Rmu_sc0004695.1_g000009

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004695.1
OG0002234
Physical Location & Seq
Reverse (-)
30577 .. 32244
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
atgttgacggatcgggttgtagtcggttttgcagtggaaaaaactgtgaaccgaaccgagaccgattactcgggttcggtctcggctaaagagagagccgccgttgcagaacctcacccacacagcgcaatggccagaatcaaggtacacgagctgaggcagaagtcgaaggccgatcttttggcccagctcaaggatctcaaggcggagctggccctcctccgcgtcgccaaggtcaccggtgtagcacccaacaagctctccaagattaaggttgtgaggcttgggattgctcaggtgttgacagtgatcttgcagaagcagaaggctgcgttgagggaggtgtacaagagcaagaagctcttgcctcttgatcttcgtcccaagaagaccagggccattcgccgaaagctcaccaagcaccaggcatctttgaagactgaacgtgagaagaagagggagatgtactaccccttgaggaagtttgccatcaaggcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

166

Amino Acids

18.8

Weight (kDa)

10.62

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 51 - 107 3.1e-16 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 225
AciI CCGC 3 cut(s) 99, 206, 223
AclWI GGATC 2 cut(s) 18, 204
AcoI YGGCCR 1 cut(s) 132
AfaI GTAC 3 cut(s) 147, 347, 467
AfiI CCNNNNNNNGG 1 cut(s) 193
AgeI ACCGGT 1 cut(s) 239
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 2 cut(s) 392, 423
AluBI AGCT 6 cut(s) 154, 190, 211, 259, 361, 412
AluI AGCT 6 cut(s) 154, 190, 211, 259, 361, 412
Alw26I GTCTC 2 cut(s) 53, 85
AlwI GGATC 2 cut(s) 18, 204
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 5 cut(s) 132, 171, 183, 213, 396
ApeKI GCWGC 1 cut(s) 329
AsiGI ACCGGT 1 cut(s) 239
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 3 cut(s) 184, 214, 396
AsuHPI GGTGA 3 cut(s) 107, 229, 406
AvaI CYCGRG 1 cut(s) 70
BalI TGGCCA 1 cut(s) 134
BauI CACGAG 1 cut(s) 149
BbsI GAAGAC 2 cut(s) 395, 443
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 316
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 2 cut(s) 394, 425
BcoDI GTCTC 2 cut(s) 53, 85
BglI GCCNNNNNGGC 1 cut(s) 494
BisI GCNGC 2 cut(s) 99, 330
BlsI GCNGC 2 cut(s) 100, 331
Bme1390I CCNGG 2 cut(s) 394, 425
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 184, 214, 396
BmrFI CCNGG 2 cut(s) 394, 425
BmsI GCATC 1 cut(s) 437
BpiI GAAGAC 2 cut(s) 395, 443
Bpu10I CCTNAGC 2 cut(s) 155, 294
BpuEI CTTGAG 3 cut(s) 176, 185, 496
BsaI GGTCTC 2 cut(s) 53, 85
BsaJI CCNNGG 2 cut(s) 231, 393
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse118I RCCGGY 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 2 cut(s) 394, 425
BseDI CCNNGG 2 cut(s) 231, 393
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 2 cut(s) 146, 308
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 316
BseYI CCCAGC 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 5 cut(s) 134, 173, 185, 215, 398
BshTI ACCGGT 1 cut(s) 239
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 2 cut(s) 53, 85
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 5 cut(s) 134, 173, 185, 215, 398
Bso31I GGTCTC 2 cut(s) 53, 85
BsoBI CYCGRG 1 cut(s) 70
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 5 cut(s) 10, 175, 196, 309, 373
BspACI CCGC 3 cut(s) 99, 206, 223
BspANI GGCC 5 cut(s) 134, 173, 185, 215, 398
BspCNI CTCAG 2 cut(s) 147, 307
BspFNI CGCG 1 cut(s) 225
BspPI GGATC 2 cut(s) 18, 204
BspTNI GGTCTC 2 cut(s) 53, 85
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 239
BsrGI TGTACA 1 cut(s) 345
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 231, 393
BssMI GATC 5 cut(s) 10, 175, 196, 309, 373
BssSI CACGAG 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 231
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 2 cut(s) 394, 425
Bst4CI ACNGT 2 cut(s) 46, 307
Bst6I CTCTTC 1 cut(s) 449
BstAUI TGTACA 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 2 cut(s) 155, 294
BstEII GGTNACC 1 cut(s) 235
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 5 cut(s) 13, 178, 199, 312, 376
BstMAI GTCTC 2 cut(s) 53, 85
BstMBI GATC 5 cut(s) 10, 175, 196, 309, 373
BstMWI GCNNNNNNNGC 4 cut(s) 104, 212, 418, 494
BstNI CCWGG 2 cut(s) 394, 425
BstPI GGTNACC 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 392, 423
