Rmu_sc0002372.1_g000006

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002372.1
OG0001753
Physical Location & Seq
Forward (+)
28655 .. 34456
5802 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1416 bp
atggcttcttctaagccggttcgggtcgctgcgtgcattctatggcttctgttcctcacgcttccctcttcttcttcttcctctgaatcgccgactgagtattctctagttattggtgagaatgctactctgcaattatctaatggtgtgctggtgcaaaattctcctggtgctaagcctggaacttcactgttgtgtggaagagttcatgtatgtggattgtcaaggcttaaatatctggagaagtttgccgacacggtgaaggtgaagatatcaacaaattcgagctttcggatacggccttttgaggtttgcttccacagaaatacttctcgtgggataggaatgtgccctcacagccagtgggagaaagtttccaaggggtcctggtctggatccatgtcaccttatgagcgcaaactcttagatatacggacagtgggttcatccttagagagttttgaggtgtcccttgaagaagaattttttatatctcgcctagtattttttgtgtctggtgtaatcttgatgagtttggcaccttctctgagcaattcattggtattctattacggcagtggaatggcaataggggtggtccttgtactattgatcgtcctttttcaggggatgaagcttctcccaacaggtcggaagagttcgctcggaatttttttgttttcatctgtggttggtttgggatcttttctcctcagctacatcccagggctattgcgttcagtactaacagagcttggaattagcgaagagatgtataatcctttccaaacaaaaggaatgggcttgatatggagaaatactcatggacccagagtctcaaaagatactctatcaagtgcagggaagaaaatggtggagtacatggtgccattagcagagaaaactaaagacatggccgtgtctgcaagtaagaccactctgcattatgctggtcatgttgtggtagatttcaaggacaaggcgactgtggcaggttggactgcagcgcactatgcaactgaggcagcagtggagggaaccaaagcagctgcaagagtagttgccggagcagcagggtatgccggtcatgctgctgtggtgactggtgagaaagctgaggagttcactgctcggaagaaggaagaggcgaagagagaatatgaggccaagaaagcagctgaagcaaataggaccaagggttctgagactacttatcaaggaggagaatccaaatgctgggagcaagaggagacatggacgaaaccagttcagagggaatctcagcatatgcggagtggcagaccaactgaaaaaactagccagaaaccgctcaattacagagggagcgtgagagggcagtacctgggtgctgggaacaatcggcaggacttgaggtgtgataccgggagctgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

471

Amino Acids

51.32

Weight (kDa)

9.34

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 252 2.2e-24 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 983
AccB1I GGYRCC 2 cut(s) 538, 886
AccB7I CCANNNNNTGG 1 cut(s) 1236
AccBSI CCGCTC 1 cut(s) 1330
AciI CCGC 2 cut(s) 1291, 1328
AclWI GGATC 3 cut(s) 390, 403, 709
AcoI YGGCCR 1 cut(s) 915
AcsI RAATTY 4 cut(s) 160, 280, 482, 669
AcuI CTGAAG 1 cut(s) 1200
AfaI GTAC 4 cut(s) 606, 744, 881, 1361
AfiI CCNNNNNNNGG 2 cut(s) 625, 1236
AgsI TTSAA 2 cut(s) 476, 973
AhdI GACNNNNNGTC 1 cut(s) 833
AjnI CCWGG 5 cut(s) 166, 178, 386, 724, 1362
AleI CACNNNNGTG 1 cut(s) 193
AloI GAACNNNNNNTCC 4 cut(s) 427, 459, 1183, 1215
AluBI AGCT 8 cut(s) 288, 637, 717, 754, 1049, 1115, 1178, 1410
AluI AGCT 8 cut(s) 288, 637, 717, 754, 1049, 1115, 1178, 1410
