Rmu_ssc0000113.1_g000006

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000113.1
OG0003250
Physical Location & Seq
Forward (+)
25542 .. 32419
6878 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3417 bp
atggcaaaacaggagcaaaagcaggagcagcctgcaatcatcaaccctctgcaggtccaggaagacggggatctggaccctggatctgacgacctcgaggctcctctgcctctcaccgtcacttcccgggtcttgtatatgttgggtgacattgcgtctggaccggccttcaaaatcagtcaatggctggaattggtccgtaaacgcagtgccaaataccattcctctggcttccctcgccgcccattaaggcttgacacaatgcctgctagtgcaggagagtgtagtgaggactggaaaggtcctgcgtcgcctgagcaaaccacagaagtcaatctctgggaaagacttggtaaagctgctacattggacattgagtcgagttttttctcatgggataggctttcttcacttcaccacactgaacatagtagtagcaatgatcagtctgaggatgaaatgaacaaagcgctggaggtcaccgtgaattcggggggagttgttttctttgcccttttcaaccagccagggactgatgataactcttctaaggaagcagcggctgttataaagatatcttcatcgaggatggccacacagtctgaacgtctaggttacgaatttgcaaagtggcttggagttcgaactccacaggccagagtcatacataattgtagtatggagtggctccagatgaaggaagcagcagaaaaagcaagggaagcagcagtttcagaaggagatgaatgtggggaaatgacatgttcggaacttttggaagctcttgaacttagccgatgccttttgctcatgagctatgttcacggatctcctttacttgaaagctcaagtgtatttgaggtgaaggaaactgcagagaaaacagcagcttctcttggtaggatcttgatgttggaccttgtcatcagaaatgaagatagactaccttgccgtcagctcagatggcgtggaaactcggcaaatctattgtttgcagacaaaatggattttggaaatatggacagattggaggaagcctttgattctgccatcaagagatacaaaccaagagtgatcagagctcttcaaaaggatagaagggcaacttcagtagatatcagattgcataatacagcattggtaccacagaaatctgatctttctgacattatggattcgccaaggtctagtcagatgagcgtaaagagtcaactctcagatgatttaatgttttccgattttccagttgtggctattgattctggcgttcctcgtcaacctcctgctggaaaacgtgcaaatgaccaggaaatttatccaaagttggttgaattactactgaacaattctgaatactcctctaatctgttacatgacataacattggggaaattaggacgtcctccgttacaagatactgatgcatctgatacacaagcaatggaaatgactttggttgttcaagggttccgtagtgggttcaaacgtgctctcagggatctgcaaggattccatatcttcttactcacacttcatcaaaaactggagaatttgatacgtatatttttcaatattatagataaaataggggagtccgataaggaggatttggtagttattgagtctccatcacaagctgccggaagtgtagagcctaacagcgacgcatttacattcgttcccatgcacctattttctgtggccaataaagacctacataaccccgggagggtcccgcccctagataaaatctcacacaccctcctacgtggctccatctcccaccctccattactcggactcagtatcccggttggaattatggctagggttttggttcagtccacgaatattcccggtgtggttggcgggcgaaggcttgcccaatctaaaggatctggaaatactagaaggtcgaccaagatgatgtgtaccttgcgagcacctgctttgagactgagcagtttctccggactacgtagtgttaatgctctagatattatggcgaggcctgggcaagacttgtattctaaggtgggagtagctatctcctcccggcgtcaaaaggctagtagatgtgtgcccaaagccatgtttgagcgcttcacagaaaaagcaataaaggtgattatgcttgcacaagaggaagcaaggcgactcggtcacaactttgttggtacagagcagatacttttgggtcttattggtgaaggaactggaattgctgccaaggtccttaaatctatgggaatcaatcttaaagatgcacgcgttgaagtggaaaagatcattggaaggggtagtggctttgttgctgttgaaatcccattcactcctcgtgcaaagcgagttttggaactgtcactggaggaagctcgacaactgggccataactatattggttctgaacacttgcttttgggactgcttcgggaaggtgagggtgtggcagctcgtgttcttgagaatttaggcgctgaccccagcaacattcgtacacaggttattcgtatggtgggtgagagcacagaagctgttggtgttggagctgggcagggaagcagtggcaacaagatgccaactctggaggagtatggtaccaatttgactaagctagcagaggagggaaagctggatcctgttgttggaagacaaccacaaattgaacgtgttgtccaaattttgggtaggcggacaaagaataacccctgcctgattggggaacctggtgttgggaagactgcaattgctgaaggtcttgctcaacgaattgcaagcggtgatgtgcctgaaacaatagagggaaagaaggttatcactctggatatgggtcttcttgttgctggtacaaaataccgtggagagttcgaggaaagattaaagaagctgatggaggaaatcaaacaaagtgatgagataattttgttcattgatgaggtgcacactttaattggagcaggagcagcagaaggtgcaattgatgctgccaatatattaaaaccagctcttgcaagaggtgaactgcagtgtattggagccaccactctagatgaatacagaaagcacattgagaaagacccagcattggaaagacgttttcaaccagttaaagttcctgaaccatctgtggacgaaacaatacagattttgcgagggctccgggagcgatatgagatccaccacaaactccggtatactgatgaagctctagtatctgccgctcagctatcataccagtacatcagtgatcgctttctacctgataaagcaattgacttgattgatgaagctggttctcgagttcgccttcgtcatgcacagctacctgaagaagctagagaccttgacaaagaactgagacagattaccaaggagaagaatgaagctgttcgaagccaagactttgaaaaggtatag

Protein Analysis

1138

Amino Acids

125.8

Weight (kDa)

6.1

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Act-Frag_cataly PF09192 110 - 423 1.5e-46 Actin-fragmin kinase, catalytic
Clp_N PF02861 701 - 823 1.9e-36 Clp repeat (R) N-terminal domain
Clp_N PF02861 777 - 849 1.4e-16 Clp repeat (R) N-terminal domain
AAA PF00004 904 - 1016 8.9e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 1042 - 1137 1e-33 AAA lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
AarI CACCTGC 1 cut(s) 1954
AatII GACGTC 1 cut(s) 1402
AbsI CCTCGAGG 1 cut(s) 95
Acc36I ACCTGC 2 cut(s) 43, 1954
Acc65I GGTACC 2 cut(s) 1141, 2597
AccB1I GGYRCC 2 cut(s) 1141, 2597
AccB7I CCANNNNNTGG 1 cut(s) 2683
AccBSI CCGCTC 1 cut(s) 3221
AccI GTMKAC 2 cut(s) 1916, 3194
AccII CGCG 1 cut(s) 2271
AccIII TCCGGA 1 cut(s) 1970
AciI CCGC 7 cut(s) 241, 560, 1736, 1869, 2692, 2778, 3219
AclWI GGATC 9 cut(s) 78, 91, 835, 911, 1506, 1903, 2630, 2643, 3169
AcoI YGGCCR 2 cut(s) 591, 1701
AcsI RAATTY 6 cut(s) 487, 620, 1311, 1549, 2467, 2679
AcuI CTGAAG 3 cut(s) 1092, 2772, 3348
AcyI GRCGYC 2 cut(s) 1399, 2059
AfaI GTAC 7 cut(s) 1143, 1933, 2179, 2497, 2599, 2848, 3239