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 316
BstV2I GAAGAC 2 cut(s) 395, 443
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 5 cut(s) 134, 173, 185, 215, 398
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 39, 312
Cac8I GCNNGC 1 cut(s) 213
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 184, 214, 396
CseI GACGC 1 cut(s) 214
Csp6I GTAC 3 cut(s) 146, 346, 466
CspAI ACCGGT 1 cut(s) 239
CviQI GTAC 3 cut(s) 146, 346, 466
DdeI CTNAG 2 cut(s) 155, 294
DpnI GATC 5 cut(s) 12, 177, 198, 311, 375
DpnII GATC 5 cut(s) 10, 175, 196, 309, 373
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
EciI GGCGGA 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 231
Eco31I GGTCTC 2 cut(s) 53, 85
Eco88I CYCGRG 1 cut(s) 70
Eco91I GGTNACC 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 392, 423
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
FaiI YATR 1 cut(s) 499
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 366
Fnu4HI GCNGC 2 cut(s) 99, 330
Fsp4HI GCNGC 2 cut(s) 99, 330
GlaI GCGC 1 cut(s) 127
GluI GCNGC 2 cut(s) 99, 330
GsaI CCCAGC 1 cut(s) 190
HaeIII GGCC 5 cut(s) 134, 173, 185, 215, 398
HapII CCGG 1 cut(s) 240
HgaI GACGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 128
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 2 cut(s) 6, 303
HindII GTYRAC 2 cut(s) 6, 303
HinfI GANTC 1 cut(s) 138
HpaII CCGG 1 cut(s) 240
HphI GGTGA 3 cut(s) 107, 229, 406
Hpy166II GTNNAC 5 cut(s) 6, 49, 148, 303, 346
Hpy188III TCNNGA 1 cut(s) 371
Hpy8I GTNNAC 5 cut(s) 6, 49, 148, 303, 346
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 2 cut(s) 163, 319
HpyCH4III ACNGT 2 cut(s) 46, 307
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 3 cut(s) 32, 107, 316
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 212, 418, 494
HpyF3I CTNAG 2 cut(s) 155, 294
HpySE526I ACGT 1 cut(s) 445
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 5 cut(s) 10, 175, 196, 309, 373
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 9 cut(s) 148, 197, 200, 253, 281, 379, 406, 410, 437
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 437
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 1 cut(s) 235
MalI GATC 5 cut(s) 12, 177, 198, 311, 375
MboI GATC 5 cut(s) 10, 175, 196, 309, 373
MboII GAAGA 5 cut(s) 368, 400, 448, 463, 466
MflI RGATCY 1 cut(s) 196
MlsI TGGCCA 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 134
Mox20I TGGCCA 1 cut(s) 134
MscI TGGCCA 1 cut(s) 134
MseI TTAA 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 134
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 394, 425
MvaI CCWGG 2 cut(s) 394, 425
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 4 cut(s) 104, 212, 418, 494
NdeII GATC 5 cut(s) 10, 175, 196, 309, 373
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 1 cut(s) 235
PfeI GAWTC 1 cut(s) 138
PinAI ACCGGT 1 cut(s) 239
PkrI GCNGC 2 cut(s) 100, 331
Psp6I CCWGG 2 cut(s) 392, 423
PspEI GGTNACC 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 2 cut(s) 392, 423
PspPI GGNCC 3 cut(s) 184, 214, 396
PsuI RGATCY 1 cut(s) 196
RsaI GTAC 3 cut(s) 147, 347, 467
RsaNI GTAC 3 cut(s) 146, 346, 466
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 2 cut(s) 99, 330
Sau3AI GATC 5 cut(s) 10, 175, 196, 309, 373
Sau96I GGNCC 3 cut(s) 184, 214, 396
ScrFI CCNGG 2 cut(s) 394, 425
SfaNI GCATC 1 cut(s) 437
SgrAI CRCCGGYG 1 cut(s) 239
SmlI CTYRAG 3 cut(s) 191, 200, 475
SmoI CTYRAG 3 cut(s) 191, 200, 475
SsiI CCGC 3 cut(s) 99, 206, 223
StyD4I CCNGG 2 cut(s) 392, 423
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 2 cut(s) 46, 307
TaiI ACGT 1 cut(s) 448
TaqI TCGA 1 cut(s) 167
TaqII GACCGA 2 cut(s) 67, 77
TatI WGTACW 2 cut(s) 345, 465
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 1 cut(s) 138
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 2 cut(s) 39, 312
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 2 cut(s) 39, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.