Rmu_sc0003275.1_g000003

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003275.1
OG0002685
Physical Location & Seq
Reverse (-)
10315 .. 10845
531 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 429 bp
atgaagggtgcaacagcggcagaatatgtagttggtgacagcttgggctggactcttccccccaacacctccttctattcagattgggctgcctctaagacatttcagttgggcgatgtagtttcattcaattggactggaaatcatactatggcagaagcaactaaggaagaatatgacaactgcagtgacccgggaatctggattactactccggtgaatgtgaatcttatttctgctggttctcgctacttcatctgcaccgttggcgaccactgtgagcaaggccagaaggtgaccatcactgtaggatcatcctctccaacatctgctccaccaccgccttcaggcgcccatactactgttgcatctttactacttgggctcatcagctccacactagtcatttctttcttcacttgcatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

142

Amino Acids

15.03

Weight (kDa)

4.66

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 17 - 97 2.7e-18 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 350
AciI CCGC 2 cut(s) 17, 341
AclWI GGATC 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 330
AcyI GRCGYC 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 347
AgsI TTSAA 1 cut(s) 130
AhlI ACTAGT 1 cut(s) 400
AjuI GAANNNNNNNTTGG 4 cut(s) 15, 47, 56, 88
AluBI AGCT 2 cut(s) 42, 393
AluI AGCT 2 cut(s) 42, 393
AlwI GGATC 1 cut(s) 319
Ama87I CYCGRG 1 cut(s) 193
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 1 cut(s) 89
AspLEI GCGC 1 cut(s) 353
AsuC2I CCSGG 2 cut(s) 194, 195
AsuHPI GGTGA 3 cut(s) 47, 229, 307
AvaI CYCGRG 1 cut(s) 193
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 76
BccI CCATC 1 cut(s) 308
BcnI CCSGG 2 cut(s) 194, 195
BcuI ACTAGT 1 cut(s) 400
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 2 cut(s) 184, 306
BfoI RGCGCY 1 cut(s) 354
BisI GCNGC 2 cut(s) 18, 90
BlsI GCNGC 2 cut(s) 19, 91
Bme1390I CCNGG 2 cut(s) 194, 195
BmeT110I CYCGRG 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 2 cut(s) 194, 195
BmsI GCATC 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 194, 195
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 193
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 347
BseNI ACTGG 1 cut(s) 142
BseXI GCAGC 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 350
BsiHKCI CYCGRG 1 cut(s) 193
BsiSI CCGG 2 cut(s) 194, 215
BslI CCNNNNNNNGG 1 cut(s) 347
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 1 cut(s) 311
BspACI CCGC 2 cut(s) 17, 341
BspANI GGCC 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 352
BspMAI CTGCAG 1 cut(s) 188
BspPI GGATC 1 cut(s) 319
BspT107I GGYRCC 1 cut(s) 350
BsrI ACTGG 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 1 cut(s) 311
BssNI GRCGYC 1 cut(s) 351
Bst4CI ACNGT 4 cut(s) 265, 278, 307, 364
Bst6I CTCTTC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 96, 165
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 1 cut(s) 314
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 1 cut(s) 314
BstMBI GATC 1 cut(s) 311
BstMWI GCNNNNNNNGC 2 cut(s) 17, 267
BstNSI RCATGY 1 cut(s) 427
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 192, 193
BstSFI CTRYAG 2 cut(s) 184, 306
BstV1I GCAGC 1 cut(s) 76
BsuRI GGCC 1 cut(s) 288
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 314
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 3 cut(s) 193, 274, 303
CfoI GCGC 1 cut(s) 353
Cfr9I CCCGGG 1 cut(s) 193
CviAII CATG 1 cut(s) 424
CviJI RGCY 6 cut(s) 42, 48, 89, 288, 385, 393
CviKI_1 RGCY 6 cut(s) 42, 48, 89, 288, 385, 393
DdeI CTNAG 2 cut(s) 96, 165
DinI GGCGCC 1 cut(s) 352
DpnI GATC 1 cut(s) 313
