Rmu_sc0001132.1_g000021

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001132.1
OG0002206
Physical Location & Seq
Forward (+)
83438 .. 91286
7849 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3816 bp
atgccaaagaatgtgggtgatttcgactttgcctttgttgatgctgacaaaggtaactattggaattaccacgagaggttgataaagctggtgaagattggtggcattgttatccatgataacacattgtggggaggagcagtagttcagcctgaagaaactattctggagatcaaaagggcgctgaggcaacagatactgaatccaatgggagcgtcaccagatgccaacggagatataggtatgagtagcaagggaaagggcttttccaatggggatattgaggacatggatattgatgacatggattttgaggatgatgaagttgatgattcgactagcattcatgatctgggagaagtgtttctcaaggatttctgtaaggaggcatcagtgtcattcttcaaggagtatggccttctcagtcaccagataaactcatacaatgacttcgttaaaaatggcatacaaagggtctttgattctttcggtgagattgtagttgagcctgggtatgacccatcgaagaagggagatgctgagtggcgatttgcatctgtcaaatttgggaaggtcacccttaaccgaccctgtttttggggaggttctgacaatgataaggagtataatatgttacccagacatgccaggcttcagaacatgacatactcagccaggatgaaagtcaatattacggtccaggtatatactcaaaatcttgtcaaaagtgacaagtttaaaactggaagagagcaatatctggacaagcagactgtgagtactgatagcagggacatcataattggtcgggtacctgtaatggtgaagtctgacttgtgttggatgaaggaagtcgagaaaggtgactgtgagtttgaccagggaggctactttataatcaagggtgcagagaagacgtttatcgctcaagaacagagctgtttgaagagacttctgattacaaataatcagggtctcacagtagcatataggtcagaggtcaagcggcataggctcataattagattatccgggatttcgaaacttgaaaatgttgaaggagtggagaaagtcctgtcagtatactttatgtcaacagaaatccccgtttggatttggtttttcgcccttggggttagttcagacaaagaagttatcgatctcattgattatggcaaagaagatgcaagcatttcaaacatactgtttgcctcaattcgtgatgctgatgagagaaaagatgactttcgtaggggcaagaatgctctgaagtttgttgaattagcgataaagaatacgacgtttccacctggggaaagcattgaggactgcattagcatgtatctgtttcctaatctgagaggcatgaagcagaaggctcggtttctagggtatatggtgaagtgtcttttacagactttcagtggccgcaggaaatgcgataatagggatgactttagaaacaagagattggagttagcaggtgagcttctagaacgagagctgaaggcacatatttcacatgcacggaggcgcatggcaaaggctttgcagagagatctatgtggtgaacgtgttgtccgcccggtggagcactatctagatgcatctatagttaccagtggtgtttccagagcattctctactggagcatggtgtcatcctttcaagaggatggagaggatatcaggtgtggtcgcaaatgttgggcgagcaaatccactgcagacaatgattgatatgaggaagacacgccagcaagttcaatacactggcaaggctggagatgccagatatccgcatccttctcactggggtaaggtttgctttctctcaaccccagatggtgagaactgtggactcgtaaaaaatttggctaccactgctcttgtaagtacaaacatattggagcccatattgcgcaaattgttccgatgtggcatggaggagttggtagatgatacttctacttcactggatgggaagcataaagtttttctgaatggtgactgggttggcgtttgtgtagattcccatggttttgtcacgaaactaaggacattgcgacgcgggaaaatgttgccacacgaggtggaaatcaaaagagatgaacgccaaggagaagtgcgcatattttctgatgctggaaggattctacgtccgctcttggtagtccagaatttgaaaaagattagagcagcaaaaggagaaaaacttaccttcaaagattttcttgacaagggaataattgagctcattggcccggaagaagaagaggattgccgtacagcatgggccattaagtatctgtttatggaagaacagggaaactcagttgtcaagtacacacactgtgagctggacatgtcgtttttgttgggtttaagctgtggtatcattccctttgcaaatcatgatcatgcaaggagggtgctgtaccagtctcagaagcactctcagcaggccattggtttttctactacaaatcctaacctcagagtggatactttgtctcacaacttacactacccccagaggccgctctttcgtacaatgatatctgattcccttggaaaaccaggatacccggtgggacataacggtatcatgccaaagcctgagtacttcaatggccagaatgcaattgttgcagtcaacgtgcacctcggatataaccaagaagattcaatagtaatgaaccgggcatcactagaacgtggtatgttccgatctgagcacataaggagttacaaagctgaagttgaaaacaagctctcattggaaaaaagacgaaaaccagatgagtgtgtaaattttggcaaaatccagagtaaatttggacgtgttgacagccttgatgatgatggattcccctgtgttggtgcaaatctgcagaatggggatattattattgggaggtgcagtgaatctggtactgatcatagcatcaaattgaagcatactgaaaggggaatggtgcagaaggttgtgctttcctctaatgacgatggcaagaattttgctgttgtatcgttaagacaggttcgttctccttctcttggagacaagttctcaagcatgcatgggcaaaagggtgtcctaggttttttggagtctcaagagaatttccctttcacaatacaagggatagttccagatattgtaattaatccacatgcgtttccttcaagacaaactcccggtcaactcctagaggctgctttagggaaggggattgcctgtggtggttcaaagagatacgcgacccctttctctactctctctgttgatgacatcacagaccagcttcatagagctggattttcaagatggggaaatgagagagtatacaatggtcgaactggtgaaatggttcgccaactgatattcatgggcccaacattctatcaacgtctgattcacatgtctgaagacaaagtaaagtttaggaacactgggccagttcaccctctcacccggcagccagtggcagacaggaagcgatttggtgggatcaaatttggtgagatggagcgcgactgtcttatagcccatggagcatcagcaaacttgcatgaacgcctcttcactctcagtgactcctccctgatgaacgtttgccagaaatgccagaatgcagccaatgtgatagagcggaaagtgactggtggccgcaaaatcaggggtccctattgccgggtctgcgactctgctgatgacattgtgagggtgaacgtgccatatggggccaagctattgtgccaggagctattcagcatgggcatcagtctcaagtttgaaaccaggctctgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1271

Amino Acids

143.58

Weight (kDa)

8.7

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_3 PF01596 4 - 60 4.5e-18 O-methyltransferase
RNA_pol_Rpb2_1 PF04563 139 - 523 7.2e-35 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 353 - 492 2e-11 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 571 - 630 6.7e-15 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 665 - 726 4.4e-22 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 741 - 788 3.7e-06 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 797 - 1168 8.5e-111 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 1170 - 1267 3e-26 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
AarI CACCTGC 1 cut(s) 1472
Acc16I TGCGCA 2 cut(s) 1922, 2129
Acc36I ACCTGC 1 cut(s) 1472
