Rmu_sc0000660.1_g000014

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000660.1
OG0001753
Physical Location & Seq
Reverse (-)
23060 .. 24977
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
atggcttcttctaagcaggttcaggtcacagcttgtattctatggcttctgttcctcacacttccatgttcttcttccactgaatcactctatcagcattctttggttattggtgaaaatgctactttaggattgtctcatggtatgccagtcggaaaatctcccggttctaagcctggcgcttcggtgttgtgtgaacgagttcttatctatggattgtcaaggcttaaacatccggataagtttggcaatacggtaatgaagcttctcccaactggtcggaagaattcactcgcaattttcgtcttgtcatctgtggttggtttgggatcttttctcctccgctacatcccaggactactaagttcagtactcaccgagcttggaattagtgaggatatgtataatgttttggccatatttcttctggcttttgtttttccggctggagcatcgttgggattctggacagttcgtaaaatggccttgaacgaagatggatcaattgattctagcatatctctgtttgttttgtggaccattcgcttcgtagctgttatttttctttcacagagttccagcgatcaactactggcagcaagagctataatgcccaacccatcaaggagatccacatgcctcaaaccaagagaccaccgccatagatctgcgttgcaaccatccagcactaccgccccgctccgacgaccgacgacgactcccttccacaaccataaaactcagaaaacaaggccaacgaaccagaaaaggcaacactatgcccatcccaaattagagaccgctgcctctgtgcacgatgcaaccgcatcagattctggcagaaagacgtctccacccacccgcccagccaacctgccaccaacactcaccaatcaacaaagagtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

302

Amino Acids

32.95

Weight (kDa)

