Rmu_sc0002494.1_g000006

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002494.1
OG0002279
Physical Location & Seq
Forward (+)
47343 .. 51310
3968 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2088 bp
atggctccatctgcaagcacatcgaaagttgctagatctatagccttaaggataaaggcggtgcagatgaaacgtagtagaaaaaccgtatctgaaccacctattacttctccaaatcgtaaacctgctacccccaagaaggctgaagcaagattgacagaagccaacaaggctaccctccctaggagaagtccgcgatcgaataaagcaaaggaagctgctcagtctagcaaggatgcggctgccaagagaaatatgcgaccgcctaaagcagcaaagtccaagaaggctgtggcatcaaagaagtcgtcaactggtagtaagtctgcaaagagcaagtcatccgaggattctgatgatgatgagaatgtcactggtggattgaggttgcttaagcgagggatggctgccaagagaaatatgcgaccgcctaaagcagcaaagtccaagaaggctgtggcatcaaaaaagtcatcaactggtagtaagtctgcaaagagcaagtcatccgaggattctgatgatgatgagaatgtcactggtggattggggttgcttaagcgagggatggtaaccatgaatcgaattactaggcgactgcttcaaggaaagaagctaaaggtgatttctaatgctgatggggagccaattgggaaggcagctaaggagatgcagtcctatattggggtgttggcacgcaccaagatcccaatatctataaatgattggagaaatgtgcctttggatgaaaaagacaagatttggaatagtattcaggatgcttatgtggtgccaaaagagtggaagaaactggttattgcatcagctgccaacaaatggagagagttcaagagcaaactaactaactggtacattattccctatctagatactcctgaactcttacagttccctccagatgattatcgatccattgaggtagatgaatggaatacgttcgtggctgatagaacttcaaaacaatttcaggcactccgcgaaatgcagagaacaaagaggaaggagaataagtatcctcatcgaatgtcacggaaaggatatgctaacttccaggaagaactggaaaatttgaagaggaaagtgtctgaaggagaaatcaaccctgaaggaacagatgatgttctgactttggcgttgggaaatcctgagtgtcccggtagggtacgcggtgttggtggcttcgtcaagccttctgcattttttcatctacctaaacgacaaaagctaaatgtagaagatagtgtcagggcaagtgttaagaagatactagcagaggagaaagaaaatatcattgctgaggaaagagccaaatgggagttggagatggagcaacaaattgctagggaaagagcagcttgggaagaaaggattcagaagttagaagcgaagtttgatgggaaagagatgccagctgactcaaccaaacctgtgacaccgctgaatgatttagggtctggccagggcagctgttctaggcattttgacaaggctggtttgaaagttattgaaagtgaagccgccaaaattgccaagaagttagatttaggggaggaccagcagaaagtagttgaggaggatgaaatttttgtggatggggagctgaacatcaaactaacctcaattattgaggaggaggctccacttgatccaagcactgccttgggaggattctctggaaacaactattatgagtcttctgcatttgtagtcacctacccaattaataatgcagttgatgacgtaggaaatgagaacgggaaggcactggcttgggagaaggcttttattcagttagcaaagatctggagcaaatatgtttataaaaaaactgttgccaatggtgctgtctcggaactgagtctctcattttcagttgaaagctcaattgaggaagagttaataagagagtactgcagatgttataaccataatagtaagttatatggtaatgttcctgtatatatctctgacattgggagatgcacctcaaatctcttctttttacctctcatccaaggtccaaaggtgaagccaaagcaatgttttaaggcaaaaagggtttcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

695

Amino Acids

77.51

Weight (kDa)

9.61

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPC1_MLD PF22314 496 - 611 4.5e-12 NPC1, middle luminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1842, 1944
Acc36I ACCTGC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 790
