Rmu_ssc0000215.1_g000028

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000215.1
OG0004519
Physical Location & Seq
Reverse (-)
114119 .. 115447
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000215.1_g000028.1.cds

Sequence Viewer

Length: 1329 bp
atggcgagaacatgctcccaggatgtgttgtccgacattctttgtagattgcacgtaaaatctttgaagagattccaatgtgtctgcaaatcttggtatgctctaattaggcacccaagttttgtcaccaaacacctccactttcacagcagacagaacaagaatactcgttccttcctgcattctcactccctggatcaacgttacagttatgagttgcccattgatcaacgaaaagacacttgtttcagcttggtcaatttagacgaaaccactggtggtattgtttctcttgattggcttaagttttcagatatttcagaccaaggaaacaagtttccgaattattggtatggagatgtagaaatttctggtccacgcaatggcatatattgcctgcatgaagcatctcaagatcacagcgatgtaattgcactggtgaatatttcgttgagagaattcaagctagttccaaaaccagaacaatcgattatttggcatccaggttattttgcactagactgggtcgaaattggttttgatcctaaaaccaatgattacaaggtgttttttttcaaggaattatgcagtactgatacccaggaaagtgaacgacaggctgcaatatatcatttaagttctaattcatggaaaaagctaagtgctcctttgcttgagcatgttattctatattttcgagattgtaatattactgacacctatgtcaacggacgtgttcactggttgggttttgaggactaccctggtcgcagagattggtgtattctttcttttgacatggttagtgaagtgtttgcaaagataaagcttccagacttgagtattaattgggacgaaactgaatatcttcttgcagtgtttaatgaatctctttcccttattctttatccaaagacgccagattatcccatgtttacaggttacattaaatgttttgacatatgggttatgaatgactatggtaacgacagatcttggactaagcttttcacaactggaccagttgcatctgcttacagactactaggtttttggcgaaatggcgactttcttatacaacatgattttggatacaccatctcttacgacccaggtacccaagagatcgagccgctttgttttggtgaagggcttcatttgaaccaatctcgtatctactcagagagcctagtttcagtgacggggaatgcacgaaatgattttgaaatagtagttgaagactttcccttcgtcatgactccagacatgaagagagtcctagactacatagagaaatgtccatcttgttcccgtttaacttctgattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

442

Amino Acids

51.51

Weight (kDa)

