ACNNNNNCTCC	6 cut(s)	320, 350, 2339, 2369, 3006, 3036
Bse118I	RCCGGY	1 cut(s)	451
Bse1I	ACTGG	7 cut(s)	24, 926, 1190, 1939, 2219, 2683, 2804
Bse3DI	GCAATG	7 cut(s)	181, 342, 358, 1591, 1770, 1873, 2404
BseBI	CCWGG	6 cut(s)	625, 1509, 1533, 1998, 2495, 2864
BseCI	ATCGAT	2 cut(s)	777, 1048
BseMI	GCAATG	7 cut(s)	181, 342, 358, 1591, 1770, 1873, 2404
BseMII	CTCAG	6 cut(s)	92, 447, 1049, 1230, 2622, 2688
BseNI	ACTGG	7 cut(s)	24, 926, 1190, 1939, 2219, 2683, 2804
BseRI	GAGGAG	1 cut(s)	1487
BseSI	GKGCMC	2 cut(s)	1324, 1920
BseXI	GCAGC	6 cut(s)	367, 382, 395, 1216, 2018, 2933
Bsh1236I	CGCG	1 cut(s)	2750
BshVI	ATCGAT	2 cut(s)	777, 1048
BsiHKCI	CYCGRG	2 cut(s)	1317, 1469
BsiSI	CCGG	4 cut(s)	415, 452, 1318, 1628
BslFI	GGGAC	4 cut(s)	228, 1271, 2532, 2678
BsmAI	GTCTC	8 cut(s)	357, 1219, 1253, 1503, 1617, 1618, 1696, 2675
BsmFI	GGGAC	4 cut(s)	228, 1271, 2532, 2678
BsmI	GAATGC	2 cut(s)	1669, 2790
Bso31I	GGTCTC	3 cut(s)	357, 1503, 1618
BsoBI	CYCGRG	2 cut(s)	1317, 1469
Bsp120I	GGGCCC	2 cut(s)	1320, 1916
Bsp1286I	GDGCHC	2 cut(s)	1324, 1920
Bsp19I	CCATGG	3 cut(s)	474, 1502, 2410
BspACI	CCGC	4 cut(s)	386, 887, 1152, 2750
BspCNI	CTCAG	6 cut(s)	93, 448, 1050, 1229, 2621, 2689
BspDI	ATCGAT	2 cut(s)	777, 1048
BspFNI	CGCG	1 cut(s)	2750
BspHI	TCATGA	3 cut(s)	1773, 1846, 2794
BspMAI	CTGCAG	1 cut(s)	385
BspTNI	GGTCTC	3 cut(s)	357, 1503, 1618
BsrDI	GCAATG	7 cut(s)	181, 342, 358, 1591, 1770, 1873, 2404
BsrFI	RCCGGY	1 cut(s)	451
BsrI	ACTGG	7 cut(s)	24, 926, 1190, 1939, 2219, 2683, 2804
BssAI	RCCGGY	1 cut(s)	451
BssSI	CACGAG	1 cut(s)	1614
BssT1I	CCWWGG	7 cut(s)	474, 1502, 1552, 1912, 2262, 2410, 2511
Bst2BI	CACGAG	1 cut(s)	1614
Bst2UI	CCWGG	6 cut(s)	625, 1509, 1533, 1998, 2495, 2864
Bst6I	CTCTTC	6 cut(s)	217, 300, 1400, 1413, 1616, 2960
BstAPI	GCANNNNNTGC	1 cut(s)	204
BstBAI	YACGTR	1 cut(s)	1810
BstDEI	CTNAG	9 cut(s)	101, 456, 639, 649, 1058, 1216, 2501, 2608, 2697
BstDSI	CCRYGG	4 cut(s)	430, 474, 1502, 2410
BstFNI	CGCG	1 cut(s)	2750
BstH2I	RGCGCY	3 cut(s)	1659, 2593, 2972
BstHHI	GCGC	6 cut(s)	544, 1342, 1658, 2592, 2857, 2971