AccB7I CCANNNNNTGG 1 cut(s) 837
AccII CGCG 3 cut(s) 196, 999, 1188
AciI CCGC 9 cut(s) 59, 194, 239, 263, 428, 997, 1188, 1457, 1539
AclWI GGATC 3 cut(s) 700, 924, 1661
AcoI YGGCCR 1 cut(s) 1477
AcsI RAATTY 2 cut(s) 1087, 1602
AcuI CTGAAG 3 cut(s) 165, 1128, 1146
AfaI GTAC 3 cut(s) 872, 1185, 1931
AfiI CCNNNNNNNGG 4 cut(s) 139, 183, 684, 837
AflII CTTAAG 3 cut(s) 46, 392, 557
AgsI TTSAA 7 cut(s) 605, 850, 978, 1093, 1519, 1529, 1898
AjnI CCWGG 2 cut(s) 1071, 1479
Alw26I GTCTC 2 cut(s) 1873, 1886
AlwI GGATC 3 cut(s) 700, 924, 1661
AoxI GGCC 1 cut(s) 1477
ApeKI GCWGC 9 cut(s) 218, 242, 272, 407, 437, 659, 827, 1373, 1485
ApoI RAATTY 2 cut(s) 1087, 1602
ArsI GACNNNNNNTTYG 6 cut(s) 293, 323, 325, 355, 488, 520
AseI ATTAAT 1 cut(s) 1743
Asp700I GAANNNNTTC 2 cut(s) 956, 1389
AspA2I CCTAGG 1 cut(s) 182
AspS9I GGNCC 2 cut(s) 1573, 2039
AsuC2I CCSGG 1 cut(s) 1176
AsuHPI GGTGA 3 cut(s) 634, 1723, 2059
AvaII GGWCC 2 cut(s) 1573, 2039
AvrII CCTAGG 1 cut(s) 182
BalI TGGCCA 1 cut(s) 1479
BanI GGYRCC 1 cut(s) 790
BbsI GAAGAC 1 cut(s) 1707
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 9 cut(s) 205, 229, 284, 394, 449, 671, 814, 1385, 1497
BccI CCATC 7 cut(s) 16, 397, 562, 632, 1339, 1409, 1607
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 2 cut(s) 1073, 1481
BciVI GTATCC 1 cut(s) 1044
BcnI CCSGG 1 cut(s) 1176
BcoDI GTCTC 2 cut(s) 1873, 1886
BfaI CTAG 8 cut(s) 33, 183, 228, 591, 887, 1289, 1362, 1494
BfmI CTRYAG 2 cut(s) 39, 1933
BfrI CTTAAG 3 cut(s) 46, 392, 557
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 1044
BglI GCCNNNNNGGC 1 cut(s) 170
BglII AGATCT 2 cut(s) 35, 1821
BlnI CCTAGG 1 cut(s) 182
BmcAI AGTACT 1 cut(s) 1931
Bme1390I CCNGG 3 cut(s) 1073, 1176, 1481
Bme18I GGWCC 2 cut(s) 1573, 2039
BmgT120I GGNCC 2 cut(s) 1573, 2039
BmiI GGNNCC 4 cut(s) 6, 645, 792, 1659
BmrFI CCNGG 3 cut(s) 1073, 1176, 1481
BmsI GCATC 8 cut(s) 226, 305, 470, 660, 769, 830, 1416, 1991
BpiI GAAGAC 1 cut(s) 1707
BpmI CTGGAG 2 cut(s) 900, 1846
Bpu10I CCTNAGC 2 cut(s) 663, 1317
BpuMI CCSGG 1 cut(s) 1176
Bsa29I ATCGAT 1 cut(s) 928
BsaBI GATNNNNATC 1 cut(s) 924
BsaJI CCNNGG 6 cut(s) 182, 345, 510, 1480, 1680, 2035
BsaXI ACNNNNNCTCC 4 cut(s) 659, 689, 1016, 1046
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 183, 684, 837
Bse1I ACTGG 8 cut(s) 319, 379, 484, 544, 816, 872, 1086, 1791
Bse3DI GCAATG 2 cut(s) 1311, 2066
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 2 cut(s) 1073, 1481
BseCI ATCGAT 1 cut(s) 928
BseDI CCNNGG 6 cut(s) 182, 345, 510, 1480, 1680, 2035
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 4 cut(s) 139, 183, 684, 837
BseMI GCAATG 2 cut(s) 1311, 2066
