Rmu_sc0003765.1_g000035
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003765.1
OG0003287
Physical Location & Seq
Reverse (-)
137154 .. 139193
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
atggtctataactattgcagaagaagaaagagactgatgggagaagggtacaacaatccaaaggccaatcatgttagtgccaccagtgaaggaaaggaagatgcagtgacttttccactctcccagacagagtgtaatcaaatgctggggctgctaagccgtctccaagaagcagggaataaaacaaaggatgagagtcaattgctagagatgatgaacatcaacaaaccatcaacgaccaactttgtaggtaatgtaccaaaccttgaagatatctcaggtaaaatttgtgcactttatcgtcataacaaggatgtagtatggattttggatagcggggctagcgaccatattgacctacgttcggggaggatgattgggacgggagctgagagtgagggactatactgcctcaatatgcctcaggaaggaacgtgcaatgcggtgagcagcaaaaccggctatccaaatggacaaaagggctacaaagtctatcgtctgcatgatgcaaagattttggtctctagagacgtagtattctttgaaactgagtttccttttcagactagggtttcttctagtcttcactccacgacaccaccaaacatgttgtttactactcctactataccttctcatatcgaagatgatcttgtgcataccccaaatctctctgatcctaccatatcttctcctcaatcaccacatcactcggattctaccatatctccctcttctgcttcaccagatgatgagctgttttcttcttccactataccaccaccaaatccggatgttacttctccagatactatccctattcaggtctctacttcccaacctttgctccgacgttctacatgtgcaacaaagcctcctaccgctttgcaagacttccacattgagatggccttgccttcacgctctgtttccccatcttccatgagcaatgcagtgtcaggtacgccatatcctatctctgaggttatttcatatgacagactctctcccaagcatcgagcatttactgttaatctctcacacacgagaacctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

351

Amino Acids

38.54

Weight (kDa)

6.17

Isoelectric Point (pI)

