Rmu_sc0000920.1_g000006

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000920.1
OG0004190
Physical Location & Seq
Forward (+)
20540 .. 22273
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
atggtgatgaacaatatcaagttatccctcaatggtcctggaggtattgcgagttcttcctgcgaaaatgccattggcgtctcttacggcacacaaaaccctccttcttgcataaaatctttcagttttgacttcgtcacaaatgcaagcgtcgctcacttgagatcatacaacagcaaaaatgcccatatcaaggtttatggatgcaacaaaatggacttcaccaaacttagattgtcagctccaggtgatagcccaaacaccgatggcataaaaatcggaaactcctaccgcatcagaatctcacgcgcggttattggcaccggcgatgactgcattgcgatattgtcaggaagtagggaaatccacatttctaaaattttttgcggtcctgggcatggaatcagcattggaagtctcggagggtatgataatgaatacgatgtggaaggagtatatgtgaaagatagcggtttaaaggggactaccaatggtctaagaatcaaaacatgggcttattctcatcccttgaaggtttcaaagattagatacgaaaacattgtgatgactgatgtcacgaatccaattattatcgatcaaaattattgccccagccctccctgcaatcaacagtacgagacagagtccacgctaccataccacaactcggtagatatttattgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

228

Amino Acids

24.79

Weight (kDa)

8.06

Isoelectric Point (pI)

