Rmu_sc0000504.1_g000032

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000504.1
OG0004242
Physical Location & Seq
Forward (+)
125855 .. 130150
4296 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4296 bp
atgcctattgttcgagcacttgaatctgcttctgtaagcatcctccttaacaagttggccactcaagatgtcatagatttcttcctcaagtggaaaattgatgattccctgcgagaaaaactcaagagaaacctgcttgtcattcatgcagttctcaatcatgctgaggagaagcaagtccgggactcaaatgtcaaagcttggctggaggacgtcagagttgcagcttatgatgctgaggacatattgggtgacatcgccaatgatgctctcgaatcacaaaacagcaaacatgaggtatggaattctaagatcaaagaagggatagactttaagatcaaagacattgctaatactcttaatccctttagggaacgtgtagagtcgaaaatgataaagattatagatgtattggaagagattgcaaagcagaaagatgttcttcgtttgagagaagattctggcggtatagaatcaggttttgacggattgcccacgactccaatgttgggtgataaatctcatgtttatggtagagaatctgataagaaggagataattgctttgctgaaatcgggtaaaaatgatcgtgaggtttctataattccaattgtaggcatgggaggcattgggaagactactcttgctcagcttgtgtataatgatgccaaagtgagtagctattttaatttgagagcatgggcttgtgtctctgatgtatttgatgtgcaaaggataacaaaaacacttgttgagtccgcgacaaaatcaagttgtagcacaaacaatttggagttacttcaagaacaactgaaaatacggttgaagaacaagaaatttctttttgttctggatgatgtctggaacgacaagtatgaaagctgggataagttaagagtccccttcacagttggtgcaccagggagtaaaatcataatcacgacacgaaacaaaacagttgcatcacttatggggactgttcctacttactgtttggaggggctttcggacagtgattgttggttattattcgaacaaattgtgtttcagaacaggaatttagatgcctatccaaagttgaaagtgattggcaagaaaatagcatacaagtgtaaagggttgcctttggctgcaaaggcactaggaggtctcctgcgtgctgaaccggaactagatgagaattactggaatgatatcttgaatagcaaactttggaaactggcagacaacactgttctaccagctctaagattaagctaccatcacctccctgggcatttgaagcgctgctttgcttattgctcgatgtttcctaaggactatgaatatgagagagagatgttaatcttgttatggatggcagagggttttgtgccagagccagaaggaaatcaacggatcgaagatgtagctggaagatatgtttcagattttttatcaaggtccttcttccaacacaccagtgacaagtcacgactggtaatgcatgacctcatacatgatcttgcacaatctgtttctggcaaaacatttgttaggttggaggaaaattcagagaaccataaaaagtctacaaaggctcggcacttgtcatatacccgtggatctgaagatgtatttcagaaatttgaggcattcagtgaggtggattgtttacgaaccattctaccactagacccgttgcaaggattcaatgtgagtggcttatctgacaaggttccttgtgatatgatgccaaaattaagactcctgcgggtgctatcctttagtggttatctcatagaaaagttgccagattcaattggcaacttgaagcatctgcgatacctcaatctttcttattgtcaaatagaagagctacctgagccaacaagcagtctatacaatctgcagacattaattttattcagatgtcgatcgcttaccatgctgcctgcagacataggaaacctaaggaatttacggcatctcaatattcagggaactaggctgaaaagtatgcctttgggaatgggaagattggttaaccttcaaatactgtcagattttattgtggggaaagccatgggaaaagggattgcagaattgaaggacttgtcacatcttcgaggctcagtttctattttgtgtttgcatgatgtcggcagtgttagggatgcaattgaggccagattagaggttaagatgcaccttgatgatttagttttggaatggagcagcagtactgatggttcgcgaaatgaagagatcgagacagaggtacttgatgcactgcagcctcatgaaaacctgaagaaactcaccattaagtattatggaggtacagaatttccatattggatggggaatcctttgttcactaacatggcgtacttgcatctctatggttgcataaaatgcacatctttaccaccccttggccaactaccttctcttaaagatctcataattgaagcaatgcatggaatacagcgtgtaggaatggatttttttggggatgatgatggttccacaatttttttcccctatttagagactttgaaatttgaggacatgaaagaatgggagaaatggtcatcttcagaagatgaaaggcttaaacgatttcccagccttcgggagctgtccatatttagatgtcccaagttaacaaagtttacacatggatttgcttctctagaaaagctgcgcatcaagtactgcggagcaataactaccttctcacagaatcctgcacttgggaatttggaacctgtagaattccctagcctgcaactgcttgttcttgttggttgcagtgagcttgaaaaccttcctttttccctccctttgttgaaaaggcttgagatagatggttgtgaaaaattggctgcacttccgaggatggtggaacttagtacattgtacttgatcgattcaaatgctgaattacttggatccaagatggaatttagctcgctgacctctttgcagctaactcgtattacagatgtgaagcatctacctgaaggctttatccaacagtctgcaaaacttgaaaaattgagtcttacaaggttttgtgaccttgaatatttggcagatgaccgagttggactagcacaccttgtatcccttcaacgcttgacaatatcccattgtcctaagtttgttgctttgcctgatgagctcccacctgtacttaaatatttggatttgaaacattgtcctagcctgatgaagctgcctggtaaacttcacaaccttgagtgtcttgcagagctgcagattgaatggtgttcaaagattgaagcatttccagatgtggggttgcctgacaaactgaagcatcttgtaatccgcgaatgtggtgctctgaagactcttcctcaggagcttctctgcaataacaattttctcgagtatttagaagttcgtaaatgtccttctcttgtgtcagttcttgaagaaggaaacttgccatcaactctaacaaatatgaaattttgctattgcaaaattctgggatcactgcctgagggtttgatgcacaaagacaatatgactctaaagtacttggggatagacaactgttcctctctcatgtcctttccaaatggtgagttaccaacaagccttgagcgtcttgagattagtgaatgctctaatttccagacattgccattgagccttctcaacctcacgaatcttgagaccttagaagtaaagagctgcccccttcttcaggattttccagagggtggattgccacccaacataaagtttgtcacaatttccgaatgtgagaatctcaagtctctacctaaattctttcataaactcaaatgtcttcagaagttggagatttctggctgtcctagtctcctgtccttgccaaagcagggtctgcccagcagcttaaggctacttacagttactgattgtgaaaagcttaatcccatacatcagtggagacttcacaaactaccctctctagatgttttcaccattggtggatttcctggcctgaagtccttttcaaaagattatcctcttcctcacactttaacctctttaagcattcaaagattcccagatcttgaatccatatcggaggtgcttgagaatctcacctcacttgaaaacctgagtatcagggagtgtgataagctccaatctttgctagagactgggctaccagcgacaatttctaccttgacaattcggaattgtttgcttctaaaacctcgatgtgaaaaggacaaaggagaagattggtccaagatcaaaaacatccccttcgtgagttcacgctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1431

Amino Acids

161.93

Weight (kDa)

6.3

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 2.3e-15 Rx N-terminal domain
NB-ARC PF00931 181 - 352 1.5e-35 NB-ARC domain
WHD_DRP PF23559 436 - 503 1.1e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 850 3.6e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 820 2.1e-39 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 2690
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 2 cut(s) 2417, 3740
AccI GTMKAC 1 cut(s) 1571
AccII CGCG 3 cut(s) 761, 2236, 3342
AciI CCGC 5 cut(s) 465, 759, 1753, 2703, 3340
AclWI GGATC 5 cut(s) 1406, 1612, 2931, 2944, 3514
AcoI YGGCCR 2 cut(s) 57, 2419
