Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0003624.1

Orthogroup ID

OG0003132

Physical Location & Seq

Genomic Coordinates Forward (+)

56149 .. 59321

Gene Structure 3173 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

atggacgttgatgaaccaaccaaaggcgaagaagaaaagcaaggaaagcccataatcgtagtattgatgggtgcacccggtagcggcaagtccacattctgcgaacaagtcatgggctcctccgttcgtccctgggtccgggtgtgccaggataccatcaagaatgggaaagccggtacgaaagctcagtgtatagagagcgcgaggagtgcattgagggaaggaaagagcgtgtttatagatagatgcaatctggagaaagagcaaagagatgagtttgtgaagctgggtggtcctcaggtggatgttcatgctgtggtgcttgatcttcctgctaagctttgtatctcgcggtcggtgaagcgaactggccatgaggggaacttgcaaggtggaaaagctgcggctgttgtgaatagaatgctgcagaagaaagaactgccgaatctgagcgaagggtttggtaggatcactttttgccagaatgagagtgatgttgaatctgccattcgtacatatagtggactcagtccgttggatacactttcccatggtacttttggccagaagagcccgggtgcaaaagttcaacttggtataatgaaattcctaaagaaaacagatgctcctactaatactgaatcgacttcaaacaaggttcaggattctaatgcttctcaaattactgggggaaaggataccagtgtgaaaggaacaggtttgtttactgaaagtggtagtatggagtccaagaaaggtgagcagcctgtcgtaggttctgctggcactgacttctctcttgacaatgctcccaccctggcatttccatctatttccacagcagattttcagtttgaccttgagaaggcatctgacattattgtagagaaagttgcagaatttgtaaataaacttggaaatgcaagacttgttttagtggacttgactcataaatcgaagattttgtccttggtgagggctaaagcttcacagaaaaacattgactccaagaggttcttcacatttgtgggagatatcactaagcttcattcagaaggaggtctttgctgcaatgtgatagctaatgctgctaactggcgacttaaaccaggaggtggtggtgtcaatgctgcaatcttcaatgctggaggtccagctttagaggttgcaaccaaagaacaagccaaatctctgtatcctgggaatgctgtggttgttcctctcccttcaacttctcctttgttttgtagggaaggggtaacccatgtcatacatgttcttggacccaatatgaacccacagagaccaaattatcttgacaatgactatagcaaaggctgcaaaattctccatgacacttatacttcactttttgaaggttttgcatcaatagtgaggacccaaaagaaggtatttaagggaagctttggaaaccttcggttgaagctgccagagtcaaaagatcaccctgagagtgggcctacaaatcactctacaaatacctatcaaaagatcaagagagaggatcttcatgaatctgaaaggaacaaacggagtaaaggagatcaagctgaagctgaaaacgtctctgcttctaatgctagtaaatcaaacctccactctgatggaagcaagaataaatcctggggaacatgggcccaggctgtctacaacattgccatgcatccagagaagcagagggatgtagtactagagatatcagatgatgtcgttgtactaaatgatctttatccaaaggcacaaaagcatctcctggtggtagcacgacatcctggtcttgatcgcttggcagatgtatgcaaagaacacattcagttgctaaggacaatgcatgcggttggtttgaagtgggctgaaaagttcttacatgatgattcaacattggtctttcgtcttggatatcactcggaaccatcaatgcgacagctgcacctacatgtaatcagccaggattttaattcagcacatttgaagaacaagaaacattggaattcctttaatactgctttctttcgagactctgtggatgtgctagaggaagtcagtagtgacggaaaggcaatactgaatgatgatgaaagcctgatgtctatggagttacgatgtaatcgatgtagaagtgctcaccccaccattcccaaattgaaattgcacattggcagatgtcgagcctccttcccaagcaccctgcttgaaaatggtcggctggtaactgcaccaagcaattctagcaatgatccatag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0016787 GO:0016817 GO:0016819 GO:0019637 GO:0019693 GO:0034641 GO:0044237 GO:0044238 GO:0044281 GO:0046483 GO:0047627 GO:0055086 GO:0071704 GO:0072521 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_33 DcpS_C HLH Macro zf-C2HE

Protein Analysis

754

Amino Acids

82.52

Weight (kDa)

8.87

Isoelectric Point (pI)