AfeI AGCGCT 2 cut(s) 471, 2102
AflIII ACRYGT 3 cut(s) 761, 2269, 2668
AhdI GACNNNNNGTC 2 cut(s) 154, 376
AjnI CCWGG 6 cut(s) 57, 79, 526, 1305, 2011, 2725
AjuI GAANNNNNNNTTGG 4 cut(s) 2274, 2306, 2715, 2747
AloI GAACNNNNNNTCC 2 cut(s) 2658, 2690
Alw21I GWGCWC 5 cut(s) 1084, 1492, 1945, 2528, 2943
Alw26I GTCTC 4 cut(s) 1629, 1948, 3333, 3352
Alw44I GTGCAC 1 cut(s) 2939
AlwI GGATC 9 cut(s) 78, 91, 835, 911, 1506, 1903, 2630, 2643, 3169
AlwNI CAGNNNCTG 3 cut(s) 533, 563, 2534
Ama87I CYCGRG 4 cut(s) 95, 126, 1724, 3297
Aor13HI TCCGGA 1 cut(s) 1970
Aor51HI AGCGCT 2 cut(s) 471, 2102
AoxI GGCC 6 cut(s) 165, 591, 654, 1701, 2009, 2386
ApaLI GTGCAC 1 cut(s) 2939
ApoI RAATTY 6 cut(s) 487, 620, 1311, 1549, 2467, 2679
Asp700I GAANNNNTTC 1 cut(s) 3387
Asp718I GGTACC 2 cut(s) 1141, 2597
AspLEI GCGC 3 cut(s) 472, 2103, 2477
AspS9I GGNCC 9 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233, 2386
AsuC2I CCSGG 8 cut(s) 127, 128, 1725, 1726, 1811, 1857, 2056, 3161
AsuII TTCGAA 2 cut(s) 643, 3391
AsuNHI GCTAGC 1 cut(s) 2614
AvaI CYCGRG 4 cut(s) 95, 126, 1724, 3297
AvaII GGWCC 8 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233
BaeGI GKGCMC 2 cut(s) 2085, 2943
BalI TGGCCA 2 cut(s) 593, 1703
BamHI GGATCC 1 cut(s) 2635
BanI GGYRCC 2 cut(s) 1141, 2597
BanII GRGCYC 2 cut(s) 1084, 3159
BarI GAAGNNNNNNTAC 2 cut(s) 927, 959
BauI CACGAG 2 cut(s) 2337, 2454
BbsI GAAGAC 4 cut(s) 69, 2656, 2744, 2825
Bbv12I GWGCWC 5 cut(s) 1084, 1492, 1945, 2528, 2943
BccI CCATC 7 cut(s) 583, 957, 1058, 1636, 1784, 2884, 3130
BceAI ACGGC 1 cut(s) 936
BciT130I CCWGG 6 cut(s) 59, 81, 528, 1307, 2013, 2727
BciVI GTATCC 1 cut(s) 1817
BclI TGATCA 2 cut(s) 442, 1074
BcnI CCSGG 8 cut(s) 127, 128, 1725, 1726, 1811, 1857, 2056, 3161
BcoDI GTCTC 4 cut(s) 1629, 1948, 3333, 3352
BfmI CTRYAG 3 cut(s) 50, 873, 3023
BfoI RGCGCY 3 cut(s) 473, 2104, 2478
BfuAI ACCTGC 2 cut(s) 43, 1954
BfuI GTATCC 1 cut(s) 1817
BlpI GCTNAGC 1 cut(s) 3222
Bme18I GGWCC 8 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233
BmeRI GACNNNNNGTC 2 cut(s) 154, 376
BmeT110I CYCGRG 4 cut(s) 95, 126, 1724, 3297
BmgT120I GGNCC 9 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233, 2386
BmrI ACTGGG 1 cut(s) 2393
BmsI GCATC 6 cut(s) 788, 1411, 1433, 2254, 2565, 2971
BmtI GCTAGC 1 cut(s) 2618
BmuI ACTGGG 1 cut(s) 2393
BpiI GAAGAC 4 cut(s) 69, 2656, 2744, 2825
BpmI CTGGAG 5 cut(s) 494, 674, 1565, 2387, 2606