64.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 154 - 190 2.6e-10 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 790
AciI CCGC 3 cut(s) 336, 443, 882
AclWI GGATC 1 cut(s) 671
AcsI RAATTY 1 cut(s) 285
AfaI GTAC 3 cut(s) 50, 258, 964
AfiI CCNNNNNNNGG 3 cut(s) 364, 428, 823
AflIII ACRYGT 2 cut(s) 606, 860
AgsI TTSAA 2 cut(s) 269, 545
AluBI AGCT 2 cut(s) 389, 757
AluI AGCT 2 cut(s) 389, 757
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 5 cut(s) 25, 167, 522, 526, 832
Alw44I GTGCAC 1 cut(s) 291
AlwI GGATC 1 cut(s) 671
Aor13HI TCCGGA 1 cut(s) 790
AoxI GGCC 2 cut(s) 63, 909
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 2 cut(s) 151, 450
ApoI RAATTY 1 cut(s) 285
AsuHPI GGTGA 3 cut(s) 457, 693, 735
AsuNHI GCTAGC 1 cut(s) 341
AxyI CCTNAGG 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 295
BauI CACGAG 1 cut(s) 1045
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 138, 462
BccI CCATC 4 cut(s) 31, 238, 901, 943
BceAI ACGGC 1 cut(s) 144
BcoDI GTCTC 5 cut(s) 25, 167, 522, 526, 832
BfaI CTAG 5 cut(s) 206, 342, 525, 567, 579
BisI GCNGC 2 cut(s) 152, 451
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 2 cut(s) 153, 452
BmsI GCATC 3 cut(s) 91, 496, 1024
BmtI GCTAGC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 789
Bpu1102I GCTNAGC 1 cut(s) 155
BsaBI GATNNNNATC 1 cut(s) 218
BsaI GGTCTC 2 cut(s) 526, 832
BsaWI WCCGGW 1 cut(s) 790
BsaXI ACNNNNNCTCC 6 cut(s) 712, 742, 831, 859, 861, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 428, 823
Bse118I RCCGGY 1 cut(s) 458
Bse1I ACTGG 1 cut(s) 84
Bse21I CCTNAGG 1 cut(s) 423
Bse3DI GCAATG 2 cut(s) 445, 955
Bse8I GATNNNNATC 1 cut(s) 218
BseAI TCCGGA 1 cut(s) 790
BseGI GGATG 4 cut(s) 196, 319, 378, 799
BseJI GATNNNNATC 1 cut(s) 218
BseLI CCNNNNNNNGG 3 cut(s) 364, 428, 823
BseMI GCAATG 2 cut(s) 445, 955
BseMII CTCAG 5 cut(s) 291, 381, 437, 540, 972
BseNI ACTGG 1 cut(s) 84
BseRI GAGGAG 1 cut(s) 684
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 2 cut(s) 138, 462
BseYI CCCAGC 1 cut(s) 145
BshFI GGCC 2 cut(s) 65, 911
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 2 cut(s) 459, 791
BslFI GGGAC 2 cut(s) 394, 414
BslI CCNNNNNNNGG 3 cut(s) 364, 428, 823
BsmAI GTCTC 5 cut(s) 25, 167, 522, 526, 832
BsmBI CGTCTC 2 cut(s) 167, 522
BsmFI GGGAC 2 cut(s) 394, 414
BsnI GGCC 2 cut(s) 65, 911
Bso31I GGTCTC 2 cut(s) 526, 832
Bsp1286I GDGCHC 1 cut(s) 295
Bsp13I TCCGGA 1 cut(s) 790
Bsp143I GATC 2 cut(s) 649, 676
Bsp1720I GCTNAGC 1 cut(s) 155
BspACI CCGC 3 cut(s) 336, 443, 882
BspANI GGCC 2 cut(s) 65, 911
BspCNI CTCAG 5 cut(s) 290, 382, 436, 541, 973
BspEI TCCGGA 1 cut(s) 790
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 1 cut(s) 671
BspTNI GGTCTC 2 cut(s) 526, 832
BsrDI GCAATG 2 cut(s) 445, 955
BsrFI RCCGGY 1 cut(s) 458
BsrI ACTGG 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 458
BssMI GATC 2 cut(s) 649, 676
BssSI CACGAG 1 cut(s) 1045
Bst2BI CACGAG 1 cut(s) 1045
Bst4CI ACNGT 1 cut(s) 1030
Bst6I CTCTTC 1 cut(s) 739
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 6 cut(s) 155, 277, 390, 423, 549, 981
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 4 cut(s) 196, 319, 378, 799
BstKTI GATC 2 cut(s) 652, 679
BstMAI GTCTC 5 cut(s) 25, 167, 522, 526, 832
BstMBI GATC 2 cut(s) 649, 676
BstMWI GCNNNNNNNGC 3 cut(s) 151, 342, 459
BstNSI RCATGY 2 cut(s) 610, 864
BstSLI GKGCMC 1 cut(s) 295
BstV1I GCAGC 2 cut(s) 138, 462
BstV2I GAAGAC 1 cut(s) 575
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 2 cut(s) 65, 911
BtsCI GGATG 4 cut(s) 196, 319, 378, 799
BtsI GCAGTG 2 cut(s) 111, 960
BtsIMutI CAGTG 3 cut(s) 91, 111, 960
Cac8I GCNNGC 1 cut(s) 343
Cfr10I RCCGGY 1 cut(s) 458
Csp6I GTAC 3 cut(s) 49, 257, 963
CviAII CATG 5 cut(s) 71, 503, 607, 861, 943
