Rmu_sc0001304.1_g000002

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001304.1
OG0004242
Physical Location & Seq
Reverse (-)
3992 .. 5395
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1404 bp
atggcagagggttttgtgccagagccagaaggaaatcaactgatcgaagatgtagctggaagatatgtttcagattttttatcaaggtccttcttccaacacaccagtgacaagtcacgactggtaatgcatgacctcatacatgatcttgcacaatctgtttctggcaaaacatttgttaggttggaggaaaattcagagaaccataaaaagtctacaaaggctcggcacttgtcatatacccgtggatctgaagatgtatttcagaaatttgaggcattcagtgaggtggattgtttacgaaccattctaccactagacccgttgcaaggattcaatgtgagtgacttatctgacaaggttccttgtgatatgatgccaaaattaagacttctgcgggtgctatcctttagtggttatctcatagaaaagttgccagattcaattggaaacttgaagcatctgcgatacctcaatctttcttattgtcaaatagaagagctacctgagccaacaagcagtctatacaatctgcagacattaattttattcagatgtcgatcgcttaccatgctgcctgcagacataggaaacctaaggaatttacggcatctcaatattcagggaactaggctgaaaagtatgcctttgggaatgggaagattggttaaccttcaaatactgtcagattttattgtggggaaagccatcggaaaaaggattgcagaattgaaggacttgtcacatcttccaggctcagtttctattttgtgtttgcatgatgtcagcagtgttagggatgcaattgaggccagattagatgttaagatgcaccttgatgatttagttttggaatggagcagcagtactgatggttcgcgaaatgaagagatcgagacagaggtacttgatgcactgcagcctcatgaaaacctgaagaaactcaccattaagtattatggaggtacagaatttccatattggatggggaatcctttgttcactaacatggcgtacttgcatctctatggttgcataaaatgcacatctttaccaccccttggccaactaccttctcttaaagatctcataattgaagcaatgcatggaatacagcttgtaggaatggatttttttggggatgatgatggttccacaattttttccccctctttagagactttgaaatttgaggacatgaaagaatgggagaaatggtcatctttagaagatgaaaagattaaaggatttcccagccttcgggagctttccatatttagatgtcctaagttaacaaagttgatttgcttctctagaaaagctgctcatcaagaactgcggagcaataactaccttctcacagaatcctgcacttgtgaatttggaacctgtagaattccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

467

Amino Acids

53.08

Weight (kDa)