Bpu10I CCTNAGC 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 3222
Bpu14I TTCGAA 2 cut(s) 643, 3391
BpuEI CTTGAG 2 cut(s) 832, 2483
BpuMI CCSGG 8 cut(s) 127, 128, 1725, 1726, 1811, 1857, 2056, 3161
BsaAI YACGTR 3 cut(s) 1559, 1769, 1979
BsaHI GRCGYC 2 cut(s) 1399, 2059
BsaI GGTCTC 1 cut(s) 3333
BsaWI WCCGGW 2 cut(s) 1970, 3189
BsaXI ACNNNNNCTCC 2 cut(s) 1746, 1776
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 8 cut(s) 299, 1244, 1548, 2221, 2370, 2388, 3104, 3235
Bse3DI GCAATG 3 cut(s) 150, 445, 1446
BseAI TCCGGA 1 cut(s) 1970
BseBI CCWGG 6 cut(s) 59, 81, 528, 1307, 2013, 2727
BseGI GGATG 2 cut(s) 460, 594
BseMI GCAATG 3 cut(s) 150, 445, 1446
BseMII CTCAG 9 cut(s) 306, 441, 973, 1230, 1507, 1816, 1949, 3236, 3347
BseNI ACTGG 8 cut(s) 299, 1244, 1548, 2221, 2370, 2388, 3104, 3235
BseRI GAGGAG 6 cut(s) 93, 1348, 2041, 2325, 2603, 2636
BseSI GKGCMC 2 cut(s) 2085, 2943
BseYI CCCAGC 3 cut(s) 2483, 2549, 3079
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 2271
BshFI GGCC 6 cut(s) 167, 593, 656, 1703, 2011, 2388
BshNI GGYRCC 2 cut(s) 1141, 2597
BsiHKAI GWGCWC 5 cut(s) 1084, 1492, 1945, 2528, 2943
BsiHKCI CYCGRG 4 cut(s) 95, 126, 1724, 3297
BslFI GGGAC 3 cut(s) 544, 1718, 2436
BsmAI GTCTC 4 cut(s) 1629, 1948, 3333, 3352
BsmFI GGGAC 3 cut(s) 544, 1718, 2436
BsnI GGCC 6 cut(s) 167, 593, 656, 1703, 2011, 2388
Bso31I GGTCTC 1 cut(s) 3333
BsoBI CYCGRG 4 cut(s) 95, 126, 1724, 3297
Bsp119I TTCGAA 2 cut(s) 643, 3391
Bsp1286I GDGCHC 7 cut(s) 1084, 1492, 1945, 2085, 2528, 2943, 3159
Bsp13I TCCGGA 1 cut(s) 1970
Bsp1720I GCTNAGC 1 cut(s) 3222
BspACI CCGC 7 cut(s) 241, 560, 1736, 1869, 2692, 2778, 3219
BspANI GGCC 6 cut(s) 167, 593, 656, 1703, 2011, 2388
BspCNI CTCAG 9 cut(s) 307, 442, 972, 1229, 1506, 1815, 1950, 3235, 3348
BspEI TCCGGA 1 cut(s) 1970
BspFNI CGCG 1 cut(s) 2271
BspHI TCATGA 1 cut(s) 810
BspMAI CTGCAG 3 cut(s) 54, 877, 3027
BspMI ACCTGC 2 cut(s) 43, 1954
BspOI GCTAGC 1 cut(s) 2618
BspPI GGATC 9 cut(s) 78, 91, 835, 911, 1506, 1903, 2630, 2643, 3169
BspQI GCTCTTC 1 cut(s) 1089
BspT104I TTCGAA 2 cut(s) 643, 3391
BspT107I GGYRCC 2 cut(s) 1141, 2597
BspTNI GGTCTC 1 cut(s) 3333
BsrBI CCGCTC 1 cut(s) 3221
BsrDI GCAATG 3 cut(s) 150, 445, 1446
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 8 cut(s) 299, 1244, 1548, 2221, 2370, 2388, 3104, 3235
BssAI RCCGGY 1 cut(s) 163
BssNAI GTATAC 1 cut(s) 3195
BssNI GRCGYC 2 cut(s) 1399, 2059
