Rmu_sc0002115.1_g000025

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002115.1
OG0003273
Physical Location & Seq
Forward (+)
143683 .. 144974
1292 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
atgatgatggatcagagcaagtccatctccacttctttatttatgcttcttcttcttctccttcaaattttgcactcaacacgcattacagctcaagaagttgaagaagaaagagagtttgattatctgaaagacagtgagaagggaccaaagtattggggagagcttagggaagaatgggcagcatgtaaacatgggacactgcaatctccggtagatttgctgagtgagaatgtgcaagtacgcctaaacaaaggggaggtgagactcaagtacaagcctgcaaatgcaacaatcagaaatagaggccatgacatttcggtccagtggaatctaggtgatgctggatccgtagagattaatggcacagattacttactacaacaatgccactggcactcaccatccgagcatacaattgatggtataagatttgacatggagcttcatatggtacacctaatccctgaggcaaatgtcgaaaacaacatagttgtggttacatacctctacaaacttggcaagcctgataagtttctgtcggaggtaagcaaggatatccatgccatgattgatgaagaggaagagagacacagaggggtggttgatccaagagatgtgaagaagggtggcaaaaaattttacaagtacataggctcactcactgtacctccttgcactgaaggagtaacttggatcatccacaaaaaggtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

238

Amino Acids

27.4

Weight (kDa)