5.53

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 722
Acc65I GGTACC 1 cut(s) 1115
AccB1I GGYRCC 2 cut(s) 111, 1115
AccB7I CCANNNNNTGG 1 cut(s) 383
AciI CCGC 1 cut(s) 1133
AclI AACGTT 1 cut(s) 202
AclWI GGATC 2 cut(s) 204, 536
AcsI RAATTY 2 cut(s) 366, 458
AcyI GRCGYC 1 cut(s) 917
AfaI GTAC 2 cut(s) 592, 1117
AfiI CCNNNNNNNGG 1 cut(s) 383
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 733
AgsI TTSAA 6 cut(s) 67, 463, 577, 1162, 1226, 1238
AjiI CACGTC 1 cut(s) 734
AjnI CCWGG 6 cut(s) 18, 192, 502, 600, 763, 1111
AluBI AGCT 5 cut(s) 252, 466, 658, 829, 1006
AluI AGCT 5 cut(s) 252, 466, 658, 829, 1006
Alw21I GWGCWC 1 cut(s) 667
AlwI GGATC 2 cut(s) 204, 536
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 2 cut(s) 366, 458
ArsI GACNNNNNNTTYG 2 cut(s) 1232, 1264
AseI ATTAAT 1 cut(s) 846
Asp700I GAANNNNTTC 4 cut(s) 71, 867, 1152, 1242
Asp718I GGTACC 1 cut(s) 1115
AspS9I GGNCC 2 cut(s) 374, 1019
AsuHPI GGTGA 3 cut(s) 118, 451, 1157
AvaII GGWCC 2 cut(s) 374, 1019
BaeI ACNNNNGTAYC 2 cut(s) 1099, 1132
BanI GGYRCC 2 cut(s) 111, 1115
BbsI GAAGAC 1 cut(s) 1245
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 1 cut(s) 607
BccI CCATC 2 cut(s) 1106, 1309
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 6 cut(s) 20, 194, 504, 602, 765, 1113
BciVI GTATCC 1 cut(s) 1085
BclI TGATCA 1 cut(s) 226
BfaI CTAG 5 cut(s) 467, 518, 1046, 1190, 1280
BfrI CTTAAG 1 cut(s) 302
BfuI GTATCC 1 cut(s) 1085
BglII AGATCT 1 cut(s) 992
BisI GCNGC 2 cut(s) 621, 1133
BlsI GCNGC 2 cut(s) 622, 1134
BmcAI AGTACT 1 cut(s) 592
Bme1390I CCNGG 6 cut(s) 20, 194, 504, 602, 765, 1113
Bme18I GGWCC 2 cut(s) 374, 1019
BmgBI CACGTC 1 cut(s) 734
BmgT120I GGNCC 2 cut(s) 374, 1019
BmiI GGNNCC 2 cut(s) 113, 1117
BmrFI CCNGG 6 cut(s) 20, 194, 504, 602, 765, 1113
BmrI ACTGGG 1 cut(s) 532
BmsI GCATC 3 cut(s) 416, 508, 1037
BmuI ACTGGG 1 cut(s) 532
BpiI GAAGAC 1 cut(s) 1245
BpmI CTGGAG 1 cut(s) 1245
BpuEI CTTGAG 3 cut(s) 396, 695, 859
Bsa29I ATCGAT 1 cut(s) 488
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 917
BsaJI CCNNGG 6 cut(s) 18, 192, 325, 600, 763, 1111
Bsc4I CCNNNNNNNGG 1 cut(s) 383
Bse1I ACTGG 6 cut(s) 280, 441, 527, 746, 1021, 1022
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 6 cut(s) 20, 194, 504, 602, 765, 1113
BseCI ATCGAT 1 cut(s) 488
BseDI CCNNGG 6 cut(s) 18, 192, 325, 600, 763, 1111
BseGI GGATG 2 cut(s) 28, 499
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 1194
BseNI ACTGG 6 cut(s) 280, 441, 527, 746, 1021, 1022
BseXI GCAGC 1 cut(s) 607
BshNI GGYRCC 2 cut(s) 111, 1115
BshVI ATCGAT 1 cut(s) 488
BsiHKAI GWGCWC 1 cut(s) 667
BslFI GGGAC 1 cut(s) 866
BslI CCNNNNNNNGG 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 866
BsmI GAATGC 2 cut(s) 181, 1213
Bsp1286I GDGCHC 1 cut(s) 667
Bsp143I GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
BspACI CCGC 1 cut(s) 1133
BspCNI CTCAG 1 cut(s) 1193
BspDI ATCGAT 1 cut(s) 488
BspHI TCATGA 1 cut(s) 1254
BspLI GGNNCC 2 cut(s) 113, 1117
BspPI GGATC 2 cut(s) 204, 536
BspT107I GGYRCC 2 cut(s) 111, 1115
BspTI CTTAAG 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 388
BsrI ACTGG 6 cut(s) 280, 441, 527, 746, 1021, 1022
BssECI CCNNGG 6 cut(s) 18, 192, 325, 600, 763, 1111
