Rmu_sc0000951.1_g000010

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000951.1
OG0005521
Physical Location & Seq
Reverse (-)
56534 .. 58435
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 807 bp
atggaagagtgcaagaaactcggccttgctagaggtattggagttagtaacttcacatgcagtatgctcgaggagcttctttccttcgccgaaatccctccggccgtcaaccaagcaaagggtgttcatgtcacggcttactctccgctcggagcagctagggctaaatggggtgacaatagggttttggggtcagatgtgatcgaagagattgccctagccagaggaaaaacaactgctcagatatcattgagatgggtgtatgaacaaggtgtgagcatagtaacaaaaagctacaacaaggaaagaatgaggcagaaccttgacatcttcgacttctgcttgaccgaggaggaattggagaagatgaatcacctcgtgatctccgcctctgattccggcgaccaccggcacggcgccctccctaatctcgctcgctctctcagcaaaccctatctccttctgctaggcttcgtcgtctccgcgatgatgaagttttcgcagactactgcgaaccagattcgatcagctcctcctacaaagcctcaacgatgccaactcggactctgttcacctataactgactcaggtagtacctctctccttctcgctttttcttcaattccggctttgtttgcttctgattctcatttcggagccctagaatcctctgttttggtttcggagtattcggaatttggacggaacttcatttttgttcggtttcgattttggacgtttagtgagactattagaactcaaaacggggttttgtttgtcgttttggcttgctcaatgagcttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

268

Amino Acids

29.55

Weight (kDa)

