Rmu_sc0004031.1_g000007

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004031.1
OG0005210
Physical Location & Seq
Forward (+)
36622 .. 40052
3431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004031.1_g000007.1.cds

Sequence Viewer

Length: 1086 bp
atgaaacaaattctctcgagctctctcagttgtgaaaccccccgtctctccaccacccctccccacaactctcccaatagtgaaacaacccccccacgaaacccctctctctcagtctctcgatctctcttgtctcaatttcatgctctcttcttcttcaatctcatcaccccctttcaaaccccaaccctaaaatcttcctcccccagcaatggcctcctccctcgaccgcaaccacaccctgaacgacgattccaacacccacgcctccaaaaaacccaaactctccttcatcacctcctccgaaatcgccgccgaattctcccaccacgaccccaccgtcgcccgaatcaacaacggcagttcgggtcctgccccgactcagtcctccaggcccagcacccctggcagctcaagtacctctcccagcccgatcacttctacttcaaccacctccaagccggcatcctcatcaaggacctcatcaacgccgaccacgtcgacgaggtctccatcgtcgacaatgccaccaccgccgccgccatcgtcctccagcaggccgcctggggcttctccgagagcaagttcgagcagggcgacgccgtcgtcatgctccactgcgcctacggcgccgtcaagaagtcgattgaggcctacgtcactcgcgccggcgggcacgtcattgagcaccttagagaagaacacttggaagctgattcaagagcgcaggacctctctgcagagcgagctgaagctcaacagaagttggatcaagaaattaaagagcgtgaagagaagtactctgatcttgactcgaaattcaccaggcttcacaaacgagcaaagcagtgtattctagatgttcaaaaggagattacaaaggagacatggttgctcaccgaggaagggaagacatatgctgttcatggaacgccagaagtgtggctcttcctgtccataccgcaagagggcatcacgaaagatgaattgcaggagcggtcaactggccgatctcgtggatttggatatgtaacgtttgcatcagctgaggatgccaaggtgagttggattttcagttctggttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

361

Amino Acids

41.08

Weight (kDa)

8.58

Isoelectric Point (pI)