BssSI CACGAG 2 cut(s) 2337, 2454
BssT1I CCWWGG 3 cut(s) 1181, 2229, 3369
Bst1107I GTATAC 1 cut(s) 3195
Bst2BI CACGAG 2 cut(s) 2337, 2454
Bst2UI CCWGG 6 cut(s) 59, 81, 528, 1307, 2013, 2727
Bst4CI ACNGT 5 cut(s) 118, 484, 600, 2361, 2858
Bst6I CTCTTC 2 cut(s) 550, 1089
BstACI GRCGYC 2 cut(s) 1399, 2059
BstAPI GCANNNNNTGC 2 cut(s) 2972, 2981
BstBAI YACGTR 3 cut(s) 1559, 1769, 1979
BstBI TTCGAA 2 cut(s) 643, 3391
BstC8I GCNNGC 9 cut(s) 33, 267, 1871, 1881, 1941, 2136, 2269, 2616, 2776
BstDSI CCRYGG 1 cut(s) 2857
BstEII GGTNACC 1 cut(s) 478
BstF5I GGATG 2 cut(s) 460, 594
BstFNI CGCG 1 cut(s) 2271
BstH2I RGCGCY 3 cut(s) 473, 2104, 2478
BstHHI GCGC 3 cut(s) 472, 2103, 2477
BstMAI GTCTC 4 cut(s) 1629, 1948, 3333, 3352
BstMWI GCNNNNNNNGC 9 cut(s) 28, 237, 713, 722, 964, 2963, 2972, 2981, 3163
BstNI CCWGG 6 cut(s) 59, 81, 528, 1307, 2013, 2727
BstNSI RCATGY 1 cut(s) 765
BstPI GGTNACC 1 cut(s) 478
BstSFI CTRYAG 3 cut(s) 50, 873, 3023
BstSLI GKGCMC 2 cut(s) 2085, 2943
BstSNI TACGTA 2 cut(s) 1559, 1979
BstUI CGCG 1 cut(s) 2271
BstV2I GAAGAC 4 cut(s) 69, 2656, 2744, 2825
BstX2I RGATCY 8 cut(s) 70, 83, 827, 903, 1498, 1895, 2635, 3174
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 8 cut(s) 70, 83, 827, 903, 1498, 1895, 2635, 3174
BstZ17I GTATAC 1 cut(s) 3195
BsuI GTATCC 1 cut(s) 1817
BsuRI GGCC 6 cut(s) 167, 593, 656, 1703, 2011, 2388
BtgI CCRYGG 1 cut(s) 2857
BtsCI GGATG 2 cut(s) 460, 594
BtsI GCAGTG 3 cut(s) 214, 2569, 3032
BtsIMutI CAGTG 6 cut(s) 214, 420, 2363, 2569, 3032, 3250
BveI ACCTGC 2 cut(s) 43, 1954
Cac8I GCNNGC 9 cut(s) 33, 267, 1871, 1881, 1941, 2136, 2269, 2616, 2776
CaiI CAGNNNCTG 3 cut(s) 533, 563, 2534
CciI TCATGA 1 cut(s) 810
CfoI GCGC 3 cut(s) 472, 2103, 2477
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 9 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233, 2386
Cfr9I CCCGGG 2 cut(s) 126, 1724
CseI GACGC 4 cut(s) 144, 297, 1673, 2048
CsiI ACCWGGT 1 cut(s) 2725
Csp6I GTAC 7 cut(s) 1142, 1932, 2178, 2496, 2598, 2847, 3238
CviAII CATG 7 cut(s) 393, 762, 811, 1373, 1684, 2092, 3314
CviQI GTAC 7 cut(s) 1142, 1932, 2178, 2496, 2598, 2847, 3238
DriI GACNNNNNGTC 2 cut(s) 154, 376
EaeI YGGCCR 2 cut(s) 591, 1701
Eam1104I CTCTTC 2 cut(s) 550, 1089
Eam1105I GACNNNNNGTC 2 cut(s) 154, 376
EarI CTCTTC 2 cut(s) 550, 1089
EciI GGCGGA 1 cut(s) 2707
Ecl136II GAGCTC 1 cut(s) 1082
Eco105I TACGTA 2 cut(s) 1559, 1979
Eco130I CCWWGG 3 cut(s) 1181, 2229, 3369