BstMAI	GTCTC	8 cut(s)	357, 1219, 1253, 1503, 1617, 1618, 1696, 2675
BstMWI	GCNNNNNNNGC	5 cut(s)	204, 392, 1664, 2443, 2779
BstNI	CCWGG	6 cut(s)	625, 1509, 1533, 1998, 2495, 2864
BstNSI	RCATGY	7 cut(s)	542, 1209, 2038, 2123, 2306, 2437, 2786
BstSFI	CTRYAG	3 cut(s)	381, 910, 1179
BstSLI	GKGCMC	2 cut(s)	1324, 1920
BstSNI	TACGTA	1 cut(s)	1810
BstUI	CGCG	1 cut(s)	2750
BstV1I	GCAGC	6 cut(s)	367, 382, 395, 1216, 2018, 2933
BstV2I	GAAGAC	5 cut(s)	22, 1085, 1936, 2009, 2253
BstX2I	RGATCY	9 cut(s)	120, 200, 486, 634, 1372, 1908, 2045, 2366, 2516
BstXI	CCANNNNNNTGG	2 cut(s)	125, 631
BstYI	RGATCY	9 cut(s)	120, 200, 486, 634, 1372, 1908, 2045, 2366, 2516
Bsu15I	ATCGAT	2 cut(s)	777, 1048
BsuTUI	ATCGAT	2 cut(s)	777, 1048
BtgI	CCRYGG	4 cut(s)	430, 474, 1502, 2410
BtgZI	GCGATG	1 cut(s)	2731
BtsI	GCAGTG	1 cut(s)	2780
BtsIMutI	CAGTG	8 cut(s)	153, 815, 1055, 2257, 2430, 2542, 2780, 2904
CaiI	CAGNNNCTG	3 cut(s)	817, 2544, 2869
CciI	TCATGA	3 cut(s)	1773, 1846, 2794
CfoI	GCGC	6 cut(s)	544, 1342, 1658, 2592, 2857, 2971
Cfr10I	RCCGGY	1 cut(s)	451
Cfr9I	CCCGGG	1 cut(s)	1317
ClaI	ATCGAT	2 cut(s)	777, 1048
CsiI	ACCWGGT	1 cut(s)	2493
Csp6I	GTAC	6 cut(s)	447, 664, 1116, 1520, 1811, 2762
CviQI	GTAC	6 cut(s)	447, 664, 1116, 1520, 1811, 2762
DdeI	CTNAG	9 cut(s)	101, 456, 639, 649, 1058, 1216, 2501, 2608, 2697
DraIII	CACNNNGTG	1 cut(s)	2497
DrdI	GACNNNNNNGTC	1 cut(s)	1190
DseDI	GACNNNNNNGTC	1 cut(s)	1190
EaeI	YGGCCR	2 cut(s)	412, 2018
Eam1104I	CTCTTC	6 cut(s)	217, 300, 1400, 1413, 1616, 2960
EarI	CTCTTC	6 cut(s)	217, 300, 1400, 1413, 1616, 2960
Eco105I	TACGTA	1 cut(s)	1810
Eco130I	CCWWGG	7 cut(s)	474, 1502, 1552, 1912, 2262, 2410, 2511
Eco147I	AGGCCT	1 cut(s)	2446
Eco24I	GRGCYC	2 cut(s)	1324, 1920
Eco31I	GGTCTC	3 cut(s)	357, 1503, 1618
Eco32I	GATATC	2 cut(s)	1885, 2383
Eco47I	GGWCC	4 cut(s)	1285, 1780, 3065, 3214
Eco47III	AGCGCT	1 cut(s)	2591
Eco57I	CTGAAG	1 cut(s)	710
Eco88I	CYCGRG	2 cut(s)	1317, 1469
EcoO109I	RGGNCCY	5 cut(s)	1285, 1321, 1916, 1957, 3065
EcoRI	GAATTC	1 cut(s)	915
EcoRII	CCWGG	6 cut(s)	623, 1507, 1531, 1996, 2493, 2862
