Rorug06G0311200

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005210
Physical Location & Seq
Reverse (-)
46248895 .. 46249440
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0311200.1

Sequence Viewer

Length: 546 bp
ATGGATCTTACATACTACAAGGGAAATTTCTATGTTGTGGACCTATCTGGCCGTGTTTCTGTTGTTTGTGAAATTGAAGACCCTAAGCGAGTACATTTGAGTGTTGTTGCTCCAGAGATTATGAATAAGGAGGAACTTCTTGGTCCAATGAAGGGTATCAAACAGCTATATCTGGTGGAATCAGCAGGGTCCCTATTGGTGGTTTTGTGTACTTACTACCATAATCATTATGAATCAACTATTGGATGCAGGGTTTTCAAGGTCCCATTTAGTAATGGCAATTCATGGTCCAACTTAGAGGTAAAGAATTTGGGTAATAGAGCCCTCTTCTTGAGTAAAAGCAGTTCTTCCTTCTCTGTTGAGGTCTCAGGCAGTTTCGGATGCAAGGCCAATTGCATATATTTCCTGAATAACAAAACAATCTCTCCATTCCTTGACTTAAGTGTTGGTATTTTTAATATGGAGGATGGGACAATCGATCAAGGCTTTAGCAGTTCCTTCAATACCGGGATTGAAGAAATCAGAGAATTCCATCTATGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

181

Amino Acids

20.28

Weight (kDa)

