Rmu_sc0003573.1_g000043

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003573.1
OG0003968
Physical Location & Seq
Forward (+)
214169 .. 215531
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
atgagtgtgaaggttataatgaagatgatgatgacaattaatgtacatgatagagtgaatatagatgaagatggaagtaattacgtggatagtgattatgagatattgcccggggatgaaggatatggtgaagatggacaagatgacttggaatatgaggagaatgttgataattgtgatgtatatgacgagtatgctaacatgggattcaatggtgaaatttctgaccatagtcatgatagtgaagaattgagggatttggtggaagaaattgaaagtgatgatgaagaaggtgttaaagtagaggttgcatgcggaaagaagcacaagggtaaaagggagacttggtcttgtcaattttggatatttgctagtgtaatcaagggtaaggaaccagaattgattatcaagtcaatcaatttgaatcacaagaattgtgaaacggtttcaagagtgaagttttgtacttctacatttgtagcaaaagagtatgtacaaatgtggaaagctgatccatttatgtctagaaaactcttccaagatatagtatctcaagattttagtcaaaccatttcacacaagcaggttataagagcaaggaggctagctgcacttatgaattctgggaatgaggcagaccaatataatctcttggaaacctatgttgaggtattaagacaatcaaacctatggtcaattattgaattggtaaccgatatggatggaggtgttaggaagtttaaaagattttatgtatgttttgatgcatgcaagaggggttggatggctggttgtagatttgttgtggggttggatggctgccatataaagacaaagtaccctggtcagttgctctcagccatgggaattgatgctaacaatgagatgtttcctatcgcttatgatgtggttgagatggagaattacgaaacttggtgttggttcctagagtacttgattggagattttgaaatgcaaaatgatttcagttatgtatttatgagtgataaacagaaagggctactagatgcggttaatgacttaatgtcaaacagtgaacatagacactgtgtaaggcatctacacaacaatttcaaagttaagaatcctgaaattgtgtttaagcatctactttggtcaactgctagggatactacaaagacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

388

Amino Acids

45.02

Weight (kDa)