7.63

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 39 - 126 1.1e-07 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 416
AccBSI CCGCTC 1 cut(s) 148
AccII CGCG 1 cut(s) 485
AciI CCGC 3 cut(s) 146, 387, 483
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 417
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 1 cut(s) 595
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 1 cut(s) 621
AluBI AGCT 5 cut(s) 76, 158, 294, 530, 801
AluI AGCT 5 cut(s) 76, 158, 294, 530, 801
Alw26I GTCTC 2 cut(s) 484, 740
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 2 cut(s) 22, 102
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 695
AspLEI GCGC 1 cut(s) 419
AsuHPI GGTGA 3 cut(s) 185, 365, 564
AvaI CYCGRG 1 cut(s) 68
BanI GGYRCC 1 cut(s) 416
BanII GRGCYC 1 cut(s) 661
BauI CACGAG 1 cut(s) 377
BbvI GCAGC 1 cut(s) 167
BccI CCATC 1 cut(s) 249
BceAI ACGGC 3 cut(s) 89, 150, 430
BcgI CGANNNNNNTGC 5 cut(s) 35, 49, 83, 194, 228
BcoDI GTCTC 2 cut(s) 484, 740
BfaI CTAG 5 cut(s) 30, 159, 218, 467, 662
BfoI RGCGCY 1 cut(s) 420
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 68
BmiI GGNNCC 2 cut(s) 418, 658
BmsI GCATC 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 348
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 3 cut(s) 254, 457, 600
BseRI GAGGAG 3 cut(s) 86, 365, 522
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 167
Bsh1236I CGCG 1 cut(s) 485
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 24, 104
BshNI GGYRCC 1 cut(s) 416
BsiEI CGRYCG 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 4 cut(s) 101, 399, 409, 626
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 2 cut(s) 484, 740
BsmBI CGTCTC 1 cut(s) 484
BsnI GGCC 2 cut(s) 24, 104
BsoBI CYCGRG 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 3 cut(s) 201, 381, 524
BspACI CCGC 3 cut(s) 146, 387, 483
BspANI GGCC 2 cut(s) 24, 104
BspCNI CTCAG 3 cut(s) 253, 456, 599
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 2 cut(s) 418, 658
BspT107I GGYRCC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 3 cut(s) 201, 381, 524
BssNI GRCGYC 1 cut(s) 417
BssSI CACGAG 1 cut(s) 377
Bst2BI CACGAG 1 cut(s) 377
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 436, 790
BstDEI CTNAG 3 cut(s) 240, 443, 586
BstFNI CGCG 1 cut(s) 485
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 3 cut(s) 204, 384, 527
BstMAI GTCTC 2 cut(s) 484, 740
BstMBI GATC 3 cut(s) 201, 381, 524
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 5 cut(s) 73, 161, 444, 635, 798
BstNSI RCATGY 1 cut(s) 60
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 1 cut(s) 167
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 2 cut(s) 24, 104
BtgZI GCGATG 1 cut(s) 500
Cac8I GCNNGC 2 cut(s) 436, 790
CfoI GCGC 1 cut(s) 419
Cfr10I RCCGGY 1 cut(s) 408
Csp6I GTAC 1 cut(s) 594
CviAII CATG 2 cut(s) 57, 128
CviQI GTAC 1 cut(s) 594
DdeI CTNAG 3 cut(s) 240, 443, 586
DinI GGCGCC 1 cut(s) 418
DpnI GATC 3 cut(s) 203, 383, 526
DpnII GATC 3 cut(s) 201, 381, 524
DraIII CACNNNGTG 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 376
EclXI CGGCCG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 661
Eco32I GATATC 1 cut(s) 246
Eco52I CGGCCG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 68
EcoRV GATATC 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 661
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 484
FaeI CATG 2 cut(s) 60, 131
FaiI YATR 6 cut(s) 58, 65, 129, 264, 281, 578
FatI CATG 2 cut(s) 56, 127
Fnu4HI GCNGC 1 cut(s) 156
FriOI GRGCYC 1 cut(s) 661
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 5 cut(s) 30, 159, 218, 467, 662
GlaI GCGC 1 cut(s) 418
GluI GCNGC 1 cut(s) 156
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 2 cut(s) 24, 104
HapII CCGG 4 cut(s) 101, 399, 409, 626
HhaI GCGC 1 cut(s) 419
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 2 cut(s) 60, 131
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 7 cut(s) 370, 395, 520, 564, 584, 644, 665
HpaII CCGG 4 cut(s) 101, 399, 409, 626
HphI GGTGA 3 cut(s) 185, 365, 564
Hpy166II GTNNAC 2 cut(s) 109, 572
Hpy188III TCNNGA 1 cut(s) 379
Hpy8I GTNNAC 2 cut(s) 109, 572
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 3 cut(s) 94, 470, 614
HpyCH4IV ACGT 1 cut(s) 737
HpyCH4V TGCA 2 cut(s) 12, 60
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 161, 444, 635, 798
HpyF3I CTNAG 3 cut(s) 240, 443, 586
HpySE526I ACGT 1 cut(s) 737
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 2 cut(s) 60, 131
HspAI GCGC 1 cut(s) 417
KasI GGCGCC 1 cut(s) 416
Kzo9I GATC 3 cut(s) 201, 381, 524
LmnI GCTCC 4 cut(s) 73, 152, 535, 656
LpnPI CCDG 7 cut(s) 114, 235, 412, 422, 530, 573, 639
Lsp1109I GCAGC 1 cut(s) 167
LweI GCATC 1 cut(s) 542
MaeI CTAG 5 cut(s) 30, 159, 218, 467, 662
MaeII ACGT 1 cut(s) 737
MaeIII GTNAC 4 cut(s) 47, 130, 173, 283
MalI GATC 3 cut(s) 203, 383, 526
MbiI CCGCTC 1 cut(s) 148
MboI GATC 3 cut(s) 201, 381, 524
MboII GAAGA 5 cut(s) 17, 218, 322, 376, 609
MhlI GDGCHC 1 cut(s) 661
MluCI AATT 3 cut(s) 356, 621, 695
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 2 cut(s) 558, 578
MslI CAYNNNNRTG 1 cut(s) 253
MspI CCGG 4 cut(s) 101, 399, 409, 626
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 5 cut(s) 73, 161, 444, 635, 798
NarI GGCGCC 1 cut(s) 417
NdeII GATC 3 cut(s) 201, 381, 524
NlaIII CATG 2 cut(s) 60, 131
NlaIV GGNNCC 2 cut(s) 418, 658
NmuCI GTSAC 2 cut(s) 130, 173
NspI RCATGY 1 cut(s) 60
PaeR7I CTCGAG 1 cut(s) 68
PfeI GAWTC 5 cut(s) 370, 395, 520, 644, 665
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 2 cut(s) 558, 578
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 2 cut(s) 558, 578
PspN4I GGNNCC 2 cut(s) 418, 658
PspXI VCTCGAGB 1 cut(s) 68
RsaI GTAC 1 cut(s) 595
RsaNI GTAC 1 cut(s) 594
RseI CAYNNNNRTG 1 cut(s) 253
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 3 cut(s) 201, 381, 524
SchI GAGTC 2 cut(s) 558, 578
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 1 cut(s) 542
SfoI GGCGCC 1 cut(s) 418
Sfr274I CTCGAG 1 cut(s) 68
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 3 cut(s) 356, 621, 695
SsiI CCGC 3 cut(s) 146, 387, 483
SspDI GGCGCC 1 cut(s) 416
SspMI CTAG 5 cut(s) 30, 159, 218, 467, 662
TaiI ACGT 1 cut(s) 740
TaqI TCGA 5 cut(s) 69, 204, 333, 523, 727
TaqII GACCGA 1 cut(s) 362
TasI AATT 3 cut(s) 356, 621, 695
TfiI GAWTC 5 cut(s) 370, 395, 520, 644, 665
TseFI GTSAC 2 cut(s) 130, 173
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 2 cut(s) 130, 173
TspDTI ATGAA 5 cut(s) 116, 279, 383, 506, 700
TspGWI ACGGA 1 cut(s) 718
XapI RAATTY 1 cut(s) 695
XceI RCATGY 1 cut(s) 60
XhoI CTCGAG 1 cut(s) 68
XspI CTAG 5 cut(s) 30, 159, 218, 467, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.