5.56

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 2 - 153 7e-25 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 3 cut(s) 25, 307, 527
AfaI GTAC 2 cut(s) 93, 211
AfiI CCNNNNNNNGG 2 cut(s) 152, 199
AflII CTTAAG 1 cut(s) 439
AgsI TTSAA 4 cut(s) 77, 259, 502, 515
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
Alw26I GTCTC 1 cut(s) 370
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 49, 387
ApoI RAATTY 3 cut(s) 25, 307, 527
AspS9I GGNCC 5 cut(s) 40, 143, 189, 262, 288
AsuC2I CCSGG 1 cut(s) 508
AvaII GGWCC 5 cut(s) 40, 143, 189, 262, 288
BanII GRGCYC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 84
BccI CCATC 2 cut(s) 461, 540
BceAI ACGGC 1 cut(s) 36
BcnI CCSGG 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 370
BfrI CTTAAG 1 cut(s) 439
Bme1390I CCNGG 1 cut(s) 508
Bme18I GGWCC 5 cut(s) 40, 143, 189, 262, 288
BmgT120I GGNCC 5 cut(s) 40, 143, 189, 262, 288
BmiI GGNNCC 3 cut(s) 190, 191, 264
BmrFI CCNGG 1 cut(s) 508
BmsI GCATC 2 cut(s) 236, 371
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 508
Bsa29I ATCGAT 1 cut(s) 477
BsaI GGTCTC 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 409, 439
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 199
BseCI ATCGAT 1 cut(s) 477
BseGI GGATG 3 cut(s) 251, 386, 472
BseLI CCNNNNNNNGG 2 cut(s) 152, 199
BseMII CTCAG 1 cut(s) 381
BshFI GGCC 2 cut(s) 51, 389
BshVI ATCGAT 1 cut(s) 477
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 3 cut(s) 175, 248, 484
BslI CCNNNNNNNGG 2 cut(s) 152, 199
BsmAI GTCTC 1 cut(s) 370
BsmFI GGGAC 3 cut(s) 175, 248, 484
BsnI GGCC 2 cut(s) 51, 389
Bso31I GGTCTC 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 2 cut(s) 4, 478
BspANI GGCC 2 cut(s) 51, 389
BspCNI CTCAG 1 cut(s) 380
BspDI ATCGAT 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 190, 191, 264
BspPI GGATC 1 cut(s) 12
BspTI CTTAAG 1 cut(s) 439
BspTNI GGTCTC 1 cut(s) 370
BssMI GATC 2 cut(s) 4, 478
Bst6I CTCTTC 1 cut(s) 332
BstAFI CTTAAG 1 cut(s) 439
BstDEI CTNAG 3 cut(s) 84, 295, 367
BstF5I GGATG 3 cut(s) 251, 386, 472
BstKTI GATC 2 cut(s) 7, 481
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 2 cut(s) 4, 478
BstSCI CCNGG 1 cut(s) 506
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 477
BsuRI GGCC 2 cut(s) 51, 389
BsuTUI ATCGAT 1 cut(s) 477
BtsCI GGATG 3 cut(s) 251, 386, 472
Cfr13I GGNCC 5 cut(s) 40, 143, 189, 262, 288
ClaI ATCGAT 1 cut(s) 477
Csp6I GTAC 2 cut(s) 92, 210
CviAII CATG 1 cut(s) 285
CviJI RGCY 5 cut(s) 51, 166, 323, 389, 486
CviKI_1 RGCY 5 cut(s) 51, 166, 323, 389, 486
CviQI GTAC 2 cut(s) 92, 210
DdeI CTNAG 3 cut(s) 84, 295, 367
DpnI GATC 2 cut(s) 6, 480
DpnII GATC 2 cut(s) 4, 478
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 370
Eco47I GGWCC 5 cut(s) 40, 143, 189, 262, 288
EcoO109I RGGNCCY 2 cut(s) 189, 262
EcoRI GAATTC 1 cut(s) 527
EcoT38I GRGCYC 1 cut(s) 325
FaeI CATG 1 cut(s) 288
FalI AAGNNNNNCTT 2 cut(s) 331, 363
FaqI GGGAC 3 cut(s) 175, 248, 484
FatI CATG 1 cut(s) 284
FokI GGATG 3 cut(s) 258, 393, 479
FriOI GRGCYC 1 cut(s) 325
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 51, 389
HapII CCGG 1 cut(s) 507
Hin1II CATG 1 cut(s) 288
HinfI GANTC 2 cut(s) 179, 233
HpaII CCGG 1 cut(s) 507
Hpy166II GTNNAC 2 cut(s) 40, 210
Hpy188I TCNGA 2 cut(s) 380, 524
Hpy188III TCNNGA 3 cut(s) 113, 331, 406
Hpy8I GTNNAC 2 cut(s) 40, 210
HpyAV CCTTC 3 cut(s) 145, 361, 508
HpyCH4V TGCA 3 cut(s) 249, 384, 396
HpyF3I CTNAG 3 cut(s) 84, 295, 367
Hsp92II CATG 1 cut(s) 288
KflI GGGWCCC 1 cut(s) 189
Kzo9I GATC 2 cut(s) 4, 478
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 8 cut(s) 33, 126, 158, 171, 235, 354, 419, 520
LweI GCATC 2 cut(s) 236, 371
MalI GATC 2 cut(s) 6, 480
MboI GATC 2 cut(s) 4, 478
MboII GAAGA 4 cut(s) 89, 319, 339, 527
MfeI CAATTG 1 cut(s) 391
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 325
MluCI AATT 6 cut(s) 25, 72, 280, 307, 391, 527
MmeI TCCRAC 1 cut(s) 315
MnlI CCTC 5 cut(s) 124, 292, 335, 355, 457
MseI TTAA 3 cut(s) 440, 456, 544
MslI CAYNNNNRTG 1 cut(s) 99
MspCI CTTAAG 1 cut(s) 439
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 1 cut(s) 508
MunI CAATTG 1 cut(s) 391
NciI CCSGG 1 cut(s) 508
NdeII GATC 2 cut(s) 4, 478
NlaIII CATG 1 cut(s) 288
NlaIV GGNNCC 3 cut(s) 190, 191, 264
PfeI GAWTC 2 cut(s) 179, 233
PpuMI RGGWCCY 2 cut(s) 189, 262
Psp5II RGGWCCY 2 cut(s) 189, 262
PspN4I GGNNCC 3 cut(s) 190, 191, 264
PspPI GGNCC 5 cut(s) 40, 143, 189, 262, 288
PspPPI RGGWCCY 2 cut(s) 189, 262
PsrI GAACNNNNNNTAC 2 cut(s) 328, 360
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 93, 211
RsaNI GTAC 2 cut(s) 92, 210
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 3 cut(s) 440, 456, 544
Sau3AI GATC 2 cut(s) 4, 478
Sau96I GGNCC 5 cut(s) 40, 143, 189, 262, 288
ScrFI CCNGG 1 cut(s) 508
SduI GDGCHC 1 cut(s) 325
SetI ASST 5 cut(s) 45, 168, 264, 303, 366
SfaNI GCATC 2 cut(s) 236, 371
SinI GGWCC 5 cut(s) 40, 143, 189, 262, 288
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 2 cut(s) 331, 439
SmoI CTYRAG 2 cut(s) 331, 439
Sse9I AATT 6 cut(s) 25, 72, 280, 307, 391, 527
StyD4I CCNGG 1 cut(s) 506
TaqI TCGA 1 cut(s) 477
TasI AATT 6 cut(s) 25, 72, 280, 307, 391, 527
TatI WGTACW 2 cut(s) 91, 209
TfiI GAWTC 2 cut(s) 179, 233
Tru1I TTAA 3 cut(s) 440, 456, 544
Tru9I TTAA 3 cut(s) 440, 456, 544
TspDTI ATGAA 4 cut(s) 137, 164, 246, 273
Vha464I CTTAAG 1 cut(s) 439
VpaK11BI GGWCC 5 cut(s) 40, 143, 189, 262, 288
XapI RAATTY 3 cut(s) 25, 307, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.