Eco147I AGGCCT 1 cut(s) 2011
Eco24I GRGCYC 2 cut(s) 1084, 3159
Eco31I GGTCTC 1 cut(s) 3333
Eco32I GATATC 2 cut(s) 576, 1117
Eco47I GGWCC 8 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233
Eco47III AGCGCT 2 cut(s) 471, 2102
Eco53kI GAGCTC 1 cut(s) 1082
Eco57I CTGAAG 3 cut(s) 1092, 2772, 3348
Eco88I CYCGRG 4 cut(s) 95, 126, 1724, 3297
Eco91I GGTNACC 1 cut(s) 478
EcoICRI GAGCTC 1 cut(s) 1082
EcoO109I RGGNCCY 3 cut(s) 302, 1732, 2233
EcoO65I GGTNACC 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 487
EcoRII CCWGG 6 cut(s) 57, 79, 526, 1305, 2011, 2725
EcoRV GATATC 2 cut(s) 576, 1117
EcoT14I CCWWGG 3 cut(s) 1181, 2229, 3369
EcoT22I ATGCAT 1 cut(s) 1426
EcoT38I GRGCYC 2 cut(s) 1084, 3159
ErhI CCWWGG 3 cut(s) 1181, 2229, 3369
FaeI CATG 7 cut(s) 396, 765, 814, 1376, 1687, 2095, 3317
FalI AAGNNNNNCTT 2 cut(s) 1090, 1122
FaqI GGGAC 3 cut(s) 544, 1718, 2436
FatI CATG 7 cut(s) 392, 761, 810, 1372, 1683, 2091, 3313
FauI CCCGC 2 cut(s) 1743, 1862
FbaI TGATCA 2 cut(s) 442, 1074
FblI GTMKAC 2 cut(s) 1916, 3194
FokI GGATG 2 cut(s) 467, 601
FriOI GRGCYC 2 cut(s) 1084, 3159
GlaI GCGC 3 cut(s) 471, 2102, 2476
GsaI CCCAGC 3 cut(s) 2487, 2553, 3083
GsuI CTGGAG 5 cut(s) 494, 674, 1565, 2387, 2606
HaeII RGCGCY 3 cut(s) 473, 2104, 2478
HaeIII GGCC 6 cut(s) 167, 593, 656, 1703, 2011, 2388
HgaI GACGC 4 cut(s) 144, 297, 1673, 2048
HhaI GCGC 3 cut(s) 472, 2103, 2477
Hin1I GRCGYC 2 cut(s) 1399, 2059
Hin1II CATG 7 cut(s) 396, 765, 814, 1376, 1687, 2095, 3317
Hin6I GCGC 3 cut(s) 470, 2101, 2475
HinP1I GCGC 3 cut(s) 470, 2101, 2475
HincII GTYRAC 3 cut(s) 1211, 1277, 1917
HindII GTYRAC 3 cut(s) 1211, 1277, 1917
Hpy99I CGWCG 2 cut(s) 313, 1667
HpyCH4III ACNGT 5 cut(s) 118, 484, 600, 2361, 2858
HpyCH4IV ACGT 9 cut(s) 607, 1294, 1399, 1486, 1558, 1768, 1978, 2668, 3094
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 237, 713, 722, 964, 2963, 2972, 2981, 3163
HpySE526I ACGT 9 cut(s) 607, 1294, 1399, 1486, 1558, 1768, 1978, 2668, 3094
Hsp92I GRCGYC 2 cut(s) 1399, 2059
Hsp92II CATG 7 cut(s) 396, 765, 814, 1376, 1687, 2095, 3317
HspAI GCGC 3 cut(s) 470, 2101, 2475
KflI GGGWCCC 1 cut(s) 1732
Kpn2I TCCGGA 1 cut(s) 1970
KpnI GGTACC 2 cut(s) 1145, 2601
Ksp22I TGATCA 2 cut(s) 442, 1074
LguI GCTCTTC 1 cut(s) 1089
LweI GCATC 6 cut(s) 788, 1411, 1433, 2254, 2565, 2971
MabI ACCWGGT 1 cut(s) 2725
MaeII ACGT 9 cut(s) 607, 1294, 1399, 1486, 1558, 1768, 1978, 2668, 3094