CviQI GTAC 3 cut(s) 49, 257, 963
DdeI CTNAG 6 cut(s) 155, 277, 390, 423, 549, 981
DpnI GATC 2 cut(s) 651, 678
DpnII GATC 2 cut(s) 649, 676
Eam1104I CTCTTC 1 cut(s) 739
EarI CTCTTC 1 cut(s) 739
Eco31I GGTCTC 2 cut(s) 526, 832
Eco32I GATATC 1 cut(s) 274
Eco81I CCTNAGG 1 cut(s) 423
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 274
Esp3I CGTCTC 2 cut(s) 167, 522
FaeI CATG 5 cut(s) 74, 506, 610, 864, 946
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 2 cut(s) 394, 414
FatI CATG 5 cut(s) 70, 502, 606, 860, 942
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 994
Fnu4HI GCNGC 2 cut(s) 152, 451
FokI GGATG 4 cut(s) 203, 326, 385, 806
Fsp4HI GCNGC 2 cut(s) 152, 451
FspBI CTAG 5 cut(s) 206, 342, 525, 567, 579
GluI GCNGC 2 cut(s) 152, 451
GsaI CCCAGC 1 cut(s) 149
GsuI CTGGAG 1 cut(s) 789
HaeIII GGCC 2 cut(s) 65, 911
HapII CCGG 2 cut(s) 459, 791
Hin1II CATG 5 cut(s) 74, 506, 610, 864, 946
HinfI GANTC 3 cut(s) 196, 716, 1002
HpaII CCGG 2 cut(s) 459, 791
HphI GGTGA 3 cut(s) 457, 693, 735
Hpy166II GTNNAC 2 cut(s) 293, 615
Hpy188I TCNGA 5 cut(s) 564, 676, 715, 851, 982
Hpy188III TCNNGA 4 cut(s) 425, 525, 791, 806
Hpy8I GTNNAC 2 cut(s) 293, 615
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 5 cut(s) 38, 83, 422, 642, 927
HpyCH4III ACNGT 1 cut(s) 1030
HpyCH4IV ACGT 4 cut(s) 361, 434, 531, 853
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 342, 459
HpyF3I CTNAG 6 cut(s) 155, 277, 390, 423, 549, 981
HpySE526I ACGT 4 cut(s) 361, 434, 531, 853
Hsp92II CATG 5 cut(s) 74, 506, 610, 864, 946
Kpn2I TCCGGA 1 cut(s) 790
Kzo9I GATC 2 cut(s) 649, 676
LmnI GCTCC 2 cut(s) 386, 852
Lsp1109I GCAGC 2 cut(s) 138, 462
LweI GCATC 3 cut(s) 91, 496, 1024
MaeI CTAG 5 cut(s) 206, 342, 525, 567, 579
MaeII ACGT 4 cut(s) 361, 434, 531, 853
MaeIII GTNAC 2 cut(s) 106, 796
MalI GATC 2 cut(s) 651, 678
MboI GATC 2 cut(s) 649, 676
MfeI CAATTG 1 cut(s) 200
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 2 cut(s) 200, 285
MlyI GAGTC 2 cut(s) 205, 996
MmeI TCCRAC 1 cut(s) 874
MnlI CCTC 8 cut(s) 363, 391, 422, 432, 705, 742, 885, 976
MroI TCCGGA 1 cut(s) 790
MseI TTAA 1 cut(s) 1032
MslI CAYNNNNRTG 2 cut(s) 75, 905
MspI CCGG 2 cut(s) 459, 791
MunI CAATTG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 151, 342, 459
NdeI CATATG 1 cut(s) 994
NdeII GATC 2 cut(s) 649, 676
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 5 cut(s) 74, 506, 610, 864, 946
NmuCI GTSAC 1 cut(s) 106
NspI RCATGY 2 cut(s) 610, 864
PciI ACATGT 2 cut(s) 606, 860
PfeI GAWTC 1 cut(s) 716
PkrI GCNGC 2 cut(s) 153, 452
PleI GAGTC 2 cut(s) 204, 996
PpsI GAGTC 2 cut(s) 204, 996
PscI ACATGT 2 cut(s) 606, 860
PspFI CCCAGC 1 cut(s) 145
RsaI GTAC 3 cut(s) 50, 258, 964
RsaNI GTAC 3 cut(s) 49, 257, 963
RseI CAYNNNNRTG 2 cut(s) 75, 905
SaqAI TTAA 1 cut(s) 1032
SatI GCNGC 2 cut(s) 152, 451
Sau3AI GATC 2 cut(s) 649, 676
SchI GAGTC 2 cut(s) 205, 996
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 3 cut(s) 91, 496, 1024
SmiMI CAYNNNNRTG 2 cut(s) 75, 905
Sse9I AATT 2 cut(s) 200, 285
SsiI CCGC 3 cut(s) 336, 443, 882
SspMI CTAG 5 cut(s) 206, 342, 525, 567, 579
TaaI ACNGT 1 cut(s) 1030
TaiI ACGT 4 cut(s) 364, 437, 534, 856
TaqI TCGA 2 cut(s) 642, 1018
TasI AATT 2 cut(s) 200, 285
TfiI GAWTC 1 cut(s) 716
Tru1I TTAA 1 cut(s) 1032
Tru9I TTAA 1 cut(s) 1032
TscAI CASTG 3 cut(s) 91, 111, 960
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 151, 450
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 2 cut(s) 230, 981
TspRI CASTG 3 cut(s) 91, 111, 960
VneI GTGCAC 1 cut(s) 291
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 1 cut(s) 285
XbaI TCTAGA 1 cut(s) 524
XceI RCATGY 2 cut(s) 610, 864
XspI CTAG 5 cut(s) 206, 342, 525, 567, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.