Rmu_sc0001304.1_g000001

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001304.1
OG0004242
Physical Location & Seq
Reverse (-)
2464 .. 3921
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1458 bp
atgcttgacattgatggttgtaaaaaattggctgcacttccgaggatggtggaacttagtacattgtacctgatcgattcaaatgctgaattacttggatccaagatggaatttagctcgctgacctctttgcacctaactgatattccagatgtgaagcatctacctgaaggctttatccaacagtctgcaaaacttgaaaaattgagtcttacaagctttcctgaccttgaatatttggcagatgaccgagttggactagcacaccttgtatcccttcaatgcttgacaatatcccattgtcctgagtttgttgctttgcctgatgagctcccacctgtacttaaatatttggatttgaaacattgtcctagcctgatgaagctgcctggtaaacttcacaagcttgagtgtcttgcagagctgcagattgaatggtgttcaaagattgaagcatttccagatgtggggttgcctgacaaactgaagcgtcttgtaatccgcgaatgtggtgctctgatgacccttcctcaggagcttctctgcaataacaattttctcgagtatttagaagttcataaatgtccttctcttgtgtcagttcttgaagaaggaaacttgccatcaactctaacacatatgaaattttactattgcaaaattctgggatcactgcctgagggtttgatgcacaaagacaatatgactctaaagtacttggagatagacaactgttcctctctcaagtcctttccaaatggtgagttaccaacaagccttgagcgtcttgagattagtgaatgctctaatttccagacattgccattgagccttctcaacctcacgaatcttgagaccttagaagtaaagagctgcccccttcttcaggattttccagagggtggattgccacccaacataaagtttgacacaatttcagaatgtaagaatctcaagtctctacctaaattctttcataaactcaaatgtcttcagaagttggagatttctggctgtcctagtctcctgtccttgccaaagcagggtctgcccagcagcttaaggctacttacagttactgattgtgaaaagcttaatcccatacatcagtggagacttcacaaactaccctctctagatgttttcaccattggtggatttcctggcctgaagtccttttcaaaagattatcctcttcctcacactttaacctctttaagcattcaaagattcccagatcttgaatccatatcggaggtgcttgagaatctcacctcacttgaaaacctgagtatcagggagtgtgataagctccaatctttgctagagactgggctaccagcgacaatttctaccttgacaattcggaattgtttacttctagaacctcgatgtgaaaaggacaaaggggaagattggtccaagatcaaaaacatcccctttgtgagttcacgctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

485

Amino Acids

54.46

Weight (kDa)