BseMII CTCAG 4 cut(s) 236, 1158, 1308, 1868
BseNI ACTGG 8 cut(s) 319, 379, 484, 544, 816, 872, 1086, 1791
BseRI GAGGAG 4 cut(s) 1310, 1607, 1664, 1667
BseXI GCAGC 9 cut(s) 205, 229, 284, 394, 449, 671, 814, 1385, 1497
BsgI GTGCAG 1 cut(s) 83
Bsh1236I CGCG 3 cut(s) 196, 999, 1188
Bsh1285I CGRYCG 3 cut(s) 200, 263, 428
BshFI GGCC 1 cut(s) 1479
BshNI GGYRCC 1 cut(s) 790
BshVI ATCGAT 1 cut(s) 928
BsiEI CGRYCG 3 cut(s) 200, 263, 428
BsiSI CCGG 1 cut(s) 1176
BslFI GGGAC 1 cut(s) 1158
BslI CCNNNNNNNGG 4 cut(s) 139, 183, 684, 837
BsmAI GTCTC 2 cut(s) 1873, 1886
BsmFI GGGAC 1 cut(s) 1158
BsnI GGCC 1 cut(s) 1479
Bsp143I GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
BspACI CCGC 9 cut(s) 59, 194, 239, 263, 428, 997, 1188, 1457, 1539
BspANI GGCC 1 cut(s) 1479
BspCNI CTCAG 4 cut(s) 235, 1159, 1309, 1869
BspDI ATCGAT 1 cut(s) 928
BspFNI CGCG 3 cut(s) 196, 999, 1188
BspLI GGNNCC 4 cut(s) 6, 645, 792, 1659
BspMAI CTGCAG 1 cut(s) 1937
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 3 cut(s) 700, 924, 1661
BspT107I GGYRCC 1 cut(s) 790
BspTI CTTAAG 3 cut(s) 46, 392, 557
BsrDI GCAATG 2 cut(s) 1311, 2066
BsrI ACTGG 8 cut(s) 319, 379, 484, 544, 816, 872, 1086, 1791
BssECI CCNNGG 6 cut(s) 182, 345, 510, 1480, 1680, 2035
BssMI GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
BssT1I CCWWGG 3 cut(s) 182, 1680, 2035
Bst2UI CCWGG 2 cut(s) 1073, 1481
Bst4CI ACNGT 3 cut(s) 88, 909, 1852
Bst6I CTCTTC 3 cut(s) 1088, 1908, 2021
BstAFI CTTAAG 3 cut(s) 46, 392, 557
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 3 cut(s) 16, 697, 1431
BstDEI CTNAG 6 cut(s) 222, 663, 1167, 1317, 1877, 2085
BstEII GGTNACC 1 cut(s) 571
BstFNI CGCG 3 cut(s) 196, 999, 1188
BstKTI GATC 6 cut(s) 38, 200, 708, 932, 1669, 1824
BstMAI GTCTC 2 cut(s) 1873, 1886
BstMBI GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
BstMCI CGRYCG 3 cut(s) 200, 263, 428
BstMWI GCNNNNNNNGC 7 cut(s) 11, 170, 215, 827, 1485, 1547, 1862
BstNI CCWGG 2 cut(s) 1073, 1481
BstPI GGTNACC 1 cut(s) 571
BstSCI CCNGG 3 cut(s) 1071, 1174, 1479
BstSFI CTRYAG 2 cut(s) 39, 1933
BstUI CGCG 3 cut(s) 196, 999, 1188
BstV1I GCAGC 9 cut(s) 205, 229, 284, 394, 449, 671, 814, 1385, 1497
BstV2I GAAGAC 1 cut(s) 1707
BstX2I RGATCY 3 cut(s) 35, 705, 1821
BstXI CCANNNNNNTGG 1 cut(s) 801
BstYI RGATCY 3 cut(s) 35, 705, 1821
Bsu15I ATCGAT 1 cut(s) 928
BsuI GTATCC 1 cut(s) 1044
BsuRI GGCC 1 cut(s) 1479
BsuTUI ATCGAT 1 cut(s) 928
BtsI GCAGTG 1 cut(s) 1674
BtsIMutI CAGTG 4 cut(s) 372, 537, 1674, 1784
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 3 cut(s) 16, 697, 1431
Cfr13I GGNCC 2 cut(s) 1573, 2039
ClaI ATCGAT 1 cut(s) 928
Csp6I GTAC 3 cut(s) 871, 1184, 1930
CviAII CATG 1 cut(s) 577