EcoRV	GATATC	2 cut(s)	1885, 2383
EcoT14I	CCWWGG	7 cut(s)	474, 1502, 1552, 1912, 2262, 2410, 2511
EcoT22I	ATGCAT	2 cut(s)	940, 2406
EcoT38I	GRGCYC	2 cut(s)	1324, 1920
ErhI	CCWWGG	7 cut(s)	474, 1502, 1552, 1912, 2262, 2410, 2511
FalI	AAGNNNNNCTT	2 cut(s)	632, 664
FaqI	GGGAC	4 cut(s)	228, 1271, 2532, 2678
FauNDI	CATATG	2 cut(s)	940, 2552
FbaI	TGATCA	4 cut(s)	867, 1636, 1726, 2997
FblI	GTMKAC	2 cut(s)	1182, 1194
Fnu4HI	GCNGC	7 cut(s)	381, 384, 396, 1230, 2032, 2751, 2922
FriOI	GRGCYC	2 cut(s)	1324, 1920
Fsp4HI	GCNGC	7 cut(s)	381, 384, 396, 1230, 2032, 2751, 2922
FspBI	CTAG	4 cut(s)	78, 2126, 2757, 2858
FspI	TGCGCA	1 cut(s)	1341
GlaI	GCGC	6 cut(s)	543, 1341, 1657, 2591, 2856, 2970
GluI	GCNGC	7 cut(s)	381, 384, 396, 1230, 2032, 2751, 2922
GsuI	CTGGAG	1 cut(s)	2437
HaeII	RGCGCY	3 cut(s)	1659, 2593, 2972
HapII	CCGG	4 cut(s)	415, 452, 1318, 1628
HhaI	GCGC	6 cut(s)	544, 1342, 1658, 2592, 2857, 2971
Hin6I	GCGC	6 cut(s)	542, 1340, 1656, 2590, 2855, 2969
HinP1I	GCGC	6 cut(s)	542, 1340, 1656, 2590, 2855, 2969
HincII	GTYRAC	5 cut(s)	931, 954, 1195, 1351, 1720
HindII	GTYRAC	5 cut(s)	931, 954, 1195, 1351, 1720
HindIII	AAGCTT	1 cut(s)	722
HinfI	GANTC	6 cut(s)	280, 565, 779, 1540, 2889, 3015
HpaI	GTTAAC	1 cut(s)	954
HpaII	CCGG	4 cut(s)	415, 452, 1318, 1628
HphI	GGTGA	7 cut(s)	140, 362, 745, 767, 1141, 2208, 2836
Hpy99I	CGWCG	1 cut(s)	1398
HpyCH4IV	ACGT	3 cut(s)	577, 1393, 1809
HpyF10VI	GCNNNNNNNGC	5 cut(s)	204, 392, 1664, 2443, 2779
HpyF3I	CTNAG	9 cut(s)	101, 456, 639, 649, 1058, 1216, 2501, 2608, 2697
HpySE526I	ACGT	3 cut(s)	577, 1393, 1809
HspAI	GCGC	6 cut(s)	542, 1340, 1656, 2590, 2855, 2969
KflI	GGGWCCC	1 cut(s)	1285
Ksp22I	TGATCA	4 cut(s)	867, 1636, 1726, 2997
KspAI	GTTAAC	1 cut(s)	954
LmnI	GCTCC	7 cut(s)	157, 685, 1135, 1705, 1981, 2470, 2587
Lsp1109I	GCAGC	6 cut(s)	367, 382, 395, 1216, 2018, 2933
MabI	ACCWGGT	1 cut(s)	2493
MaeI	CTAG	4 cut(s)	78, 2126, 2757, 2858
MaeII	ACGT	3 cut(s)	577, 1393, 1809
MaeIII	GTNAC	9 cut(s)	82, 1053, 1590, 1805, 2214, 2371, 2908, 3050, 3275
MfeI	CAATTG	1 cut(s)	1023