58.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 339, 605
AccB1I GGYRCC 1 cut(s) 629
AccBSI CCGCTC 1 cut(s) 997
AccI GTMKAC 2 cut(s) 501, 519
AccII CGCG 1 cut(s) 666
AciI CCGC 9 cut(s) 230, 313, 534, 537, 540, 561, 672, 962, 997
AclI AACGTT 1 cut(s) 1034
AclWI GGATC 1 cut(s) 777
AcoI YGGCCR 1 cut(s) 1006
AcsI RAATTY 3 cut(s) 9, 318, 818
AcuI CTGAAG 1 cut(s) 771
AcyI GRCGYC 2 cut(s) 600, 630
AfaI GTAC 2 cut(s) 419, 800
AfiI CCNNNNNNNGG 5 cut(s) 212, 475, 556, 906, 968
AgsI TTSAA 5 cut(s) 160, 179, 448, 720, 866
AjiI CACGTC 2 cut(s) 499, 679
AjnI CCWGG 4 cut(s) 390, 404, 563, 824
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AloI GAACNNNNNNTCC 3 cut(s) 237, 269, 1060
AluBI AGCT 6 cut(s) 21, 412, 713, 749, 755, 1046
AluI AGCT 6 cut(s) 21, 412, 713, 749, 755, 1046
Alw21I GWGCWC 2 cut(s) 23, 690
Alw26I GTCTC 5 cut(s) 50, 121, 138, 514, 878
AlwI GGATC 1 cut(s) 777
Ama87I CYCGRG 1 cut(s) 16
AoxI GGCC 5 cut(s) 214, 393, 558, 651, 1006
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 3 cut(s) 9, 318, 818
AspLEI GCGC 4 cut(s) 623, 632, 668, 727
AspS9I GGNCC 4 cut(s) 369, 394, 478, 730
AsuHPI GGTGA 5 cut(s) 160, 287, 814, 889, 1072
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 3 cut(s) 369, 478, 730
BaeGI GKGCMC 1 cut(s) 678
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 23
BauI CACGAG 1 cut(s) 1014
BbsI GAAGAC 1 cut(s) 917
Bbv12I GWGCWC 2 cut(s) 23, 690
BbvCI CCTCAGC 1 cut(s) 1047
BbvI GCAGC 1 cut(s) 421
BccI CCATC 2 cut(s) 521, 551
BceAI ACGGC 4 cut(s) 374, 587, 617, 643
BciT130I CCWGG 4 cut(s) 392, 406, 565, 826
BcoDI GTCTC 5 cut(s) 50, 121, 138, 514, 878
BfaI CTAG 1 cut(s) 857
BfmI CTRYAG 1 cut(s) 738
BfoI RGCGCY 1 cut(s) 633
BisI GCNGC 5 cut(s) 313, 410, 537, 540, 561
BlsI GCNGC 5 cut(s) 314, 411, 538, 541, 562
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 4 cut(s) 392, 406, 565, 826
Bme18I GGWCC 3 cut(s) 369, 478, 730
BmeT110I CYCGRG 1 cut(s) 16
BmgBI CACGTC 2 cut(s) 499, 679
BmgT120I GGNCC 4 cut(s) 369, 394, 478, 730
BmiI GGNNCC 2 cut(s) 370, 631
BmrFI CCNGG 4 cut(s) 392, 406, 565, 826
BmsI GCATC 4 cut(s) 474, 981, 1042, 1049
BpiI GAAGAC 1 cut(s) 917
BplI GAGNNNNNCTC 2 cut(s) 785, 817
BpmI CTGGAG 2 cut(s) 374, 536
Bpu10I CCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 1 cut(s) 398
BsaHI GRCGYC 2 cut(s) 600, 630
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 4 cut(s) 404, 564, 900, 1056
BsaXI ACNNNNNCTCC 6 cut(s) 43, 73, 494, 524, 875, 905
Bsc4I CCNNNNNNNGG 5 cut(s) 212, 475, 556, 906, 968
Bse118I RCCGGY 2 cut(s) 461, 668
Bse1I ACTGG 1 cut(s) 1009
Bse3DI GCAATG 1 cut(s) 217
BseBI CCWGG 4 cut(s) 392, 406, 565, 826
BseDI CCNNGG 4 cut(s) 404, 564, 900, 1056
BseGI GGATG 2 cut(s) 465, 1057
BseLI CCNNNNNNNGG 5 cut(s) 212, 475, 556, 906, 968
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 4 cut(s) 40, 126, 396, 1038
BseNI ACTGG 1 cut(s) 1009
BseRI GAGGAG 2 cut(s) 209, 290
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 1 cut(s) 421
BseYI CCCAGC 3 cut(s) 206, 396, 426
Bsh1236I CGCG 1 cut(s) 666