33.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_33	PF13671	20 - 146	6.8e-13	AAA domain
Macro	PF01661	362 - 443	1.2e-06	Macro domain
DcpS_C	PF11969	555 - 669	5.1e-25	Scavenger mRNA decapping enzyme C-term binding
HIT	PF01230	565 - 658	6.7e-09	HIT domain
zf-C2HE	PF16278	674 - 726	1.3e-08	C2HE / C2H2 / C2HC zinc-binding finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01310
fragaria_vesca	FvH4_6g11090 FvH4_6g11090 FvH4_6g11090 FvH4_6g11090 FvH4_6g11090
malus_domestica	MD04G1160300.v1.1 MD05G1095500.v1.1 MD10G1110900.v1.1 MD12G1172800.v1.1
prunus_persica	Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1
pyrus_communis	pycom04g14360 pycom12g16400 pycom12g16440
rosa_chinensis	RchiOBHm_Chr3g0462501 RchiOBHm_Chr3g0462521
rosa_laevigata	RLG00000024853 RLG00000024857
rosa_multiflora	Rmu_sc0003624.1_g000009 Rmu_sc0003624.1_g000012 Rmu_sc0003624.1_g000015 Rmu_sc0011346.1_g000002 Rmu_sc0011346.1_g000005
rosa_roxburghii	Rroxscaffold_6G00417460
rosa_rugosa	Rorug03G0057000
rosa_samantha	Rh3AG115200 Rh3BG118500 Rh3BG118600 Rh3CG121400 Rh3CG122000 Rh3DG120700
rosa_wichuraiana	Rw0G003810 Rw3G009750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1794
AccB7I	CCANNNNNTGG	1 cut(s)	1115
AccI	GTMKAC	1 cut(s)	1668
AccII	CGCG	2 cut(s)	203, 352
AciI	CCGC	4 cut(s)	84, 352, 404, 1856
AclWI	GGATC	3 cut(s)	476, 1533, 2252
AcoI	YGGCCR	2 cut(s)	370, 562
AcsI	RAATTY	4 cut(s)	605, 899, 1344, 2011
AcuI	CTGAAG	1 cut(s)	1593
AfaI	GTAC	5 cut(s)	178, 514, 556, 1710, 1737
AfiI	CCNNNNNNNGG	9 cut(s)	23, 83, 138, 773, 865, 975, 1115, 1408, 1475
AflIII	ACRYGT	2 cut(s)	1273, 1957
AjuI	GAANNNNNNNTTGG	2 cut(s)	1001, 1033
AleI	CACNNNNGTG	1 cut(s)	1025
Alw21I	GWGCWC	2 cut(s)	76, 2146
Alw26I	GTCTC	3 cut(s)	1297, 1591, 2031
Alw44I	GTGCAC	1 cut(s)	72
AlwI	GGATC	3 cut(s)	476, 1533, 2252
Ama87I	CYCGRG	1 cut(s)	574
AoxI	GGCC	4 cut(s)	370, 562, 1478, 1656
ApaI	GGGCCC	1 cut(s)	1660
ApaLI	GTGCAC	1 cut(s)	72
ApeKI	GCWGC	9 cut(s)	401, 424, 763, 1068, 1088, 1130, 1338, 1447, 1948
ApoI	RAATTY	4 cut(s)	605, 899, 1344, 2011
ArsI	GACNNNNNNTTYG	2 cut(s)	950, 982
Asp700I	GAANNNNTTC	1 cut(s)	1830
AspLEI	GCGC	1 cut(s)	203
AspS9I	GGNCC	8 cut(s)	136, 293, 1151, 1283, 1398, 1478, 1656, 1657
AsuC2I	CCSGG	4 cut(s)	78, 140, 575, 576
AsuHPI	GGTGA	5 cut(s)	370, 770, 985, 1457, 2138