MflI	RGATCY	9 cut(s)	120, 200, 486, 634, 1372, 1908, 2045, 2366, 2516
MhlI	GDGCHC	2 cut(s)	1324, 1920
MlsI	TGGCCA	1 cut(s)	2020
MluNI	TGGCCA	1 cut(s)	2020
MlyI	GAGTC	4 cut(s)	289, 574, 2898, 3024
MmeI	TCCRAC	6 cut(s)	1400, 1460, 1909, 2565, 3004, 3249
Mox20I	TGGCCA	1 cut(s)	2020
Mph1103I	ATGCAT	2 cut(s)	940, 2406
MroXI	GAANNNNTTC	2 cut(s)	723, 2272
MscI	TGGCCA	1 cut(s)	2020
MseI	TTAA	8 cut(s)	93, 405, 953, 2288, 2454, 2534, 2975, 3042
MslI	CAYNNNNRTG	2 cut(s)	2409, 3170
Msp20I	TGGCCA	1 cut(s)	2020
MspA1I	CMGCKG	2 cut(s)	386, 2663
MspI	CCGG	4 cut(s)	415, 452, 1318, 1628
MunI	CAATTG	1 cut(s)	1023
Mva1269I	GAATGC	2 cut(s)	1669, 2790
MvaI	CCWGG	6 cut(s)	625, 1509, 1533, 1998, 2495, 2864
MvnI	CGCG	1 cut(s)	2750
MwoI	GCNNNNNNNGC	5 cut(s)	204, 392, 1664, 2443, 2779
NciI	CCSGG	4 cut(s)	416, 1318, 1319, 1629
NcoI	CCATGG	3 cut(s)	474, 1502, 2410
NdeI	CATATG	2 cut(s)	940, 2552
NmuCI	GTSAC	4 cut(s)	1053, 2214, 2908, 3275
NsbI	TGCGCA	1 cut(s)	1341
NsiI	ATGCAT	2 cut(s)	940, 2406
NspI	RCATGY	7 cut(s)	542, 1209, 2038, 2123, 2306, 2437, 2786
PaeI	GCATGC	2 cut(s)	1209, 2437
PagI	TCATGA	3 cut(s)	1773, 1846, 2794
PasI	CCCWGGG	1 cut(s)	1997
PceI	AGGCCT	1 cut(s)	2446
PciI	ACATGT	1 cut(s)	2302
PcsI	WCGNNNNNNNCGW	1 cut(s)	574
PctI	GAATGC	2 cut(s)	1669, 2790
PdmI	GAANNNNTTC	2 cut(s)	723, 2272
PfeI	GAWTC	2 cut(s)	779, 1540
PflMI	CCANNNNNTGG	2 cut(s)	990, 1508
PfoI	TCCNGGA	1 cut(s)	1531
PkrI	GCNGC	7 cut(s)	382, 385, 397, 1231, 2033, 2752, 2923
PleI	GAGTC	4 cut(s)	288, 573, 2897, 3023
PpsI	GAGTC	4 cut(s)	288, 573, 2897, 3023
Ppu21I	YACGTR	1 cut(s)	1810
PpuMI	RGGWCCY	2 cut(s)	1285, 3065
PscI	ACATGT	1 cut(s)	2302
PshBI	ATTAAT	1 cut(s)	2975
Psp5II	RGGWCCY	2 cut(s)	1285, 3065
Psp6I	CCWGG	6 cut(s)	623, 1507, 1531, 1996, 2493, 2862
PspGI	CCWGG	6 cut(s)	623, 1507, 1531, 1996, 2493, 2862
PspOMI	GGGCCC	2 cut(s)	1320, 1916
PspPPI	RGGWCCY	2 cut(s)	1285, 3065
PstI	CTGCAG	1 cut(s)	385
PstNI	CAGNNNCTG	3 cut(s)	817, 2544, 2869
PsuI	RGATCY	9 cut(s)	120, 200, 486, 634, 1372, 1908, 2045, 2366, 2516
PvuII	CAGCTG	1 cut(s)	2663
RsaI	GTAC	6 cut(s)	448, 665, 1117, 1521, 1812, 2763