AcuI CTGAAG 9 cut(s) 1629, 2312, 2565, 3027, 3344, 3377, 3707, 3815, 4028
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 9 cut(s) 2224, 2262, 2323, 2372, 2699, 2899, 2906, 3180, 3554
AfeI AGCGCT 1 cut(s) 1286
AflII CTTAAG 1 cut(s) 3898
AflIII ACRYGT 1 cut(s) 376
AjnI CCWGG 4 cut(s) 919, 1270, 3226, 4000
AleI CACNNNNGTG 1 cut(s) 1461
Alw21I GWGCWC 4 cut(s) 19, 919, 3171, 3355
Alw26I GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3686, 3801, 3866, 3946, 4160
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 5 cut(s) 1406, 1612, 2931, 2944, 3514
AlwNI CAGNNNCTG 2 cut(s) 3886, 3917
Ama87I CYCGRG 1 cut(s) 3398
Aor51HI AGCGCT 1 cut(s) 1286
AoxI GGCC 4 cut(s) 57, 2166, 2419, 4003
ApaLI GTGCAC 1 cut(s) 915
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 2 cut(s) 2811, 3294
AspLEI GCGC 2 cut(s) 1287, 2691
AspS9I GGNCC 2 cut(s) 1443, 4257
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 7 cut(s) 263, 524, 1256, 2293, 3611, 3976, 4102
AsuII TTCGAA 1 cut(s) 1032
AvaI CYCGRG 1 cut(s) 3398
AvaII GGWCC 2 cut(s) 1443, 4257
AxyI CCTNAGG 4 cut(s) 1314, 1952, 3369, 3516
BaeGI GKGCMC 1 cut(s) 919
BaeI ACNNNNGTAYC 2 cut(s) 4114, 4147
BalI TGGCCA 2 cut(s) 59, 2421
BamHI GGATCC 1 cut(s) 2936
BanII GRGCYC 1 cut(s) 3171
BarI GAAGNNNNNNTAC 2 cut(s) 3409, 3441
BbsI GAAGAC 3 cut(s) 641, 3365, 3821
Bbv12I GWGCWC 4 cut(s) 19, 919, 3171, 3355
BbvCI CCTCAGC 2 cut(s) 165, 237
BccI CCATC 9 cut(s) 1269, 1351, 2222, 2335, 2498, 2846, 2878, 2938, 3469
BceAI ACGGC 1 cut(s) 1979
BcgI CGANNNNNNTGC 6 cut(s) 2903, 2937, 2960, 2994, 3332, 3366
BciT130I CCWGG 4 cut(s) 921, 1272, 3228, 4002
BciVI GTATCC 1 cut(s) 3121
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3686, 3801, 3866, 3946, 4160
BfmI CTRYAG 5 cut(s) 1889, 1935, 2273, 2754, 3263
BfoI RGCGCY 1 cut(s) 1288
BfrI CTTAAG 1 cut(s) 3898
BfuAI ACCTGC 1 cut(s) 141
BfuI GTATCC 1 cut(s) 3121
BglII AGATCT 2 cut(s) 2440, 4075
BlpI GCTNAGC 1 cut(s) 648
BmcAI AGTACT 3 cut(s) 2224, 2699, 3554
Bme1390I CCNGG 5 cut(s) 182, 921, 1272, 3228, 4002
Bme18I GGWCC 2 cut(s) 1443, 4257
BmeT110I CYCGRG 1 cut(s) 3398
BmgT120I GGNCC 2 cut(s) 1443, 4257
BmiI GGNNCC 4 cut(s) 1719, 2509, 2751, 2938
BmrFI CCNGG 5 cut(s) 182, 921, 1272, 3228, 4002
BmrI ACTGGG 1 cut(s) 4179
BmuI ACTGGG 1 cut(s) 4179
BpiI GAAGAC 3 cut(s) 641, 3365, 3821
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 3 cut(s) 165, 237, 1863
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 1032
BpuMI CCSGG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 2913
BsaBI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 1154, 3686
