Rmu_sc0006228.1_g000001

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006228.1
OG0004242
Physical Location & Seq
Reverse (-)
1483 .. 4593
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3111 bp
atgcctattgttcgagcacttgaatcttcttctgtaagcatcctccttaacaagttggccactaaagatgtcatagatttcttcctcaagtggaaaattgatgactccctgcgagaaaagctcaagagaaacctgcttgttattcatgcagttctcaatcatgctgaggagaagcaagtccgggattcaaatgtcaaagcttggctggaggacgtcagagttgcagcttatgatgctgaggacatattggatgacatcgccaatgatgctctccaatcacaaaacagcaaacatgaggtatggaattctaagatcaaagaagggatagactttaagagcaaagacgttgctaatactcttaatccttttagagaacgtgtagagtcgaagatgataaagattatagatatattggaagagattgcaaaacagaaagatatacttcgtttgagagaagattctggcggtatagaatcaggttttgacagattggccacgactccaatgttgggtgatgaatctcatgtttatggtagagaatctgataaggaggagataattactttgctgaatttgggtggaaatgatgatggggtttctataatcccagttgtaggcatgggaggtattgggaagactacacttgctcagcttgtgtataatgatgctaatgtgagtagccattttgatttgagagcctgggcttgtgtctctgatgtatttgatgtgcaaaggataacaaaaacacttgttgagtctgtgactaaatcaagttgtagcacaaacaatttggagttgcttcaagaacaactgaaaatacggttgaaggacaagaaatttctctttgttctggatgatgtctggaacgagaagtatgaaagctgggatcagttaagagtccccttcacagttggtgcaccagggagtaaaatcatgatcacgacacgaaacaaaacagttgcatcacttatggggactgttcctacttactgtttggaggggctttcggacagtgattgttggttattattcgaacaaattgtgtttaagaacaggaatctagatgcctatccaaagttgaaagtgattggcaggaaaatagcagataagtgtaaagggttgcctttagctgcaaaggcactaggaggtctcctgcgtgctgaaccagaactagatgagaattactggaatgatatcttgaatagcaaactttggaaactggcagacaacactgttctaccagctctaagattaagctaccatcacctccctgggcatttgaagcgctgttttgcttattgctcgatgtttcctaaggactatgaatatgagagggagatgttaatcttgttatggatggcagagggttttgtgccagagccagaaggaaatcaacgaatcgaagatgtagctggaagatatgtttcagattttttatcaaggtccttcttccaacacaccagtgacgagtcacgactggtaatgcatgacctcatacatgatcttgcacaatctgtgtctggcaaaacatttgttaggttggaggaaaattcagaggaccataaaaggtctacaaaagctcggcacttgtcatatacccgtggatctgaagatgtatttcagaaatttgaggcattcagtgaggtggattgtttacgaacctttctaccactagacccattgcgaggattcaatatgagtagcttaactgacaaggttccttgtgatatgatgccaaaattaagattcctgcgggtgctatcctttagtggttatctcataaaaaagtttccagattcaattggcaacttgaagcatctgcgatacctcaatctttctcattgtcaaatagaagagctacctgagccaacaagcagtctatacaatctgcagacattaattttattcagttgtcgatcgcttaccatgctgcctgcagacatgggaaacctaaggaatttacagcatctcaatattcagggaactaggctgaaaagtatgcctttgggaatgggaagattggttaaccttcaaatactgtcagattttattgtggggaaagccatgggaaaagggattgcagaattgaaggacttgtcacatcttcgaggctcagtttctattttgtgtttgcatgatgtcggcagtgttagggatgcaattgaggccagattagaggttaagaagcaccttgatgatttagttttggaatggagcagcagtactgatggttcgcgaaatgaagagatcgagacagaggtacttgatgcactgcagcctcatgaaaacctgaagaaactcaccattaagtattatggaggtacagaatttccatattggatggggaatcctttgttcactaacatggcgtacttgcatctctatggttgcataaaatgcacatctttaccaccccttggccaactaccttctcttaaagatctcataattgaagcaatgcatggaatacagcgtgtaggaatggatttttttggggatgatgatggttccacaatttttttcccctctttagagactttaaaatttgaggacatgaaagaatgggagaaatggtcatcttcagaagatgaaaggcttaaaggatttcccagccttcgggagctttccatatttagatgtcccaagttaacaaagtttacacatggatttgcttctctagaaaagctgcgcatcaagaactgtagagcaataactaccttctcacagaatcctgcacttgggaatttggaacctgtagaattccctagcctgcaactgcttgttcttgttggttgcagtgagcttgaaaaccttcctttttccctccctttgttgaaagtgcttgagatagaaggttgtgaaaaattggctgcacttccgaggatggtggaacttagtagattgtacctgatcgattcaaatgctgaattacttggacggaagatggaatttagctcactgacctctttgcacctaactcatattccacatgttaagcatctacctgaaggctttatccaacagtctgcaaaacttgaagaattgagtcttacaggctttcctgaccttgaatatttggcagatgaccgagttggaatatttggcagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1036

Amino Acids

117.58

Weight (kDa)

5.79

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 99 2.3e-17 Rx N-terminal domain
NB-ARC PF00931 180 - 352 8.2e-38 NB-ARC domain
WHD_DRP PF23559 436 - 503 8.2e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 577 - 850 5.6e-31 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 820 4.4e-40 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 2690
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 2417
AccI GTMKAC 1 cut(s) 1571
AccII CGCG 1 cut(s) 2236
AciI CCGC 2 cut(s) 465, 1753
AclWI GGATC 2 cut(s) 894, 1612
AcoI YGGCCR 3 cut(s) 57, 492, 2419
AcuI CTGAAG 4 cut(s) 1629, 2312, 2565, 3027
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 5 cut(s) 2224, 2262, 2323, 2372, 2906
AfeI AGCGCT 1 cut(s) 1286
AfiI CCNNNNNNNGG 6 cut(s) 509, 614, 1685, 2417, 2616, 2738
AflIII ACRYGT 2 cut(s) 376, 2989
AjnI CCWGG 3 cut(s) 698, 919, 1270
AleI CACNNNNGTG 1 cut(s) 1461
Alw21I GWGCWC 2 cut(s) 19, 919
Alw26I GTCTC 4 cut(s) 715, 1154, 2246, 2528
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 2 cut(s) 894, 1612
Aor51HI AGCGCT 1 cut(s) 1286
AoxI GGCC 4 cut(s) 57, 492, 2166, 2419
ApaLI GTGCAC 1 cut(s) 915
ApeKI GCWGC 7 cut(s) 224, 1130, 1930, 2217, 2275, 2686, 2870
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 1 cut(s) 2811
AspLEI GCGC 2 cut(s) 1287, 2691
AspS9I GGNCC 2 cut(s) 1443, 1558
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 3 cut(s) 524, 1256, 2293
AsuII TTCGAA 1 cut(s) 1032
AvaII GGWCC 2 cut(s) 1443, 1558
AxyI CCTNAGG 2 cut(s) 1314, 1952
BaeGI GKGCMC 1 cut(s) 919
BalI TGGCCA 3 cut(s) 59, 494, 2421
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 2 cut(s) 19, 919
BbvCI CCTCAGC 2 cut(s) 165, 237
BbvI GCAGC 7 cut(s) 236, 1117, 1917, 2229, 2287, 2673, 2857
BccI CCATC 8 cut(s) 584, 1269, 1351, 2222, 2335, 2498, 2878, 2938
BcgI CGANNNNNNTGC 2 cut(s) 2903, 2937
BciT130I CCWGG 3 cut(s) 700, 921, 1272
BclI TGATCA 1 cut(s) 936
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 4 cut(s) 715, 1154, 2246, 2528
BfaI CTAG 7 cut(s) 1061, 1142, 1172, 1673, 1986, 2678, 2766
BfmI CTRYAG 5 cut(s) 1889, 1935, 2273, 2701, 2754
BfoI RGCGCY 1 cut(s) 1288
BfuAI ACCTGC 1 cut(s) 141
BglII AGATCT 1 cut(s) 2440
BisI GCNGC 7 cut(s) 225, 1131, 1931, 2218, 2276, 2687, 2871
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 7 cut(s) 226, 1132, 1932, 2219, 2277, 2688, 2872