Alw26I GTCTC 3 cut(s) 841, 1199, 1244
AlwI GGATC 3 cut(s) 390, 403, 709
AlwNI CAGNNNCTG 1 cut(s) 1363
AoxI GGCC 3 cut(s) 299, 915, 1164
ApeKI GCWGC 8 cut(s) 29, 1004, 1025, 1046, 1049, 1070, 1091, 1175
ApoI RAATTY 4 cut(s) 160, 280, 482, 669
Asp700I GAANNNNTTC 1 cut(s) 328
AspLEI GCGC 2 cut(s) 417, 1009
AspS9I GGNCC 4 cut(s) 384, 598, 827, 1191
AsuC2I CCSGG 1 cut(s) 1405
AsuHPI GGTGA 6 cut(s) 128, 271, 277, 396, 1111, 1118
AvaII GGWCC 4 cut(s) 384, 598, 827, 1191
BaeGI GKGCMC 1 cut(s) 353
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 538, 886
BauI CACGAG 1 cut(s) 333
BbvCI CCTCAGC 2 cut(s) 713, 1116
BbvI GCAGC 8 cut(s) 16, 1016, 1036, 1037, 1058, 1078, 1082, 1187
BceAI ACGGC 3 cut(s) 314, 589, 902
BciT130I CCWGG 5 cut(s) 168, 180, 388, 726, 1364
BciVI GTATCC 1 cut(s) 288
BcnI CCSGG 1 cut(s) 1405
BcoDI GTCTC 3 cut(s) 841, 1199, 1244
BfaI CTAG 3 cut(s) 107, 500, 1317
BfmI CTRYAG 1 cut(s) 1002
BfuAI ACCTGC 1 cut(s) 983
BfuI GTATCC 1 cut(s) 288
BisI GCNGC 8 cut(s) 30, 1005, 1026, 1047, 1050, 1071, 1092, 1176
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 8 cut(s) 31, 1006, 1027, 1048, 1051, 1072, 1093, 1177
BmcAI AGTACT 1 cut(s) 744
Bme1390I CCNGG 6 cut(s) 168, 180, 388, 726, 1364, 1405
Bme18I GGWCC 4 cut(s) 384, 598, 827, 1191
BmeRI GACNNNNNGTC 1 cut(s) 833
BmgT120I GGNCC 4 cut(s) 384, 598, 827, 1191
BmiI GGNNCC 6 cut(s) 385, 397, 540, 829, 888, 1039
BmrFI CCNGG 6 cut(s) 168, 180, 388, 726, 1364, 1405
BplI GAGNNNNNCTC 4 cut(s) 805, 837, 1264, 1296
BpmI CTGGAG 1 cut(s) 260
Bpu10I CCTNAGC 2 cut(s) 713, 1116
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 1411
BpuMI CCSGG 1 cut(s) 1405
BsaJI CCNNGG 5 cut(s) 378, 724, 725, 1194, 1363
Bsc4I CCNNNNNNNGG 2 cut(s) 625, 1236
Bse118I RCCGGY 2 cut(s) 16, 1082
Bse1I ACTGG 3 cut(s) 361, 1108, 1265
BseBI CCWGG 5 cut(s) 168, 180, 388, 726, 1364
BseDI CCNNGG 5 cut(s) 378, 724, 725, 1194, 1363
BseGI GGATG 3 cut(s) 446, 636, 720
BseLI CCNNNNNNNGG 2 cut(s) 625, 1236
BseMII CTCAG 7 cut(s) 87, 539, 727, 1011, 1107, 1194, 1295
BseNI ACTGG 3 cut(s) 361, 1108, 1265
BseRI GAGGAG 4 cut(s) 701, 1133, 1236, 1262
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 8 cut(s) 16, 1016, 1036, 1037, 1058, 1078, 1082, 1187
BseYI CCCAGC 2 cut(s) 1236, 1370
BsgI GTGCAG 1 cut(s) 879
BshFI GGCC 3 cut(s) 301, 917, 1166
BshNI GGYRCC 2 cut(s) 538, 886
BsiSI CCGG 4 cut(s) 17, 1065, 1083, 1404
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 2 cut(s) 625, 1236
BsmAI GTCTC 3 cut(s) 841, 1199, 1244