AvaI	CYCGRG	1 cut(s)	574
AvaII	GGWCC	5 cut(s)	136, 293, 1151, 1283, 1398
AxyI	CCTNAGG	1 cut(s)	297
BaeGI	GKGCMC	2 cut(s)	76, 1660
BaeI	ACNNNNGTAYC	2 cut(s)	690, 723
BalI	TGGCCA	2 cut(s)	372, 564
BanII	GRGCYC	3 cut(s)	119, 575, 1660
Bbv12I	GWGCWC	2 cut(s)	76, 2146
BbvI	GCAGC	9 cut(s)	388, 411, 775, 1055, 1075, 1117, 1325, 1434, 1935
BccI	CCATC	5 cut(s)	61, 164, 835, 1619, 1942
BciVI	GTATCC	4 cut(s)	145, 532, 691, 1206
BcnI	CCSGG	4 cut(s)	78, 140, 575, 576
BcoDI	GTCTC	3 cut(s)	1297, 1591, 2031
BfaI	CTAG	4 cut(s)	1602, 1712, 2054, 2250
BfmI	CTRYAG	2 cut(s)	425, 1327
BfuI	GTATCC	4 cut(s)	145, 532, 691, 1206
BlpI	GCTNAGC	1 cut(s)	336
BmcAI	AGTACT	1 cut(s)	1710
Bme18I	GGWCC	5 cut(s)	136, 293, 1151, 1283, 1398
BmeT110I	CYCGRG	1 cut(s)	574
BmgT120I	GGNCC	8 cut(s)	136, 293, 1151, 1283, 1398, 1478, 1656, 1657
BmiI	GGNNCC	6 cut(s)	118, 137, 1285, 1400, 1658, 1932
BmrI	ACTGGG	1 cut(s)	696
BmsI	GCATC	6 cut(s)	236, 613, 878, 1394, 1693, 1777
BmuI	ACTGGG	1 cut(s)	696
BpmI	CTGGAG	2 cut(s)	275, 1167
Bpu10I	CCTNAGC	1 cut(s)	1841
Bpu1102I	GCTNAGC	1 cut(s)	336
BpuEI	CTTGAG	1 cut(s)	881
BpuMI	CCSGG	4 cut(s)	78, 140, 575, 576
Bsa29I	ATCGAT	1 cut(s)	2131
BsaBI	GATNNNNATC	1 cut(s)	1749
BsaI	GGTCTC	1 cut(s)	1297
BsaJI	CCNNGG	9 cut(s)	131, 132, 550, 574, 816, 969, 1199, 1644, 1659
BsaXI	ACNNNNNCTCC	4 cut(s)	610, 640, 989, 1019
Bsc4I	CCNNNNNNNGG	9 cut(s)	23, 83, 138, 773, 865, 975, 1115, 1408, 1475
Bse118I	RCCGGY	1 cut(s)	173
Bse1I	ACTGG	4 cut(s)	373, 691, 702, 1100
Bse21I	CCTNAGG	1 cut(s)	297
Bse3DI	GCAATG	3 cut(s)	1078, 1674, 2260
Bse8I	GATNNNNATC	1 cut(s)	1749
BseCI	ATCGAT	1 cut(s)	2131
BseDI	CCNNGG	9 cut(s)	131, 132, 550, 574, 816, 969, 1199, 1644, 1659
BseGI	GGATG	5 cut(s)	310, 1684, 1708, 1789, 2053
BseJI	GATNNNNATC	1 cut(s)	1749
BseLI	CCNNNNNNNGG	9 cut(s)	23, 83, 138, 773, 865, 975, 1115, 1408, 1475
BseMI	GCAATG	3 cut(s)	1078, 1674, 2260
BseMII	CTCAG	5 cut(s)	200, 311, 440, 541, 1461
BseNI	ACTGG	4 cut(s)	373, 691, 702, 1100
BseRI	GAGGAG	2 cut(s)	109, 220
BseSI	GKGCMC	2 cut(s)	76, 1660
BseXI	GCAGC	9 cut(s)	388, 411, 775, 1055, 1075, 1117, 1325, 1434, 1935
BseYI	CCCAGC	1 cut(s)	286
BsgI	GTGCAG	2 cut(s)	1934, 2220
Bsh1236I	CGCG	2 cut(s)	203, 352
Bsh1285I	CGRYCG	1 cut(s)	356
BshFI	GGCC	4 cut(s)	372, 564, 1480, 1658
BshVI	ATCGAT	1 cut(s)	2131
BsiEI	CGRYCG	1 cut(s)	356