BsaJI CCNNGG 7 cut(s) 920, 1270, 1271, 1600, 2064, 2416, 2879
BsaWI WCCGGW 1 cut(s) 1165
BsaXI ACNNNNNCTCC 4 cut(s) 2206, 2236, 2558, 2588
Bse1I ACTGG 5 cut(s) 1190, 1224, 1461, 1482, 4174
Bse21I CCTNAGG 4 cut(s) 1314, 1952, 3369, 3516
Bse3DI GCAATG 3 cut(s) 345, 2463, 3656
Bse8I GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseBI CCWGG 4 cut(s) 921, 1272, 3228, 4002
BseCI ATCGAT 1 cut(s) 2913
BseDI CCNNGG 7 cut(s) 920, 1270, 1271, 1600, 2064, 2416, 2879
BseGI GGATG 8 cut(s) 39, 859, 1362, 2161, 2346, 2503, 2889, 4272
BseJI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseMI GCAATG 3 cut(s) 345, 2463, 3656
BseMII CTCAG 8 cut(s) 156, 228, 662, 1854, 2127, 3383, 3507, 4118
BseNI ACTGG 5 cut(s) 1190, 1224, 1461, 1482, 4174
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 919
BseYI CCCAGC 3 cut(s) 882, 2609, 3890
BsgI GTGCAG 2 cut(s) 2718, 2856
Bsh1236I CGCG 3 cut(s) 761, 2236, 3342
Bsh1285I CGRYCG 1 cut(s) 1919
BshFI GGCC 4 cut(s) 59, 2168, 2421, 4005
BshVI ATCGAT 1 cut(s) 2913
BsiEI CGRYCG 1 cut(s) 1919
BsiHKAI GWGCWC 4 cut(s) 19, 919, 3171, 3355
BsiHKCI CYCGRG 1 cut(s) 3398
BsiSI CCGG 2 cut(s) 181, 1166
BslFI GGGAC 4 cut(s) 197, 884, 988, 2625
BsmAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3686, 3801, 3866, 3946, 4160
BsmFI GGGAC 4 cut(s) 197, 884, 988, 2625
BsmI GAATGC 3 cut(s) 1634, 3644, 4059
BsnI GGCC 4 cut(s) 59, 2168, 2421, 4005
Bso31I GGTCTC 2 cut(s) 1154, 3686
BsoBI CYCGRG 1 cut(s) 3398
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 19, 919, 3171, 3355
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 2064
Bsp68I TCGCGA 1 cut(s) 2236
BspACI CCGC 5 cut(s) 465, 759, 1753, 2703, 3340
BspANI GGCC 4 cut(s) 59, 2168, 2421, 4005
BspCNI CTCAG 8 cut(s) 157, 229, 661, 1855, 2126, 3382, 3508, 4119
BspDI ATCGAT 1 cut(s) 2913
BspFNI CGCG 3 cut(s) 761, 2236, 3342
BspHI TCATGA 1 cut(s) 2281
BspLI GGNNCC 4 cut(s) 1719, 2509, 2751, 2938
BspMAI CTGCAG 4 cut(s) 1893, 1939, 2277, 3267
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 5 cut(s) 1406, 1612, 2931, 2944, 3514
BspQI GCTCTTC 1 cut(s) 1848
BspT104I TTCGAA 1 cut(s) 1032
BspTI CTTAAG 1 cut(s) 3898
BspTNI GGTCTC 2 cut(s) 1154, 3686
BsrDI GCAATG 3 cut(s) 345, 2463, 3656
BsrI ACTGG 5 cut(s) 1190, 1224, 1461, 1482, 4174
BssECI CCNNGG 7 cut(s) 920, 1270, 1271, 1600, 2064, 2416, 2879
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 2064, 2416
Bst2UI CCWGG 4 cut(s) 921, 1272, 3228, 4002
Bst6I CTCTTC 5 cut(s) 411, 1848, 2238, 3369, 4038
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 3898
BstAPI GCANNNNNTGC 2 cut(s) 2397, 3886
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 4 cut(s) 1158, 1935, 2771, 2957