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 5 cut(s) 216, 395, 560, 653, 1008
BshNI GGYRCC 1 cut(s) 629
BsiEI CGRYCG 1 cut(s) 230
BsiHKAI GWGCWC 2 cut(s) 23, 690
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 2 cut(s) 462, 669
BslI CCNNNNNNNGG 5 cut(s) 212, 475, 556, 906, 968
BsmAI GTCTC 5 cut(s) 50, 121, 138, 514, 878
BsmBI CGTCTC 1 cut(s) 50
BsnI GGCC 5 cut(s) 216, 395, 560, 653, 1008
Bso31I GGTCTC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 16
Bsp1286I GDGCHC 3 cut(s) 23, 678, 690
Bsp143I GATC 5 cut(s) 122, 433, 769, 805, 1010
BspACI CCGC 9 cut(s) 230, 313, 534, 537, 540, 561, 672, 962, 997
BspANI GGCC 5 cut(s) 216, 395, 560, 653, 1008
BspCNI CTCAG 4 cut(s) 39, 125, 395, 1039
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 2 cut(s) 370, 631
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 1 cut(s) 777
BspQI GCTCTTC 1 cut(s) 953
BspT107I GGYRCC 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 997
BsrDI GCAATG 1 cut(s) 217
BsrFI RCCGGY 2 cut(s) 461, 668
BsrI ACTGG 1 cut(s) 1009
BssAI RCCGGY 2 cut(s) 461, 668
BssECI CCNNGG 4 cut(s) 404, 564, 900, 1056
BssMI GATC 5 cut(s) 122, 433, 769, 805, 1010
BssNI GRCGYC 2 cut(s) 600, 630
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 1 cut(s) 1056
Bst2BI CACGAG 1 cut(s) 1014
Bst2UI CCWGG 4 cut(s) 392, 406, 565, 826
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 3 cut(s) 155, 786, 953
BstACI GRCGYC 2 cut(s) 600, 630
BstC8I GCNNGC 5 cut(s) 463, 558, 670, 674, 747
BstDEI CTNAG 5 cut(s) 26, 112, 382, 692, 1047
BstENI CCTNNNNNAGG 2 cut(s) 473, 554
BstF5I GGATG 2 cut(s) 465, 1057
BstFNI CGCG 1 cut(s) 666
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 4 cut(s) 623, 632, 668, 727
BstKTI GATC 5 cut(s) 125, 436, 772, 808, 1013
BstMAI GTCTC 5 cut(s) 50, 121, 138, 514, 878
BstMBI GATC 5 cut(s) 122, 433, 769, 805, 1010
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 6 cut(s) 406, 533, 627, 629, 746, 1052
BstNI CCWGG 4 cut(s) 392, 406, 565, 826
BstSCI CCNGG 4 cut(s) 390, 404, 563, 824
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 917
BstXI CCANNNNNNTGG 1 cut(s) 942
BsuRI GGCC 5 cut(s) 216, 395, 560, 653, 1008
BtrI CACGTC 2 cut(s) 499, 679
BtsCI GGATG 2 cut(s) 465, 1057
BtsI GCAGTG 2 cut(s) 616, 854
BtsIMutI CAGTG 2 cut(s) 616, 854
Cac8I GCNNGC 5 cut(s) 463, 558, 670, 674, 747
CfoI GCGC 4 cut(s) 623, 632, 668, 727
Cfr10I RCCGGY 2 cut(s) 461, 668
Cfr13I GGNCC 4 cut(s) 369, 394, 478, 730
CseI GACGC 1 cut(s) 608
Csp6I GTAC 2 cut(s) 418, 799
CviAII CATG 4 cut(s) 143, 610, 888, 926
CviQI GTAC 2 cut(s) 418, 799
DdeI CTNAG 5 cut(s) 26, 112, 382, 692, 1047
DinI GGCGCC 1 cut(s) 631
DpnI GATC 5 cut(s) 124, 435, 771, 807, 1012
DpnII GATC 5 cut(s) 122, 433, 769, 805, 1010
DrdI GACNNNNNNGTC 2 cut(s) 339, 605
DseDI GACNNNNNNGTC 2 cut(s) 339, 605
EaeI YGGCCR 1 cut(s) 1006
Eam1104I CTCTTC 3 cut(s) 155, 786, 953
EarI CTCTTC 3 cut(s) 155, 786, 953
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 1056
Eco147I AGGCCT 1 cut(s) 653
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 514
Eco47I GGWCC 3 cut(s) 369, 478, 730