BsiHKAI	GWGCWC	2 cut(s)	76, 2146
BsiHKCI	CYCGRG	1 cut(s)	574
BsiSI	CCGG	4 cut(s)	78, 139, 174, 575
BslFI	GGGAC	1 cut(s)	114
BslI	CCNNNNNNNGG	9 cut(s)	23, 83, 138, 773, 865, 975, 1115, 1408, 1475
BsmAI	GTCTC	3 cut(s)	1297, 1591, 2031
BsmBI	CGTCTC	1 cut(s)	1591
BsmFI	GGGAC	1 cut(s)	114
BsmI	GAATGC	2 cut(s)	426, 1210
BsnI	GGCC	4 cut(s)	372, 564, 1480, 1658
Bso31I	GGTCTC	1 cut(s)	1297
BsoBI	CYCGRG	1 cut(s)	574
Bsp120I	GGGCCC	1 cut(s)	1656
Bsp1286I	GDGCHC	5 cut(s)	76, 119, 575, 1660, 2146
Bsp143I	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
Bsp1720I	GCTNAGC	1 cut(s)	336
Bsp19I	CCATGG	1 cut(s)	550
BspACI	CCGC	4 cut(s)	84, 352, 404, 1856
BspANI	GGCC	4 cut(s)	372, 564, 1480, 1658
BspCNI	CTCAG	5 cut(s)	199, 310, 441, 540, 1462
BspDI	ATCGAT	1 cut(s)	2131
BspFNI	CGCG	2 cut(s)	203, 352
BspHI	TCATGA	1 cut(s)	1531
BspLI	GGNNCC	6 cut(s)	118, 137, 1285, 1400, 1658, 1932
BspMAI	CTGCAG	1 cut(s)	429
BspPI	GGATC	3 cut(s)	476, 1533, 2252
BspQI	GCTCTTC	1 cut(s)	563
BspTNI	GGTCTC	1 cut(s)	1297
BsrDI	GCAATG	3 cut(s)	1078, 1674, 2260
BsrFI	RCCGGY	1 cut(s)	173
BsrI	ACTGG	4 cut(s)	373, 691, 702, 1100
BssAI	RCCGGY	1 cut(s)	173
BssECI	CCNNGG	9 cut(s)	131, 132, 550, 574, 816, 969, 1199, 1644, 1659
BssMI	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
BssT1I	CCWWGG	2 cut(s)	550, 969
Bst6I	CTCTTC	1 cut(s)	563
BstAPI	GCANNNNNTGC	1 cut(s)	1338
BstC8I	GCNNGC	2 cut(s)	784, 1854
BstDEI	CTNAG	8 cut(s)	186, 297, 336, 449, 527, 1041, 1470, 1841
BstDSI	CCRYGG	1 cut(s)	550
BstEII	GGTNACC	1 cut(s)	1258
BstENI	CCTNNNNNAGG	3 cut(s)	771, 863, 973
BstF5I	GGATG	5 cut(s)	310, 1684, 1708, 1789, 2053
BstFNI	CGCG	2 cut(s)	203, 352
BstHHI	GCGC	1 cut(s)	203
BstKTI	GATC	9 cut(s)	328, 471, 1465, 1515, 1528, 1567, 1747, 1804, 2260
BstMAI	GTCTC	3 cut(s)	1297, 1591, 2031
BstMBI	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
BstMCI	CGRYCG	1 cut(s)	356
BstMWI	GCNNNNNNNGC	8 cut(s)	46, 209, 570, 1088, 1338, 1598, 1948, 2250
BstNSI	RCATGY	3 cut(s)	1277, 1856, 1961
BstPI	GGTNACC	1 cut(s)	1258
BstSFI	CTRYAG	2 cut(s)	425, 1327
BstSLI	GKGCMC	2 cut(s)	76, 1660
BstUI	CGCG	2 cut(s)	203, 352
BstV1I	GCAGC	9 cut(s)	388, 411, 775, 1055, 1075, 1117, 1325, 1434, 1935
BstX2I	RGATCY	1 cut(s)	1525
BstXI	CCANNNNNNTGG	1 cut(s)	1625
BstYI	RGATCY	1 cut(s)	1525
Bsu15I	ATCGAT	1 cut(s)	2131
Bsu36I	CCTNAGG	1 cut(s)	297
