Rmu_sc0001304.1_g000003

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001304.1
OG0004242
Physical Location & Seq
Reverse (-)
5564 .. 6559
996 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 996 bp
atgtcattgatttcttccttaagtggaaaatcgatgattccatgccaggacatattggatgacatcgccaatgatgctctcgaatcacaaaacagcaaacctgaggtatggaattctaagatcaaagaagggatagactttaagatgaaagacattgctaatactcttaatccctttagggaacgtgtagagtcaaaaacgataaagattatagatgtattggaagagattgcaaagcagaaagatattcttcgtttgagagaagattttggcagtatagaatcaggttttgacggattgcccacgactccaatggtgggtgataaatctcatgtttatggtagagaatctgataaggaggagataattactttgctgaaatcgagtaaaaatgatcgtgaggtttctataattccaattgtaggcatgggagacaatgggaagactactcttgctcagcttgtgtataatgatgccaaagtgagtagctattttaatttgagagcatgggcttgtgtctctgatgtatttgatgtgcaaaggataacaaaaacacttgttgagtccgcgacaaaatcaagttgtagcacaaacaatttggagttacttcaagaacaactgaaaatacggttgaagaacaagaaatttctttttgttctggatgatgtctggaacgacaagtatgaaagctgggataagttaagagtccccttcacagttggtgcaccagggaataaaatcatgatcacgacacgaaacaaaacagttgcatcacttatggggactgttcctacttactgtttggaggggctttcggacagtgattgttggttattattcgaacaaattgtgtttcagaacaggaatttagatgcctatccaaagttgaaagtgattggcaagaaaatagcatacaagtgtaaagggttgccattggctgcaaaggcactaggaggtctcctgcgtgctgaaccggaactagatgagaattactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

331

Amino Acids

37.19

Weight (kDa)