5.63

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_EndoS PF23952 236 - 298 3.5e-06 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 902
AccII CGCG 1 cut(s) 504
AciI CCGC 1 cut(s) 502
AclWI GGATC 3 cut(s) 93, 106, 676
AcsI RAATTY 4 cut(s) 110, 644, 660, 968
AcuI CTGAAG 5 cut(s) 189, 506, 869, 977, 1190
AfaI GTAC 4 cut(s) 61, 68, 342, 716
AfiI CCNNNNNNNGG 5 cut(s) 467, 532, 886, 902, 1043
AflII CTTAAG 1 cut(s) 1060
AjnI CCWGG 2 cut(s) 388, 1162
Alw21I GWGCWC 2 cut(s) 333, 517
Alw26I GTCTC 5 cut(s) 848, 963, 1028, 1108, 1322
AlwI GGATC 3 cut(s) 93, 106, 676
AlwNI CAGNNNCTG 2 cut(s) 1048, 1079
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 1 cut(s) 1165
ApeKI GCWGC 5 cut(s) 32, 385, 424, 873, 1056
ApoI RAATTY 4 cut(s) 110, 644, 660, 968
Asp700I GAANNNNTTC 1 cut(s) 456
AspS9I GGNCC 1 cut(s) 1419
AsuHPI GGTGA 3 cut(s) 773, 1138, 1264
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 1 cut(s) 1419
AxyI CCTNAGG 2 cut(s) 531, 678
BaeI ACNNNNGTAYC 2 cut(s) 1276, 1309
BamHI GGATCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 983
Bbv12I GWGCWC 2 cut(s) 333, 517
BbvI GCAGC 5 cut(s) 19, 372, 411, 860, 1068
BccI CCATC 4 cut(s) 8, 40, 100, 631
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 494, 528
BciT130I CCWGG 2 cut(s) 390, 1164
BciVI GTATCC 1 cut(s) 283
BcoDI GTCTC 5 cut(s) 848, 963, 1028, 1108, 1322
BfaI CTAG 6 cut(s) 260, 372, 1020, 1136, 1325, 1382
BfmI CTRYAG 1 cut(s) 425
BfrI CTTAAG 1 cut(s) 1060
BfuI GTATCC 1 cut(s) 283
BglII AGATCT 1 cut(s) 1237
BisI GCNGC 5 cut(s) 33, 386, 425, 874, 1057
BlsI GCNGC 5 cut(s) 34, 387, 426, 875, 1058
BmcAI AGTACT 1 cut(s) 716
Bme1390I CCNGG 2 cut(s) 390, 1164
Bme18I GGWCC 1 cut(s) 1419
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 1419
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 2 cut(s) 390, 1164
BmrI ACTGGG 1 cut(s) 1341
BmsI GCATC 2 cut(s) 169, 678
BmuI ACTGGG 1 cut(s) 1341
BpiI GAAGAC 1 cut(s) 983
BpuEI CTTGAG 7 cut(s) 428, 728, 800, 809, 872, 938, 1283
Bsa29I ATCGAT 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 1 cut(s) 41
Bsc4I CCNNNNNNNGG 5 cut(s) 467, 532, 886, 902, 1043
Bse1I ACTGG 1 cut(s) 1336
Bse21I CCTNAGG 2 cut(s) 531, 678
Bse3DI GCAATG 1 cut(s) 818
BseBI CCWGG 2 cut(s) 390, 1164
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 2 cut(s) 51, 1434
BseLI CCNNNNNNNGG 5 cut(s) 467, 532, 886, 902, 1043
BseMI GCAATG 1 cut(s) 818
BseMII CTCAG 4 cut(s) 297, 545, 669, 1280
BseNI ACTGG 1 cut(s) 1336
BseXI GCAGC 5 cut(s) 19, 372, 411, 860, 1068
BseYI CCCAGC 1 cut(s) 1052
BsgI GTGCAG 1 cut(s) 18
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 1 cut(s) 1167
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 2 cut(s) 333, 517
BsiHKCI CYCGRG 1 cut(s) 560
BslI CCNNNNNNNGG 5 cut(s) 467, 532, 886, 902, 1043
BsmAI GTCTC 5 cut(s) 848, 963, 1028, 1108, 1322
BsmI GAATGC 2 cut(s) 806, 1221
BsnI GGCC 1 cut(s) 1167
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 333, 517
Bsp143I GATC 5 cut(s) 72, 98, 668, 1237, 1425
BspACI CCGC 1 cut(s) 502
BspANI GGCC 1 cut(s) 1167
BspCNI CTCAG 4 cut(s) 298, 544, 670, 1281