CviQI GTAC 3 cut(s) 871, 1184, 1930
DdeI CTNAG 6 cut(s) 222, 663, 1167, 1317, 1877, 2085
DpnI GATC 6 cut(s) 37, 199, 707, 931, 1668, 1823
DpnII GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
EaeI YGGCCR 1 cut(s) 1477
Eam1104I CTCTTC 3 cut(s) 1088, 1908, 2021
EarI CTCTTC 3 cut(s) 1088, 1908, 2021
Eco130I CCWWGG 3 cut(s) 182, 1680, 2035
Eco47I GGWCC 2 cut(s) 1573, 2039
Eco57I CTGAAG 3 cut(s) 165, 1128, 1146
Eco91I GGTNACC 1 cut(s) 571
EcoO65I GGTNACC 1 cut(s) 571
EcoRII CCWGG 2 cut(s) 1071, 1479
EcoT14I CCWWGG 3 cut(s) 182, 1680, 2035
ErhI CCWWGG 3 cut(s) 182, 1680, 2035
FaeI CATG 1 cut(s) 580
FalI AAGNNNNNCTT 3 cut(s) 1360, 1392, 2068
FaqI GGGAC 1 cut(s) 1158
FatI CATG 1 cut(s) 576
FspBI CTAG 8 cut(s) 33, 183, 228, 591, 887, 1289, 1362, 1494
GsuI CTGGAG 2 cut(s) 900, 1846
HaeIII GGCC 1 cut(s) 1479
HapII CCGG 1 cut(s) 1176
Hin1II CATG 1 cut(s) 580
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HinfI GANTC 8 cut(s) 350, 515, 580, 1390, 1436, 1689, 1712, 1879
HpaII CCGG 1 cut(s) 1176
HphI GGTGA 3 cut(s) 634, 1723, 2059
Hpy166II GTNNAC 2 cut(s) 122, 312
Hpy188III TCNNGA 8 cut(s) 776, 850, 887, 896, 917, 1166, 1695, 1825
Hpy8I GTNNAC 2 cut(s) 122, 312
HpyCH4III ACNGT 3 cut(s) 88, 909, 1852
HpyCH4IV ACGT 3 cut(s) 73, 956, 1761
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 170, 215, 827, 1485, 1547, 1862
HpyF3I CTNAG 6 cut(s) 222, 663, 1167, 1317, 1877, 2085
HpySE526I ACGT 3 cut(s) 73, 956, 1761
Hsp92II CATG 1 cut(s) 580
Kzo9I GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
LmnI GCTCC 6 cut(s) 10, 643, 1348, 1618, 1663, 1827
Lsp1109I GCAGC 9 cut(s) 205, 229, 284, 394, 449, 671, 814, 1385, 1497
LweI GCATC 8 cut(s) 226, 305, 470, 660, 769, 830, 1416, 1991
MaeI CTAG 8 cut(s) 33, 183, 228, 591, 887, 1289, 1362, 1494
MaeII ACGT 3 cut(s) 73, 956, 1761
MaeIII GTNAC 6 cut(s) 370, 535, 571, 1047, 1450, 1729
MalI GATC 6 cut(s) 37, 199, 707, 931, 1668, 1823
MboI GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
MboII GAAGA 9 cut(s) 817, 1088, 1105, 1268, 1294, 1394, 1707, 1925, 2008
MfeI CAATTG 2 cut(s) 648, 1905
MflI RGATCY 3 cut(s) 35, 705, 1821
MlsI TGGCCA 1 cut(s) 1479
MluNI TGGCCA 1 cut(s) 1479
MlyI GAGTC 3 cut(s) 1430, 1721, 1888
MmeI TCCRAC 1 cut(s) 1320
Mox20I TGGCCA 1 cut(s) 1479
MroXI GAANNNNTTC 2 cut(s) 956, 1389
MscI TGGCCA 1 cut(s) 1479
MseI TTAA 7 cut(s) 47, 393, 558, 1278, 1743, 1919, 2067
Msp20I TGGCCA 1 cut(s) 1479
MspA1I CMGCKG 4 cut(s) 827, 1433, 1459, 1488
MspCI CTTAAG 3 cut(s) 46, 392, 557
MspI CCGG 1 cut(s) 1176
MspR9I CCNGG 3 cut(s) 1073, 1176, 1481
MunI CAATTG 2 cut(s) 648, 1905
MvaI CCWGG 2 cut(s) 1073, 1481
MvnI CGCG 3 cut(s) 196, 999, 1188