BstDSI CCRYGG 2 cut(s) 1600, 2064
BstENI CCTNNNNNAGG 1 cut(s) 3722
BstF5I GGATG 8 cut(s) 39, 859, 1362, 2161, 2346, 2503, 2889, 4272
BstFNI CGCG 3 cut(s) 761, 2236, 3342
BstH2I RGCGCY 1 cut(s) 1288
BstHHI GCGC 2 cut(s) 1287, 2691
BstMAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3686, 3801, 3866, 3946, 4160
BstMCI CGRYCG 1 cut(s) 1919
BstNI CCWGG 4 cut(s) 921, 1272, 3228, 4002
BstSCI CCNGG 5 cut(s) 180, 919, 1270, 3226, 4000
BstSFI CTRYAG 5 cut(s) 1889, 1935, 2273, 2754, 3263
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 3 cut(s) 761, 2236, 3342
BstV2I GAAGAC 3 cut(s) 641, 3365, 3821
BstX2I RGATCY 4 cut(s) 1604, 2440, 2936, 4075
BstYI RGATCY 4 cut(s) 1604, 2440, 2936, 4075
Bsu15I ATCGAT 1 cut(s) 2913
Bsu36I CCTNAGG 4 cut(s) 1314, 1952, 3369, 3516
BsuI GTATCC 1 cut(s) 3121
BsuRI GGCC 4 cut(s) 59, 2168, 2421, 4005
BsuTUI ATCGAT 1 cut(s) 2913
BtgI CCRYGG 2 cut(s) 1600, 2064
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 8 cut(s) 39, 859, 1362, 2161, 2346, 2503, 2889, 4272
BtsI GCAGTG 4 cut(s) 2152, 2270, 2803, 3509
BtsIMutI CAGTG 9 cut(s) 1018, 1230, 1468, 1645, 2152, 2270, 2803, 3509, 3953
BtuMI TCGCGA 1 cut(s) 2236
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 1158, 1935, 2771, 2957
CaiI CAGNNNCTG 2 cut(s) 3886, 3917
CciI TCATGA 1 cut(s) 2281
CfoI GCGC 2 cut(s) 1287, 2691
Cfr13I GGNCC 2 cut(s) 1443, 4257
ClaI ATCGAT 1 cut(s) 2913
CseI GACGC 1 cut(s) 3611
Csp6I GTAC 9 cut(s) 2223, 2261, 2322, 2371, 2698, 2898, 2905, 3179, 3553
CspCI CAANNNNNGTGG 2 cut(s) 1681, 1716
CviQI GTAC 9 cut(s) 2223, 2261, 2322, 2371, 2698, 2898, 2905, 3179, 3553
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 2 cut(s) 57, 2419
Eam1104I CTCTTC 5 cut(s) 411, 1848, 2238, 3369, 4038
EarI CTCTTC 5 cut(s) 411, 1848, 2238, 3369, 4038
Ecl136II GAGCTC 1 cut(s) 3169
Eco130I CCWWGG 2 cut(s) 2064, 2416
Eco24I GRGCYC 1 cut(s) 3171
Eco31I GGTCTC 2 cut(s) 1154, 3686
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 2 cut(s) 1443, 4257
Eco47III AGCGCT 1 cut(s) 1286
Eco53kI GAGCTC 1 cut(s) 3169
Eco57I CTGAAG 9 cut(s) 1629, 2312, 2565, 3027, 3344, 3377, 3707, 3815, 4028
Eco81I CCTNAGG 4 cut(s) 1314, 1952, 3369, 3516
Eco88I CYCGRG 1 cut(s) 3398
EcoICRI GAGCTC 1 cut(s) 3169
EcoNI CCTNNNNNAGG 1 cut(s) 3722
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 2 cut(s) 304, 2759
EcoRII CCWGG 4 cut(s) 919, 1270, 3226, 4000
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 2 cut(s) 2064, 2416
EcoT22I ATGCAT 2 cut(s) 1488, 2463
EcoT38I GRGCYC 1 cut(s) 3171
ErhI CCWWGG 2 cut(s) 2064, 2416
FalI AAGNNNNNCTT 6 cut(s) 426, 458, 1274, 1306, 1987, 2019