BmcAI AGTACT 1 cut(s) 2224
Bme1390I CCNGG 4 cut(s) 182, 700, 921, 1272
Bme18I GGWCC 2 cut(s) 1443, 1558
BmgT120I GGNCC 2 cut(s) 1443, 1558
BmiI GGNNCC 3 cut(s) 1719, 2509, 2751
BmrFI CCNGG 4 cut(s) 182, 700, 921, 1272
BmrI ACTGGG 1 cut(s) 602
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 1 cut(s) 641
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 3 cut(s) 165, 237, 1863
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 1032
BpuEI CTTGAG 3 cut(s) 71, 107, 2864
BpuMI CCSGG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 2913
BsaBI GATNNNNATC 2 cut(s) 1068, 1343
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 1154
BsaJI CCNNGG 8 cut(s) 699, 920, 1270, 1271, 1600, 2064, 2416, 2879
BsaXI ACNNNNNCTCC 4 cut(s) 2206, 2236, 2558, 2588
Bsc4I CCNNNNNNNGG 6 cut(s) 509, 614, 1685, 2417, 2616, 2738
Bse1I ACTGG 5 cut(s) 608, 1190, 1224, 1461, 1482
Bse21I CCTNAGG 2 cut(s) 1314, 1952
Bse3DI GCAATG 2 cut(s) 1679, 2463
Bse8I GATNNNNATC 2 cut(s) 1068, 1343
BseBI CCWGG 3 cut(s) 700, 921, 1272
BseCI ATCGAT 1 cut(s) 2913
BseDI CCNNGG 8 cut(s) 699, 920, 1270, 1271, 1600, 2064, 2416, 2879
BseGI GGATG 8 cut(s) 39, 256, 859, 1362, 2161, 2346, 2503, 2889
BseJI GATNNNNATC 2 cut(s) 1068, 1343
BseLI CCNNNNNNNGG 6 cut(s) 509, 614, 1685, 2417, 2616, 2738
BseMI GCAATG 2 cut(s) 1679, 2463
BseMII CTCAG 5 cut(s) 156, 228, 662, 1854, 2127
BseNI ACTGG 5 cut(s) 608, 1190, 1224, 1461, 1482
BseRI GAGGAG 2 cut(s) 182, 566
BseSI GKGCMC 1 cut(s) 919
BseXI GCAGC 7 cut(s) 236, 1117, 1917, 2229, 2287, 2673, 2857
BseYI CCCAGC 2 cut(s) 882, 2609
BsgI GTGCAG 2 cut(s) 2718, 2856
Bsh1236I CGCG 1 cut(s) 2236
Bsh1285I CGRYCG 1 cut(s) 1919
BshFI GGCC 4 cut(s) 59, 494, 2168, 2421
BshVI ATCGAT 1 cut(s) 2913
BsiEI CGRYCG 1 cut(s) 1919
BsiHKAI GWGCWC 2 cut(s) 19, 919
BsiSI CCGG 1 cut(s) 181
BslFI GGGAC 3 cut(s) 884, 988, 2625
BslI CCNNNNNNNGG 6 cut(s) 509, 614, 1685, 2417, 2616, 2738
BsmAI GTCTC 4 cut(s) 715, 1154, 2246, 2528
BsmFI GGGAC 3 cut(s) 884, 988, 2625
BsmI GAATGC 1 cut(s) 1634
BsnI GGCC 4 cut(s) 59, 494, 2168, 2421
Bso31I GGTCTC 1 cut(s) 1154
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 2 cut(s) 19, 919
Bsp143I GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 2064
Bsp68I TCGCGA 1 cut(s) 2236
BspACI CCGC 2 cut(s) 465, 1753
BspANI GGCC 4 cut(s) 59, 494, 2168, 2421
BspCNI CTCAG 5 cut(s) 157, 229, 661, 1855, 2126
BspDI ATCGAT 1 cut(s) 2913
BspFNI CGCG 1 cut(s) 2236
BspHI TCATGA 2 cut(s) 933, 2281
BspLI GGNNCC 3 cut(s) 1719, 2509, 2751
BspMAI CTGCAG 3 cut(s) 1893, 1939, 2277
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 894, 1612
BspQI GCTCTTC 1 cut(s) 1848
BspT104I TTCGAA 1 cut(s) 1032
BspTNI GGTCTC 1 cut(s) 1154
BsrDI GCAATG 2 cut(s) 1679, 2463
BsrI ACTGG 5 cut(s) 608, 1190, 1224, 1461, 1482
BssECI CCNNGG 8 cut(s) 699, 920, 1270, 1271, 1600, 2064, 2416, 2879
