Rmu_sc0005870.1_g000006

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005870.1
OG0003892
Physical Location & Seq
Forward (+)
22379 .. 23032
654 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
atggaactgttacctgatcaaaggagggctcgcaagaagatgtatcgagatcgaaagatgaatatatttaagaagggggaggagctatccatactttgtggtattgatgtcgctgtgattctgttccaaccagctgacaagagtactactgctcaaccagctgcagagacttggcctaaagatcctacagaaatcaagcgcattatccgcagatacaaggcaaagcctacctccagtcatgcttcatcttctagcacaagcccgccggtaatcaaagatactactagtactccatcttcatctccatgtccatgtccatctccatctcaaagtctactagggaagagaaattacttaaacttggatttgtctttgtctggtttttatgactcgcgaatgaaaattttaaaagaggacaaaggtaagaagaaagtacgtgctgataataatgagaatataaattccgaagaggggatgggtatgcagtatgagtttcagacaagatggaatgctgctggaaaagatgaattatacaaggacgacaacgaattgaggattggatatgatgatcatgaggcaaagcttgcttcactggaagctaagcgagaagctataacaaagaggattgcttcattggaggcgaattatgtttaa

Protein Analysis

217

Amino Acids

24.55

Weight (kDa)

9.19

Isoelectric Point (pI)