29.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 30 - 212 6.2e-64 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccII CGCG 2 cut(s) 309, 311
AciI CCGC 4 cut(s) 292, 311, 387, 471
AcsI RAATTY 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 3 cut(s) 193, 398, 667
AgsI TTSAA 2 cut(s) 532, 540
AjnI CCWGG 3 cut(s) 37, 244, 391
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Alw26I GTCTC 3 cut(s) 85, 422, 632
ApoI RAATTY 1 cut(s) 378
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 2 cut(s) 35, 389
AsuHPI GGTGA 3 cut(s) 16, 214, 260
AvaII GGWCC 2 cut(s) 35, 389
BanI GGYRCC 1 cut(s) 320
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 3 cut(s) 39, 246, 393
BcoDI GTCTC 3 cut(s) 85, 422, 632
Bme1390I CCNGG 3 cut(s) 39, 246, 393
Bme18I GGWCC 2 cut(s) 35, 389
BmgT120I GGNCC 2 cut(s) 35, 389
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 3 cut(s) 39, 246, 393
BmsI GCATC 2 cut(s) 194, 303
BoxI GACNNNNGTC 1 cut(s) 572
BpmI CTGGAG 2 cut(s) 60, 228
BpuEI CTTGAG 1 cut(s) 181
Bsa29I ATCGAT 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 398, 667
Bse118I RCCGGY 1 cut(s) 323
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 3 cut(s) 39, 246, 393
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 2 cut(s) 209, 523
BseLI CCNNNNNNNGG 3 cut(s) 193, 398, 667
BseMI GCAATG 1 cut(s) 336
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 2 cut(s) 309, 311
BshNI GGYRCC 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 594
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 3 cut(s) 193, 398, 667
BsmAI GTCTC 3 cut(s) 85, 422, 632
BsmBI CGTCTC 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 496
Bsp143I GATC 2 cut(s) 164, 595
BspACI CCGC 4 cut(s) 292, 311, 387, 471
BspDI ATCGAT 1 cut(s) 594
BspFNI CGCG 2 cut(s) 309, 311
BspLI GGNNCC 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 323
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 2 cut(s) 164, 595
BssNI GRCGYC 1 cut(s) 78
Bst2UI CCWGG 3 cut(s) 39, 246, 393
Bst4CI ACNGT 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 2 cut(s) 230, 497
BstF5I GGATG 2 cut(s) 209, 523
BstFNI CGCG 2 cut(s) 309, 311
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 2 cut(s) 167, 598
BstMAI GTCTC 3 cut(s) 85, 422, 632
BstMBI GATC 2 cut(s) 164, 595
BstMWI GCNNNNNNNGC 3 cut(s) 152, 333, 621
BstNI CCWGG 3 cut(s) 39, 246, 393
BstPAI GACNNNNGTC 1 cut(s) 572
BstSCI CCNGG 3 cut(s) 37, 244, 391
BstUI CGCG 2 cut(s) 309, 311
Bsu15I ATCGAT 1 cut(s) 594
BsuTUI ATCGAT 1 cut(s) 594
BtgZI GCGATG 1 cut(s) 342
BtsCI GGATG 2 cut(s) 209, 523
Cac8I GCNNGC 1 cut(s) 148
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 2 cut(s) 35, 389
ClaI ATCGAT 1 cut(s) 594
CseI GACGC 2 cut(s) 67, 139
Csp6I GTAC 1 cut(s) 634
CviAII CATG 2 cut(s) 398, 510
CviJI RGCY 4 cut(s) 242, 255, 515, 615
CviKI_1 RGCY 4 cut(s) 242, 255, 515, 615
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 2 cut(s) 230, 497
DpnI GATC 2 cut(s) 166, 597
DpnII GATC 2 cut(s) 164, 595
DraI TTTAAA 1 cut(s) 477
Eco47I GGWCC 2 cut(s) 35, 389
EcoRII CCWGG 3 cut(s) 37, 244, 391
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 2 cut(s) 401, 513
FaqI GGGAC 1 cut(s) 496
FatI CATG 2 cut(s) 397, 509
FokI GGATG 2 cut(s) 216, 510
GlaI GCGC 1 cut(s) 310
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 2 cut(s) 60, 228
HapII CCGG 1 cut(s) 324
HgaI GACGC 2 cut(s) 67, 139
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 2 cut(s) 401, 513
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HinfI GANTC 5 cut(s) 300, 402, 501, 580, 644
HpaII CCGG 1 cut(s) 324
HphI GGTGA 3 cut(s) 16, 214, 260
Hpy166II GTNNAC 1 cut(s) 648
Hpy188I TCNGA 3 cut(s) 281, 299, 422
Hpy188III TCNNGA 2 cut(s) 351, 577
Hpy8I GTNNAC 1 cut(s) 648
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 114, 443, 526
HpyCH4III ACNGT 1 cut(s) 633
HpyCH4V TGCA 5 cut(s) 111, 146, 207, 336, 624
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 333, 621
HpyF3I CTNAG 2 cut(s) 230, 497
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 2 cut(s) 401, 513
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 2 cut(s) 164, 595
LmnI GCTCC 1 cut(s) 247
LweI GCATC 2 cut(s) 194, 303
MaeIII GTNAC 2 cut(s) 136, 574
MalI GATC 2 cut(s) 166, 597
MboI GATC 2 cut(s) 164, 595
MboII GAAGA 1 cut(s) 48
MluCI AATT 3 cut(s) 378, 585, 601
MlyI GAGTC 1 cut(s) 653
MnlI CCTC 5 cut(s) 35, 38, 111, 416, 627
MseI TTAA 1 cut(s) 476
MslI CAYNNNNRTG 1 cut(s) 563
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 3 cut(s) 39, 246, 393
MvaI CCWGG 3 cut(s) 39, 246, 393
MvnI CGCG 2 cut(s) 309, 311
MwoI GCNNNNNNNGC 3 cut(s) 152, 333, 621
NdeII GATC 2 cut(s) 164, 595
NlaIII CATG 2 cut(s) 401, 513
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 136, 574
PfeI GAWTC 4 cut(s) 300, 402, 501, 580
PflFI GACNNNGTC 2 cut(s) 134, 643
PfoI TCCNGGA 1 cut(s) 37
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
PshAI GACNNNNGTC 1 cut(s) 572
Psp6I CCWGG 3 cut(s) 37, 244, 391
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 37, 244, 391
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 2 cut(s) 35, 389
PsrI GAACNNNNNNTAC 2 cut(s) 37, 69
PsyI GACNNNGTC 2 cut(s) 134, 643
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 563
SaqAI TTAA 1 cut(s) 476
Sau3AI GATC 2 cut(s) 164, 595
Sau96I GGNCC 2 cut(s) 35, 389
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 3 cut(s) 39, 246, 393
SetI ASST 5 cut(s) 46, 198, 244, 250, 537
SfaNI GCATC 2 cut(s) 194, 303
SgrAI CRCCGGYG 1 cut(s) 323
SinI GGWCC 2 cut(s) 35, 389
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 3 cut(s) 378, 585, 601
SsiI CCGC 4 cut(s) 292, 311, 387, 471
StyD4I CCNGG 3 cut(s) 37, 244, 391
TaaI ACNGT 1 cut(s) 633
TaqI TCGA 1 cut(s) 594
TasI AATT 3 cut(s) 378, 585, 601
TfiI GAWTC 4 cut(s) 300, 402, 501, 580
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TseFI GTSAC 2 cut(s) 136, 574
Tsp45I GTSAC 2 cut(s) 136, 574
TspDTI ATGAA 2 cut(s) 23, 450
Tth111I GACNNNGTC 2 cut(s) 134, 643
VpaK11BI GGWCC 2 cut(s) 35, 389
XapI RAATTY 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.