BssMI GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 2064, 2416
Bst2UI CCWGG 3 cut(s) 700, 921, 1272
Bst6I CTCTTC 3 cut(s) 411, 1848, 2238
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 2397
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 3 cut(s) 1158, 1935, 2771
BstDSI CCRYGG 2 cut(s) 1600, 2064
BstF5I GGATG 8 cut(s) 39, 256, 859, 1362, 2161, 2346, 2503, 2889
BstFNI CGCG 1 cut(s) 2236
BstH2I RGCGCY 1 cut(s) 1288
BstHHI GCGC 2 cut(s) 1287, 2691
BstKTI GATC 9 cut(s) 315, 889, 939, 1504, 1607, 1919, 2250, 2443, 2913
BstMAI GTCTC 4 cut(s) 715, 1154, 2246, 2528
BstMBI GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
BstMCI CGRYCG 1 cut(s) 1919
BstMWI GCNNNNNNNGC 9 cut(s) 118, 233, 266, 1136, 1282, 1864, 1927, 2165, 2397
BstNI CCWGG 3 cut(s) 700, 921, 1272
BstNSI RCATGY 1 cut(s) 2993
BstSCI CCNGG 4 cut(s) 180, 698, 919, 1270
BstSFI CTRYAG 5 cut(s) 1889, 1935, 2273, 2701, 2754
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 1 cut(s) 2236
BstV1I GCAGC 7 cut(s) 236, 1117, 1917, 2229, 2287, 2673, 2857
BstV2I GAAGAC 1 cut(s) 641
BstX2I RGATCY 2 cut(s) 1604, 2440
BstYI RGATCY 2 cut(s) 1604, 2440
Bsu15I ATCGAT 1 cut(s) 2913
Bsu36I CCTNAGG 2 cut(s) 1314, 1952
BsuRI GGCC 4 cut(s) 59, 494, 2168, 2421
BsuTUI ATCGAT 1 cut(s) 2913
BtgI CCRYGG 2 cut(s) 1600, 2064
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 8 cut(s) 39, 256, 859, 1362, 2161, 2346, 2503, 2889
BtsI GCAGTG 3 cut(s) 2152, 2270, 2803
BtsIMutI CAGTG 8 cut(s) 1018, 1230, 1468, 1645, 2152, 2270, 2803, 2957
BtuMI TCGCGA 1 cut(s) 2236
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 3 cut(s) 1158, 1935, 2771
CciI TCATGA 2 cut(s) 933, 2281
CfoI GCGC 2 cut(s) 1287, 2691
Cfr13I GGNCC 2 cut(s) 1443, 1558
ClaI ATCGAT 1 cut(s) 2913
Csp6I GTAC 5 cut(s) 2223, 2261, 2322, 2371, 2905
CviQI GTAC 5 cut(s) 2223, 2261, 2322, 2371, 2905
DpnI GATC 9 cut(s) 314, 888, 938, 1503, 1606, 1918, 2249, 2442, 2912
DpnII GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
DraI TTTAAA 1 cut(s) 2541
EaeI YGGCCR 3 cut(s) 57, 492, 2419
Eam1104I CTCTTC 3 cut(s) 411, 1848, 2238
EarI CTCTTC 3 cut(s) 411, 1848, 2238
Eco130I CCWWGG 2 cut(s) 2064, 2416
Eco31I GGTCTC 1 cut(s) 1154
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 2 cut(s) 1443, 1558
Eco47III AGCGCT 1 cut(s) 1286
Eco57I CTGAAG 4 cut(s) 1629, 2312, 2565, 3027
Eco81I CCTNAGG 2 cut(s) 1314, 1952
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 2 cut(s) 304, 2759
EcoRII CCWGG 3 cut(s) 698, 919, 1270
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 2 cut(s) 2064, 2416
EcoT22I ATGCAT 2 cut(s) 1488, 2463
ErhI CCWWGG 2 cut(s) 2064, 2416
FalI AAGNNNNNCTT 4 cut(s) 426, 458, 1987, 2019
FaqI GGGAC 3 cut(s) 884, 988, 2625
FauI CCCGC 1 cut(s) 1746
FbaI TGATCA 1 cut(s) 936
FblI GTMKAC 1 cut(s) 1571
Fnu4HI GCNGC 7 cut(s) 225, 1131, 1931, 2218, 2276, 2687, 2871