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 21
EcoNI CCTNNNNNAGG 2 cut(s) 473, 554
EcoO109I RGGNCCY 3 cut(s) 369, 478, 730
EcoRI GAATTC 1 cut(s) 318
EcoRII CCWGG 4 cut(s) 390, 404, 563, 824
EcoT14I CCWWGG 1 cut(s) 1056
EcoT38I GRGCYC 1 cut(s) 23
EgeI GGCGCC 1 cut(s) 631
EheI GGCGCC 1 cut(s) 631
ErhI CCWWGG 1 cut(s) 1056
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 4 cut(s) 146, 613, 891, 929
FaiI YATR 8 cut(s) 144, 611, 889, 916, 918, 927, 959, 1029
FatI CATG 4 cut(s) 142, 609, 887, 925
FauI CCCGC 1 cut(s) 665
FauNDI CATATG 1 cut(s) 916
FblI GTMKAC 2 cut(s) 501, 519
Fnu4HI GCNGC 5 cut(s) 313, 410, 537, 540, 561
FokI GGATG 2 cut(s) 452, 1064
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 5 cut(s) 313, 410, 537, 540, 561
FspBI CTAG 1 cut(s) 857
GlaI GCGC 4 cut(s) 622, 631, 667, 726
GluI GCNGC 5 cut(s) 313, 410, 537, 540, 561
GsaI CCCAGC 3 cut(s) 210, 400, 430
GsuI CTGGAG 2 cut(s) 374, 536
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 5 cut(s) 216, 395, 560, 653, 1008
HapII CCGG 2 cut(s) 462, 669
HgaI GACGC 1 cut(s) 608
HhaI GCGC 4 cut(s) 623, 632, 668, 727
Hin1I GRCGYC 2 cut(s) 600, 630
Hin1II CATG 4 cut(s) 146, 613, 891, 929
Hin6I GCGC 4 cut(s) 621, 630, 666, 725
HinP1I GCGC 4 cut(s) 621, 630, 666, 725
HincII GTYRAC 3 cut(s) 502, 520, 1002
HindII GTYRAC 3 cut(s) 502, 520, 1002
HinfI GANTC 5 cut(s) 252, 349, 380, 716, 812
HpaII CCGG 2 cut(s) 462, 669
HphI GGTGA 5 cut(s) 160, 287, 814, 889, 1072
Hpy166II GTNNAC 3 cut(s) 502, 520, 1002
Hpy188I TCNGA 3 cut(s) 305, 577, 805
Hpy188III TCNNGA 8 cut(s) 16, 120, 637, 720, 773, 809, 857, 976
Hpy8I GTNNAC 3 cut(s) 502, 520, 1002
Hpy99I CGWCG 7 cut(s) 252, 345, 503, 506, 521, 602, 608
HpyAV CCTTC 2 cut(s) 299, 899
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 4 cut(s) 498, 657, 678, 1034
HpyCH4V TGCA 3 cut(s) 740, 991, 1040
HpyF10VI GCNNNNNNNGC 6 cut(s) 406, 533, 627, 629, 746, 1052
HpyF3I CTNAG 5 cut(s) 26, 112, 382, 692, 1047
HpySE526I ACGT 4 cut(s) 498, 657, 678, 1034
Hsp92I GRCGYC 2 cut(s) 600, 630
Hsp92II CATG 4 cut(s) 146, 613, 891, 929
HspAI GCGC 4 cut(s) 621, 630, 666, 725
KasI GGCGCC 1 cut(s) 629
KroI GCCGGC 2 cut(s) 461, 668
KroNI GCCGGC 2 cut(s) 463, 670
Kzo9I GATC 5 cut(s) 122, 433, 769, 805, 1010
LguI GCTCTTC 1 cut(s) 953
LmnI GCTCC 2 cut(s) 618, 994
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 4 cut(s) 474, 981, 1042, 1049
MaeI CTAG 1 cut(s) 857
MaeII ACGT 4 cut(s) 498, 657, 678, 1034
MaeIII GTNAC 2 cut(s) 658, 1030
MalI GATC 5 cut(s) 124, 435, 771, 807, 1012
MbiI CCGCTC 1 cut(s) 997
MboI GATC 5 cut(s) 122, 433, 769, 805, 1010
MboII GAAGA 8 cut(s) 142, 145, 148, 189, 710, 803, 922, 940
MhlI GDGCHC 3 cut(s) 23, 678, 690
MluCI AATT 6 cut(s) 9, 137, 318, 777, 818, 986
Mly113I GGCGCC 1 cut(s) 630
MlyI GAGTC 2 cut(s) 374, 806
MmeI TCCRAC 3 cut(s) 280, 747, 1046
MreI CGCCGGCG 1 cut(s) 668
MroNI GCCGGC 2 cut(s) 461, 668
MseI TTAA 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 1046
MspI CCGG 2 cut(s) 462, 669
MspR9I CCNGG 4 cut(s) 392, 406, 565, 826
MvaI CCWGG 4 cut(s) 392, 406, 565, 826