60.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 8 - 50 3.1e-11 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 334
AccII CGCG 1 cut(s) 394
AciI CCGC 2 cut(s) 208, 263
AclWI GGATC 1 cut(s) 176
AcsI RAATTY 2 cut(s) 402, 460
AfaI GTAC 3 cut(s) 145, 289, 435
AfiI CCNNNNNNNGG 1 cut(s) 471
AhlI ACTAGT 1 cut(s) 284
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 6 cut(s) 85, 134, 161, 583, 599, 611
AluI AGCT 6 cut(s) 85, 134, 161, 583, 599, 611
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 176
AoxI GGCC 1 cut(s) 173
ApeKI GCWGC 2 cut(s) 161, 512
ApoI RAATTY 2 cut(s) 402, 460
AspLEI GCGC 1 cut(s) 201
BanII GRGCYC 1 cut(s) 31
BarI GAAGNNNNNNTAC 4 cut(s) 280, 312, 335, 367
BbvI GCAGC 2 cut(s) 148, 499
BccI CCATC 5 cut(s) 301, 325, 331, 469, 498
BclI TGATCA 2 cut(s) 16, 568
BcoDI GTCTC 1 cut(s) 161
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 3 cut(s) 252, 285, 338
BfmI CTRYAG 2 cut(s) 162, 186
BisI GCNGC 2 cut(s) 162, 513
BlpI GCTNAGC 1 cut(s) 600
BlsI GCNGC 2 cut(s) 163, 514
BmcAI AGTACT 2 cut(s) 145, 289
BpmI CTGGAG 1 cut(s) 217
Bpu1102I GCTNAGC 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 1 cut(s) 471
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 2 cut(s) 234, 597
BseGI GGATG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 471
BseNI ACTGG 2 cut(s) 234, 597
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 2 cut(s) 148, 499
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 1 cut(s) 175
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 471
BsmAI GTCTC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 4 cut(s) 16, 49, 181, 568
Bsp1720I GCTNAGC 1 cut(s) 600
Bsp68I TCGCGA 1 cut(s) 394
BspACI CCGC 2 cut(s) 208, 263
BspANI GGCC 1 cut(s) 175
BspFNI CGCG 1 cut(s) 394
BspHI TCATGA 1 cut(s) 571
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 2 cut(s) 234, 597
BssAI RCCGGY 1 cut(s) 265
BssMI GATC 4 cut(s) 16, 49, 181, 568
Bst4CI ACNGT 1 cut(s) 9
Bst6I CTCTTC 2 cut(s) 338, 462
BstAPI GCANNNNNTGC 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 31, 263, 585
BstDEI CTNAG 1 cut(s) 600
BstF5I GGATG 1 cut(s) 480
BstFNI CGCG 1 cut(s) 394
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 4 cut(s) 19, 52, 184, 571
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 4 cut(s) 16, 49, 181, 568
BstMWI GCNNNNNNNGC 3 cut(s) 158, 207, 584
BstSFI CTRYAG 2 cut(s) 162, 186
BstUI CGCG 1 cut(s) 394
BstV1I GCAGC 2 cut(s) 148, 499
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 480
BtsIMutI CAGTG 1 cut(s) 590
BtuMI TCGCGA 1 cut(s) 394
Cac8I GCNNGC 3 cut(s) 31, 263, 585
CciI TCATGA 1 cut(s) 571
CfoI GCGC 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 265
Csp6I GTAC 3 cut(s) 144, 288, 434
CviAII CATG 4 cut(s) 239, 306, 312, 572
CviQI GTAC 3 cut(s) 144, 288, 434
DdeI CTNAG 1 cut(s) 600
DpnI GATC 4 cut(s) 18, 51, 183, 570
DpnII GATC 4 cut(s) 16, 49, 181, 568
DraI TTTAAA 1 cut(s) 408
Eam1104I CTCTTC 2 cut(s) 338, 462
EarI CTCTTC 2 cut(s) 338, 462
Eco24I GRGCYC 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 31
FaeI CATG 4 cut(s) 242, 309, 315, 575
FatI CATG 4 cut(s) 238, 305, 311, 571
FauI CCCGC 1 cut(s) 270
FbaI TGATCA 2 cut(s) 16, 568
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 162, 513
FokI GGATG 1 cut(s) 487
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 162, 513
FspBI CTAG 3 cut(s) 252, 285, 338
GlaI GCGC 1 cut(s) 200
GluI GCNGC 2 cut(s) 162, 513
GsuI CTGGAG 1 cut(s) 217
HaeIII GGCC 1 cut(s) 175
HapII CCGG 1 cut(s) 266
HhaI GCGC 1 cut(s) 201
Hin1II CATG 4 cut(s) 242, 309, 315, 575
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 2 cut(s) 118, 389
HpaII CCGG 1 cut(s) 266
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 2 cut(s) 466, 498
Hpy188III TCNNGA 3 cut(s) 47, 393, 572
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 2 cut(s) 164, 484
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 207, 584
HpyF3I CTNAG 1 cut(s) 600
HpySE526I ACGT 1 cut(s) 436
Hsp92II CATG 4 cut(s) 242, 309, 315, 575
HspAI GCGC 1 cut(s) 199
Ksp22I TGATCA 2 cut(s) 16, 568
Kzo9I GATC 4 cut(s) 16, 49, 181, 568
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 8 cut(s) 27, 144, 171, 247, 279, 363, 501, 578
Lsp1109I GCAGC 2 cut(s) 148, 499
MaeI CTAG 3 cut(s) 252, 285, 338
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 1 cut(s) 9
MalI GATC 4 cut(s) 18, 51, 183, 570
MboI GATC 4 cut(s) 16, 49, 181, 568
MboII GAAGA 6 cut(s) 49, 240, 288, 355, 439, 479
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 6 cut(s) 349, 402, 460, 527, 548, 643
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 9 cut(s) 18, 73, 241, 406, 463, 546, 568, 615, 631
MseI TTAA 4 cut(s) 69, 356, 407, 652
MslI CAYNNNNRTG 2 cut(s) 304, 310
MspA1I CMGCKG 2 cut(s) 134, 161
MspI CCGG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 514
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 3 cut(s) 158, 207, 584
NdeII GATC 4 cut(s) 16, 49, 181, 568
NlaIII CATG 4 cut(s) 242, 309, 315, 575
NruI TCGCGA 1 cut(s) 394
PagI TCATGA 1 cut(s) 571
PctI GAATGC 1 cut(s) 514
PfeI GAWTC 1 cut(s) 118
PkrI GCNGC 2 cut(s) 163, 514
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
Ppu21I YACGTR 1 cut(s) 437
PstI CTGCAG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 181
PvuII CAGCTG 2 cut(s) 134, 161
RruI TCGCGA 1 cut(s) 394
RsaI GTAC 3 cut(s) 145, 289, 435
RsaNI GTAC 3 cut(s) 144, 288, 434
RseI CAYNNNNRTG 2 cut(s) 304, 310
SaqAI TTAA 4 cut(s) 69, 356, 407, 652
SatI GCNGC 2 cut(s) 162, 513
Sau3AI GATC 4 cut(s) 16, 49, 181, 568
ScaI AGTACT 2 cut(s) 145, 289
SchI GAGTC 1 cut(s) 383
SduI GDGCHC 1 cut(s) 31
SfcI CTRYAG 2 cut(s) 162, 186
SmiMI CAYNNNNRTG 2 cut(s) 304, 310
SpeI ACTAGT 1 cut(s) 284
Sse9I AATT 6 cut(s) 349, 402, 460, 527, 548, 643
SsiI CCGC 2 cut(s) 208, 263
SspMI CTAG 3 cut(s) 252, 285, 338
TaaI ACNGT 1 cut(s) 9
TaiI ACGT 1 cut(s) 439
TaqI TCGA 2 cut(s) 46, 52
TasI AATT 6 cut(s) 349, 402, 460, 527, 548, 643
TatI WGTACW 2 cut(s) 143, 287
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 4 cut(s) 69, 356, 407, 652
Tru9I TTAA 4 cut(s) 69, 356, 407, 652
TscAI CASTG 1 cut(s) 597
TseI GCWGC 2 cut(s) 161, 512
TspDTI ATGAA 6 cut(s) 74, 234, 288, 413, 540, 621
TspRI CASTG 1 cut(s) 597
XapI RAATTY 2 cut(s) 402, 460
XmiI GTMKAC 1 cut(s) 334
XspI CTAG 3 cut(s) 252, 285, 338
ZrmI AGTACT 2 cut(s) 145, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.