5.83

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 1 - 51 7.7e-14 Disease resistance protein Winged helix domain
LRR_14 PF23598 126 - 369 6.8e-28 Leucine-rich repeat region
LRR_Zer-1 PF25013 150 - 210 7.6e-06 Zer-1-like, Leucine-rich repeats
LRR_R13L1-DRL21 PF25019 241 - 368 1e-39 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1061
AccI GTMKAC 1 cut(s) 215
AccII CGCG 1 cut(s) 880
AciI CCGC 2 cut(s) 397, 1339
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 1063
AcsI RAATTY 7 cut(s) 193, 269, 601, 971, 1187, 1379, 1395
AcuI CTGAAG 2 cut(s) 273, 956
AfaI GTAC 4 cut(s) 868, 906, 967, 1016
AfiI CCNNNNNNNGG 3 cut(s) 329, 1061, 1260
AgsI TTSAA 7 cut(s) 337, 444, 457, 677, 733, 1097, 1186
AjnI CCWGG 1 cut(s) 751
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 5 cut(s) 56, 502, 1117, 1267, 1322
AluI AGCT 5 cut(s) 56, 502, 1117, 1267, 1322
Alw26I GTCTC 2 cut(s) 890, 1172
AlwI GGATC 1 cut(s) 256
AoxI GGCC 2 cut(s) 810, 1063
ApeKI GCWGC 4 cut(s) 574, 861, 919, 1322
ApoI RAATTY 7 cut(s) 193, 269, 601, 971, 1187, 1379, 1395
AseI ATTAAT 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 937
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 596
BalI TGGCCA 1 cut(s) 1065
BbvI GCAGC 4 cut(s) 561, 873, 931, 1309
BccI CCATC 4 cut(s) 716, 866, 979, 1142
BceAI ACGGC 1 cut(s) 623
BciT130I CCWGG 1 cut(s) 753
BcoDI GTCTC 2 cut(s) 890, 1172
BfaI CTAG 3 cut(s) 317, 630, 1314
BfmI CTRYAG 4 cut(s) 533, 579, 917, 1390
BglII AGATCT 1 cut(s) 1084
BisI GCNGC 4 cut(s) 575, 862, 920, 1323
BlsI GCNGC 4 cut(s) 576, 863, 921, 1324
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 1 cut(s) 753
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 363, 1153, 1387
BmrFI CCNGG 1 cut(s) 753
BmsI GCATC 7 cut(s) 366, 469, 619, 790, 819, 901, 1030
Bpu10I CCTNAGC 1 cut(s) 507
BsaBI GATNNNNATC 1 cut(s) 559
BsaJI CCNNGG 2 cut(s) 244, 1060
BsaXI ACNNNNNCTCC 4 cut(s) 850, 880, 1202, 1232
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 1061, 1260
Bse1I ACTGG 2 cut(s) 105, 126
Bse21I CCTNAGG 1 cut(s) 596
Bse3DI GCAATG 1 cut(s) 1107
Bse8I GATNNNNATC 1 cut(s) 559
BseBI CCWGG 1 cut(s) 753
BseDI CCNNGG 2 cut(s) 244, 1060
BseGI GGATG 3 cut(s) 805, 990, 1147
BseJI GATNNNNATC 1 cut(s) 559
BseLI CCNNNNNNNGG 3 cut(s) 329, 1061, 1260
BseMI GCAATG 1 cut(s) 1107
BseMII CTCAG 2 cut(s) 498, 771
BseNI ACTGG 2 cut(s) 105, 126
BseXI GCAGC 4 cut(s) 561, 873, 931, 1309
BseYI CCCAGC 1 cut(s) 1253
BsgI GTGCAG 1 cut(s) 1354
Bsh1236I CGCG 1 cut(s) 880
Bsh1285I CGRYCG 1 cut(s) 563
BshFI GGCC 2 cut(s) 812, 1065
BsiEI CGRYCG 1 cut(s) 563
BslI CCNNNNNNNGG 3 cut(s) 329, 1061, 1260
BsmAI GTCTC 2 cut(s) 890, 1172
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 2 cut(s) 812, 1065
Bsp143I GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
Bsp68I TCGCGA 1 cut(s) 880
BspACI CCGC 2 cut(s) 397, 1339
BspANI GGCC 2 cut(s) 812, 1065
BspCNI CTCAG 2 cut(s) 499, 770
BspFNI CGCG 1 cut(s) 880
BspHI TCATGA 1 cut(s) 925
BspLI GGNNCC 3 cut(s) 363, 1153, 1387
BspMAI CTGCAG 3 cut(s) 537, 583, 921
BspPI GGATC 1 cut(s) 256
BspQI GCTCTTC 1 cut(s) 492
BsrDI GCAATG 1 cut(s) 1107
BsrI ACTGG 2 cut(s) 105, 126
BssECI CCNNGG 2 cut(s) 244, 1060
BssMI GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