FokI GGATG 8 cut(s) 26, 263, 866, 1369, 2168, 2353, 2510, 2896
Fsp4HI GCNGC 7 cut(s) 225, 1131, 1931, 2218, 2276, 2687, 2871
FspBI CTAG 7 cut(s) 1061, 1142, 1172, 1673, 1986, 2678, 2766
FspI TGCGCA 1 cut(s) 2690
GlaI GCGC 2 cut(s) 1286, 2690
GluI GCNGC 7 cut(s) 225, 1131, 1931, 2218, 2276, 2687, 2871
GsaI CCCAGC 2 cut(s) 886, 2613
GsuI CTGGAG 1 cut(s) 227
HaeII RGCGCY 1 cut(s) 1288
HaeIII GGCC 4 cut(s) 59, 494, 2168, 2421
HapII CCGG 1 cut(s) 181
HhaI GCGC 2 cut(s) 1287, 2691
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 2 cut(s) 1285, 2689
HinP1I GCGC 2 cut(s) 1285, 2689
HincII GTYRAC 2 cut(s) 2026, 2649
HindII GTYRAC 2 cut(s) 2026, 2649
HindIII AAGCTT 1 cut(s) 198
HpaI GTTAAC 2 cut(s) 2026, 2649
HpaII CCGG 1 cut(s) 181
HphI GGTGA 3 cut(s) 524, 1256, 2293
Hpy166II GTNNAC 7 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658
Hpy8I GTNNAC 7 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658
HpyCH4IV ACGT 3 cut(s) 213, 345, 376
HpyF10VI GCNNNNNNNGC 9 cut(s) 118, 233, 266, 1136, 1282, 1864, 1927, 2165, 2397
HpySE526I ACGT 3 cut(s) 213, 345, 376
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 2 cut(s) 1285, 2689
Ksp22I TGATCA 1 cut(s) 936
KspAI GTTAAC 2 cut(s) 2026, 2649
Kzo9I GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
LguI GCTCTTC 1 cut(s) 1848
LmnI GCTCC 2 cut(s) 2214, 2620
Lsp1109I GCAGC 7 cut(s) 236, 1117, 1917, 2229, 2287, 2673, 2857
MaeI CTAG 7 cut(s) 1061, 1142, 1172, 1673, 1986, 2678, 2766
MaeII ACGT 3 cut(s) 213, 345, 376
MaeIII GTNAC 4 cut(s) 760, 1463, 1470, 2097
MalI GATC 9 cut(s) 314, 888, 938, 1503, 1606, 1918, 2249, 2442, 2912
MboI GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
MfeI CAATTG 2 cut(s) 1800, 2160
MflI RGATCY 2 cut(s) 1604, 2440
MhlI GDGCHC 2 cut(s) 19, 919
MlsI TGGCCA 3 cut(s) 59, 494, 2421
MluNI TGGCCA 3 cut(s) 59, 494, 2421
MlyI GAGTC 7 cut(s) 98, 392, 493, 764, 906, 1478, 3055
MmeI TCCRAC 4 cut(s) 1477, 1521, 3043, 3073
Mox20I TGGCCA 3 cut(s) 59, 494, 2421
Mph1103I ATGCAT 2 cut(s) 1488, 2463
MroXI GAANNNNTTC 1 cut(s) 2811
MscI TGGCCA 3 cut(s) 59, 494, 2421
MslI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
Msp20I TGGCCA 3 cut(s) 59, 494, 2421
MspI CCGG 1 cut(s) 181
MspR9I CCNGG 4 cut(s) 182, 700, 921, 1272
MunI CAATTG 2 cut(s) 1800, 2160
Mva1269I GAATGC 1 cut(s) 1634
MvaI CCWGG 3 cut(s) 700, 921, 1272
MvnI CGCG 1 cut(s) 2236
MwoI GCNNNNNNNGC 9 cut(s) 118, 233, 266, 1136, 1282, 1864, 1927, 2165, 2397
NciI CCSGG 1 cut(s) 182
NcoI CCATGG 1 cut(s) 2064
NdeII GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
NlaIV GGNNCC 3 cut(s) 1719, 2509, 2751
NmeAIII GCCGAG 1 cut(s) 1561
NmuCI GTSAC 4 cut(s) 760, 1463, 1470, 2097
NruI TCGCGA 1 cut(s) 2236
NsbI TGCGCA 1 cut(s) 2690
NsiI ATGCAT 2 cut(s) 1488, 2463
NspI RCATGY 1 cut(s) 2993
NspV TTCGAA 1 cut(s) 1032
OliI CACNNNNGTG 1 cut(s) 1461
PagI TCATGA 2 cut(s) 933, 2281
PasI CCCWGGG 1 cut(s) 1271