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 2 cut(s) 36, 127
BsnI GGCC 3 cut(s) 301, 917, 1166
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 395, 612, 701
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 2 cut(s) 1291, 1328
BspANI GGCC 3 cut(s) 301, 917, 1166
BspCNI CTCAG 7 cut(s) 88, 540, 726, 1012, 1108, 1195, 1294
BspLI GGNNCC 6 cut(s) 385, 397, 540, 829, 888, 1039
BspMAI CTGCAG 1 cut(s) 1006
BspMI ACCTGC 1 cut(s) 983
BspPI GGATC 3 cut(s) 390, 403, 709
BspT107I GGYRCC 2 cut(s) 538, 886
BsrBI CCGCTC 1 cut(s) 1330
BsrFI RCCGGY 2 cut(s) 16, 1082
BsrI ACTGG 3 cut(s) 361, 1108, 1265
BssAI RCCGGY 2 cut(s) 16, 1082
BssECI CCNNGG 5 cut(s) 378, 724, 725, 1194, 1363
BssMI GATC 3 cut(s) 395, 612, 701
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 378, 1194
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 5 cut(s) 168, 180, 388, 726, 1364
Bst4CI ACNGT 4 cut(s) 192, 259, 439, 988
Bst6I CTCTTC 6 cut(s) 73, 196, 650, 762, 1137, 1145
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstC8I GCNNGC 1 cut(s) 34
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 3 cut(s) 446, 636, 720
BstHHI GCGC 2 cut(s) 417, 1009
BstKTI GATC 3 cut(s) 398, 615, 704
BstMAI GTCTC 3 cut(s) 841, 1199, 1244
BstMBI GATC 3 cut(s) 395, 612, 701
BstNI CCWGG 5 cut(s) 168, 180, 388, 726, 1364
BstSCI CCNGG 6 cut(s) 166, 178, 386, 724, 1362, 1403
BstSFI CTRYAG 1 cut(s) 1002
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 8 cut(s) 16, 1016, 1036, 1037, 1058, 1078, 1082, 1187
BstX2I RGATCY 2 cut(s) 395, 701
BstYI RGATCY 2 cut(s) 395, 701
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 3 cut(s) 301, 917, 1166
BtsCI GGATG 3 cut(s) 446, 636, 720
BtsI GCAGTG 3 cut(s) 583, 1035, 1125
BtsIMutI CAGTG 6 cut(s) 188, 368, 444, 583, 1035, 1125
BveI ACCTGC 1 cut(s) 983
Cac8I GCNNGC 1 cut(s) 34
CaiI CAGNNNCTG 1 cut(s) 1363
CfoI GCGC 2 cut(s) 417, 1009
Cfr10I RCCGGY 2 cut(s) 16, 1082
Cfr13I GGNCC 4 cut(s) 384, 598, 827, 1191
Csp6I GTAC 4 cut(s) 605, 743, 880, 1360
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 8 cut(s) 209, 400, 824, 883, 913, 956, 1088, 1254
CviQI GTAC 4 cut(s) 605, 743, 880, 1360
DpnI GATC 3 cut(s) 397, 614, 703
DpnII GATC 3 cut(s) 395, 612, 701
DriI GACNNNNNGTC 1 cut(s) 833
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 6 cut(s) 73, 196, 650, 762, 1137, 1145
Eam1105I GACNNNNNGTC 1 cut(s) 833
EarI CTCTTC 6 cut(s) 73, 196, 650, 762, 1137, 1145
Eco130I CCWWGG 2 cut(s) 378, 1194
Eco32I GATATC 1 cut(s) 273