DpnII GATC 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 387
Eco57I CTGAAG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 193
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 387
EgeI GGCGCC 1 cut(s) 352
EheI GGCGCC 1 cut(s) 352
FaeI CATG 1 cut(s) 427
FaiI YATR 6 cut(s) 27, 147, 152, 177, 357, 425
FatI CATG 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 18, 90
FokI GGATG 1 cut(s) 301
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 18, 90
FspBI CTAG 1 cut(s) 401
GlaI GCGC 1 cut(s) 352
GluI GCNGC 2 cut(s) 18, 90
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 1 cut(s) 288
HapII CCGG 2 cut(s) 194, 215
HhaI GCGC 1 cut(s) 353
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 1 cut(s) 427
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HinfI GANTC 3 cut(s) 52, 198, 226
HpaII CCGG 2 cut(s) 194, 215
HphI GGTGA 3 cut(s) 47, 229, 307
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 202
HpyAV CCTTC 3 cut(s) 82, 286, 354
HpyCH4III ACNGT 4 cut(s) 265, 278, 307, 364
HpyCH4V TGCA 5 cut(s) 11, 186, 261, 368, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 267
HpyF3I CTNAG 2 cut(s) 96, 165
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 1 cut(s) 427
HspAI GCGC 1 cut(s) 351
KasI GGCGCC 1 cut(s) 350
Kzo9I GATC 1 cut(s) 311
LmnI GCTCC 2 cut(s) 337, 398
LpnPI CCDG 8 cut(s) 34, 123, 187, 207, 225, 228, 302, 333
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 1 cut(s) 377
MaeI CTAG 1 cut(s) 401
MaeIII GTNAC 3 cut(s) 35, 188, 295
MalI GATC 1 cut(s) 313
MboI GATC 1 cut(s) 311
MboII GAAGA 3 cut(s) 47, 182, 406
MfeI CAATTG 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 1 cut(s) 130
Mly113I GGCGCC 1 cut(s) 351
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 347
MnlI CCTC 3 cut(s) 79, 103, 328
MslI CAYNNNNRTG 1 cut(s) 422
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 194, 215
MspR9I CCNGG 2 cut(s) 194, 195
MunI CAATTG 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 17, 267
NarI GGCGCC 1 cut(s) 351
NciI CCSGG 2 cut(s) 194, 195
NdeII GATC 1 cut(s) 311
NlaIII CATG 1 cut(s) 427
NlaIV GGNNCC 1 cut(s) 352
NmuCI GTSAC 3 cut(s) 35, 188, 295
NspI RCATGY 1 cut(s) 427
PfeI GAWTC 2 cut(s) 198, 226
PkrI GCNGC 2 cut(s) 19, 91
PleI GAGTC 1 cut(s) 46
PluTI GGCGCC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 46
PspEI GGTNACC 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 352
PstI CTGCAG 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 422
SatI GCNGC 2 cut(s) 18, 90
Sau3AI GATC 1 cut(s) 311
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 194, 195
SduI GDGCHC 1 cut(s) 387
SetI ASST 4 cut(s) 44, 71, 297, 395
SfaNI GCATC 1 cut(s) 377
SfcI CTRYAG 2 cut(s) 184, 306
SfoI GGCGCC 1 cut(s) 352
SmaI CCCGGG 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 422
SpeI ACTAGT 1 cut(s) 400
Sse9I AATT 1 cut(s) 130
SsiI CCGC 2 cut(s) 17, 341
SspDI GGCGCC 1 cut(s) 350
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 192, 193
TaaI ACNGT 4 cut(s) 265, 278, 307, 364
TasI AATT 1 cut(s) 130
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 198, 226
TscAI CASTG 3 cut(s) 193, 281, 310
TseFI GTSAC 3 cut(s) 35, 188, 295
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 3 cut(s) 35, 188, 295
TspDTI ATGAA 3 cut(s) 17, 114, 244
TspMI CCCGGG 1 cut(s) 193
TspRI CASTG 3 cut(s) 193, 281, 310
XceI RCATGY 1 cut(s) 427
XmaI CCCGGG 1 cut(s) 193
XspI CTAG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.