PciI ACATGT 1 cut(s) 2989
PciSI GCTCTTC 1 cut(s) 1848
PctI GAATGC 1 cut(s) 1634
PdmI GAANNNNTTC 1 cut(s) 2811
PflMI CCANNNNNTGG 1 cut(s) 2417
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 7 cut(s) 226, 1132, 1932, 2219, 2277, 2688, 2872
Ple19I CGATCG 1 cut(s) 1919
PleI GAGTC 7 cut(s) 98, 391, 493, 763, 905, 1477, 3054
PpsI GAGTC 7 cut(s) 98, 391, 493, 763, 905, 1477, 3054
PpuMI RGGWCCY 1 cut(s) 1443
PscI ACATGT 1 cut(s) 2989
PshBI ATTAAT 1 cut(s) 1898
Psp5II RGGWCCY 1 cut(s) 1443
Psp6I CCWGG 3 cut(s) 698, 919, 1270
PspFI CCCAGC 2 cut(s) 882, 2609
PspGI CCWGG 3 cut(s) 698, 919, 1270
PspN4I GGNNCC 3 cut(s) 1719, 2509, 2751
PspPI GGNCC 2 cut(s) 1443, 1558
PspPPI RGGWCCY 1 cut(s) 1443
PsrI GAACNNNNNNTAC 2 cut(s) 2215, 2247
PstI CTGCAG 3 cut(s) 1893, 1939, 2277
PsuI RGATCY 2 cut(s) 1604, 2440
PvuI CGATCG 1 cut(s) 1919
RruI TCGCGA 1 cut(s) 2236
RsaI GTAC 5 cut(s) 2224, 2262, 2323, 2372, 2906
RsaNI GTAC 5 cut(s) 2223, 2261, 2322, 2371, 2905
RseI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
SapI GCTCTTC 1 cut(s) 1848
SatI GCNGC 7 cut(s) 225, 1131, 1931, 2218, 2276, 2687, 2871
Sau3AI GATC 9 cut(s) 312, 886, 936, 1501, 1604, 1916, 2247, 2440, 2910
Sau96I GGNCC 2 cut(s) 1443, 1558
ScaI AGTACT 1 cut(s) 2224
SchI GAGTC 7 cut(s) 98, 392, 493, 764, 906, 1478, 3055
ScrFI CCNGG 4 cut(s) 182, 700, 921, 1272
SduI GDGCHC 2 cut(s) 19, 919
SfcI CTRYAG 5 cut(s) 1889, 1935, 2273, 2701, 2754
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 2 cut(s) 1443, 1558
SmiMI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
SmlI CTYRAG 3 cut(s) 86, 122, 2843
SmoI CTYRAG 3 cut(s) 86, 122, 2843
SsiI CCGC 2 cut(s) 465, 1753
SspI AATATT 3 cut(s) 1975, 3074, 3099
SspMI CTAG 7 cut(s) 1061, 1142, 1172, 1673, 1986, 2678, 2766
StyD4I CCNGG 4 cut(s) 180, 698, 919, 1270
StyI CCWWGG 2 cut(s) 2064, 2416
TaiI ACGT 3 cut(s) 216, 348, 379
TaqI TCGA 9 cut(s) 13, 386, 1032, 1304, 1401, 1915, 2107, 2250, 2913
TaqII GACCGA 1 cut(s) 3102
TatI WGTACW 1 cut(s) 2222
TscAI CASTG 8 cut(s) 1018, 1237, 1468, 1645, 2152, 2277, 2803, 2964
TseFI GTSAC 4 cut(s) 760, 1463, 1470, 2097
TseI GCWGC 7 cut(s) 224, 1130, 1930, 2217, 2275, 2686, 2870
Tsp45I GTSAC 4 cut(s) 760, 1463, 1470, 2097
TspDTI ATGAA 8 cut(s) 134, 531, 891, 1338, 2256, 2298, 2570, 2604
TspGWI ACGGA 1 cut(s) 2953
TspRI CASTG 8 cut(s) 1018, 1237, 1468, 1645, 2152, 2277, 2803, 2964
Van91I CCANNNNNTGG 1 cut(s) 2417
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 1443, 1558
VspI ATTAAT 1 cut(s) 1898
XbaI TCTAGA 2 cut(s) 1060, 2677
XceI RCATGY 1 cut(s) 2993
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XmiI GTMKAC 1 cut(s) 1571
XmnI GAANNNNTTC 1 cut(s) 2811
XspI CTAG 7 cut(s) 1061, 1142, 1172, 1673, 1986, 2678, 2766
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 2224
Zsp2I ATGCAT 2 cut(s) 1488, 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.