FaqI GGGAC 4 cut(s) 197, 884, 988, 2625
FauI CCCGC 1 cut(s) 1746
FblI GTMKAC 1 cut(s) 1571
FokI GGATG 8 cut(s) 26, 866, 1369, 2168, 2353, 2510, 2896, 4259
FriOI GRGCYC 1 cut(s) 3171
FspI TGCGCA 1 cut(s) 2690
GlaI GCGC 2 cut(s) 1286, 2690
GsaI CCCAGC 3 cut(s) 886, 2613, 3894
GsuI CTGGAG 1 cut(s) 227
HaeII RGCGCY 1 cut(s) 1288
HaeIII GGCC 4 cut(s) 59, 2168, 2421, 4005
HapII CCGG 2 cut(s) 181, 1166
HgaI GACGC 1 cut(s) 3611
HhaI GCGC 2 cut(s) 1287, 2691
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 2 cut(s) 1285, 2689
HinP1I GCGC 2 cut(s) 1285, 2689
HincII GTYRAC 2 cut(s) 2026, 2649
HindII GTYRAC 2 cut(s) 2026, 2649
HindIII AAGCTT 2 cut(s) 198, 3929
HpaI GTTAAC 2 cut(s) 2026, 2649
HpaII CCGG 2 cut(s) 181, 1166
HphI GGTGA 7 cut(s) 263, 524, 1256, 2293, 3611, 3976, 4102
Hpy166II GTNNAC 9 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658, 3233, 4289
Hpy8I GTNNAC 9 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658, 3233, 4289
HpyCH4IV ACGT 2 cut(s) 213, 376
HpySE526I ACGT 2 cut(s) 213, 376
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 2 cut(s) 1285, 2689
KspAI GTTAAC 2 cut(s) 2026, 2649
LguI GCTCTTC 1 cut(s) 1848
LmnI GCTCC 6 cut(s) 2214, 2620, 2705, 3174, 3373, 4155
MaeII ACGT 2 cut(s) 213, 376
MaeIII GTNAC 9 cut(s) 251, 795, 1463, 1470, 2097, 3062, 3603, 3766, 3913
MfeI CAATTG 3 cut(s) 609, 1800, 2160
MflI RGATCY 4 cut(s) 1604, 2440, 2936, 4075
MhlI GDGCHC 4 cut(s) 19, 919, 3171, 3355
MlsI TGGCCA 2 cut(s) 59, 2421
MluNI TGGCCA 2 cut(s) 59, 2421
MlyI GAGTC 9 cut(s) 179, 392, 493, 764, 906, 1740, 3055, 3355, 3538
MmeI TCCRAC 5 cut(s) 1477, 1521, 3043, 3073, 3819
Mox20I TGGCCA 2 cut(s) 59, 2421
Mph1103I ATGCAT 2 cut(s) 1488, 2463
MroXI GAANNNNTTC 2 cut(s) 2811, 3294
MscI TGGCCA 2 cut(s) 59, 2421
MslI CAYNNNNRTG 6 cut(s) 528, 1108, 1461, 2193, 2363, 2382
Msp20I TGGCCA 2 cut(s) 59, 2421
MspCI CTTAAG 1 cut(s) 3898
MspI CCGG 2 cut(s) 181, 1166
MspR9I CCNGG 5 cut(s) 182, 921, 1272, 3228, 4002
MunI CAATTG 3 cut(s) 609, 1800, 2160
Mva1269I GAATGC 3 cut(s) 1634, 3644, 4059
MvaI CCWGG 4 cut(s) 921, 1272, 3228, 4002
MvnI CGCG 3 cut(s) 761, 2236, 3342
NciI CCSGG 1 cut(s) 182
NcoI CCATGG 1 cut(s) 2064
NlaIV GGNNCC 4 cut(s) 1719, 2509, 2751, 2938
NmeAIII GCCGAG 1 cut(s) 1561
NmuCI GTSAC 6 cut(s) 251, 1463, 1470, 2097, 3062, 3766
NruI TCGCGA 1 cut(s) 2236
NsbI TGCGCA 1 cut(s) 2690
NsiI ATGCAT 2 cut(s) 1488, 2463
NspV TTCGAA 1 cut(s) 1032
OliI CACNNNNGTG 1 cut(s) 1461
PaeR7I CTCGAG 1 cut(s) 3398
PagI TCATGA 1 cut(s) 2281
PasI CCCWGGG 1 cut(s) 1271
PciSI GCTCTTC 1 cut(s) 1848
PctI GAATGC 3 cut(s) 1634, 3644, 4059
PdmI GAANNNNTTC 2 cut(s) 2811, 3294