Acc65I GGTACC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 811
AccBSI CCGCTC 3 cut(s) 2164, 2542, 3655
AccI GTMKAC 2 cut(s) 1083, 3348
AccII CGCG 3 cut(s) 2070, 3263, 3537
AciI CCGC 9 cut(s) 1006, 1429, 1582, 1799, 2070, 2162, 2540, 3655, 3673
AclI AACGTT 1 cut(s) 3615
AclWI GGATC 1 cut(s) 3521
AcoI YGGCCR 3 cut(s) 1426, 2632, 3670
AcsI RAATTY 8 cut(s) 563, 1870, 2179, 2812, 2834, 3016, 3124, 3518
AcuI CTGAAG 6 cut(s) 174, 638, 1289, 1526, 2778, 3450
AdeI CACNNNGTG 3 cut(s) 129, 2092, 2354
AfaI GTAC 9 cut(s) 781, 813, 1897, 2287, 2345, 2438, 2551, 2624, 2935
AfiI CCNNNNNNNGG 6 cut(s) 597, 1588, 2500, 2879, 3059, 3696
AflIII ACRYGT 4 cut(s) 1573, 2364, 2842, 3423
AjiI CACGTC 1 cut(s) 2843
AjnI CCWGG 9 cut(s) 508, 647, 674, 699, 879, 1309, 2578, 3762, 3803
AloI GAACNNNNNNTCC 4 cut(s) 129, 161, 1563, 1595
Alw21I GWGCWC 4 cut(s) 1596, 2256, 2664, 2739
Alw26I GTCTC 7 cut(s) 943, 980, 2448, 2517, 3057, 3120, 3794
Alw44I GTGCAC 1 cut(s) 2660
AlwI GGATC 1 cut(s) 3521
AlwNI CAGNNNCTG 2 cut(s) 199, 3810
ApaI GGGCCC 1 cut(s) 3398
ApaLI GTGCAC 1 cut(s) 2660
ApeKI GCWGC 4 cut(s) 2198, 3218, 3481, 3638
ApoI RAATTY 8 cut(s) 563, 1870, 2179, 2812, 2834, 3016, 3124, 3518
AseI ATTAAT 1 cut(s) 3168
Asp700I GAANNNNTTC 3 cut(s) 162, 363, 3372
Asp718I GGTACC 1 cut(s) 811
AspA2I CCTAGG 1 cut(s) 3100
AspLEI GCGC 5 cut(s) 184, 1536, 1923, 2130, 3537
AspS9I GGNCC 8 cut(s) 697, 2261, 2295, 3394, 3395, 3458, 3686, 3747
AsuC2I CCSGG 8 cut(s) 1033, 1586, 2264, 2588, 2702, 3201, 3478, 3698
AsuII TTCGAA 1 cut(s) 1040
AvaII GGWCC 2 cut(s) 697, 3686
AvrII CCTAGG 1 cut(s) 3100
BaeGI GKGCMC 2 cut(s) 2664, 3398
BalI TGGCCA 1 cut(s) 2634
BanI GGYRCC 1 cut(s) 811
BanII GRGCYC 3 cut(s) 1914, 2256, 3398
BauI CACGAG 2 cut(s) 71, 2087
BbsI GAAGAC 3 cut(s) 920, 1754, 3438
Bbv12I GWGCWC 4 cut(s) 1596, 2256, 2664, 2739
BbvCI CCTCAGC 1 cut(s) 185
BbvI GCAGC 4 cut(s) 2210, 3205, 3493, 3650
BccI CCATC 8 cut(s) 529, 1669, 1838, 1973, 2858, 3002, 3324, 3523
BceAI ACGGC 1 cut(s) 2268
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BciT130I CCWGG 9 cut(s) 510, 649, 676, 701, 881, 1311, 2580, 3764, 3805
BciVI GTATCC 2 cut(s) 2497, 2576
BclI TGATCA 2 cut(s) 2416, 2938
BcnI CCSGG 8 cut(s) 1033, 1586, 2264, 2588, 2702, 3201, 3478, 3698
BcoDI GTCTC 7 cut(s) 943, 980, 2448, 2517, 3057, 3120, 3794
BfaI CTAG 7 cut(s) 339, 1388, 1493, 1601, 2711, 3101, 3212
BfmI CTRYAG 3 cut(s) 1611, 1724, 2891
BfoI RGCGCY 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 1472