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 1 cut(s) 504
BspLI GGNNCC 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 429
BspPI GGATC 3 cut(s) 93, 106, 676
BspTI CTTAAG 1 cut(s) 1060
BspTNI GGTCTC 1 cut(s) 848
BsrDI GCAATG 1 cut(s) 818
BsrI ACTGG 1 cut(s) 1336
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 5 cut(s) 72, 98, 668, 1237, 1425
Bst2UI CCWGG 2 cut(s) 390, 1164
Bst4CI ACNGT 3 cut(s) 186, 734, 1075
Bst6I CTCTTC 1 cut(s) 1200
BstAFI CTTAAG 1 cut(s) 1060
BstAPI GCANNNNNTGC 1 cut(s) 1048
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 6 cut(s) 56, 306, 531, 678, 859, 1289
BstENI CCTNNNNNAGG 2 cut(s) 530, 884
BstF5I GGATG 2 cut(s) 51, 1434
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 5 cut(s) 75, 101, 671, 1240, 1428
BstMAI GTCTC 5 cut(s) 848, 963, 1028, 1108, 1322
BstMBI GATC 5 cut(s) 72, 98, 668, 1237, 1425
BstMWI GCNNNNNNNGC 2 cut(s) 328, 1048
BstNI CCWGG 2 cut(s) 390, 1164
BstSCI CCNGG 2 cut(s) 388, 1162
BstSFI CTRYAG 1 cut(s) 425
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 5 cut(s) 19, 372, 411, 860, 1068
BstV2I GAAGAC 1 cut(s) 983
BstX2I RGATCY 2 cut(s) 98, 1237
BstYI RGATCY 2 cut(s) 98, 1237
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 2 cut(s) 531, 678
BsuI GTATCC 1 cut(s) 283
BsuRI GGCC 1 cut(s) 1167
BsuTUI ATCGAT 1 cut(s) 75
BtsCI GGATG 2 cut(s) 51, 1434
BtsI GCAGTG 1 cut(s) 671
BtsIMutI CAGTG 2 cut(s) 671, 1115
Cac8I GCNNGC 1 cut(s) 119
CaiI CAGNNNCTG 2 cut(s) 1048, 1079
Cfr13I GGNCC 1 cut(s) 1419
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 2 cut(s) 479, 773
Csp6I GTAC 4 cut(s) 60, 67, 341, 715
CviQI GTAC 4 cut(s) 60, 67, 341, 715
DdeI CTNAG 6 cut(s) 56, 306, 531, 678, 859, 1289
DpnI GATC 5 cut(s) 74, 100, 670, 1239, 1427
DpnII GATC 5 cut(s) 72, 98, 668, 1237, 1425
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
Ecl136II GAGCTC 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 848
Eco47I GGWCC 1 cut(s) 1419
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 5 cut(s) 189, 506, 869, 977, 1190
Eco81I CCTNAGG 2 cut(s) 531, 678
Eco88I CYCGRG 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 2 cut(s) 530, 884
EcoRII CCWGG 2 cut(s) 388, 1162
EcoT38I GRGCYC 1 cut(s) 333
FaiI YATR 8 cut(s) 579, 639, 641, 704, 920, 978, 1103, 1250
FauNDI CATATG 1 cut(s) 639
Fnu4HI GCNGC 5 cut(s) 33, 386, 425, 874, 1057
FokI GGATG 2 cut(s) 58, 1421
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 5 cut(s) 33, 386, 425, 874, 1057
FspBI CTAG 6 cut(s) 260, 372, 1020, 1136, 1325, 1382
GluI GCNGC 5 cut(s) 33, 386, 425, 874, 1057
GsaI CCCAGC 1 cut(s) 1056
HaeIII GGCC 1 cut(s) 1167
HgaI GACGC 2 cut(s) 479, 773
HindIII AAGCTT 3 cut(s) 217, 404, 1091
HinfI GANTC 8 cut(s) 77, 208, 706, 847, 949, 1230, 1244, 1267
HphI GGTGA 3 cut(s) 773, 1138, 1264
Hpy166II GTNNAC 3 cut(s) 395, 1376, 1451
Hpy188I TCNGA 6 cut(s) 42, 519, 940, 996, 1255, 1368
Hpy8I GTNNAC 3 cut(s) 395, 1376, 1451
HpyAV CCTTC 7 cut(s) 164, 287, 536, 597, 605, 842, 890
HpyCH4III ACNGT 3 cut(s) 186, 734, 1075
HpyCH4V TGCA 8 cut(s) 35, 133, 191, 419, 427, 546, 657, 691
HpyF10VI GCNNNNNNNGC 2 cut(s) 328, 1048