BssT1I CCWWGG 1 cut(s) 1060
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 1 cut(s) 684
Bst6I CTCTTC 2 cut(s) 492, 882
BstAPI GCANNNNNTGC 1 cut(s) 1041
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 4 cut(s) 507, 596, 757, 1287
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 3 cut(s) 805, 990, 1147
BstFNI CGCG 1 cut(s) 880
BstKTI GATC 6 cut(s) 45, 148, 251, 563, 894, 1087
BstMAI GTCTC 2 cut(s) 890, 1172
BstMBI GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
BstMCI CGRYCG 1 cut(s) 563
BstMWI GCNNNNNNNGC 4 cut(s) 508, 571, 809, 1041
BstNI CCWGG 1 cut(s) 753
BstSCI CCNGG 1 cut(s) 751
BstSFI CTRYAG 4 cut(s) 533, 579, 917, 1390
BstUI CGCG 1 cut(s) 880
BstV1I GCAGC 4 cut(s) 561, 873, 931, 1309
BstX2I RGATCY 2 cut(s) 248, 1084
BstYI RGATCY 2 cut(s) 248, 1084
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 2 cut(s) 812, 1065
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 805, 990, 1147
BtsI GCAGTG 2 cut(s) 796, 914
BtsIMutI CAGTG 4 cut(s) 112, 289, 796, 914
BtuMI TCGCGA 1 cut(s) 880
Cac8I GCNNGC 1 cut(s) 579
CciI TCATGA 1 cut(s) 925
Cfr13I GGNCC 1 cut(s) 87
Csp6I GTAC 4 cut(s) 867, 905, 966, 1015
CviAII CATG 8 cut(s) 131, 143, 571, 779, 926, 1009, 1106, 1198
CviQI GTAC 4 cut(s) 867, 905, 966, 1015
DdeI CTNAG 4 cut(s) 507, 596, 757, 1287
DpnI GATC 6 cut(s) 44, 147, 250, 562, 893, 1086
DpnII GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
EaeI YGGCCR 1 cut(s) 1063
Eam1104I CTCTTC 2 cut(s) 492, 882
EarI CTCTTC 2 cut(s) 492, 882
Eco130I CCWWGG 1 cut(s) 1060
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 273, 956
Eco81I CCTNAGG 1 cut(s) 596
EcoO109I RGGNCCY 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 1395
EcoRII CCWGG 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 1060
EcoT22I ATGCAT 2 cut(s) 132, 1107
ErhI CCWWGG 1 cut(s) 1060
FaeI CATG 8 cut(s) 134, 146, 574, 782, 929, 1012, 1109, 1201
FalI AAGNNNNNCTT 2 cut(s) 631, 663
FatI CATG 8 cut(s) 130, 142, 570, 778, 925, 1008, 1105, 1197
FauI CCCGC 1 cut(s) 390
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 4 cut(s) 575, 862, 920, 1323
FokI GGATG 3 cut(s) 812, 997, 1154
Fsp4HI GCNGC 4 cut(s) 575, 862, 920, 1323
FspBI CTAG 3 cut(s) 317, 630, 1314
GluI GCNGC 4 cut(s) 575, 862, 920, 1323
GsaI CCCAGC 1 cut(s) 1257
HaeIII GGCC 2 cut(s) 812, 1065
Hin1II CATG 8 cut(s) 134, 146, 574, 782, 929, 1012, 1109, 1201
HincII GTYRAC 2 cut(s) 670, 1293
HindII GTYRAC 2 cut(s) 670, 1293
HinfI GANTC 4 cut(s) 333, 440, 991, 1364
HpaI GTTAAC 2 cut(s) 670, 1293
HphI GGTGA 1 cut(s) 937
Hpy166II GTNNAC 5 cut(s) 216, 299, 670, 1002, 1293
Hpy188I TCNGA 8 cut(s) 73, 199, 253, 267, 355, 554, 688, 713
Hpy188III TCNNGA 7 cut(s) 117, 879, 895, 926, 1262, 1314, 1331
Hpy8I GTNNAC 5 cut(s) 216, 299, 670, 1002, 1293
HpyAV CCTTC 7 cut(s) 23, 100, 683, 727, 1083, 1268, 1364
HpyCH4III ACNGT 1 cut(s) 684
HpyF10VI GCNNNNNNNGC 4 cut(s) 508, 571, 809, 1041
HpyF3I CTNAG 4 cut(s) 507, 596, 757, 1287
Hsp92II CATG 8 cut(s) 134, 146, 574, 782, 929, 1012, 1109, 1201
KspAI GTTAAC 2 cut(s) 670, 1293
Kzo9I GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 3 cut(s) 858, 1264, 1341
Lsp1109I GCAGC 4 cut(s) 561, 873, 931, 1309
LweI GCATC 7 cut(s) 366, 469, 619, 790, 819, 901, 1030
MaeI CTAG 3 cut(s) 317, 630, 1314