6.0

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 49 - 237 1.1e-31 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AclWI GGATC 5 cut(s) 18, 342, 355, 602, 704
AcsI RAATTY 2 cut(s) 66, 638
AcuI CTGAAG 1 cut(s) 702
AfaI GTAC 5 cut(s) 243, 275, 456, 650, 669
AfiI CCNNNNNNNGG 1 cut(s) 709
AgsI TTSAA 2 cut(s) 65, 104
AluBI AGCT 3 cut(s) 92, 166, 445
AluI AGCT 3 cut(s) 92, 166, 445
Alw26I GTCTC 2 cut(s) 259, 583
AlwI GGATC 5 cut(s) 18, 342, 355, 602, 704
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 2 cut(s) 66, 638
AseI ATTAAT 1 cut(s) 360
AspS9I GGNCC 2 cut(s) 146, 322
AsuHPI GGTGA 3 cut(s) 274, 350, 393
AvaII GGWCC 2 cut(s) 146, 322
AxyI CCTNAGG 1 cut(s) 468
BamHI GGATCC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 194
BccI CCATC 3 cut(s) 32, 412, 416
BcoDI GTCTC 2 cut(s) 259, 583
BfaI CTAG 1 cut(s) 335
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme18I GGWCC 2 cut(s) 146, 322
BmgT120I GGNCC 2 cut(s) 146, 322
BmiI GGNNCC 2 cut(s) 147, 349
BmsI GCATC 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 167
BpuEI CTTGAG 2 cut(s) 78, 254
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 1 cut(s) 709
Bse1I ACTGG 2 cut(s) 325, 398
Bse21I CCTNAGG 1 cut(s) 468
BseGI GGATG 2 cut(s) 404, 699
BseLI CCNNNNNNNGG 1 cut(s) 709
BseMII CTCAG 2 cut(s) 215, 459
BseNI ACTGG 2 cut(s) 325, 398
BseXI GCAGC 1 cut(s) 194
BshFI GGCC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 212
BslFI GGGAC 2 cut(s) 159, 211
BslI CCNNNNNNNGG 1 cut(s) 709
BsmAI GTCTC 2 cut(s) 259, 583
BsmFI GGGAC 2 cut(s) 159, 211
BsnI GGCC 1 cut(s) 309
Bsp143I GATC 4 cut(s) 10, 347, 607, 696
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 2 cut(s) 216, 460
BspLI GGNNCC 2 cut(s) 147, 349
BspPI GGATC 5 cut(s) 18, 342, 355, 602, 704
BsrI ACTGG 2 cut(s) 325, 398
BssMI GATC 4 cut(s) 10, 347, 607, 696
Bst4CI ACNGT 2 cut(s) 137, 667
Bst6I CTCTTC 2 cut(s) 573, 579
BstC8I GCNNGC 2 cut(s) 282, 524
BstDEI CTNAG 3 cut(s) 167, 224, 468
BstF5I GGATG 2 cut(s) 404, 699
BstKTI GATC 4 cut(s) 13, 350, 610, 699
BstMAI GTCTC 2 cut(s) 259, 583
BstMBI GATC 4 cut(s) 10, 347, 607, 696
BstNSI RCATGY 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 156
BstYI RGATCY 1 cut(s) 347
Bsu36I CCTNAGG 1 cut(s) 468
BsuRI GGCC 1 cut(s) 309
BtsCI GGATG 2 cut(s) 404, 699
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 6 cut(s) 142, 200, 332, 391, 663, 678
Cac8I GCNNGC 2 cut(s) 282, 524
Cfr13I GGNCC 2 cut(s) 146, 322
Csp6I GTAC 5 cut(s) 242, 274, 455, 649, 668
CviAII CATG 6 cut(s) 186, 194, 311, 439, 563, 568
CviJI RGCY 7 cut(s) 92, 166, 280, 309, 445, 526, 657
CviKI_1 RGCY 7 cut(s) 92, 166, 280, 309, 445, 526, 657
CviQI GTAC 5 cut(s) 242, 274, 455, 649, 668
DdeI CTNAG 3 cut(s) 167, 224, 468
DpnI GATC 4 cut(s) 12, 349, 609, 698
DpnII GATC 4 cut(s) 10, 347, 607, 696
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
Eam1104I CTCTTC 2 cut(s) 573, 579
EarI CTCTTC 2 cut(s) 573, 579
Eco32I GATATC 1 cut(s) 559
Eco47I GGWCC 2 cut(s) 146, 322
Eco57I CTGAAG 1 cut(s) 702
Eco81I CCTNAGG 1 cut(s) 468
EcoRV GATATC 1 cut(s) 559
FaeI CATG 6 cut(s) 189, 197, 314, 442, 566, 571
FaqI GGGAC 2 cut(s) 159, 211
FatI CATG 6 cut(s) 185, 193, 310, 438, 562, 567
FauNDI CATATG 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 2 cut(s) 391, 686
Fsp4HI GCNGC 1 cut(s) 183
FspBI CTAG 1 cut(s) 335
GluI GCNGC 1 cut(s) 183
HaeIII GGCC 1 cut(s) 309
HapII CCGG 1 cut(s) 212
Hin1II CATG 6 cut(s) 189, 197, 314, 442, 566, 571
HinfI GANTC 2 cut(s) 267, 331
HpaII CCGG 1 cut(s) 212
HphI GGTGA 3 cut(s) 274, 350, 393
Hpy166II GTNNAC 2 cut(s) 191, 457
Hpy188I TCNGA 5 cut(s) 15, 129, 299, 409, 544
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 2 cut(s) 191, 457
HpyAV CCTTC 4 cut(s) 71, 136, 619, 677
HpyCH4III ACNGT 2 cut(s) 137, 667
HpyCH4V TGCA 6 cut(s) 73, 205, 238, 284, 290, 678
HpyF3I CTNAG 3 cut(s) 167, 224, 468
Hsp92II CATG 6 cut(s) 189, 197, 314, 442, 566, 571
Kzo9I GATC 4 cut(s) 10, 347, 607, 696
LmnI GCTCC 1 cut(s) 442
LpnPI CCDG 7 cut(s) 225, 294, 330, 338, 379, 480, 540
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 1 cut(s) 331
MaeI CTAG 1 cut(s) 335
MaeIII GTNAC 2 cut(s) 499, 688
MalI GATC 4 cut(s) 12, 349, 609, 698
MboI GATC 4 cut(s) 10, 347, 607, 696
MboII GAAGA 9 cut(s) 41, 44, 47, 116, 119, 185, 590, 596, 634
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 1 cut(s) 347
MluCI AATT 3 cut(s) 66, 417, 638
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 8 cut(s) 253, 299, 463, 518, 538, 574, 590, 681
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 494
MspI CCGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 417
NdeI CATATG 1 cut(s) 450
NdeII GATC 4 cut(s) 10, 347, 607, 696
NlaIII CATG 6 cut(s) 189, 197, 314, 442, 566, 571
NlaIV GGNNCC 2 cut(s) 147, 349
NspI RCATGY 1 cut(s) 189
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 1 cut(s) 184
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
PshBI ATTAAT 1 cut(s) 360
PspN4I GGNNCC 2 cut(s) 147, 349
PspPI GGNCC 2 cut(s) 146, 322
PsuI RGATCY 1 cut(s) 347
RsaI GTAC 5 cut(s) 243, 275, 456, 650, 669
RsaNI GTAC 5 cut(s) 242, 274, 455, 649, 668
RseI CAYNNNNRTG 1 cut(s) 494
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 4 cut(s) 10, 347, 607, 696
Sau96I GGNCC 2 cut(s) 146, 322
SchI GAGTC 1 cut(s) 261
SfaNI GCATC 1 cut(s) 331
SinI GGWCC 2 cut(s) 146, 322
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 2 cut(s) 93, 269
SmoI CTYRAG 2 cut(s) 93, 269
Sse9I AATT 3 cut(s) 66, 417, 638
SspMI CTAG 1 cut(s) 335
TaaI ACNGT 2 cut(s) 137, 667
TaqI TCGA 1 cut(s) 480
TaqII GACCGA 1 cut(s) 310
TasI AATT 3 cut(s) 66, 417, 638
TatI WGTACW 2 cut(s) 273, 648
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 6 cut(s) 142, 207, 332, 398, 670, 685
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 437, 591
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 6 cut(s) 142, 207, 332, 398, 670, 685
VpaK11BI GGWCC 2 cut(s) 146, 322
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 2 cut(s) 66, 638
XceI RCATGY 1 cut(s) 189
XspI CTAG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.