Eco47I GGWCC 4 cut(s) 384, 598, 827, 1191
Eco57I CTGAAG 1 cut(s) 1200
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 384
EcoRII CCWGG 5 cut(s) 166, 178, 386, 724, 1362
EcoRV GATATC 1 cut(s) 273
EcoT14I CCWWGG 2 cut(s) 378, 1194
ErhI CCWWGG 2 cut(s) 378, 1194
FaeI CATG 8 cut(s) 212, 403, 827, 886, 916, 959, 1091, 1257
FaqI GGGAC 1 cut(s) 454
FatI CATG 8 cut(s) 208, 399, 823, 882, 912, 955, 1087, 1253
FauNDI CATATG 1 cut(s) 1287
Fnu4HI GCNGC 8 cut(s) 30, 1005, 1026, 1047, 1050, 1071, 1092, 1176
FokI GGATG 3 cut(s) 433, 643, 707
Fsp4HI GCNGC 8 cut(s) 30, 1005, 1026, 1047, 1050, 1071, 1092, 1176
FspBI CTAG 3 cut(s) 107, 500, 1317
GlaI GCGC 2 cut(s) 416, 1008
GluI GCNGC 8 cut(s) 30, 1005, 1026, 1047, 1050, 1071, 1092, 1176
GsaI CCCAGC 2 cut(s) 1240, 1374
GsuI CTGGAG 1 cut(s) 260
HaeIII GGCC 3 cut(s) 301, 917, 1166
HapII CCGG 4 cut(s) 17, 1065, 1083, 1404
HhaI GCGC 2 cut(s) 417, 1009
Hin1II CATG 8 cut(s) 212, 403, 827, 886, 916, 959, 1091, 1257
Hin6I GCGC 2 cut(s) 415, 1007
HinP1I GCGC 2 cut(s) 415, 1007
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 4 cut(s) 86, 834, 1226, 1277
HpaII CCGG 4 cut(s) 17, 1065, 1083, 1404
HphI GGTGA 6 cut(s) 128, 271, 277, 396, 1111, 1118
Hpy166II GTNNAC 1 cut(s) 1125
Hpy188I TCNGA 8 cut(s) 85, 294, 549, 654, 668, 1134, 1204, 1272
Hpy188III TCNNGA 3 cut(s) 239, 393, 526
Hpy8I GTNNAC 1 cut(s) 1125
HpyAV CCTTC 3 cut(s) 256, 552, 1132
HpyCH4III ACNGT 4 cut(s) 192, 259, 439, 988
HpyCH4V TGCA 9 cut(s) 36, 133, 157, 860, 926, 943, 1004, 1016, 1052
Hsp92II CATG 8 cut(s) 212, 403, 827, 886, 916, 959, 1091, 1257
HspAI GCGC 2 cut(s) 415, 1007
Kzo9I GATC 3 cut(s) 395, 612, 701
LmnI GCTCC 4 cut(s) 1067, 1240, 1344, 1407
Lsp1109I GCAGC 8 cut(s) 16, 1016, 1036, 1037, 1058, 1078, 1082, 1187
MaeI CTAG 3 cut(s) 107, 500, 1317
MaeIII GTNAC 2 cut(s) 402, 1099
MalI GATC 3 cut(s) 397, 614, 703
MbiI CCGCTC 1 cut(s) 1330
MboI GATC 3 cut(s) 395, 612, 701
MflI RGATCY 2 cut(s) 395, 701
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 8 cut(s) 134, 160, 280, 482, 553, 669, 759, 1333
MlyI GAGTC 1 cut(s) 843
MmeI TCCRAC 2 cut(s) 632, 977
MroXI GAANNNNTTC 1 cut(s) 328
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 3 cut(s) 193, 213, 917
MspA1I CMGCKG 2 cut(s) 1049, 1178
MspI CCGG 4 cut(s) 17, 1065, 1083, 1404
MspR9I CCNGG 6 cut(s) 168, 180, 388, 726, 1364, 1405
Mva1269I GAATGC 2 cut(s) 36, 127
MvaI CCWGG 5 cut(s) 168, 180, 388, 726, 1364
NciI CCSGG 1 cut(s) 1405
NdeI CATATG 1 cut(s) 1287
NdeII GATC 3 cut(s) 395, 612, 701