HpyF3I CTNAG 6 cut(s) 56, 306, 531, 678, 859, 1289
Kzo9I GATC 5 cut(s) 72, 98, 668, 1237, 1425
LmnI GCTCC 3 cut(s) 336, 535, 1317
Lsp1109I GCAGC 5 cut(s) 19, 372, 411, 860, 1068
LweI GCATC 2 cut(s) 169, 678
MaeI CTAG 6 cut(s) 260, 372, 1020, 1136, 1325, 1382
MaeIII GTNAC 2 cut(s) 765, 1075
MalI GATC 5 cut(s) 74, 100, 670, 1239, 1427
MboI GATC 5 cut(s) 72, 98, 668, 1237, 1425
MboII GAAGA 5 cut(s) 620, 875, 983, 1187, 1424
MflI RGATCY 2 cut(s) 98, 1237
MhlI GDGCHC 2 cut(s) 333, 517
MlyI GAGTC 2 cut(s) 217, 700
MmeI TCCRAC 3 cut(s) 205, 235, 981
MroXI GAANNNNTTC 1 cut(s) 456
MseI TTAA 5 cut(s) 345, 1061, 1095, 1208, 1217
MspCI CTTAAG 1 cut(s) 1060
MspR9I CCNGG 2 cut(s) 390, 1164
Mva1269I GAATGC 2 cut(s) 806, 1221
MvaI CCWGG 2 cut(s) 390, 1164
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 2 cut(s) 328, 1048
NdeI CATATG 1 cut(s) 639
NdeII GATC 5 cut(s) 72, 98, 668, 1237, 1425
NlaIV GGNNCC 1 cut(s) 100
PaeR7I CTCGAG 1 cut(s) 560
PctI GAATGC 2 cut(s) 806, 1221
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 6 cut(s) 77, 847, 949, 1230, 1244, 1267
PflMI CCANNNNNTGG 1 cut(s) 902
PkrI GCNGC 5 cut(s) 34, 387, 426, 875, 1058
PleI GAGTC 2 cut(s) 216, 700
PpsI GAGTC 2 cut(s) 216, 700
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 388, 1162
PspFI CCCAGC 1 cut(s) 1052
PspGI CCWGG 2 cut(s) 388, 1162
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 1419
PstI CTGCAG 1 cut(s) 429
PstNI CAGNNNCTG 2 cut(s) 1048, 1079
PsuI RGATCY 2 cut(s) 98, 1237
RsaI GTAC 4 cut(s) 61, 68, 342, 716
RsaNI GTAC 4 cut(s) 60, 67, 341, 715
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 5 cut(s) 345, 1061, 1095, 1208, 1217
SatI GCNGC 5 cut(s) 33, 386, 425, 874, 1057
Sau3AI GATC 5 cut(s) 72, 98, 668, 1237, 1425
Sau96I GGNCC 1 cut(s) 1419
ScaI AGTACT 1 cut(s) 716
SchI GAGTC 2 cut(s) 217, 700
ScrFI CCNGG 2 cut(s) 390, 1164
SduI GDGCHC 2 cut(s) 333, 517
SfaNI GCATC 2 cut(s) 169, 678
SfcI CTRYAG 1 cut(s) 425
Sfr274I CTCGAG 1 cut(s) 560
SinI GGWCC 1 cut(s) 1419
SlaI CTCGAG 1 cut(s) 560
SmlI CTYRAG 9 cut(s) 407, 560, 743, 779, 788, 851, 953, 1060, 1262
SmoI CTYRAG 9 cut(s) 407, 560, 743, 779, 788, 851, 953, 1060, 1262
SsiI CCGC 1 cut(s) 502
SspI AATATT 2 cut(s) 236, 350
SspMI CTAG 6 cut(s) 260, 372, 1020, 1136, 1325, 1382
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 388, 1162
TaaI ACNGT 3 cut(s) 186, 734, 1075
TaqI TCGA 3 cut(s) 75, 561, 1390
TaqII GACCGA 1 cut(s) 264
TatI WGTACW 3 cut(s) 59, 340, 714
TfiI GAWTC 6 cut(s) 77, 847, 949, 1230, 1244, 1267
Tru1I TTAA 5 cut(s) 345, 1061, 1095, 1208, 1217
Tru9I TTAA 5 cut(s) 345, 1061, 1095, 1208, 1217
TscAI CASTG 2 cut(s) 678, 1115
TseI GCWGC 5 cut(s) 32, 385, 424, 873, 1056
TspDTI ATGAA 4 cut(s) 395, 566, 656, 965
TspRI CASTG 2 cut(s) 678, 1115
Van91I CCANNNNNTGG 1 cut(s) 902
Vha464I CTTAAG 1 cut(s) 1060
VpaK11BI GGWCC 1 cut(s) 1419
XagI CCTNNNNNAGG 2 cut(s) 530, 884
XapI RAATTY 4 cut(s) 110, 644, 660, 968
XbaI TCTAGA 2 cut(s) 1135, 1381
XhoI CTCGAG 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 6 cut(s) 260, 372, 1020, 1136, 1325, 1382
ZrmI AGTACT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.