4.9

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MULE PF10551 268 - 364 6.3e-16 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 17, 590
Acc36I ACCTGC 1 cut(s) 574
AciI CCGC 2 cut(s) 315, 1031
AclWI GGATC 1 cut(s) 506
AcsI RAATTY 2 cut(s) 219, 619
AfaI GTAC 5 cut(s) 45, 466, 495, 839, 953
AgsI TTSAA 7 cut(s) 211, 275, 424, 450, 704, 971, 1096
AhdI GACNNNNNGTC 1 cut(s) 1045
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 2 cut(s) 509, 608
AluI AGCT 2 cut(s) 509, 608
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 608, 819
ApoI RAATTY 2 cut(s) 219, 619
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AseI ATTAAT 1 cut(s) 39
AsuC2I CCSGG 2 cut(s) 111, 112
AsuHPI GGTGA 2 cut(s) 140, 227
AsuNHI GCTAGC 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 110
BbvI GCAGC 2 cut(s) 595, 806
BccI CCATC 6 cut(s) 65, 128, 716, 778, 809, 910
BciT130I CCWGG 1 cut(s) 843
BciVI GTATCC 1 cut(s) 1144
BcnI CCSGG 2 cut(s) 111, 112
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 6 cut(s) 372, 525, 605, 947, 1025, 1146
BfuAI ACCTGC 1 cut(s) 574
BfuI GTATCC 1 cut(s) 1144
BisI GCNGC 2 cut(s) 609, 820
BlsI GCNGC 2 cut(s) 610, 821
BmcAI AGTACT 1 cut(s) 953
Bme1390I CCNGG 3 cut(s) 111, 112, 843
BmeRI GACNNNNNGTC 1 cut(s) 1045
BmeT110I CYCGRG 1 cut(s) 110
BmiI GGNNCC 2 cut(s) 393, 944
BmrFI CCNGG 3 cut(s) 111, 112, 843
BmsI GCATC 5 cut(s) 754, 862, 1018, 1087, 1135
BmtI GCTAGC 1 cut(s) 608
BoxI GACNNNNGTC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 537
BpuMI CCSGG 2 cut(s) 111, 112
BsaAI YACGTR 1 cut(s) 85
BsaJI CCNNGG 4 cut(s) 110, 111, 841, 861
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 4 cut(s) 110, 111, 841, 861
BseGI GGATG 4 cut(s) 121, 727, 789, 820
BseMII CTCAG 1 cut(s) 870
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 2 cut(s) 595, 806
BsgI GTGCAG 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 110
Bsp1407I TGTACA 2 cut(s) 43, 493
Bsp143I GATC 1 cut(s) 511
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 2 cut(s) 315, 1031
BspCNI CTCAG 1 cut(s) 869
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 393, 944
BspMI ACCTGC 1 cut(s) 574
BspOI GCTAGC 1 cut(s) 608
BspPI GGATC 1 cut(s) 506
BsrGI TGTACA 2 cut(s) 43, 493
BssECI CCNNGG 4 cut(s) 110, 111, 841, 861
BssMI GATC 1 cut(s) 511
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 445, 1055, 1070
Bst6I CTCTTC 1 cut(s) 539
BstAUI TGTACA 2 cut(s) 43, 493
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 313, 606, 769
BstDEI CTNAG 1 cut(s) 856
BstDSI CCRYGG 1 cut(s) 861
BstEII GGTNACC 1 cut(s) 709
BstF5I GGATG 4 cut(s) 121, 727, 789, 820
BstKTI GATC 1 cut(s) 514
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 1 cut(s) 511
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 2 cut(s) 315, 771
BstPAI GACNNNNGTC 1 cut(s) 231
BstPI GGTNACC 1 cut(s) 709
BstSCI CCNGG 3 cut(s) 109, 110, 841
BstV1I GCAGC 2 cut(s) 595, 806
BsuI GTATCC 1 cut(s) 1144
BtgI CCRYGG 1 cut(s) 861
BtsCI GGATG 4 cut(s) 121, 727, 789, 820
BtsIMutI CAGTG 2 cut(s) 1060, 1066
BveI ACCTGC 1 cut(s) 574
Cac8I GCNNGC 3 cut(s) 313, 606, 769
CciI TCATGA 1 cut(s) 235
Cfr9I CCCGGG 1 cut(s) 110
Csp6I GTAC 5 cut(s) 44, 465, 494, 838, 952
CviAII CATG 7 cut(s) 47, 202, 236, 312, 768, 862, 1164
CviJI RGCY 7 cut(s) 509, 604, 608, 788, 819, 860, 1021
CviKI_1 RGCY 7 cut(s) 509, 604, 608, 788, 819, 860, 1021
CviQI GTAC 5 cut(s) 44, 465, 494, 838, 952
DdeI CTNAG 1 cut(s) 856
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
DraI TTTAAA 1 cut(s) 742
DriI GACNNNNNGTC 1 cut(s) 1045
Eam1104I CTCTTC 1 cut(s) 539
Eam1105I GACNNNNNGTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 539
Eco130I CCWWGG 1 cut(s) 861
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 709
EcoO65I GGTNACC 1 cut(s) 709
EcoRI GAATTC 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 861