BsuI	GTATCC	4 cut(s)	145, 532, 691, 1206
BsuRI	GGCC	4 cut(s)	372, 564, 1480, 1658
BsuTUI	ATCGAT	1 cut(s)	2131
BtgI	CCRYGG	1 cut(s)	550
BtsCI	GGATG	5 cut(s)	310, 1684, 1708, 1789, 2053
BtsIMutI	CAGTG	3 cut(s)	194, 709, 786
Cac8I	GCNNGC	2 cut(s)	784, 1854
CciI	TCATGA	1 cut(s)	1531
CfoI	GCGC	1 cut(s)	203
Cfr10I	RCCGGY	1 cut(s)	173
Cfr13I	GGNCC	8 cut(s)	136, 293, 1151, 1283, 1398, 1478, 1656, 1657
Cfr9I	CCCGGG	1 cut(s)	574
ClaI	ATCGAT	1 cut(s)	2131
Csp6I	GTAC	5 cut(s)	177, 513, 555, 1709, 1736
CviQI	GTAC	5 cut(s)	177, 513, 555, 1709, 1736
DdeI	CTNAG	8 cut(s)	186, 297, 336, 449, 527, 1041, 1470, 1841
DpnI	GATC	9 cut(s)	327, 470, 1464, 1514, 1527, 1566, 1746, 1803, 2259
DpnII	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
DrdI	GACNNNNNNGTC	1 cut(s)	1794
DseDI	GACNNNNNNGTC	1 cut(s)	1794
EaeI	YGGCCR	2 cut(s)	370, 562
Eam1104I	CTCTTC	1 cut(s)	563
EarI	CTCTTC	1 cut(s)	563
Eco130I	CCWWGG	2 cut(s)	550, 969
Eco24I	GRGCYC	3 cut(s)	119, 575, 1660
Eco31I	GGTCTC	1 cut(s)	1297
Eco32I	GATATC	3 cut(s)	1036, 1719, 1922
Eco47I	GGWCC	5 cut(s)	136, 293, 1151, 1283, 1398
Eco57I	CTGAAG	1 cut(s)	1593
Eco81I	CCTNAGG	1 cut(s)	297
Eco88I	CYCGRG	1 cut(s)	574
Eco91I	GGTNACC	1 cut(s)	1258
EcoNI	CCTNNNNNAGG	3 cut(s)	771, 863, 973
EcoO109I	RGGNCCY	1 cut(s)	1398
EcoO65I	GGTNACC	1 cut(s)	1258
EcoRI	GAATTC	1 cut(s)	2011
EcoRV	GATATC	3 cut(s)	1036, 1719, 1922
EcoT14I	CCWWGG	2 cut(s)	550, 969
EcoT22I	ATGCAT	2 cut(s)	1686, 1854
EcoT38I	GRGCYC	3 cut(s)	119, 575, 1660
ErhI	CCWWGG	2 cut(s)	550, 969
Esp3I	CGTCTC	1 cut(s)	1591
FalI	AAGNNNNNCTT	8 cut(s)	576, 608, 1001, 1033, 1047, 1079, 1409, 1441
FaqI	GGGAC	1 cut(s)	114
FblI	GTMKAC	1 cut(s)	1668
FokI	GGATG	5 cut(s)	317, 1671, 1715, 1776, 2060
FriOI	GRGCYC	3 cut(s)	119, 575, 1660
FspBI	CTAG	4 cut(s)	1602, 1712, 2054, 2250
GlaI	GCGC	1 cut(s)	202
GsaI	CCCAGC	1 cut(s)	290
GsuI	CTGGAG	2 cut(s)	275, 1167
HaeIII	GGCC	4 cut(s)	372, 564, 1480, 1658
HapII	CCGG	4 cut(s)	78, 139, 174, 575
HhaI	GCGC	1 cut(s)	203
Hin6I	GCGC	1 cut(s)	201
HinP1I	GCGC	1 cut(s)	201
HindIII	AAGCTT	4 cut(s)	338, 984, 1043, 1423
HpaII	CCGG	4 cut(s)	78, 139, 174, 575
HphI	GGTGA	5 cut(s)	370, 770, 985, 1457, 2138
Hpy166II	GTNNAC	6 cut(s)	74, 93, 524, 726, 940, 1669
Hpy188I	TCNGA	7 cut(s)	450, 874, 1054, 1540, 1624, 1723, 1930
Hpy8I	GTNNAC	6 cut(s)	74, 93, 524, 726, 940, 1669