10.31

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 86 - 272 6.1e-14 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 851
Acc36I ACCTGC 2 cut(s) 7, 882
AccBSI CCGCTC 1 cut(s) 700
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 7 cut(s) 343, 658, 693, 698, 801, 825, 862
AclWI GGATC 3 cut(s) 337, 508, 624
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 848
AfaI GTAC 1 cut(s) 372
AgsI TTSAA 1 cut(s) 490
AjnI CCWGG 2 cut(s) 175, 352
AluBI AGCT 5 cut(s) 32, 265, 382, 554, 605
AluI AGCT 5 cut(s) 32, 265, 382, 554, 605
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 4 cut(s) 141, 645, 791, 855
Alw44I GTGCAC 1 cut(s) 812
AlwI GGATC 3 cut(s) 337, 508, 624
AlwNI CAGNNNCTG 1 cut(s) 836
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 3 cut(s) 414, 483, 752
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 2 cut(s) 596, 803
ApoI RAATTY 1 cut(s) 286
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 537
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 3 cut(s) 125, 367, 880
AvaII GGWCC 1 cut(s) 537
BaeGI GKGCMC 1 cut(s) 816
BalI TGGCCA 1 cut(s) 416
BarI GAAGNNNNNNTAC 2 cut(s) 249, 281
Bbv12I GWGCWC 1 cut(s) 816
BbvI GCAGC 2 cut(s) 608, 790
BccI CCATC 4 cut(s) 491, 628, 688, 792
BciT130I CCWGG 2 cut(s) 177, 354
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 4 cut(s) 141, 645, 791, 855
BfaI CTAG 2 cut(s) 513, 907
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 2 cut(s) 7, 882
BglII AGATCT 1 cut(s) 665
BisI GCNGC 2 cut(s) 597, 804
BlsI GCNGC 2 cut(s) 598, 805
BmcAI AGTACT 1 cut(s) 372
Bme1390I CCNGG 3 cut(s) 165, 177, 354
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 537
BmrFI CCNGG 3 cut(s) 165, 177, 354
BmsI GCATC 3 cut(s) 461, 808, 836
BpmI CTGGAG 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 165
BsaHI GRCGYC 1 cut(s) 848
BsaI GGTCTC 2 cut(s) 645, 791
BsaJI CCNNGG 1 cut(s) 352
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 703, 733
Bse1I ACTGG 3 cut(s) 149, 280, 597
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 2 cut(s) 177, 354
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 4 cut(s) 232, 348, 680, 784
BseMII CTCAG 1 cut(s) 755
BseNI ACTGG 3 cut(s) 149, 280, 597
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 2 cut(s) 608, 790
BseYI CCCAGC 1 cut(s) 865
Bsh1285I CGRYCG 1 cut(s) 710
BshFI GGCC 3 cut(s) 416, 485, 754
BsiEI CGRYCG 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 816
BsiSI CCGG 3 cut(s) 165, 236, 443
BsmAI GTCTC 4 cut(s) 141, 645, 791, 855
BsmBI CGTCTC 1 cut(s) 855
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 3 cut(s) 416, 485, 754
Bso31I GGTCTC 2 cut(s) 645, 791
Bsp1286I GDGCHC 1 cut(s) 816
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 5 cut(s) 329, 500, 583, 629, 665
BspACI CCGC 7 cut(s) 343, 658, 693, 698, 801, 825, 862
BspANI GGCC 3 cut(s) 416, 485, 754
BspCNI CTCAG 1 cut(s) 754
BspEI TCCGGA 1 cut(s) 235
BspMI ACCTGC 2 cut(s) 7, 882
BspPI GGATC 3 cut(s) 337, 508, 624
BspTNI GGTCTC 2 cut(s) 645, 791
BsrBI CCGCTC 1 cut(s) 700
BsrI ACTGG 3 cut(s) 149, 280, 597
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 5 cut(s) 329, 500, 583, 629, 665
BssNI GRCGYC 1 cut(s) 848
Bst2UI CCWGG 2 cut(s) 177, 354
Bst4CI ACNGT 2 cut(s) 256, 472
BstACI GRCGYC 1 cut(s) 848
BstDEI CTNAG 4 cut(s) 12, 171, 362, 741
BstF5I GGATG 4 cut(s) 232, 348, 680, 784
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 5 cut(s) 332, 503, 586, 632, 668
BstMAI GTCTC 4 cut(s) 141, 645, 791, 855
BstMBI GATC 5 cut(s) 329, 500, 583, 629, 665
BstMCI CGRYCG 1 cut(s) 710
BstMWI GCNNNNNNNGC 1 cut(s) 602
BstNI CCWGG 2 cut(s) 177, 354
BstNSI RCATGY 1 cut(s) 639
BstSCI CCNGG 3 cut(s) 163, 175, 352
BstSLI GKGCMC 1 cut(s) 816
BstV1I GCAGC 2 cut(s) 608, 790
BstX2I RGATCY 3 cut(s) 329, 629, 665
BstYI RGATCY 3 cut(s) 329, 629, 665
BsuRI GGCC 3 cut(s) 416, 485, 754
BtsCI GGATG 4 cut(s) 232, 348, 680, 784
BtsIMutI CAGTG 1 cut(s) 78
BveI ACCTGC 2 cut(s) 7, 882
CaiI CAGNNNCTG 1 cut(s) 836
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 1 cut(s) 371
CviAII CATG 3 cut(s) 66, 140, 636
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 4 cut(s) 12, 171, 362, 741
DpnI GATC 5 cut(s) 331, 502, 585, 631, 667