PflMI CCANNNNNTGG 2 cut(s) 2417, 3740
PfoI TCCNGGA 1 cut(s) 180
Ple19I CGATCG 1 cut(s) 1919
PleI GAGTC 9 cut(s) 179, 391, 493, 763, 905, 1740, 3054, 3355, 3538
PpsI GAGTC 9 cut(s) 179, 391, 493, 763, 905, 1740, 3054, 3355, 3538
PpuMI RGGWCCY 1 cut(s) 1443
PshBI ATTAAT 1 cut(s) 1898
Psp124BI GAGCTC 1 cut(s) 3171
Psp5II RGGWCCY 1 cut(s) 1443
Psp6I CCWGG 4 cut(s) 919, 1270, 3226, 4000
PspFI CCCAGC 3 cut(s) 882, 2609, 3890
PspGI CCWGG 4 cut(s) 919, 1270, 3226, 4000
PspN4I GGNNCC 4 cut(s) 1719, 2509, 2751, 2938
PspPI GGNCC 2 cut(s) 1443, 4257
PspPPI RGGWCCY 1 cut(s) 1443
PsrI GAACNNNNNNTAC 2 cut(s) 2215, 2247
PstI CTGCAG 4 cut(s) 1893, 1939, 2277, 3267
PstNI CAGNNNCTG 2 cut(s) 3886, 3917
PsuI RGATCY 4 cut(s) 1604, 2440, 2936, 4075
PvuI CGATCG 1 cut(s) 1919
RruI TCGCGA 1 cut(s) 2236
RsaI GTAC 9 cut(s) 2224, 2262, 2323, 2372, 2699, 2899, 2906, 3180, 3554
RsaNI GTAC 9 cut(s) 2223, 2261, 2322, 2371, 2698, 2898, 2905, 3179, 3553
RseI CAYNNNNRTG 6 cut(s) 528, 1108, 1461, 2193, 2363, 2382
SacI GAGCTC 1 cut(s) 3171
SapI GCTCTTC 1 cut(s) 1848
Sau96I GGNCC 2 cut(s) 1443, 4257
ScaI AGTACT 3 cut(s) 2224, 2699, 3554
SchI GAGTC 9 cut(s) 179, 392, 493, 764, 906, 1740, 3055, 3355, 3538
ScrFI CCNGG 5 cut(s) 182, 921, 1272, 3228, 4002
SduI GDGCHC 4 cut(s) 19, 919, 3171, 3355
SfcI CTRYAG 5 cut(s) 1889, 1935, 2273, 2754, 3263
Sfr274I CTCGAG 1 cut(s) 3398
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 2 cut(s) 1443, 4257
SlaI CTCGAG 1 cut(s) 3398
SmiMI CAYNNNNRTG 6 cut(s) 528, 1108, 1461, 2193, 2363, 2382
SsiI CCGC 5 cut(s) 465, 759, 1753, 2703, 3340
SspI AATATT 3 cut(s) 1975, 3074, 3188
SstI GAGCTC 1 cut(s) 3171
StyD4I CCNGG 5 cut(s) 180, 919, 1270, 3226, 4000
StyI CCWWGG 2 cut(s) 2064, 2416
TaiI ACGT 2 cut(s) 216, 379
TaqII GACCGA 1 cut(s) 3102
TatI WGTACW 6 cut(s) 2222, 2697, 2897, 2904, 3178, 3552
TscAI CASTG 9 cut(s) 1018, 1237, 1468, 1645, 2152, 2277, 2803, 3516, 3953
TseFI GTSAC 6 cut(s) 251, 1463, 1470, 2097, 3062, 3766
Tsp45I GTSAC 6 cut(s) 251, 1463, 1470, 2097, 3062, 3766
TspGWI ACGGA 2 cut(s) 501, 1411
TspRI CASTG 9 cut(s) 1018, 1237, 1468, 1645, 2152, 2277, 2803, 3516, 3953
Van91I CCANNNNNTGG 2 cut(s) 2417, 3740
Vha464I CTTAAG 1 cut(s) 3898
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 1443, 4257
VspI ATTAAT 1 cut(s) 1898
XagI CCTNNNNNAGG 1 cut(s) 3722
XbaI TCTAGA 2 cut(s) 2677, 3973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 1 cut(s) 3398
XmiI GTMKAC 1 cut(s) 1571
XmnI GAANNNNTTC 2 cut(s) 2811, 3294
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 3 cut(s) 2224, 2699, 3554
Zsp2I ATGCAT 2 cut(s) 1488, 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.