RsaNI	GTAC	6 cut(s)	447, 664, 1116, 1520, 1811, 2762
RseI	CAYNNNNRTG	2 cut(s)	2409, 3170
SalI	GTCGAC	1 cut(s)	1193
SaqAI	TTAA	8 cut(s)	93, 405, 953, 2288, 2454, 2534, 2975, 3042
SatI	GCNGC	7 cut(s)	381, 384, 396, 1230, 2032, 2751, 2922
SchI	GAGTC	4 cut(s)	289, 574, 2898, 3024
SduI	GDGCHC	2 cut(s)	1324, 1920
SexAI	ACCWGGT	1 cut(s)	2493
SfcI	CTRYAG	3 cut(s)	381, 910, 1179
SinI	GGWCC	4 cut(s)	1285, 1780, 3065, 3214
SmaI	CCCGGG	1 cut(s)	1319
SmiMI	CAYNNNNRTG	2 cut(s)	2409, 3170
SmlI	CTYRAG	2 cut(s)	2765, 2873
SmoI	CTYRAG	2 cut(s)	2765, 2873
SnaBI	TACGTA	1 cut(s)	1810
SpeI	ACTAGT	1 cut(s)	2756
SphI	GCATGC	2 cut(s)	1209, 2437
SseBI	AGGCCT	1 cut(s)	2446
SsiI	CCGC	4 cut(s)	386, 887, 1152, 2750
SspI	AATATT	2 cut(s)	409, 2134
SspMI	CTAG	4 cut(s)	78, 2126, 2757, 2858
StuI	AGGCCT	1 cut(s)	2446
StyI	CCWWGG	7 cut(s)	474, 1502, 1552, 1912, 2262, 2410, 2511
TaiI	ACGT	3 cut(s)	580, 1396, 1812
TaqI	TCGA	5 cut(s)	777, 1048, 1194, 1396, 1756
TatI	WGTACW	2 cut(s)	446, 2761
TauI	GCSGC	1 cut(s)	2753
TfiI	GAWTC	2 cut(s)	779, 1540
Tru1I	TTAA	8 cut(s)	93, 405, 953, 2288, 2454, 2534, 2975, 3042
Tru9I	TTAA	8 cut(s)	93, 405, 953, 2288, 2454, 2534, 2975, 3042
TscAI	CASTG	8 cut(s)	153, 822, 1062, 2257, 2430, 2549, 2780, 2911
TseFI	GTSAC	4 cut(s)	1053, 2214, 2908, 3275
TseI	GCWGC	6 cut(s)	380, 383, 395, 1229, 2031, 2921
Tsp45I	GTSAC	4 cut(s)	1053, 2214, 2908, 3275
TspGWI	ACGGA	4 cut(s)	681, 869, 2348, 2946
TspMI	CCCGGG	1 cut(s)	1317
TspRI	CASTG	8 cut(s)	153, 822, 1062, 2257, 2430, 2549, 2780, 2911
Van91I	CCANNNNNTGG	2 cut(s)	990, 1508
VpaK11BI	GGWCC	4 cut(s)	1285, 1780, 3065, 3214
VspI	ATTAAT	1 cut(s)	2975
XapI	RAATTY	9 cut(s)	140, 915, 1268, 1438, 2192, 2204, 2473, 2684, 3027
XbaI	TCTAGA	1 cut(s)	77
XceI	RCATGY	7 cut(s)	542, 1209, 2038, 2123, 2306, 2437, 2786
XcmI	CCANNNNNNNNNTGG	1 cut(s)	16
XmaI	CCCGGG	1 cut(s)	1317
XmiI	GTMKAC	2 cut(s)	1182, 1194
XmnI	GAANNNNTTC	2 cut(s)	723, 2272
XspI	CTAG	4 cut(s)	78, 2126, 2757, 2858
Zsp2I	ATGCAT	2 cut(s)	940, 2406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.