Rmu_sc0000814.1_g000040

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000814.1
OG0002520
Physical Location & Seq
Forward (+)
133366 .. 136768
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
atggcttccgaaggaacaagtcgctccctgcaagacacacccagttgggctctggcaacagtttgcttcgtcttcatctttctctctcttttcatagagcacttgatccatctcctttcccaatttctcaaaaagcatagaaagactgcattgttcgaggccgtagagaagctcaaatcagtgcttatttttctgggtttcatgtccctaatactgacagtaacacagagatggatagccaatatttgcatacccacagaagttgcaaatactatgcttccttgcagaaaatcagtatcaactaaaacaaccaaagcagttggtttcgatcgcatgtggagcaccggggcatctacagcttttgaagtaatacaaagaagactagcagaaacagaagatagtagtactactagttccacttctaccacttctgtggatgtggatcggtgcgaatcccaggggaagacctcatttatatctcaagggggactaaatcagctcaacaacttcatctttgttctggcaattatgcagatcgtgtacagtgttctgacaatggccttaggaagagccaagatgaagcgttgggaagcgtgggaaagagaaactcagacggtggaataccaagtcgcaaatgatcctaaccgattcaggtttacaaggcagacgacatttgctcgtagacacatgaattcttgtacacgaacacctattcaactgtggattaagtgcttcttcagacaattcttccactcagttgaaaaaattgattatctcacattacgccatggcttcatttct
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

267

Amino Acids

30.67

Weight (kDa)