EcoT22I ATGCAT 1 cut(s) 769
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 7 cut(s) 50, 205, 239, 315, 771, 865, 1167
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FatI CATG 7 cut(s) 46, 201, 235, 311, 767, 861, 1163
Fnu4HI GCNGC 2 cut(s) 609, 820
FokI GGATG 4 cut(s) 128, 734, 796, 827
Fsp4HI GCNGC 2 cut(s) 609, 820
FspBI CTAG 6 cut(s) 372, 525, 605, 947, 1025, 1146
GluI GCNGC 2 cut(s) 609, 820
HapII CCGG 1 cut(s) 111
Hin1II CATG 7 cut(s) 50, 205, 239, 315, 771, 865, 1167
HincII GTYRAC 1 cut(s) 1140
HindII GTYRAC 1 cut(s) 1140
HinfI GANTC 3 cut(s) 207, 424, 1105
HpaII CCGG 1 cut(s) 111
HphI GGTGA 2 cut(s) 140, 227
Hpy166II GTNNAC 2 cut(s) 1058, 1140
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 5 cut(s) 236, 450, 525, 554, 1109
Hpy8I GTNNAC 2 cut(s) 1058, 1140
HpyAV CCTTC 3 cut(s) 4, 113, 284
HpyCH4III ACNGT 3 cut(s) 445, 1055, 1070
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 5 cut(s) 311, 611, 767, 771, 976
HpyF3I CTNAG 1 cut(s) 856
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 7 cut(s) 50, 205, 239, 315, 771, 865, 1167
Kzo9I GATC 1 cut(s) 511
LpnPI CCDG 8 cut(s) 124, 408, 569, 609, 774, 828, 855, 1122
Lsp1109I GCAGC 2 cut(s) 595, 806
LweI GCATC 5 cut(s) 754, 862, 1018, 1087, 1135
MaeI CTAG 6 cut(s) 372, 525, 605, 947, 1025, 1146
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 709
MalI GATC 1 cut(s) 513
MboI GATC 1 cut(s) 511
MboII GAAGA 7 cut(s) 34, 80, 143, 257, 278, 299, 526
MmeI TCCRAC 2 cut(s) 761, 792
MnlI CCTC 8 cut(s) 151, 246, 298, 594, 625, 661, 719, 768
Mph1103I ATGCAT 1 cut(s) 769
MseI TTAA 8 cut(s) 39, 297, 674, 741, 1035, 1043, 1101, 1122
MslI CAYNNNNRTG 2 cut(s) 234, 240
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 3 cut(s) 111, 112, 843
MvaI CCWGG 1 cut(s) 843
NciI CCSGG 2 cut(s) 111, 112
NcoI CCATGG 1 cut(s) 861
NdeII GATC 1 cut(s) 511
NheI GCTAGC 1 cut(s) 604
NlaIII CATG 7 cut(s) 50, 205, 239, 315, 771, 865, 1167
NlaIV GGNNCC 2 cut(s) 393, 944
NsiI ATGCAT 1 cut(s) 769
NspI RCATGY 2 cut(s) 315, 771
PaeI GCATGC 2 cut(s) 315, 771
PagI TCATGA 1 cut(s) 235
PfeI GAWTC 3 cut(s) 207, 424, 1105
PflFI GACNNNGTC 1 cut(s) 346
PkrI GCNGC 2 cut(s) 610, 821
Ppu21I YACGTR 1 cut(s) 85
PshAI GACNNNNGTC 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 39
PsiI TTATAA 2 cut(s) 17, 590
Psp6I CCWGG 1 cut(s) 841
PspEI GGTNACC 1 cut(s) 709
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 2 cut(s) 393, 944
PsyI GACNNNGTC 1 cut(s) 346
RsaI GTAC 5 cut(s) 45, 466, 495, 839, 953
RsaNI GTAC 5 cut(s) 44, 465, 494, 838, 952
RseI CAYNNNNRTG 2 cut(s) 234, 240
SaqAI TTAA 8 cut(s) 39, 297, 674, 741, 1035, 1043, 1101, 1122
SatI GCNGC 2 cut(s) 609, 820
Sau3AI GATC 1 cut(s) 511
ScaI AGTACT 1 cut(s) 953
ScrFI CCNGG 3 cut(s) 111, 112, 843
SfaNI GCATC 5 cut(s) 754, 862, 1018, 1087, 1135
SmaI CCCGGG 1 cut(s) 112
SmiMI CAYNNNNRTG 2 cut(s) 234, 240
SmlI CTYRAG 1 cut(s) 552
SmoI CTYRAG 1 cut(s) 552
SphI GCATGC 2 cut(s) 315, 771
SsiI CCGC 2 cut(s) 315, 1031
SspMI CTAG 6 cut(s) 372, 525, 605, 947, 1025, 1146
StyD4I CCNGG 3 cut(s) 109, 110, 841
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 3 cut(s) 445, 1055, 1070
TaiI ACGT 1 cut(s) 87
TatI WGTACW 4 cut(s) 43, 464, 493, 951
TfiI GAWTC 3 cut(s) 207, 424, 1105
Tru1I TTAA 8 cut(s) 39, 297, 674, 741, 1035, 1043, 1101, 1122
Tru9I TTAA 8 cut(s) 39, 297, 674, 741, 1035, 1043, 1101, 1122
TscAI CASTG 2 cut(s) 1060, 1073
TseI GCWGC 2 cut(s) 608, 819
TspDTI ATGAA 5 cut(s) 35, 81, 132, 300, 632
TspMI CCCGGG 1 cut(s) 110
TspRI CASTG 2 cut(s) 1060, 1073
Tth111I GACNNNGTC 1 cut(s) 346
VspI ATTAAT 1 cut(s) 39
XapI RAATTY 2 cut(s) 219, 619
XbaI TCTAGA 1 cut(s) 524
XceI RCATGY 2 cut(s) 315, 771
XmaI CCCGGG 1 cut(s) 110
XspI CTAG 6 cut(s) 372, 525, 605, 947, 1025, 1146
ZrmI AGTACT 1 cut(s) 953
Zsp2I ATGCAT 1 cut(s) 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.