BfuI GTATCC 2 cut(s) 2497, 2576
BglII AGATCT 1 cut(s) 1558
BisI GCNGC 8 cut(s) 1007, 1429, 2199, 2540, 3219, 3482, 3639, 3673
BlnI CCTAGG 1 cut(s) 3100
BlsI GCNGC 8 cut(s) 1008, 1430, 2200, 2541, 3220, 3483, 3640, 3674
BmcAI AGTACT 2 cut(s) 781, 2624
Bme18I GGWCC 2 cut(s) 697, 3686
BmgBI CACGTC 1 cut(s) 2843
BmgT120I GGNCC 8 cut(s) 697, 2261, 2295, 3394, 3395, 3458, 3686, 3747
BmiI GGNNCC 6 cut(s) 813, 1911, 3396, 3687, 3688, 3748
BmrI ACTGGG 3 cut(s) 1822, 2020, 3465
BmuI ACTGGG 3 cut(s) 1822, 2020, 3465
BpiI GAAGAC 3 cut(s) 920, 1754, 3438
BpmI CTGGAG 3 cut(s) 188, 1668, 1803
Bpu10I CCTNAGC 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 1040
BpuEI CTTGAG 5 cut(s) 353, 912, 3058, 3102, 3776
BpuMI CCSGG 8 cut(s) 1033, 1586, 2264, 2588, 2702, 3201, 3478, 3698
Bsa29I ATCGAT 1 cut(s) 1158
BsaBI GATNNNNATC 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 980
BsaXI ACNNNNNCTCC 6 cut(s) 126, 129, 156, 159, 2908, 2938
Bsc4I CCNNNNNNNGG 6 cut(s) 597, 1588, 2500, 2879, 3059, 3696
Bse3DI GCAATG 1 cut(s) 2060
Bse8I GATNNNNATC 1 cut(s) 553
BseBI CCWGG 9 cut(s) 510, 649, 676, 701, 881, 1311, 2580, 3764, 3805
BseCI ATCGAT 1 cut(s) 1158
BseGI GGATG 8 cut(s) 322, 684, 849, 1456, 1660, 1680, 1801, 1984
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 6 cut(s) 597, 1588, 2500, 2879, 3059, 3696
BseMI GCAATG 1 cut(s) 2060
BseRI GAGGAG 3 cut(s) 150, 1961, 3592
BseSI GKGCMC 2 cut(s) 2664, 3398
BseXI GCAGC 4 cut(s) 2210, 3205, 3493, 3650
BsgI GTGCAG 3 cut(s) 927, 2941, 2999
Bsh1236I CGCG 3 cut(s) 2070, 3263, 3537
BshNI GGYRCC 1 cut(s) 811
BshVI ATCGAT 1 cut(s) 1158
BsiHKAI GWGCWC 4 cut(s) 1596, 2256, 2664, 2739
BsiSI CCGG 8 cut(s) 1032, 1586, 2264, 2588, 2701, 3201, 3478, 3697
BslFI GGGAC 3 cut(s) 806, 2607, 3672
BslI CCNNNNNNNGG 6 cut(s) 597, 1588, 2500, 2879, 3059, 3696
BsmAI GTCTC 7 cut(s) 943, 980, 2448, 2517, 3057, 3120, 3794
BsmFI GGGAC 3 cut(s) 806, 2607, 3672
BsmI GAATGC 5 cut(s) 342, 1267, 1637, 2644, 3640
Bso31I GGTCTC 1 cut(s) 980
Bsp119I TTCGAA 1 cut(s) 1040
Bsp120I GGGCCC 1 cut(s) 3394
Bsp1286I GDGCHC 6 cut(s) 1596, 1914, 2256, 2664, 2739, 3398
Bsp143I GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
Bsp19I CCATGG 2 cut(s) 2035, 3553
BspACI CCGC 9 cut(s) 1006, 1429, 1582, 1799, 2070, 2162, 2540, 3655, 3673
BspDI ATCGAT 1 cut(s) 1158
BspFNI CGCG 3 cut(s) 2070, 3263, 3537
BspHI TCATGA 2 cut(s) 346, 2413
BspLI GGNNCC 6 cut(s) 813, 1911, 3396, 3687, 3688, 3748
