Rmu_sc0000665.1_g000025

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000665.1
OG0002871
Physical Location & Seq
Reverse (-)
110015 .. 111907
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
atgggttcttgcagcagaagaagagaggatgaaggctctgaaaatccaggcttctacaagtgtgttgattactctgttctttcaacagggaagctttacattggacatgcaaagtttaatgttgaaatttatgggctcaatcgctgcaataagatgtcaaaggctaggaaaagtgacaggcttcttgatgattttgatgtgattattgtgtactctgattcagattctgaaattgagtttcctagggaaccggtttgtcgaagaaaattgtcaaggagaggtagacctcgtaatttagccaccgtaaaccgttctggaaatccctgcttcagtgtccagatgtgccagactcatgtgaccaagggaccattgctcacaccaaggagcttcatgaggaacacacacaagccaaagaaagttgagcttcaagttggagacatggtctgggaggtgaactggatccatccttaccagaagtctagtaggttcagtggaggctggctacgttttgtgagccataacggattgcaagagggagatttatgtgactttgagctgatatcgaagacaaatgaggttgcggtgatgaacgtttctattaggaaaatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

203

Amino Acids

23.37

Weight (kDa)

9.39

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 112 - 193 1.1e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 283
AciI CCGC 1 cut(s) 581
AclI AACGTT 1 cut(s) 591
AclWI GGATC 2 cut(s) 454, 467
AcsI RAATTY 1 cut(s) 126
AcuI CTGAAG 1 cut(s) 313
AfaI GTAC 1 cut(s) 212
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 3 cut(s) 84, 125, 428
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 4 cut(s) 94, 387, 424, 556
AluI AGCT 4 cut(s) 94, 387, 424, 556
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 2 cut(s) 454, 467
AlwNI CAGNNNCTG 1 cut(s) 227
ApeKI GCWGC 2 cut(s) 12, 144
ApoI RAATTY 1 cut(s) 126
AsiGI ACCGGT 1 cut(s) 250
AspA2I CCTAGG 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 2 cut(s) 463, 595
AvaII GGWCC 1 cut(s) 365
AvrII CCTAGG 1 cut(s) 242
BamHI GGATCC 1 cut(s) 459
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 572
BbvI GCAGC 2 cut(s) 24, 131
BccI CCATC 1 cut(s) 471
BciT130I CCWGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 3 cut(s) 165, 243, 480
BisI GCNGC 2 cut(s) 13, 145
BlnI CCTAGG 1 cut(s) 242
BlsI GCNGC 2 cut(s) 14, 146
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 3 cut(s) 249, 366, 461
BmrFI CCNGG 1 cut(s) 48
BpiI GAAGAC 1 cut(s) 572
BsaJI CCNNGG 3 cut(s) 242, 360, 380
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 461
Bse3DI GCAATG 1 cut(s) 368
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 242, 360, 380
BseGI GGATG 2 cut(s) 34, 463
BseMI GCAATG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 461
BseXI GCAGC 2 cut(s) 24, 131
BshTI ACCGGT 1 cut(s) 250
BsiSI CCGG 1 cut(s) 251
BslFI GGGAC 1 cut(s) 378
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 459
BspACI CCGC 1 cut(s) 581
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 3 cut(s) 249, 366, 461
BspPI GGATC 2 cut(s) 454, 467
BsrDI GCAATG 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 1 cut(s) 461
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 242, 360, 380
BssMI GATC 1 cut(s) 459
BssT1I CCWWGG 3 cut(s) 242, 360, 380
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 2 cut(s) 304, 311
Bst6I CTCTTC 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 500
BstF5I GGATG 2 cut(s) 34, 463
BstKTI GATC 1 cut(s) 462
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 1 cut(s) 459
BstNI CCWGG 1 cut(s) 48
BstNSI RCATGY 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 24, 131
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BtsCI GGATG 2 cut(s) 34, 463
BtsIMutI CAGTG 2 cut(s) 337, 496
Cac8I GCNNGC 1 cut(s) 500
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 1 cut(s) 211
CspAI ACCGGT 1 cut(s) 250
CviAII CATG 4 cut(s) 107, 353, 391, 439