DpnII GATC 5 cut(s) 329, 500, 583, 629, 665
EaeI YGGCCR 1 cut(s) 414
Eco31I GGTCTC 2 cut(s) 645, 791
Eco47I GGWCC 1 cut(s) 537
EcoRI GAATTC 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 175, 352
Esp3I CGTCTC 1 cut(s) 855
FaeI CATG 3 cut(s) 69, 143, 639
FatI CATG 3 cut(s) 65, 139, 635
FauI CCCGC 2 cut(s) 705, 869
Fnu4HI GCNGC 2 cut(s) 597, 804
FokI GGATG 4 cut(s) 219, 335, 667, 771
Fsp4HI GCNGC 2 cut(s) 597, 804
FspBI CTAG 2 cut(s) 513, 907
GlaI GCGC 1 cut(s) 181
GluI GCNGC 2 cut(s) 597, 804
GsaI CCCAGC 1 cut(s) 869
GsuI CTGGAG 1 cut(s) 468
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 3 cut(s) 416, 485, 754
HapII CCGG 3 cut(s) 165, 236, 443
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 1 cut(s) 848
Hin1II CATG 3 cut(s) 69, 143, 639
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 6 cut(s) 83, 462, 509, 718, 833, 903
HpaII CCGG 3 cut(s) 165, 236, 443
HphI GGTGA 3 cut(s) 125, 367, 880
Hpy166II GTNNAC 3 cut(s) 197, 537, 814
Hpy188I TCNGA 5 cut(s) 155, 282, 704, 744, 832
Hpy188III TCNNGA 2 cut(s) 236, 466
Hpy8I GTNNAC 3 cut(s) 197, 537, 814
Hpy99I CGWCG 3 cut(s) 708, 715, 718
HpyAV CCTTC 1 cut(s) 733
HpyCH4III ACNGT 2 cut(s) 256, 472
HpyCH4IV ACGT 1 cut(s) 848
HpyCH4V TGCA 3 cut(s) 676, 814, 821
HpyF10VI GCNNNNNNNGC 1 cut(s) 602
HpyF3I CTNAG 4 cut(s) 12, 171, 362, 741
HpySE526I ACGT 1 cut(s) 848
Hsp92I GRCGYC 1 cut(s) 848
Hsp92II CATG 3 cut(s) 69, 143, 639
HspAI GCGC 1 cut(s) 180
Kpn2I TCCGGA 1 cut(s) 235
Kzo9I GATC 5 cut(s) 329, 500, 583, 629, 665
LmnI GCTCC 2 cut(s) 449, 705
Lsp1109I GCAGC 2 cut(s) 608, 790
LweI GCATC 3 cut(s) 461, 808, 836
MaeI CTAG 2 cut(s) 513, 907
MaeII ACGT 1 cut(s) 848
MaeIII GTNAC 1 cut(s) 25
MalI GATC 5 cut(s) 331, 502, 585, 631, 667
MbiI CCGCTC 1 cut(s) 700
MboI GATC 5 cut(s) 329, 500, 583, 629, 665
MboII GAAGA 5 cut(s) 63, 66, 295, 416, 506
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 3 cut(s) 329, 629, 665
MhlI GDGCHC 1 cut(s) 816
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 5 cut(s) 286, 297, 387, 504, 791
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 1 cut(s) 712
MmeI TCCRAC 3 cut(s) 133, 260, 727
MnlI CCTC 5 cut(s) 65, 350, 388, 650, 817
Mox20I TGGCCA 1 cut(s) 416
MroI TCCGGA 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 1 cut(s) 416
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 64
Msp20I TGGCCA 1 cut(s) 416
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 3 cut(s) 165, 236, 443
MspR9I CCNGG 3 cut(s) 165, 177, 354
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 177, 354
MwoI GCNNNNNNNGC 1 cut(s) 602
NciI CCSGG 1 cut(s) 165
NdeII GATC 5 cut(s) 329, 500, 583, 629, 665
NlaIII CATG 3 cut(s) 69, 143, 639
NmuCI GTSAC 1 cut(s) 25
NspI RCATGY 1 cut(s) 639
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 4 cut(s) 83, 462, 509, 833
PkrI GCNGC 2 cut(s) 598, 805
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
Psp6I CCWGG 2 cut(s) 175, 352
PspFI CCCAGC 1 cut(s) 865
PspGI CCWGG 2 cut(s) 175, 352
PspPI GGNCC 1 cut(s) 537
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 3 cut(s) 329, 629, 665
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 597, 804
Sau3AI GATC 5 cut(s) 329, 500, 583, 629, 665
Sau96I GGNCC 1 cut(s) 537
ScaI AGTACT 1 cut(s) 372
SchI GAGTC 1 cut(s) 712
ScrFI CCNGG 3 cut(s) 165, 177, 354
SduI GDGCHC 1 cut(s) 816
SetI ASST 9 cut(s) 21, 27, 34, 267, 384, 556, 607, 851, 876
SfaNI GCATC 3 cut(s) 461, 808, 836
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 5 cut(s) 286, 297, 387, 504, 791
SsiI CCGC 7 cut(s) 343, 658, 693, 698, 801, 825, 862
SspMI CTAG 2 cut(s) 513, 907
StyD4I CCNGG 3 cut(s) 163, 175, 352
TaaI ACNGT 2 cut(s) 256, 472
TaiI ACGT 1 cut(s) 851
TaqII GACCGA 1 cut(s) 724
TasI AATT 5 cut(s) 286, 297, 387, 504, 791
TatI WGTACW 1 cut(s) 370
TfiI GAWTC 4 cut(s) 83, 462, 509, 833
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 2 cut(s) 596, 803
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 1 cut(s) 275
TspRI CASTG 1 cut(s) 85
VneI GTGCAC 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 639
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 2 cut(s) 513, 907
ZraI GACGTC 1 cut(s) 849
ZrmI AGTACT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.