BspMAI CTGCAG 2 cut(s) 1728, 2895
BspMI ACCTGC 1 cut(s) 1472
BspPI GGATC 1 cut(s) 3521
BspT104I TTCGAA 1 cut(s) 1040
BspT107I GGYRCC 1 cut(s) 811
BspTNI GGTCTC 1 cut(s) 980
BsrBI CCGCTC 3 cut(s) 2164, 2542, 3655
BsrDI GCAATG 1 cut(s) 2060
BssMI GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
BssNAI GTATAC 2 cut(s) 1084, 3349
BssSI CACGAG 2 cut(s) 71, 2087
BssT1I CCWWGG 6 cut(s) 1129, 2035, 2116, 2569, 3100, 3553
Bst1107I GTATAC 2 cut(s) 1084, 3349
Bst2BI CACGAG 2 cut(s) 71, 2087
Bst2UI CCWGG 9 cut(s) 510, 649, 676, 701, 881, 1311, 2580, 3764, 3805
Bst4CI ACNGT 9 cut(s) 697, 775, 869, 982, 1206, 1856, 2354, 2603, 3542
Bst6I CTCTTC 4 cut(s) 742, 941, 2268, 3590
BstAPI GCANNNNNTGC 2 cut(s) 1437, 2648
BstBI TTCGAA 1 cut(s) 1040
BstC8I GCNNGC 5 cut(s) 1189, 1713, 1757, 2463, 3080
BstDSI CCRYGG 2 cut(s) 2035, 3553
BstEII GGTNACC 1 cut(s) 574
BstF5I GGATG 8 cut(s) 322, 684, 849, 1456, 1660, 1680, 1801, 1984
BstFNI CGCG 3 cut(s) 2070, 3263, 3537
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 5 cut(s) 184, 1536, 1923, 2130, 3537
BstKTI GATC 8 cut(s) 174, 352, 1162, 1561, 2419, 2732, 2941, 3516
BstMAI GTCTC 7 cut(s) 943, 980, 2448, 2517, 3057, 3120, 3794
BstMBI GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
BstNI CCWGG 9 cut(s) 510, 649, 676, 701, 881, 1311, 2580, 3764, 3805
BstNSI RCATGY 7 cut(s) 647, 1342, 1526, 2368, 3082, 3179, 3427
BstPI GGTNACC 1 cut(s) 574
BstSFI CTRYAG 3 cut(s) 1611, 1724, 2891
BstSLI GKGCMC 2 cut(s) 2664, 3398
BstUI CGCG 3 cut(s) 2070, 3263, 3537
BstV1I GCAGC 4 cut(s) 2210, 3205, 3493, 3650
BstV2I GAAGAC 3 cut(s) 920, 1754, 3438
BstX2I RGATCY 1 cut(s) 1558
BstYI RGATCY 1 cut(s) 1558
BstZ17I GTATAC 2 cut(s) 1084, 3349
Bsu15I ATCGAT 1 cut(s) 1158
BsuI GTATCC 2 cut(s) 2497, 2576
BsuTUI ATCGAT 1 cut(s) 1158
BtgI CCRYGG 2 cut(s) 2035, 3553
BtrI CACGTC 1 cut(s) 2843
BtsCI GGATG 8 cut(s) 322, 684, 849, 1456, 1660, 1680, 1801, 1984
BtsI GCAGTG 3 cut(s) 1721, 1881, 2929
BveI ACCTGC 1 cut(s) 1472
Cac8I GCNNGC 5 cut(s) 1189, 1713, 1757, 2463, 3080
CaiI CAGNNNCTG 2 cut(s) 199, 3810
CciI TCATGA 2 cut(s) 346, 2413
CfoI GCGC 5 cut(s) 184, 1536, 1923, 2130, 3537
Cfr13I GGNCC 8 cut(s) 697, 2261, 2295, 3394, 3395, 3458, 3686, 3747
ClaI ATCGAT 1 cut(s) 1158
CseI GACGC 2 cut(s) 204, 2076
Csp6I GTAC 9 cut(s) 780, 812, 1896, 2286, 2344, 2437, 2550, 2623, 2934
CspCI CAANNNNNGTGG 3 cut(s) 29, 1870, 1905
CviQI GTAC 9 cut(s) 780, 812, 1896, 2286, 2344, 2437, 2550, 2623, 2934
