Rroxscaffold_4G00312410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002279
Physical Location & Seq
Forward (+)
35379317 .. 35403841
24525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312410.1

Sequence Viewer

Length: 543 bp
ATGTCGCCACCTTCTCCGGCAAGTCGCTTAAGAGCTTTGATGACAGAGATGGACTCGGATTGCAGATCATTGTCGGTGCAGTCTCTCTCGCAGGGCCGCCTTCTCCTCCCGCAAGGATTTCCCGATAACCGCGACTTCTTCGTCGAATTCGCCCTCATTCTCCGGCCACACTATCCGATCGAGGCAAAAGAAAAATGCATGAGCCGGATCGGCCAGAGCATGCTCGACGGCTCTGACACCGTTTTAAAGGTCGCCTTCGAGTTGGCGCGAGGCGCGGAGTCGCCGAATTTGCTCTCCAACCTTGACAATTCAATGAACGAAGACCGGAGATGGGACAACGACACCGAGAAGAAGACATTGGCGCCGGTACGACGGCCGAGAGATGGCTGGACGGCGAGGTTCCGGCCGGAGCACTTGAAAGCTTTGTGTATCTGCTGGAGAAAAAGAAAGAAACTGCAAAAGAAGAAACCGATTGAATTAAATATACAAGGACTAAATCTTACAAATAAGGACAATGTGCTCTCCACTTACCACATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

20.68

Weight (kDa)

9.54

Isoelectric Point (pI)