MaeIII GTNAC 8 cut(s) 118, 146, 478, 614, 1368, 1407, 2162, 2361
MbiI CCGCTC 1 cut(s) 3221
MfeI CAATTG 3 cut(s) 2745, 2976, 3270
MflI RGATCY 8 cut(s) 70, 83, 827, 903, 1498, 1895, 2635, 3174
MhlI GDGCHC 7 cut(s) 1084, 1492, 1945, 2085, 2528, 2943, 3159
MlsI TGGCCA 2 cut(s) 593, 1703
MluI ACGCGT 1 cut(s) 2269
MluNI TGGCCA 2 cut(s) 593, 1703
MlyI GAGTC 7 cut(s) 386, 669, 1216, 1601, 1631, 1794, 2151
MmeI TCCRAC 4 cut(s) 894, 1795, 2524, 2626
Mox20I TGGCCA 2 cut(s) 593, 1703
Mph1103I ATGCAT 1 cut(s) 1426
MroI TCCGGA 1 cut(s) 1970
MroXI GAANNNNTTC 1 cut(s) 3387
MscI TGGCCA 2 cut(s) 593, 1703
MseI TTAA 9 cut(s) 248, 1226, 1986, 2238, 2259, 2879, 2948, 2996, 3108
Msp20I TGGCCA 2 cut(s) 593, 1703
MspA1I CMGCKG 1 cut(s) 560
MunI CAATTG 3 cut(s) 2745, 2976, 3270
MvaI CCWGG 6 cut(s) 59, 81, 528, 1307, 2013, 2727
MvnI CGCG 1 cut(s) 2271
MwoI GCNNNNNNNGC 9 cut(s) 28, 237, 713, 722, 964, 2963, 2972, 2981, 3163
NciI CCSGG 8 cut(s) 127, 128, 1725, 1726, 1811, 1857, 2056, 3161
NheI GCTAGC 1 cut(s) 2614
NlaIII CATG 7 cut(s) 396, 765, 814, 1376, 1687, 2095, 3317
NmeAIII GCCGAG 1 cut(s) 956
NmuCI GTSAC 5 cut(s) 118, 146, 478, 2162, 2361
NsiI ATGCAT 1 cut(s) 1426
NspI RCATGY 1 cut(s) 765
NspV TTCGAA 2 cut(s) 643, 3391
PaeR7I CTCGAG 2 cut(s) 95, 3297
PagI TCATGA 1 cut(s) 810
PaqCI CACCTGC 1 cut(s) 1954
PceI AGGCCT 1 cut(s) 2011
PciI ACATGT 1 cut(s) 761
PciSI GCTCTTC 1 cut(s) 1089
PcsI WCGNNNNNNNCGW 1 cut(s) 2344
PdmI GAANNNNTTC 1 cut(s) 3387
PfeI GAWTC 5 cut(s) 1043, 1175, 1259, 1509, 2250
PflFI GACNNNGTC 2 cut(s) 920, 2160
PflMI CCANNNNNTGG 1 cut(s) 2683
PfoI TCCNGGA 2 cut(s) 57, 3159
PleI GAGTC 7 cut(s) 385, 668, 1215, 1600, 1630, 1794, 2151
PpsI GAGTC 7 cut(s) 385, 668, 1215, 1600, 1630, 1794, 2151
Ppu21I YACGTR 3 cut(s) 1559, 1769, 1979
PpuMI RGGWCCY 3 cut(s) 302, 1732, 2233
PscI ACATGT 1 cut(s) 761
PsiI TTATAA 1 cut(s) 569
Psp124BI GAGCTC 1 cut(s) 1084
Psp5II RGGWCCY 3 cut(s) 302, 1732, 2233
Psp6I CCWGG 6 cut(s) 57, 79, 526, 1305, 2011, 2725
PspEI GGTNACC 1 cut(s) 478
PspFI CCCAGC 3 cut(s) 2483, 2549, 3079
PspGI CCWGG 6 cut(s) 57, 79, 526, 1305, 2011, 2725
PspPI GGNCC 9 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233, 2386
PspPPI RGGWCCY 3 cut(s) 302, 1732, 2233
PspXI VCTCGAGB 1 cut(s) 95
PstI CTGCAG 3 cut(s) 54, 877, 3027
PstNI CAGNNNCTG 3 cut(s) 533, 563, 2534
PsuI RGATCY 8 cut(s) 70, 83, 827, 903, 1498, 1895, 2635, 3174
PsyI GACNNNGTC 2 cut(s) 920, 2160