DpnI GATC 8 cut(s) 173, 351, 1161, 1560, 2418, 2731, 2940, 3515
DpnII GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
DraI TTTAAA 1 cut(s) 739
DraIII CACNNNGTG 3 cut(s) 129, 2092, 2354
EaeI YGGCCR 3 cut(s) 1426, 2632, 3670
Eam1104I CTCTTC 4 cut(s) 742, 941, 2268, 3590
EarI CTCTTC 4 cut(s) 742, 941, 2268, 3590
EciI GGCGGA 1 cut(s) 1571
Ecl136II GAGCTC 1 cut(s) 2254
Eco130I CCWWGG 6 cut(s) 1129, 2035, 2116, 2569, 3100, 3553
Eco24I GRGCYC 3 cut(s) 1914, 2256, 3398
Eco31I GGTCTC 1 cut(s) 980
Eco32I GATATC 3 cut(s) 1686, 1796, 2559
Eco47I GGWCC 2 cut(s) 697, 3686
Eco53kI GAGCTC 1 cut(s) 2254
Eco57I CTGAAG 6 cut(s) 174, 638, 1289, 1526, 2778, 3450
Eco91I GGTNACC 1 cut(s) 574
EcoICRI GAGCTC 1 cut(s) 2254
EcoO109I RGGNCCY 1 cut(s) 3686
EcoO65I GGTNACC 1 cut(s) 574
EcoRII CCWGG 9 cut(s) 508, 647, 674, 699, 879, 1309, 2578, 3762, 3803
EcoRV GATATC 3 cut(s) 1686, 1796, 2559
EcoT14I CCWWGG 6 cut(s) 1129, 2035, 2116, 2569, 3100, 3553
EcoT22I ATGCAT 2 cut(s) 1609, 3084
EcoT38I GRGCYC 3 cut(s) 1914, 2256, 3398
ErhI CCWWGG 6 cut(s) 1129, 2035, 2116, 2569, 3100, 3553
FalI AAGNNNNNCTT 6 cut(s) 818, 850, 1811, 1843, 2217, 2249
FaqI GGGAC 3 cut(s) 806, 2607, 3672
FauI CCCGC 1 cut(s) 2063
FauNDI CATATG 1 cut(s) 3742
FbaI TGATCA 2 cut(s) 2416, 2938
FblI GTMKAC 2 cut(s) 1083, 3348
Fnu4HI GCNGC 8 cut(s) 1007, 1429, 2199, 2540, 3219, 3482, 3639, 3673
FokI GGATG 8 cut(s) 329, 691, 856, 1463, 1647, 1687, 1788, 1991
FriOI GRGCYC 3 cut(s) 1914, 2256, 3398
Fsp4HI GCNGC 8 cut(s) 1007, 1429, 2199, 2540, 3219, 3482, 3639, 3673
FspAI RTGCGCAY 1 cut(s) 2129
FspBI CTAG 7 cut(s) 339, 1388, 1493, 1601, 2711, 3101, 3212
FspI TGCGCA 2 cut(s) 1922, 2129
GlaI GCGC 5 cut(s) 183, 1535, 1922, 2129, 3536
GluI GCNGC 8 cut(s) 1007, 1429, 2199, 2540, 3219, 3482, 3639, 3673
GsuI CTGGAG 3 cut(s) 188, 1668, 1803
HaeII RGCGCY 1 cut(s) 185
HapII CCGG 8 cut(s) 1032, 1586, 2264, 2588, 2701, 3201, 3478, 3697
HgaI GACGC 2 cut(s) 204, 2076
HhaI GCGC 5 cut(s) 184, 1536, 1923, 2130, 3537
Hin6I GCGC 5 cut(s) 182, 1534, 1921, 2128, 3535
HinP1I GCGC 5 cut(s) 182, 1534, 1921, 2128, 3535
HincII GTYRAC 4 cut(s) 1095, 2656, 2848, 3206
HindII GTYRAC 4 cut(s) 1095, 2656, 2848, 3206
HpaII CCGG 8 cut(s) 1032, 1586, 2264, 2588, 2701, 3201, 3478, 3697
Hpy99I CGWCG 2 cut(s) 1303, 2070
HpyCH4III ACNGT 9 cut(s) 697, 775, 869, 982, 1206, 1856, 2354, 2603, 3542
HspAI GCGC 5 cut(s) 182, 1534, 1921, 2128, 3535
KflI GGGWCCC 1 cut(s) 3686
KpnI GGTACC 1 cut(s) 815