NlaIII CATG 8 cut(s) 212, 403, 827, 886, 916, 959, 1091, 1257
NlaIV GGNNCC 6 cut(s) 385, 397, 540, 829, 888, 1039
NmuCI GTSAC 2 cut(s) 402, 1099
OliI CACNNNNGTG 1 cut(s) 193
PasI CCCWGGG 1 cut(s) 725
PctI GAATGC 2 cut(s) 36, 127
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 3 cut(s) 86, 1226, 1277
PflMI CCANNNNNTGG 1 cut(s) 1236
PkrI GCNGC 8 cut(s) 31, 1006, 1027, 1048, 1051, 1072, 1093, 1177
PleI GAGTC 1 cut(s) 842
PpsI GAGTC 1 cut(s) 842
PpuMI RGGWCCY 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 5 cut(s) 166, 178, 386, 724, 1362
PspFI CCCAGC 2 cut(s) 1236, 1370
PspGI CCWGG 5 cut(s) 166, 178, 386, 724, 1362
PspN4I GGNNCC 6 cut(s) 385, 397, 540, 829, 888, 1039
PspPI GGNCC 4 cut(s) 384, 598, 827, 1191
PspPPI RGGWCCY 1 cut(s) 384
PstI CTGCAG 1 cut(s) 1006
PstNI CAGNNNCTG 1 cut(s) 1363
PsuI RGATCY 2 cut(s) 395, 701
PvuII CAGCTG 2 cut(s) 1049, 1178
RsaI GTAC 4 cut(s) 606, 744, 881, 1361
RsaNI GTAC 4 cut(s) 605, 743, 880, 1360
RseI CAYNNNNRTG 3 cut(s) 193, 213, 917
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 8 cut(s) 30, 1005, 1026, 1047, 1050, 1071, 1092, 1176
Sau3AI GATC 3 cut(s) 395, 612, 701
Sau96I GGNCC 4 cut(s) 384, 598, 827, 1191
ScaI AGTACT 1 cut(s) 744
SchI GAGTC 1 cut(s) 843
ScrFI CCNGG 6 cut(s) 168, 180, 388, 726, 1364, 1405
SduI GDGCHC 1 cut(s) 353
SfcI CTRYAG 1 cut(s) 1002
SinI GGWCC 4 cut(s) 384, 598, 827, 1191
SmiMI CAYNNNNRTG 3 cut(s) 193, 213, 917
SmlI CTYRAG 1 cut(s) 1390
SmoI CTYRAG 1 cut(s) 1390
Sse9I AATT 8 cut(s) 134, 160, 280, 482, 553, 669, 759, 1333
SsiI CCGC 2 cut(s) 1291, 1328
SspMI CTAG 3 cut(s) 107, 500, 1317
StyD4I CCNGG 6 cut(s) 166, 178, 386, 724, 1362, 1403
StyI CCWWGG 2 cut(s) 378, 1194
TaaI ACNGT 4 cut(s) 192, 259, 439, 988
TaqI TCGA 1 cut(s) 284
TasI AATT 8 cut(s) 134, 160, 280, 482, 553, 669, 759, 1333
TatI WGTACW 3 cut(s) 604, 742, 879
TfiI GAWTC 3 cut(s) 86, 1226, 1277
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 6 cut(s) 195, 368, 444, 583, 1035, 1132
TseFI GTSAC 2 cut(s) 402, 1099
TseI GCWGC 8 cut(s) 29, 1004, 1025, 1046, 1049, 1070, 1091, 1175
Tsp45I GTSAC 2 cut(s) 402, 1099
TspDTI ATGAA 5 cut(s) 197, 435, 546, 647, 672
TspGWI ACGGA 1 cut(s) 448
TspRI CASTG 6 cut(s) 195, 368, 444, 583, 1035, 1132
Van91I CCANNNNNTGG 1 cut(s) 1236
VpaK11BI GGWCC 4 cut(s) 384, 598, 827, 1191
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 4 cut(s) 160, 280, 482, 669
XmnI GAANNNNTTC 1 cut(s) 328
XspI CTAG 3 cut(s) 107, 500, 1317
ZrmI AGTACT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.