HpyCH4IV	ACGT	2 cut(s)	6, 1584
HpyF10VI	GCNNNNNNNGC	8 cut(s)	46, 209, 570, 1088, 1338, 1598, 1948, 2250
HpyF3I	CTNAG	8 cut(s)	186, 297, 336, 449, 527, 1041, 1470, 1841
HpySE526I	ACGT	2 cut(s)	6, 1584
HspAI	GCGC	1 cut(s)	201
Kzo9I	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
LguI	GCTCTTC	1 cut(s)	563
LmnI	GCTCC	3 cut(s)	122, 631, 814
Lsp1109I	GCAGC	9 cut(s)	388, 411, 775, 1055, 1075, 1117, 1325, 1434, 1935
LweI	GCATC	6 cut(s)	236, 613, 878, 1394, 1693, 1777
MaeI	CTAG	4 cut(s)	1602, 1712, 2054, 2250
MaeII	ACGT	2 cut(s)	6, 1584
MaeIII	GTNAC	4 cut(s)	1258, 2069, 2118, 2230
MalI	GATC	9 cut(s)	327, 470, 1464, 1514, 1527, 1566, 1746, 1803, 2259
MboI	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
MflI	RGATCY	1 cut(s)	1525
MhlI	GDGCHC	5 cut(s)	76, 119, 575, 1660, 2146
MlsI	TGGCCA	2 cut(s)	372, 564
MluNI	TGGCCA	2 cut(s)	372, 564
MlyI	GAGTC	6 cut(s)	519, 755, 940, 998, 1463, 2033
MmeI	TCCRAC	1 cut(s)	516
Mox20I	TGGCCA	2 cut(s)	372, 564
Mph1103I	ATGCAT	2 cut(s)	1686, 1854
MroXI	GAANNNNTTC	1 cut(s)	1830
MscI	TGGCCA	2 cut(s)	372, 564
MseI	TTAA	4 cut(s)	1104, 1416, 1977, 2019
MslI	CAYNNNNRTG	4 cut(s)	1025, 1623, 1679, 1956
Msp20I	TGGCCA	2 cut(s)	372, 564
MspA1I	CMGCKG	1 cut(s)	1948
MspI	CCGG	4 cut(s)	78, 139, 174, 575
Mva1269I	GAATGC	2 cut(s)	426, 1210
MvnI	CGCG	2 cut(s)	203, 352
MwoI	GCNNNNNNNGC	8 cut(s)	46, 209, 570, 1088, 1338, 1598, 1948, 2250
NciI	CCSGG	4 cut(s)	78, 140, 575, 576
NcoI	CCATGG	1 cut(s)	550
NdeII	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
NlaIV	GGNNCC	6 cut(s)	118, 137, 1285, 1400, 1658, 1932
NmuCI	GTSAC	1 cut(s)	2069
NsiI	ATGCAT	2 cut(s)	1686, 1854
NspI	RCATGY	3 cut(s)	1277, 1856, 1961
OliI	CACNNNNGTG	1 cut(s)	1025
PaeI	GCATGC	1 cut(s)	1856
PagI	TCATGA	1 cut(s)	1531
PasI	CCCWGGG	1 cut(s)	132
PciI	ACATGT	2 cut(s)	1273, 1957
PciSI	GCTCTTC	1 cut(s)	563
PcsI	WCGNNNNNNNCGW	1 cut(s)	2128
PctI	GAATGC	2 cut(s)	426, 1210
PdmI	GAANNNNTTC	1 cut(s)	1830
PfeI	GAWTC	6 cut(s)	445, 500, 641, 665, 1535, 1895
PflFI	GACNNNGTC	1 cut(s)	528
PflMI	CCANNNNNTGG	1 cut(s)	1115
PleI	GAGTC	6 cut(s)	519, 754, 940, 998, 1462, 2033
PpsI	GAGTC	6 cut(s)	519, 754, 940, 998, 1462, 2033
PpuMI	RGGWCCY	1 cut(s)	1398
PscI	ACATGT	2 cut(s)	1273, 1957
Psp5II	RGGWCCY	1 cut(s)	1398
PspEI	GGTNACC	1 cut(s)	1258
PspFI	CCCAGC	1 cut(s)	286