Ksp22I TGATCA 2 cut(s) 2416, 2938
Kzo9I GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
LmnI GCTCC 8 cut(s) 137, 212, 1591, 1649, 1909, 3532, 3557, 3766
Lsp1109I GCAGC 4 cut(s) 2210, 3205, 3493, 3650
MaeI CTAG 7 cut(s) 339, 1388, 1493, 1601, 2711, 3101, 3212
MalI GATC 8 cut(s) 173, 351, 1161, 1560, 2418, 2731, 2940, 3515
MbiI CCGCTC 3 cut(s) 2164, 2542, 3655
MboI GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
MfeI CAATTG 1 cut(s) 2643
MflI RGATCY 1 cut(s) 1558
MhlI GDGCHC 6 cut(s) 1596, 1914, 2256, 2664, 2739, 3398
MlsI TGGCCA 1 cut(s) 2634
MluNI TGGCCA 1 cut(s) 2634
MlyI GAGTC 4 cut(s) 1854, 3122, 3593, 3701
MmeI TCCRAC 1 cut(s) 821
Mox20I TGGCCA 1 cut(s) 2634
Mph1103I ATGCAT 2 cut(s) 1609, 3084
MroXI GAANNNNTTC 3 cut(s) 162, 363, 3372
MscI TGGCCA 1 cut(s) 2634
MseI TTAA 7 cut(s) 456, 582, 738, 2301, 2384, 3035, 3168
MslI CAYNNNNRTG 2 cut(s) 1337, 2418
Msp20I TGGCCA 1 cut(s) 2634
MspI CCGG 8 cut(s) 1032, 1586, 2264, 2588, 2701, 3201, 3478, 3697
MunI CAATTG 1 cut(s) 2643
Mva1269I GAATGC 5 cut(s) 342, 1267, 1637, 2644, 3640
MvaI CCWGG 9 cut(s) 510, 649, 676, 701, 881, 1311, 2580, 3764, 3805
MvnI CGCG 3 cut(s) 2070, 3263, 3537
NciI CCSGG 8 cut(s) 1033, 1586, 2264, 2588, 2702, 3201, 3478, 3698
NcoI CCATGG 2 cut(s) 2035, 3553
NdeI CATATG 1 cut(s) 3742
NdeII GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
NlaIV GGNNCC 6 cut(s) 813, 1911, 3396, 3687, 3688, 3748
NmuCI GTSAC 9 cut(s) 216, 425, 574, 728, 863, 2006, 2044, 3596, 3661
NsbI TGCGCA 2 cut(s) 1922, 2129
NsiI ATGCAT 2 cut(s) 1609, 3084
NspI RCATGY 7 cut(s) 647, 1342, 1526, 2368, 3082, 3179, 3427
NspV TTCGAA 1 cut(s) 1040
PaeI GCATGC 1 cut(s) 3082
PagI TCATGA 2 cut(s) 346, 2413
PaqCI CACCTGC 1 cut(s) 1472
PciI ACATGT 2 cut(s) 2364, 3423
PctI GAATGC 5 cut(s) 342, 1267, 1637, 2644, 3640
PdmI GAANNNNTTC 3 cut(s) 162, 363, 3372
PfoI TCCNGGA 1 cut(s) 1031
PkrI GCNGC 8 cut(s) 1008, 1430, 2200, 2541, 3220, 3483, 3640, 3674
PleI GAGTC 4 cut(s) 1854, 3121, 3593, 3701
PpsI GAGTC 4 cut(s) 1854, 3121, 3593, 3701
PpuMI RGGWCCY 1 cut(s) 3686
PscI ACATGT 2 cut(s) 2364, 3423
PshBI ATTAAT 1 cut(s) 3168
PsiI TTATAA 1 cut(s) 896
Psp124BI GAGCTC 1 cut(s) 2256
Psp1406I AACGTT 1 cut(s) 3615
Psp5II RGGWCCY 1 cut(s) 3686
Psp6I CCWGG 9 cut(s) 508, 647, 674, 699, 879, 1309, 2578, 3762, 3803
PspEI GGTNACC 1 cut(s) 574
PspGI CCWGG 9 cut(s) 508, 647, 674, 699, 879, 1309, 2578, 3762, 3803
PspN4I GGNNCC 6 cut(s) 813, 1911, 3396, 3687, 3688, 3748