6.11

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 116 - 288 2.2e-34 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 569
AciI CCGC 1 cut(s) 567
AcsI RAATTY 3 cut(s) 112, 644, 865
AfiI CCNNNNNNNGG 2 cut(s) 317, 422
AflII CTTAAG 1 cut(s) 19
AflIII ACRYGT 1 cut(s) 184
AgsI TTSAA 3 cut(s) 611, 634, 889
AjnI CCWGG 2 cut(s) 45, 727
AluBI AGCT 3 cut(s) 460, 489, 690
AluI AGCT 3 cut(s) 460, 489, 690
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 3 cut(s) 426, 523, 962
Alw44I GTGCAC 1 cut(s) 723
ApaLI GTGCAC 1 cut(s) 723
ApeKI GCWGC 1 cut(s) 938
ApoI RAATTY 3 cut(s) 112, 644, 865
AsuHPI GGTGA 1 cut(s) 332
AsuII TTCGAA 1 cut(s) 840
AxyI CCTNAGG 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 727
BbsI GAAGAC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 1 cut(s) 925
BciT130I CCWGG 2 cut(s) 47, 729
BclI TGATCA 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 426, 523, 962
BfaI CTAG 3 cut(s) 950, 980, 994
BfrI CTTAAG 1 cut(s) 19
BisI GCNGC 1 cut(s) 939
BlpI GCTNAGC 1 cut(s) 456
BlsI GCNGC 1 cut(s) 940
Bme1390I CCNGG 2 cut(s) 47, 729
BmrFI CCNGG 2 cut(s) 47, 729
BmsI GCATC 4 cut(s) 64, 463, 779, 862
BpiI GAAGAC 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 456
Bpu14I TTCGAA 1 cut(s) 840
Bsa29I ATCGAT 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 876
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 1 cut(s) 728
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 422
Bse21I CCTNAGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 2 cut(s) 47, 729
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 728
BseGI GGATG 2 cut(s) 64, 667
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 2 cut(s) 317, 422
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 2 cut(s) 93, 470
BseRI GAGGAG 1 cut(s) 374
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 1 cut(s) 925
BseYI CCCAGC 1 cut(s) 690
Bsh1236I CGCG 1 cut(s) 569
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 727
BsiSI CCGG 1 cut(s) 974
BslFI GGGAC 2 cut(s) 692, 796
BslI CCNNNNNNNGG 2 cut(s) 317, 422
BsmAI GTCTC 3 cut(s) 426, 523, 962
BsmFI GGGAC 2 cut(s) 692, 796
Bso31I GGTCTC 1 cut(s) 962
Bsp119I TTCGAA 1 cut(s) 840
Bsp1286I GDGCHC 1 cut(s) 727
Bsp143I GATC 3 cut(s) 120, 394, 744
Bsp1720I GCTNAGC 1 cut(s) 456
BspACI CCGC 1 cut(s) 567
BspCNI CTCAG 2 cut(s) 94, 469
BspDI ATCGAT 1 cut(s) 32
BspFNI CGCG 1 cut(s) 569
BspHI TCATGA 1 cut(s) 741
BspT104I TTCGAA 1 cut(s) 840
BspTI CTTAAG 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 962
BsrDI GCAATG 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 728
BssMI GATC 3 cut(s) 120, 394, 744
Bst2UI CCWGG 2 cut(s) 47, 729
Bst4CI ACNGT 6 cut(s) 630, 718, 766, 787, 800, 821
Bst6I CTCTTC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 19
BstBI TTCGAA 1 cut(s) 840
BstC8I GCNNGC 1 cut(s) 966
BstDEI CTNAG 3 cut(s) 102, 117, 456
BstF5I GGATG 2 cut(s) 64, 667
BstFNI CGCG 1 cut(s) 569
BstKTI GATC 3 cut(s) 123, 397, 747
BstMAI GTCTC 3 cut(s) 426, 523, 962
BstMBI GATC 3 cut(s) 120, 394, 744
BstMWI GCNNNNNNNGC 2 cut(s) 74, 944
BstNI CCWGG 2 cut(s) 47, 729
BstSCI CCNGG 2 cut(s) 45, 727
BstSLI GKGCMC 1 cut(s) 727
BstUI CGCG 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 925
BstV2I GAAGAC 1 cut(s) 449
Bsu15I ATCGAT 1 cut(s) 32
Bsu36I CCTNAGG 1 cut(s) 102
BsuTUI ATCGAT 1 cut(s) 32
BtgZI GCGATG 1 cut(s) 49
BtsCI GGATG 2 cut(s) 64, 667
BtsIMutI CAGTG 1 cut(s) 826
Cac8I GCNNGC 1 cut(s) 966
CciI TCATGA 1 cut(s) 741
ClaI ATCGAT 1 cut(s) 32
CviAII CATG 5 cut(s) 42, 332, 427, 507, 742
CviJI RGCY 6 cut(s) 460, 489, 512, 690, 811, 938