77.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 361
AccII CGCG 3 cut(s) 132, 268, 275
AciI CCGC 4 cut(s) 97, 110, 130, 275
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 4 cut(s) 164, 211, 374, 404
AcsI RAATTY 2 cut(s) 146, 286
AcyI GRCGYC 1 cut(s) 362
AfaI GTAC 1 cut(s) 369
AfiI CCNNNNNNNGG 2 cut(s) 331, 383
AflII CTTAAG 1 cut(s) 28
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 3 cut(s) 312, 418, 476
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AluBI AGCT 2 cut(s) 35, 422
AluI AGCT 2 cut(s) 35, 422
Alw21I GWGCWC 2 cut(s) 414, 522
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 215
AoxI GGCC 5 cut(s) 94, 164, 211, 374, 404
ApoI RAATTY 2 cut(s) 146, 286
AspLEI GCGC 3 cut(s) 268, 275, 364
AspS9I GGNCC 1 cut(s) 94
BanI GGYRCC 1 cut(s) 361
BbsI GAAGAC 2 cut(s) 327, 359
Bbv12I GWGCWC 2 cut(s) 414, 522
BccI CCATC 3 cut(s) 43, 324, 377
BceAI ACGGC 3 cut(s) 244, 389, 408
BcoDI GTCTC 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 365
BfrI CTTAAG 1 cut(s) 28
BglI GCCNNNNNGGC 1 cut(s) 210
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 363, 401
BpiI GAAGAC 2 cut(s) 327, 359
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpmI CTGGAG 1 cut(s) 457
BsaHI GRCGYC 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 324
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 383
Bse118I RCCGGY 1 cut(s) 364
BseLI CCNNNNNNNGG 2 cut(s) 331, 383
BseRI GAGGAG 1 cut(s) 95
BseX3I CGGCCG 2 cut(s) 374, 404
BsgI GTGCAG 1 cut(s) 98
Bsh1236I CGCG 3 cut(s) 132, 268, 275
Bsh1285I CGRYCG 3 cut(s) 180, 377, 407
BshFI GGCC 5 cut(s) 96, 166, 213, 376, 406
BshNI GGYRCC 1 cut(s) 361
BsiEI CGRYCG 3 cut(s) 180, 377, 407
BsiHKAI GWGCWC 2 cut(s) 414, 522
BsiSI CCGG 7 cut(s) 17, 163, 205, 325, 365, 403, 407
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 2 cut(s) 331, 383
BsmAI GTCTC 1 cut(s) 87
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 5 cut(s) 96, 166, 213, 376, 406
Bsp1286I GDGCHC 2 cut(s) 414, 522
Bsp143I GATC 3 cut(s) 65, 177, 207
BspACI CCGC 4 cut(s) 97, 110, 130, 275
BspANI GGCC 5 cut(s) 96, 166, 213, 376, 406
BspFNI CGCG 3 cut(s) 132, 268, 275
BspHI TCATGA 1 cut(s) 539
BspLI GGNNCC 2 cut(s) 363, 401
BspPI GGATC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 361
BspTI CTTAAG 1 cut(s) 28
BsrFI RCCGGY 1 cut(s) 364
BssAI RCCGGY 1 cut(s) 364
BssMI GATC 3 cut(s) 65, 177, 207
BssNI GRCGYC 1 cut(s) 362
Bst4CI ACNGT 1 cut(s) 241
BstACI GRCGYC 1 cut(s) 362
BstAFI CTTAAG 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 221
BstFNI CGCG 3 cut(s) 132, 268, 275
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 3 cut(s) 268, 275, 364
BstKTI GATC 3 cut(s) 68, 180, 210
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 3 cut(s) 65, 177, 207
BstMCI CGRYCG 3 cut(s) 180, 377, 407
BstMWI GCNNNNNNNGC 3 cut(s) 210, 272, 289
BstNSI RCATGY 2 cut(s) 223, 538
BstUI CGCG 3 cut(s) 132, 268, 275
BstV2I GAAGAC 2 cut(s) 327, 359
BstZI CGGCCG 2 cut(s) 374, 404
BsuRI GGCC 5 cut(s) 96, 166, 213, 376, 406
Cac8I GCNNGC 1 cut(s) 221
CciI TCATGA 1 cut(s) 539
CfoI GCGC 3 cut(s) 268, 275, 364
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 368
CviAII CATG 4 cut(s) 199, 220, 535, 540
CviQI GTAC 1 cut(s) 368
DinI GGCGCC 1 cut(s) 363
DpnI GATC 3 cut(s) 67, 179, 209
DpnII GATC 3 cut(s) 65, 177, 207
DraI TTTAAA 1 cut(s) 246
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 4 cut(s) 164, 211, 374, 404
EagI CGGCCG 2 cut(s) 374, 404
EclXI CGGCCG 2 cut(s) 374, 404
Eco52I CGGCCG 2 cut(s) 374, 404
EcoRI GAATTC 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 200
EgeI GGCGCC 1 cut(s) 363
EheI GGCGCC 1 cut(s) 363
FaeI CATG 4 cut(s) 202, 223, 538, 543
FaiI YATR 5 cut(s) 200, 221, 485, 536, 541
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 1 cut(s) 347
FatI CATG 4 cut(s) 198, 219, 534, 539
FauI CCCGC 1 cut(s) 117
Fnu4HI GCNGC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 97
GlaI GCGC 3 cut(s) 267, 274, 363
GluI GCNGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 457
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 5 cut(s) 96, 166, 213, 376, 406
HapII CCGG 7 cut(s) 17, 163, 205, 325, 365, 403, 407
HhaI GCGC 3 cut(s) 268, 275, 364
Hin1I GRCGYC 1 cut(s) 362
Hin1II CATG 4 cut(s) 202, 223, 538, 543
Hin6I GCGC 3 cut(s) 266, 273, 362
HinP1I GCGC 3 cut(s) 266, 273, 362
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 2 cut(s) 53, 278
HpaII CCGG 7 cut(s) 17, 163, 205, 325, 365, 403, 407
Hpy188I TCNGA 3 cut(s) 58, 177, 235
Hpy188III TCNNGA 2 cut(s) 122, 540
Hpy99I CGWCG 3 cut(s) 146, 230, 375
HpyAV CCTTC 3 cut(s) 21, 110, 265
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 4 cut(s) 63, 79, 198, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 272, 289
Hsp92I GRCGYC 1 cut(s) 362
Hsp92II CATG 4 cut(s) 202, 223, 538, 543
HspAI GCGC 3 cut(s) 266, 273, 362
KasI GGCGCC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 65, 177, 207
LmnI GCTCC 1 cut(s) 409
MalI GATC 3 cut(s) 67, 179, 209
MboI GATC 3 cut(s) 65, 177, 207
MboII GAAGA 5 cut(s) 130, 332, 361, 364, 475
MhlI GDGCHC 2 cut(s) 414, 522
MluCI AATT 4 cut(s) 146, 286, 307, 476
Mly113I GGCGCC 1 cut(s) 362
MlyI GAGTC 2 cut(s) 47, 287
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 5 cut(s) 116, 164, 175, 263, 390
Mph1103I ATGCAT 1 cut(s) 200
MseI TTAA 3 cut(s) 29, 245, 479
MspCI CTTAAG 1 cut(s) 28
MspI CCGG 7 cut(s) 17, 163, 205, 325, 365, 403, 407
MvnI CGCG 3 cut(s) 132, 268, 275
MwoI GCNNNNNNNGC 3 cut(s) 210, 272, 289
NarI GGCGCC 1 cut(s) 362
NdeII GATC 3 cut(s) 65, 177, 207
NlaIII CATG 4 cut(s) 202, 223, 538, 543
NlaIV GGNNCC 2 cut(s) 363, 401
NmeAIII GCCGAG 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 200
NspI RCATGY 2 cut(s) 223, 538
PaeI GCATGC 1 cut(s) 223
PagI TCATGA 1 cut(s) 539
PciI ACATGT 1 cut(s) 534
PkrI GCNGC 1 cut(s) 98
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 2 cut(s) 47, 286
PluTI GGCGCC 1 cut(s) 365
PpsI GAGTC 2 cut(s) 47, 286
PscI ACATGT 1 cut(s) 534
PspN4I GGNNCC 2 cut(s) 363, 401
PspPI GGNCC 1 cut(s) 94
PvuI CGATCG 1 cut(s) 180
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 29, 245, 479
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 3 cut(s) 65, 177, 207
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 2 cut(s) 47, 287
SduI GDGCHC 2 cut(s) 414, 522
SetI ASST 6 cut(s) 13, 37, 252, 303, 401, 424
SfoI GGCGCC 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
SphI GCATGC 1 cut(s) 223
Sse9I AATT 4 cut(s) 146, 286, 307, 476
SsiI CCGC 4 cut(s) 97, 110, 130, 275
SspDI GGCGCC 1 cut(s) 361
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 4 cut(s) 144, 180, 225, 258
TasI AATT 4 cut(s) 146, 286, 307, 476
TauI GCSGC 1 cut(s) 99
Tru1I TTAA 3 cut(s) 29, 245, 479
Tru9I TTAA 3 cut(s) 29, 245, 479
TspDTI ATGAA 1 cut(s) 329
Vha464I CTTAAG 1 cut(s) 28
XapI RAATTY 2 cut(s) 146, 286
XceI RCATGY 2 cut(s) 223, 538
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.