Rroxscaffold_2G00096360

ThiF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005521
Physical Location & Seq
Reverse (-)
17591490 .. 17594677
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00096360.1

Sequence Viewer

Length: 216 bp
ATGCTCCAAAAAATGAATCTCAAGCAGTATCTCAATCAGGGCATAGGTCGCATAGGAAGTGTAGCAGCTGAGATGCTAACAAGATGTGGTGTTAATCGCATTCTGTTGTATGATTATGACAAAGTAGAGCTAGATAACTTGAATATGTTATTCTTTCGTCCAGAATATTTCGTAAGGAGCCATTCTTCTATGGACATGATAACTACGATCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThiF

Protein Analysis

71

Amino Acids

8.29

Weight (kDa)

7.89

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 5 - 52 2.4e-11 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 1 cut(s) 142
AluBI AGCT 3 cut(s) 68, 130, 213
AluI AGCT 3 cut(s) 68, 130, 213
ApeKI GCWGC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 77
BfaI CTAG 2 cut(s) 131, 214
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 179
BmsI GCATC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 5
BseMII CTCAG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 99
Bsp143I GATC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 179
BssMI GATC 1 cut(s) 207
BstDEI CTNAG 1 cut(s) 69
BstKTI GATC 1 cut(s) 210
BstMBI GATC 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 77
CviAII CATG 1 cut(s) 196
CviJI RGCY 4 cut(s) 68, 130, 180, 213
CviKI_1 RGCY 4 cut(s) 68, 130, 180, 213
DdeI CTNAG 1 cut(s) 69
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
FaeI CATG 1 cut(s) 199
FaiI YATR 7 cut(s) 44, 53, 111, 117, 146, 191, 197
FatI CATG 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 2 cut(s) 131, 214
GluI GCNGC 1 cut(s) 66
Hin1II CATG 1 cut(s) 199
HinfI GANTC 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 161
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 1 cut(s) 199
Kzo9I GATC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 9, 177
LpnPI CCDG 2 cut(s) 23, 174
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 1 cut(s) 63
MaeI CTAG 2 cut(s) 131, 214
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MboII GAAGA 1 cut(s) 177
MseI TTAA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 68
Mva1269I GAATGC 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 48
NdeII GATC 1 cut(s) 207
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 1 cut(s) 179
PctI GAATGC 1 cut(s) 99
PfeI GAWTC 1 cut(s) 16
PkrI GCNGC 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 68
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 1 cut(s) 207
SetI ASST 4 cut(s) 49, 70, 132, 215
SfaNI GCATC 1 cut(s) 63
SgeI CNNG 7 cut(s) 34, 50, 93, 143, 151, 173, 208
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SspI AATATT 1 cut(s) 167
SspMI CTAG 2 cut(s) 131, 214
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseI GCWGC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 29
XspI CTAG 2 cut(s) 131, 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.