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 6 cut(s) 406, 533, 627, 629, 746, 1052
NaeI GCCGGC 2 cut(s) 463, 670
NarI GGCGCC 1 cut(s) 630
NdeI CATATG 1 cut(s) 916
NdeII GATC 5 cut(s) 122, 433, 769, 805, 1010
NgoMIV GCCGGC 2 cut(s) 461, 668
NlaIII CATG 4 cut(s) 146, 613, 891, 929
NlaIV GGNNCC 2 cut(s) 370, 631
NmuCI GTSAC 1 cut(s) 658
PaeR7I CTCGAG 1 cut(s) 16
PceI AGGCCT 1 cut(s) 653
PciSI GCTCTTC 1 cut(s) 953
PcsI WCGNNNNNNNCGW 3 cut(s) 337, 495, 594
PdiI GCCGGC 2 cut(s) 463, 670
PfeI GAWTC 3 cut(s) 252, 349, 716
PflFI GACNNNGTC 4 cut(s) 383, 497, 506, 602
PkrI GCNGC 5 cut(s) 314, 411, 538, 541, 562
PleI GAGTC 2 cut(s) 374, 806
PluTI GGCGCC 1 cut(s) 633
PpsI GAGTC 2 cut(s) 374, 806
PpuMI RGGWCCY 3 cut(s) 369, 478, 730
Psp124BI GAGCTC 1 cut(s) 23
Psp1406I AACGTT 1 cut(s) 1034
Psp5II RGGWCCY 3 cut(s) 369, 478, 730
Psp6I CCWGG 4 cut(s) 390, 404, 563, 824
PspFI CCCAGC 3 cut(s) 206, 396, 426
PspGI CCWGG 4 cut(s) 390, 404, 563, 824
PspN4I GGNNCC 2 cut(s) 370, 631
PspPI GGNCC 4 cut(s) 369, 394, 478, 730
PspPPI RGGWCCY 3 cut(s) 369, 478, 730
PstI CTGCAG 1 cut(s) 742
PsyI GACNNNGTC 4 cut(s) 383, 497, 506, 602
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 2 cut(s) 419, 800
RsaNI GTAC 2 cut(s) 418, 799
SacI GAGCTC 1 cut(s) 23
SalI GTCGAC 2 cut(s) 500, 518
SapI GCTCTTC 1 cut(s) 953
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 5 cut(s) 313, 410, 537, 540, 561
Sau3AI GATC 5 cut(s) 122, 433, 769, 805, 1010
Sau96I GGNCC 4 cut(s) 369, 394, 478, 730
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 2 cut(s) 374, 806
ScrFI CCNGG 4 cut(s) 392, 406, 565, 826
SduI GDGCHC 3 cut(s) 23, 678, 690
SfaNI GCATC 4 cut(s) 474, 981, 1042, 1049
SfcI CTRYAG 1 cut(s) 738
SfoI GGCGCC 1 cut(s) 631
Sfr274I CTCGAG 1 cut(s) 16
SgrAI CRCCGGYG 1 cut(s) 668
SgrDI CGTCGACG 1 cut(s) 500
SinI GGWCC 3 cut(s) 369, 478, 730
SlaI CTCGAG 1 cut(s) 16
SmlI CTYRAG 2 cut(s) 16, 413
SmoI CTYRAG 2 cut(s) 16, 413
Sse9I AATT 6 cut(s) 9, 137, 318, 777, 818, 986
SseBI AGGCCT 1 cut(s) 653
SsiI CCGC 9 cut(s) 230, 313, 534, 537, 540, 561, 672, 962, 997
SspDI GGCGCC 1 cut(s) 629
SspMI CTAG 1 cut(s) 857
SstI GAGCTC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 653
StyD4I CCNGG 4 cut(s) 390, 404, 563, 824
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 4 cut(s) 501, 660, 681, 1037
TaqI TCGA 8 cut(s) 17, 121, 226, 501, 519, 588, 644, 815
TasI AATT 6 cut(s) 9, 137, 318, 777, 818, 986
TatI WGTACW 1 cut(s) 798
TauI GCSGC 4 cut(s) 315, 539, 542, 563
TfiI GAWTC 3 cut(s) 252, 349, 716
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 2 cut(s) 623, 854
TseFI GTSAC 1 cut(s) 658
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 658
TspDTI ATGAA 5 cut(s) 17, 131, 281, 914, 999
TspRI CASTG 2 cut(s) 623, 854
Tth111I GACNNNGTC 4 cut(s) 383, 497, 506, 602
VpaK11BI GGWCC 3 cut(s) 369, 478, 730
XagI CCTNNNNNAGG 2 cut(s) 473, 554
XapI RAATTY 3 cut(s) 9, 318, 818
XbaI TCTAGA 1 cut(s) 856
XhoI CTCGAG 1 cut(s) 16
XmiI GTMKAC 2 cut(s) 501, 519
XspI CTAG 1 cut(s) 857
ZrmI AGTACT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.