PspOMI GGGCCC 1 cut(s) 3394
PspPI GGNCC 8 cut(s) 697, 2261, 2295, 3394, 3395, 3458, 3686, 3747
PspPPI RGGWCCY 1 cut(s) 3686
PsrI GAACNNNNNNTAC 2 cut(s) 650, 682
PstI CTGCAG 2 cut(s) 1728, 2895
PstNI CAGNNNCTG 2 cut(s) 199, 3810
PsuI RGATCY 1 cut(s) 1558
RsaI GTAC 9 cut(s) 781, 813, 1897, 2287, 2345, 2438, 2551, 2624, 2935
RsaNI GTAC 9 cut(s) 780, 812, 1896, 2286, 2344, 2437, 2550, 2623, 2934
RseI CAYNNNNRTG 2 cut(s) 1337, 2418
SacI GAGCTC 1 cut(s) 2256
SaqAI TTAA 7 cut(s) 456, 582, 738, 2301, 2384, 3035, 3168
SatI GCNGC 8 cut(s) 1007, 1429, 2199, 2540, 3219, 3482, 3639, 3673
Sau3AI GATC 8 cut(s) 171, 349, 1159, 1558, 2416, 2729, 2938, 3513
Sau96I GGNCC 8 cut(s) 697, 2261, 2295, 3394, 3395, 3458, 3686, 3747
ScaI AGTACT 2 cut(s) 781, 2624
SchI GAGTC 4 cut(s) 1854, 3122, 3593, 3701
SduI GDGCHC 6 cut(s) 1596, 1914, 2256, 2664, 2739, 3398
SfcI CTRYAG 3 cut(s) 1611, 1724, 2891
SfuI TTCGAA 1 cut(s) 1040
SinI GGWCC 2 cut(s) 697, 3686
SmiMI CAYNNNNRTG 2 cut(s) 1337, 2418
SmlI CTYRAG 5 cut(s) 368, 927, 3073, 3117, 3791
SmoI CTYRAG 5 cut(s) 368, 927, 3073, 3117, 3791
SphI GCATGC 1 cut(s) 3082
SsiI CCGC 9 cut(s) 1006, 1429, 1582, 1799, 2070, 2162, 2540, 3655, 3673
SspI AATATT 1 cut(s) 691
SspMI CTAG 7 cut(s) 339, 1388, 1493, 1601, 2711, 3101, 3212
SstI GAGCTC 1 cut(s) 2256
StyI CCWWGG 6 cut(s) 1129, 2035, 2116, 2569, 3100, 3553
TaaI ACNGT 9 cut(s) 697, 775, 869, 982, 1206, 1856, 2354, 2603, 3542
TaqI TCGA 7 cut(s) 24, 335, 524, 855, 1040, 1158, 3358
TatI WGTACW 4 cut(s) 779, 1895, 2343, 2622
TauI GCSGC 4 cut(s) 1009, 1431, 2542, 3675
Tru1I TTAA 7 cut(s) 456, 582, 738, 2301, 2384, 3035, 3168
Tru9I TTAA 7 cut(s) 456, 582, 738, 2301, 2384, 3035, 3168
TseFI GTSAC 9 cut(s) 216, 425, 574, 728, 863, 2006, 2044, 3596, 3661
TseI GCWGC 4 cut(s) 2198, 3218, 3481, 3638
Tsp45I GTSAC 9 cut(s) 216, 425, 574, 728, 863, 2006, 2044, 3596, 3661
TspGWI ACGGA 2 cut(s) 246, 1543
VneI GTGCAC 1 cut(s) 2660
VpaK11BI GGWCC 2 cut(s) 697, 3686
VspI ATTAAT 1 cut(s) 3168
XapI RAATTY 8 cut(s) 563, 1870, 2179, 2812, 2834, 3016, 3124, 3518
XbaI TCTAGA 2 cut(s) 1492, 1600
XceI RCATGY 7 cut(s) 647, 1342, 1526, 2368, 3082, 3179, 3427
XcmI CCANNNNNNNNNTGG 1 cut(s) 2834
XmaJI CCTAGG 1 cut(s) 3100
XmiI GTMKAC 2 cut(s) 1083, 3348
XmnI GAANNNNTTC 3 cut(s) 162, 363, 3372
XspI CTAG 7 cut(s) 339, 1388, 1493, 1601, 2711, 3101, 3212
ZrmI AGTACT 2 cut(s) 781, 2624
Zsp2I ATGCAT 2 cut(s) 1609, 3084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.