PspN4I	GGNNCC	6 cut(s)	118, 137, 1285, 1400, 1658, 1932
PspOMI	GGGCCC	1 cut(s)	1656
PspPI	GGNCC	8 cut(s)	136, 293, 1151, 1283, 1398, 1478, 1656, 1657
PspPPI	RGGWCCY	1 cut(s)	1398
PstI	CTGCAG	1 cut(s)	429
PsuI	RGATCY	1 cut(s)	1525
PsyI	GACNNNGTC	1 cut(s)	528
PvuII	CAGCTG	1 cut(s)	1948
RsaI	GTAC	5 cut(s)	178, 514, 556, 1710, 1737
RsaNI	GTAC	5 cut(s)	177, 513, 555, 1709, 1736
RseI	CAYNNNNRTG	4 cut(s)	1025, 1623, 1679, 1956
SapI	GCTCTTC	1 cut(s)	563
SaqAI	TTAA	4 cut(s)	1104, 1416, 1977, 2019
Sau3AI	GATC	9 cut(s)	325, 468, 1462, 1512, 1525, 1564, 1744, 1801, 2257
Sau96I	GGNCC	8 cut(s)	136, 293, 1151, 1283, 1398, 1478, 1656, 1657
ScaI	AGTACT	1 cut(s)	1710
SchI	GAGTC	6 cut(s)	519, 755, 940, 998, 1463, 2033
SduI	GDGCHC	5 cut(s)	76, 119, 575, 1660, 2146
SfaNI	GCATC	6 cut(s)	236, 613, 878, 1394, 1693, 1777
SfcI	CTRYAG	2 cut(s)	425, 1327
SinI	GGWCC	5 cut(s)	136, 293, 1151, 1283, 1398
SmaI	CCCGGG	1 cut(s)	576
SmiMI	CAYNNNNRTG	4 cut(s)	1025, 1623, 1679, 1956
SmlI	CTYRAG	1 cut(s)	860
SmoI	CTYRAG	1 cut(s)	860
SphI	GCATGC	1 cut(s)	1856
SsiI	CCGC	4 cut(s)	84, 352, 404, 1856
SspMI	CTAG	4 cut(s)	1602, 1712, 2054, 2250
StyI	CCWWGG	2 cut(s)	550, 969
TaiI	ACGT	2 cut(s)	9, 1587
TaqI	TCGA	5 cut(s)	644, 956, 2035, 2131, 2188
TatI	WGTACW	2 cut(s)	1708, 1735
TauI	GCSGC	2 cut(s)	87, 407
TfiI	GAWTC	6 cut(s)	445, 500, 641, 665, 1535, 1895
Tru1I	TTAA	4 cut(s)	1104, 1416, 1977, 2019
Tru9I	TTAA	4 cut(s)	1104, 1416, 1977, 2019
TscAI	CASTG	3 cut(s)	194, 709, 793
TseFI	GTSAC	1 cut(s)	2069
TseI	GCWGC	9 cut(s)	401, 424, 763, 1068, 1088, 1130, 1338, 1447, 1948
Tsp45I	GTSAC	1 cut(s)	2069
TspDTI	ATGAA	8 cut(s)	27, 299, 617, 1037, 1307, 1520, 1548, 2112
TspGWI	ACGGA	4 cut(s)	112, 522, 1567, 2088
TspMI	CCCGGG	1 cut(s)	574
TspRI	CASTG	3 cut(s)	194, 709, 793
Tth111I	GACNNNGTC	1 cut(s)	528
Van91I	CCANNNNNTGG	1 cut(s)	1115
VneI	GTGCAC	1 cut(s)	72
VpaK11BI	GGWCC	5 cut(s)	136, 293, 1151, 1283, 1398
XagI	CCTNNNNNAGG	3 cut(s)	771, 863, 973
XapI	RAATTY	4 cut(s)	605, 899, 1344, 2011
XceI	RCATGY	3 cut(s)	1277, 1856, 1961
XcmI	CCANNNNNNNNNTGG	1 cut(s)	557
XmaI	CCCGGG	1 cut(s)	574
XmiI	GTMKAC	1 cut(s)	1668
XmnI	GAANNNNTTC	1 cut(s)	1830
XspI	CTAG	4 cut(s)	1602, 1712, 2054, 2250
ZrmI	AGTACT	1 cut(s)	1710
Zsp2I	ATGCAT	2 cut(s)	1686, 1854

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.