MaeIII GTNAC 4 cut(s) 107, 114, 344, 741
MalI GATC 6 cut(s) 44, 147, 250, 562, 893, 1086
MboI GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
MfeI CAATTG 2 cut(s) 444, 804
MflI RGATCY 2 cut(s) 248, 1084
MlsI TGGCCA 1 cut(s) 1065
MluNI TGGCCA 1 cut(s) 1065
MmeI TCCRAC 2 cut(s) 121, 165
Mox20I TGGCCA 1 cut(s) 1065
Mph1103I ATGCAT 2 cut(s) 132, 1107
MscI TGGCCA 1 cut(s) 1065
MseI TTAA 8 cut(s) 386, 542, 669, 825, 951, 1080, 1242, 1292
MslI CAYNNNNRTG 4 cut(s) 105, 837, 1007, 1026
Msp20I TGGCCA 1 cut(s) 1065
MspR9I CCNGG 1 cut(s) 753
MunI CAATTG 2 cut(s) 444, 804
Mva1269I GAATGC 1 cut(s) 278
MvaI CCWGG 1 cut(s) 753
MvnI CGCG 1 cut(s) 880
MwoI GCNNNNNNNGC 4 cut(s) 508, 571, 809, 1041
NdeII GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
NlaIII CATG 8 cut(s) 134, 146, 574, 782, 929, 1012, 1109, 1201
NlaIV GGNNCC 3 cut(s) 363, 1153, 1387
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 4 cut(s) 107, 114, 344, 741
NruI TCGCGA 1 cut(s) 880
NsiI ATGCAT 2 cut(s) 132, 1107
OliI CACNNNNGTG 1 cut(s) 105
PagI TCATGA 1 cut(s) 925
PciSI GCTCTTC 1 cut(s) 492
PctI GAATGC 1 cut(s) 278
PfeI GAWTC 4 cut(s) 333, 440, 991, 1364
PflMI CCANNNNNTGG 1 cut(s) 1061
PkrI GCNGC 4 cut(s) 576, 863, 921, 1324
Ple19I CGATCG 1 cut(s) 563
PpuMI RGGWCCY 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 542
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 751
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 1 cut(s) 751
PspN4I GGNNCC 3 cut(s) 363, 1153, 1387
PspPI GGNCC 1 cut(s) 87
PspPPI RGGWCCY 1 cut(s) 87
PsrI GAACNNNNNNTAC 2 cut(s) 859, 891
PstI CTGCAG 3 cut(s) 537, 583, 921
PsuI RGATCY 2 cut(s) 248, 1084
PvuI CGATCG 1 cut(s) 563
RruI TCGCGA 1 cut(s) 880
RsaI GTAC 4 cut(s) 868, 906, 967, 1016
RsaNI GTAC 4 cut(s) 867, 905, 966, 1015
RseI CAYNNNNRTG 4 cut(s) 105, 837, 1007, 1026
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 8 cut(s) 386, 542, 669, 825, 951, 1080, 1242, 1292
SatI GCNGC 4 cut(s) 575, 862, 920, 1323
Sau3AI GATC 6 cut(s) 42, 145, 248, 560, 891, 1084
Sau96I GGNCC 1 cut(s) 87
ScaI AGTACT 1 cut(s) 868
ScrFI CCNGG 1 cut(s) 753
SfaNI GCATC 7 cut(s) 366, 469, 619, 790, 819, 901, 1030
SfcI CTRYAG 4 cut(s) 533, 579, 917, 1390
SinI GGWCC 1 cut(s) 87
SmiMI CAYNNNNRTG 4 cut(s) 105, 837, 1007, 1026
SsiI CCGC 2 cut(s) 397, 1339
SspI AATATT 1 cut(s) 619
SspMI CTAG 3 cut(s) 317, 630, 1314
StyD4I CCNGG 1 cut(s) 751
StyI CCWWGG 1 cut(s) 1060
TaaI ACNGT 1 cut(s) 684
TaqI TCGA 3 cut(s) 45, 559, 894
TatI WGTACW 1 cut(s) 866
TfiI GAWTC 4 cut(s) 333, 440, 991, 1364
Tru1I TTAA 8 cut(s) 386, 542, 669, 825, 951, 1080, 1242, 1292
Tru9I TTAA 8 cut(s) 386, 542, 669, 825, 951, 1080, 1242, 1292
TscAI CASTG 4 cut(s) 112, 289, 796, 921
TseFI GTSAC 4 cut(s) 107, 114, 344, 741
TseI GCWGC 4 cut(s) 574, 861, 919, 1322
Tsp45I GTSAC 4 cut(s) 107, 114, 344, 741
TspDTI ATGAA 4 cut(s) 900, 942, 1214, 1248
TspRI CASTG 4 cut(s) 112, 289, 796, 921
Van91I CCANNNNNTGG 1 cut(s) 1061
VpaK11BI GGWCC 1 cut(s) 87
VspI ATTAAT 1 cut(s) 542
XapI RAATTY 7 cut(s) 193, 269, 601, 971, 1187, 1379, 1395
XbaI TCTAGA 1 cut(s) 1313
XmiI GTMKAC 1 cut(s) 215
XspI CTAG 3 cut(s) 317, 630, 1314
ZrmI AGTACT 1 cut(s) 868
Zsp2I ATGCAT 2 cut(s) 132, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.