9.66

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 266 1.4e-91 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 682
AclWI GGATC 3 cut(s) 100, 450, 632
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 1 cut(s) 721
AfaI GTAC 3 cut(s) 406, 542, 700
AgsI TTSAA 3 cut(s) 365, 716, 761
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 1 cut(s) 456
AleI CACNNNNGTG 1 cut(s) 431
AluBI AGCT 3 cut(s) 172, 359, 499
AluI AGCT 3 cut(s) 172, 359, 499
Alw21I GWGCWC 2 cut(s) 102, 344
AlwI GGATC 3 cut(s) 100, 450, 632
AoxI GGCC 2 cut(s) 159, 558
ApoI RAATTY 1 cut(s) 691
AsuC2I CCSGG 1 cut(s) 346
AxyI CCTNAGG 1 cut(s) 562
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 3 cut(s) 64, 385, 470
Bbv12I GWGCWC 2 cut(s) 102, 344
BccI CCATC 2 cut(s) 117, 225
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 458
BcnI CCSGG 1 cut(s) 346
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 2 cut(s) 383, 411
BfmI CTRYAG 1 cut(s) 354
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 2 cut(s) 346, 458
BmrFI CCNGG 2 cut(s) 346, 458
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 359
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 3 cut(s) 64, 385, 470
BpuEI CTTGAG 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 4 cut(s) 345, 456, 457, 787
Bse1I ACTGG 1 cut(s) 42
Bse21I CCTNAGG 1 cut(s) 562
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 4 cut(s) 345, 456, 457, 787
BseGI GGATG 1 cut(s) 442
BseJI GATNNNNATC 1 cut(s) 441
BseMII CTCAG 2 cut(s) 623, 768
BseNI ACTGG 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 2 cut(s) 161, 560
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 2 cut(s) 102, 344
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 2 cut(s) 190, 501
BsmFI GGGAC 2 cut(s) 190, 501
BsnI GGCC 2 cut(s) 161, 560
Bsp1286I GDGCHC 3 cut(s) 52, 102, 344
Bsp1407I TGTACA 2 cut(s) 540, 698
Bsp143I GATC 5 cut(s) 105, 328, 442, 534, 637
Bsp19I CCATGG 1 cut(s) 787
BspANI GGCC 2 cut(s) 161, 560
BspCNI CTCAG 2 cut(s) 622, 767
BspPI GGATC 3 cut(s) 100, 450, 632
BspQI GCTCTTC 1 cut(s) 562
BsrGI TGTACA 2 cut(s) 540, 698
BsrI ACTGG 1 cut(s) 42
BssECI CCNNGG 4 cut(s) 345, 456, 457, 787
BssMI GATC 5 cut(s) 105, 328, 442, 534, 637
BssT1I CCWWGG 1 cut(s) 787
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 5 cut(s) 61, 220, 545, 616, 720
Bst6I CTCTTC 1 cut(s) 562
BstAUI TGTACA 2 cut(s) 540, 698
BstDEI CTNAG 3 cut(s) 562, 609, 754
BstDSI CCRYGG 1 cut(s) 787
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 5 cut(s) 108, 331, 445, 537, 640
BstMBI GATC 5 cut(s) 105, 328, 442, 534, 637
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 339, 356
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 1 cut(s) 337
BstSCI CCNGG 2 cut(s) 344, 456
BstSFI CTRYAG 1 cut(s) 354
BstV2I GAAGAC 3 cut(s) 64, 385, 470
BstXI CCANNNNNNTGG 1 cut(s) 433
Bsu36I CCTNAGG 1 cut(s) 562
BsuRI GGCC 2 cut(s) 161, 560
BtgI CCRYGG 1 cut(s) 787
BtsCI GGATG 1 cut(s) 442
BtsIMutI CAGTG 2 cut(s) 186, 550
Csp6I GTAC 3 cut(s) 405, 541, 699
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 4 cut(s) 202, 334, 688, 788
CviQI GTAC 3 cut(s) 405, 541, 699
DdeI CTNAG 3 cut(s) 562, 609, 754
DpnI GATC 5 cut(s) 107, 330, 444, 536, 639
DpnII GATC 5 cut(s) 105, 328, 442, 534, 637
Eam1104I CTCTTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 562
Eco130I CCWWGG 1 cut(s) 787
Eco24I GRGCYC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 721
Eco81I CCTNAGG 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 787
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 787
FaeI CATG 4 cut(s) 205, 337, 691, 791
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 2 cut(s) 190, 501
FatI CATG 4 cut(s) 201, 333, 687, 787
FblI GTMKAC 1 cut(s) 682
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 52
FspBI CTAG 2 cut(s) 383, 411
HaeIII GGCC 2 cut(s) 161, 560
HapII CCGG 1 cut(s) 345
Hin1II CATG 4 cut(s) 205, 337, 691, 791
HinfI GANTC 2 cut(s) 452, 648
HpaII CCGG 1 cut(s) 345
Hpy166II GTNNAC 4 cut(s) 541, 657, 683, 701
Hpy188I TCNGA 4 cut(s) 10, 552, 612, 740
Hpy8I GTNNAC 4 cut(s) 541, 657, 683, 701
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 5 cut(s) 61, 220, 545, 616, 720
HpyCH4V TGCA 6 cut(s) 31, 149, 249, 266, 285, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 339, 356
HpyF3I CTNAG 3 cut(s) 562, 609, 754
Hsp92II CATG 4 cut(s) 205, 337, 691, 791
Kzo9I GATC 5 cut(s) 105, 328, 442, 534, 637
LguI GCTCTTC 1 cut(s) 562
LmnI GCTCC 2 cut(s) 29, 339
LpnPI CCDG 9 cut(s) 38, 41, 55, 179, 358, 443, 470, 506, 637
LweI GCATC 1 cut(s) 359
MaeI CTAG 2 cut(s) 383, 411
MaeIII GTNAC 1 cut(s) 220
MalI GATC 5 cut(s) 107, 330, 444, 536, 639
MboI GATC 5 cut(s) 105, 328, 442, 534, 637
MboII GAAGA 7 cut(s) 64, 390, 407, 475, 579, 727, 739
MhlI GDGCHC 3 cut(s) 52, 102, 344
MluCI AATT 5 cut(s) 122, 525, 691, 743, 765
MnlI CCTC 2 cut(s) 151, 478
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 2 cut(s) 229, 431
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 346, 458
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 2 cut(s) 339, 356
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 787
NdeII GATC 5 cut(s) 105, 328, 442, 534, 637
NlaIII CATG 4 cut(s) 205, 337, 691, 791
NspI RCATGY 1 cut(s) 337
OliI CACNNNNGTG 1 cut(s) 431
PasI CCCWGGG 1 cut(s) 457
PciSI GCTCTTC 1 cut(s) 562
PfeI GAWTC 2 cut(s) 452, 648
Ple19I CGATCG 1 cut(s) 331
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 3 cut(s) 406, 542, 700
RsaNI GTAC 3 cut(s) 405, 541, 699
RseI CAYNNNNRTG 2 cut(s) 229, 431
SapI GCTCTTC 1 cut(s) 562
SaqAI TTAA 1 cut(s) 726
Sau3AI GATC 5 cut(s) 105, 328, 442, 534, 637
ScaI AGTACT 1 cut(s) 406
ScrFI CCNGG 2 cut(s) 346, 458
SduI GDGCHC 3 cut(s) 52, 102, 344
SetI ASST 6 cut(s) 174, 361, 470, 501, 656, 712
SfaNI GCATC 1 cut(s) 359
SfcI CTRYAG 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 229, 431
SmlI CTYRAG 1 cut(s) 480
SmoI CTYRAG 1 cut(s) 480
SpeI ACTAGT 1 cut(s) 410
Sse9I AATT 5 cut(s) 122, 525, 691, 743, 765
SspI AATATT 1 cut(s) 244
SspMI CTAG 2 cut(s) 383, 411
StyD4I CCNGG 2 cut(s) 344, 456
StyI CCWWGG 1 cut(s) 787
TaaI ACNGT 5 cut(s) 61, 220, 545, 616, 720
TaqI TCGA 2 cut(s) 156, 327
TasI AATT 5 cut(s) 122, 525, 691, 743, 765
TatI WGTACW 3 cut(s) 404, 540, 698
TfiI GAWTC 2 cut(s) 452, 648
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 2 cut(s) 186, 550
TspDTI ATGAA 7 cut(s) 64, 82, 190, 499, 593, 704, 784
TspRI CASTG 2 cut(s) 186, 550
XapI RAATTY 1 cut(s) 691
XceI RCATGY 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 682
XspI CTAG 2 cut(s) 383, 411
ZrmI AGTACT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.