CviQI GTAC 1 cut(s) 211
DpnI GATC 1 cut(s) 461
DpnII GATC 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 3 cut(s) 242, 360, 380
Eco24I GRGCYC 1 cut(s) 138
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 46
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 3 cut(s) 242, 360, 380
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 3 cut(s) 242, 360, 380
FaeI CATG 4 cut(s) 110, 356, 394, 442
FaiI YATR 7 cut(s) 108, 132, 354, 392, 440, 519, 544
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FaqI GGGAC 1 cut(s) 378
FatI CATG 4 cut(s) 106, 352, 390, 438
FblI GTMKAC 1 cut(s) 283
Fnu4HI GCNGC 2 cut(s) 13, 145
FokI GGATG 2 cut(s) 41, 450
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 13, 145
FspBI CTAG 3 cut(s) 165, 243, 480
GluI GCNGC 2 cut(s) 13, 145
HapII CCGG 1 cut(s) 251
Hin1II CATG 4 cut(s) 110, 356, 394, 442
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 3 cut(s) 218, 224, 349
HpaII CCGG 1 cut(s) 251
HphI GGTGA 2 cut(s) 463, 595
Hpy166II GTNNAC 4 cut(s) 211, 284, 307, 454
Hpy188I TCNGA 4 cut(s) 40, 217, 223, 229
Hpy188III TCNNGA 4 cut(s) 185, 315, 337, 391
Hpy8I GTNNAC 4 cut(s) 211, 284, 307, 454
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 2 cut(s) 304, 311
HpyCH4IV ACGT 2 cut(s) 505, 591
HpyCH4V TGCA 4 cut(s) 12, 110, 147, 529
HpySE526I ACGT 2 cut(s) 505, 591
Hsp92II CATG 4 cut(s) 110, 356, 394, 442
Kzo9I GATC 1 cut(s) 459
LmnI GCTCC 1 cut(s) 384
Lsp1109I GCAGC 2 cut(s) 24, 131
MaeI CTAG 3 cut(s) 165, 243, 480
MaeII ACGT 2 cut(s) 505, 591
MaeIII GTNAC 3 cut(s) 173, 355, 545
MalI GATC 1 cut(s) 461
MboI GATC 1 cut(s) 459
MboII GAAGA 4 cut(s) 30, 33, 273, 577
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 4 cut(s) 126, 231, 266, 292
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 8 cut(s) 19, 272, 297, 387, 442, 488, 526, 568
MseI TTAA 1 cut(s) 117
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 48
MvaI CCWGG 1 cut(s) 48
NdeII GATC 1 cut(s) 459
NlaIII CATG 4 cut(s) 110, 356, 394, 442
NlaIV GGNNCC 3 cut(s) 249, 366, 461
NmuCI GTSAC 3 cut(s) 173, 355, 545
NspI RCATGY 1 cut(s) 110
PagI TCATGA 1 cut(s) 390
PfeI GAWTC 2 cut(s) 218, 224
PflFI GACNNNGTC 1 cut(s) 440
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 2 cut(s) 14, 146
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
Psp1406I AACGTT 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 3 cut(s) 249, 366, 461
PspPI GGNCC 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 1 cut(s) 459
PsyI GACNNNGTC 1 cut(s) 440
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 2 cut(s) 13, 145
Sau3AI GATC 1 cut(s) 459
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 1 cut(s) 138
SinI GGWCC 1 cut(s) 365
Sse9I AATT 4 cut(s) 126, 231, 266, 292
SsiI CCGC 1 cut(s) 581
SspMI CTAG 3 cut(s) 165, 243, 480
StyD4I CCNGG 1 cut(s) 46
StyI CCWWGG 3 cut(s) 242, 360, 380
TaaI ACNGT 2 cut(s) 304, 311
TaiI ACGT 2 cut(s) 508, 594
TaqI TCGA 2 cut(s) 259, 563
TasI AATT 4 cut(s) 126, 231, 266, 292
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 2 cut(s) 218, 224
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 2 cut(s) 337, 496
TseFI GTSAC 3 cut(s) 173, 355, 545
TseI GCWGC 2 cut(s) 12, 144
Tsp45I GTSAC 3 cut(s) 173, 355, 545
TspDTI ATGAA 3 cut(s) 45, 379, 602
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 2 cut(s) 337, 496
Tth111I GACNNNGTC 1 cut(s) 440
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 1 cut(s) 126
XceI RCATGY 1 cut(s) 110
XmaJI CCTAGG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 283
XspI CTAG 3 cut(s) 165, 243, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.