CviKI_1 RGCY 6 cut(s) 460, 489, 512, 690, 811, 938
DdeI CTNAG 3 cut(s) 102, 117, 456
DpnI GATC 3 cut(s) 122, 396, 746
DpnII GATC 3 cut(s) 120, 394, 744
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 962
Eco81I CCTNAGG 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 45, 727
FaeI CATG 5 cut(s) 45, 335, 430, 510, 745
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 2 cut(s) 692, 796
FatI CATG 5 cut(s) 41, 331, 426, 506, 741
FbaI TGATCA 1 cut(s) 744
Fnu4HI GCNGC 1 cut(s) 939
FokI GGATG 2 cut(s) 71, 674
Fsp4HI GCNGC 1 cut(s) 939
FspBI CTAG 3 cut(s) 950, 980, 994
GluI GCNGC 1 cut(s) 939
GsaI CCCAGC 1 cut(s) 694
HapII CCGG 1 cut(s) 974
Hin1II CATG 5 cut(s) 45, 335, 430, 510, 745
HinfI GANTC 8 cut(s) 37, 83, 191, 281, 307, 347, 563, 705
HpaII CCGG 1 cut(s) 974
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 725
Hpy188I TCNGA 4 cut(s) 352, 523, 817, 858
Hpy188III TCNNGA 7 cut(s) 80, 398, 611, 659, 670, 742, 748
Hpy8I GTNNAC 1 cut(s) 725
HpyAV CCTTC 2 cut(s) 122, 721
HpyCH4III ACNGT 6 cut(s) 630, 718, 766, 787, 800, 821
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 5 cut(s) 233, 538, 725, 770, 941
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 944
HpyF3I CTNAG 3 cut(s) 102, 117, 456
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 5 cut(s) 45, 335, 430, 510, 745
Ksp22I TGATCA 1 cut(s) 744
Kzo9I GATC 3 cut(s) 120, 394, 744
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 4 cut(s) 64, 463, 779, 862
MaeI CTAG 3 cut(s) 950, 980, 994
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 1 cut(s) 603
MalI GATC 3 cut(s) 122, 396, 746
MboI GATC 3 cut(s) 120, 394, 744
MboII GAAGA 6 cut(s) 6, 236, 242, 275, 454, 646
MfeI CAATTG 1 cut(s) 417
MhlI GDGCHC 1 cut(s) 727
MlyI GAGTC 4 cut(s) 200, 301, 572, 714
MnlI CCTC 5 cut(s) 97, 352, 394, 799, 947
MseI TTAA 5 cut(s) 20, 141, 168, 495, 701
MslI CAYNNNNRTG 2 cut(s) 336, 916
MspCI CTTAAG 1 cut(s) 19
MspI CCGG 1 cut(s) 974
MspR9I CCNGG 2 cut(s) 47, 729
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 2 cut(s) 47, 729
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 2 cut(s) 74, 944
NdeII GATC 3 cut(s) 120, 394, 744
NlaIII CATG 5 cut(s) 45, 335, 430, 510, 745
NspV TTCGAA 1 cut(s) 840
PagI TCATGA 1 cut(s) 741
PfeI GAWTC 4 cut(s) 37, 83, 281, 347
PkrI GCNGC 1 cut(s) 940
PleI GAGTC 4 cut(s) 199, 301, 571, 713
PpsI GAGTC 4 cut(s) 199, 301, 571, 713
Psp6I CCWGG 2 cut(s) 45, 727
PspFI CCCAGC 1 cut(s) 690
PspGI CCWGG 2 cut(s) 45, 727
RseI CAYNNNNRTG 2 cut(s) 336, 916
SaqAI TTAA 5 cut(s) 20, 141, 168, 495, 701
SatI GCNGC 1 cut(s) 939
Sau3AI GATC 3 cut(s) 120, 394, 744
SchI GAGTC 4 cut(s) 200, 301, 572, 714
ScrFI CCNGG 2 cut(s) 47, 729
SduI GDGCHC 1 cut(s) 727
SetI ASST 9 cut(s) 103, 108, 187, 289, 405, 462, 491, 692, 958
SfaNI GCATC 4 cut(s) 64, 463, 779, 862
SfuI TTCGAA 1 cut(s) 840
SmiMI CAYNNNNRTG 2 cut(s) 336, 916
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
SsiI CCGC 1 cut(s) 567
SspMI CTAG 3 cut(s) 950, 980, 994
StyD4I CCNGG 2 cut(s) 45, 727
TaaI ACNGT 6 cut(s) 630, 718, 766, 787, 800, 821
TaiI ACGT 1 cut(s) 187
TaqI TCGA 4 cut(s) 32, 81, 383, 840
TfiI GAWTC 4 cut(s) 37, 83, 281, 347
Tru1I TTAA 5 cut(s) 20, 141, 168, 495, 701
Tru9I TTAA 5 cut(s) 20, 141, 168, 495, 701
TscAI CASTG 1 cut(s) 826
TseI GCWGC 1 cut(s) 938
TspDTI ATGAA 2 cut(s) 161, 699
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 1 cut(s) 826
Vha464I CTTAAG 1 cut(s) 19
VneI GTGCAC 1 cut(s) 723
XapI RAATTY 3 cut(s) 112, 644, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XspI CTAG 3 cut(s) 950, 980, 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.