RsaI GTAC 7 cut(s) 1143, 1933, 2179, 2497, 2599, 2848, 3239
RsaNI GTAC 7 cut(s) 1142, 1932, 2178, 2496, 2598, 2847, 3238
SacI GAGCTC 1 cut(s) 1084
SalI GTCGAC 1 cut(s) 1915
SapI GCTCTTC 1 cut(s) 1089
SaqAI TTAA 9 cut(s) 248, 1226, 1986, 2238, 2259, 2879, 2948, 2996, 3108
Sau96I GGNCC 9 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233, 2386
SchI GAGTC 7 cut(s) 386, 669, 1216, 1601, 1631, 1794, 2151
SduI GDGCHC 7 cut(s) 1084, 1492, 1945, 2085, 2528, 2943, 3159
SexAI ACCWGGT 1 cut(s) 2725
SfaNI GCATC 6 cut(s) 788, 1411, 1433, 2254, 2565, 2971
SfcI CTRYAG 3 cut(s) 50, 873, 3023
Sfr274I CTCGAG 2 cut(s) 95, 3297
SfuI TTCGAA 2 cut(s) 643, 3391
SinI GGWCC 8 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233
SlaI CTCGAG 2 cut(s) 95, 3297
SmaI CCCGGG 2 cut(s) 128, 1726
SmlI CTYRAG 4 cut(s) 95, 847, 2462, 3297
SmoI CTYRAG 4 cut(s) 95, 847, 2462, 3297
SnaBI TACGTA 2 cut(s) 1559, 1979
SseBI AGGCCT 1 cut(s) 2011
SsiI CCGC 7 cut(s) 241, 560, 1736, 1869, 2692, 2778, 3219
SspI AATATT 2 cut(s) 1573, 1852
SstI GAGCTC 1 cut(s) 1084
StuI AGGCCT 1 cut(s) 2011
StyI CCWWGG 3 cut(s) 1181, 2229, 3369
TaaI ACNGT 5 cut(s) 118, 484, 600, 2361, 2858
TaiI ACGT 9 cut(s) 610, 1297, 1402, 1489, 1561, 1771, 1981, 2671, 3097
TaqI TCGA 9 cut(s) 96, 380, 584, 643, 1916, 2377, 2868, 3298, 3391
TaqII GACCGA 1 cut(s) 2150
TatI WGTACW 1 cut(s) 3237
TauI GCSGC 3 cut(s) 243, 563, 3221
TfiI GAWTC 5 cut(s) 1043, 1175, 1259, 1509, 2250
Tru1I TTAA 9 cut(s) 248, 1226, 1986, 2238, 2259, 2879, 2948, 2996, 3108
Tru9I TTAA 9 cut(s) 248, 1226, 1986, 2238, 2259, 2879, 2948, 2996, 3108
TscAI CASTG 6 cut(s) 214, 427, 2370, 2569, 3032, 3250
TseFI GTSAC 5 cut(s) 118, 146, 478, 2162, 2361
Tsp45I GTSAC 5 cut(s) 118, 146, 478, 2162, 2361
TspGWI ACGGA 4 cut(s) 188, 840, 1395, 1460
TspMI CCCGGG 2 cut(s) 126, 1724
TspRI CASTG 6 cut(s) 214, 427, 2370, 2569, 3032, 3250
Tth111I GACNNNGTC 2 cut(s) 920, 2160
Van91I CCANNNNNTGG 1 cut(s) 2683
VneI GTGCAC 1 cut(s) 2939
VpaK11BI GGWCC 8 cut(s) 55, 76, 161, 196, 302, 916, 1732, 2233
XapI RAATTY 6 cut(s) 487, 620, 1311, 1549, 2467, 2679
XbaI TCTAGA 2 cut(s) 1993, 3046
XceI RCATGY 1 cut(s) 765
XcmI CCANNNNNNNNNTGG 1 cut(s) 2396
XhoI CTCGAG 2 cut(s) 95, 3297
XmaI CCCGGG 2 cut(s) 126, 1724
XmiI GTMKAC 2 cut(s) 1916, 3194
XmnI GAANNNNTTC 1 cut(s) 3387
ZraI GACGTC 1 cut(s) 1400
Zsp2I ATGCAT 1 cut(s) 1426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.