MwoI GCNNNNNNNGC 7 cut(s) 11, 170, 215, 827, 1485, 1547, 1862
NciI CCSGG 1 cut(s) 1176
NdeII GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
NlaIII CATG 1 cut(s) 580
NlaIV GGNNCC 4 cut(s) 6, 645, 792, 1659
NmuCI GTSAC 5 cut(s) 370, 535, 1047, 1450, 1729
PdmI GAANNNNTTC 2 cut(s) 956, 1389
PfeI GAWTC 5 cut(s) 350, 515, 580, 1390, 1689
PflMI CCANNNNNTGG 1 cut(s) 837
PfoI TCCNGGA 1 cut(s) 1071
Ple19I CGATCG 1 cut(s) 200
PleI GAGTC 3 cut(s) 1430, 1720, 1887
PpsI GAGTC 3 cut(s) 1430, 1720, 1887
PshBI ATTAAT 1 cut(s) 1743
PsiI TTATAA 2 cut(s) 1842, 1944
Psp6I CCWGG 2 cut(s) 1071, 1479
PspEI GGTNACC 1 cut(s) 571
PspGI CCWGG 2 cut(s) 1071, 1479
PspN4I GGNNCC 4 cut(s) 6, 645, 792, 1659
PspPI GGNCC 2 cut(s) 1573, 2039
PstI CTGCAG 1 cut(s) 1937
PsuI RGATCY 3 cut(s) 35, 705, 1821
PvuI CGATCG 1 cut(s) 200
PvuII CAGCTG 3 cut(s) 827, 1433, 1488
RsaI GTAC 3 cut(s) 872, 1185, 1931
RsaNI GTAC 3 cut(s) 871, 1184, 1930
SaqAI TTAA 7 cut(s) 47, 393, 558, 1278, 1743, 1919, 2067
Sau3AI GATC 6 cut(s) 35, 197, 705, 929, 1666, 1821
Sau96I GGNCC 2 cut(s) 1573, 2039
ScaI AGTACT 1 cut(s) 1931
SchI GAGTC 3 cut(s) 1430, 1721, 1888
ScrFI CCNGG 3 cut(s) 1073, 1176, 1481
SfaNI GCATC 8 cut(s) 226, 305, 470, 660, 769, 830, 1416, 1991
SfcI CTRYAG 2 cut(s) 39, 1933
SinI GGWCC 2 cut(s) 1573, 2039
SmlI CTYRAG 3 cut(s) 46, 392, 557
SmoI CTYRAG 3 cut(s) 46, 392, 557
SsiI CCGC 9 cut(s) 59, 194, 239, 263, 428, 997, 1188, 1457, 1539
SspMI CTAG 8 cut(s) 33, 183, 228, 591, 887, 1289, 1362, 1494
StyD4I CCNGG 3 cut(s) 1071, 1174, 1479
StyI CCWWGG 3 cut(s) 182, 1680, 2035
TaaI ACNGT 3 cut(s) 88, 909, 1852
TaiI ACGT 3 cut(s) 76, 959, 1764
TaqI TCGA 5 cut(s) 23, 200, 583, 928, 1042
TatI WGTACW 1 cut(s) 1929
TauI GCSGC 2 cut(s) 242, 1541
TfiI GAWTC 5 cut(s) 350, 515, 580, 1390, 1689
Tru1I TTAA 7 cut(s) 47, 393, 558, 1278, 1743, 1919, 2067
Tru9I TTAA 7 cut(s) 47, 393, 558, 1278, 1743, 1919, 2067
TscAI CASTG 4 cut(s) 379, 544, 1681, 1791
TseFI GTSAC 5 cut(s) 370, 535, 1047, 1450, 1729
TseI GCWGC 9 cut(s) 218, 242, 272, 407, 437, 659, 827, 1373, 1485
Tsp45I GTSAC 5 cut(s) 370, 535, 1047, 1450, 1729
TspDTI ATGAA 6 cut(s) 83, 593, 762, 960, 1214, 1614
TspGWI ACGGA 1 cut(s) 1066
TspRI CASTG 4 cut(s) 379, 544, 1681, 1791
Van91I CCANNNNNTGG 1 cut(s) 837
Vha464I CTTAAG 3 cut(s) 46, 392, 557
VpaK11BI GGWCC 2 cut(s) 1573, 2039
VspI ATTAAT 1 cut(s) 1743
XapI RAATTY 2 cut(s) 1087, 1602
XbaI TCTAGA 1 cut(s) 886
XcmI CCANNNNNNNNNTGG 3 cut(s) 289, 454, 1336
XmaJI CCTAGG 1 cut(s) 182
XmnI GAANNNNTTC 2 cut(s) 956, 1389
XspI CTAG 8 cut(s) 33, 183, 228, 591, 887, 1289, 1362, 1494
ZrmI AGTACT 1 cut(s) 1931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.