BssMI GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
BssNI GRCGYC 1 cut(s) 917
BssT1I CCWWGG 1 cut(s) 325
Bst2UI CCWGG 6 cut(s) 20, 194, 504, 602, 765, 1113
Bst4CI ACNGT 1 cut(s) 209
Bst6I CTCTTC 2 cut(s) 62, 1265
BstACI GRCGYC 1 cut(s) 917
BstAFI CTTAAG 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 3 cut(s) 659, 1002, 1180
BstF5I GGATG 2 cut(s) 28, 499
BstKTI GATC 6 cut(s) 199, 229, 418, 544, 995, 1128
BstMBI GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNI CCWGG 6 cut(s) 20, 194, 504, 602, 765, 1113
BstNSI RCATGY 2 cut(s) 15, 683
BstSCI CCNGG 6 cut(s) 18, 192, 502, 600, 763, 1111
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 1 cut(s) 1245
BstX2I RGATCY 1 cut(s) 992
BstYI RGATCY 1 cut(s) 992
Bsu15I ATCGAT 1 cut(s) 488
BsuI GTATCC 1 cut(s) 1085
BsuTUI ATCGAT 1 cut(s) 488
BtgZI GCGATG 1 cut(s) 438
BtrI CACGTC 1 cut(s) 734
BtsCI GGATG 2 cut(s) 28, 499
BtsI GCAGTG 1 cut(s) 882
BtsIMutI CAGTG 5 cut(s) 273, 434, 739, 882, 1203
Cac8I GCNNGC 1 cut(s) 398
CciI TCATGA 1 cut(s) 1254
Cfr13I GGNCC 2 cut(s) 374, 1019
ClaI ATCGAT 1 cut(s) 488
CseI GACGC 1 cut(s) 925
Csp6I GTAC 2 cut(s) 591, 1116
CviAII CATG 9 cut(s) 12, 401, 648, 680, 799, 931, 1082, 1255, 1267
CviQI GTAC 2 cut(s) 591, 1116
DdeI CTNAG 3 cut(s) 659, 1002, 1180
DpnI GATC 6 cut(s) 198, 228, 417, 543, 994, 1127
DpnII GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
DrdI GACNNNNNNGTC 1 cut(s) 722
DseDI GACNNNNNNGTC 1 cut(s) 722
Eam1104I CTCTTC 2 cut(s) 62, 1265
EarI CTCTTC 2 cut(s) 62, 1265
Eco130I CCWWGG 1 cut(s) 325
Eco47I GGWCC 2 cut(s) 374, 1019
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 6 cut(s) 18, 192, 502, 600, 763, 1111
EcoT14I CCWWGG 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 9 cut(s) 15, 404, 651, 683, 802, 934, 1085, 1258, 1270
FalI AAGNNNNNCTT 2 cut(s) 652, 684
FaqI GGGAC 1 cut(s) 866
FatI CATG 9 cut(s) 11, 400, 647, 679, 798, 930, 1081, 1254, 1266
FauNDI CATATG 1 cut(s) 962
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 2 cut(s) 621, 1133
FokI GGATG 2 cut(s) 35, 486
Fsp4HI GCNGC 2 cut(s) 621, 1133
FspBI CTAG 5 cut(s) 467, 518, 1046, 1190, 1280
GluI GCNGC 2 cut(s) 621, 1133
GsuI CTGGAG 1 cut(s) 1245
HgaI GACGC 1 cut(s) 925
Hin1I GRCGYC 1 cut(s) 917
Hin1II CATG 9 cut(s) 15, 404, 651, 683, 802, 934, 1085, 1258, 1270
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 2 cut(s) 827, 1004
HinfI GANTC 4 cut(s) 72, 887, 1258, 1275
HphI GGTGA 3 cut(s) 118, 451, 1157
Hpy166II GTNNAC 5 cut(s) 377, 611, 727, 739, 936
Hpy188I TCNGA 6 cut(s) 34, 313, 322, 342, 1183, 1324
Hpy188III TCNNGA 6 cut(s) 293, 413, 698, 833, 1255, 1262
Hpy8I GTNNAC 5 cut(s) 377, 611, 727, 739, 936
HpyAV CCTTC 3 cut(s) 184, 1142, 1258
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4IV ACGT 3 cut(s) 54, 202, 733
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 3 cut(s) 659, 1002, 1180
HpySE526I ACGT 3 cut(s) 54, 202, 733
Hsp92I GRCGYC 1 cut(s) 917
Hsp92II CATG 9 cut(s) 15, 404, 651, 683, 802, 934, 1085, 1258, 1270
KpnI GGTACC 1 cut(s) 1119
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
LmnI GCTCC 2 cut(s) 20, 670
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 3 cut(s) 416, 508, 1037
MaeI CTAG 5 cut(s) 467, 518, 1046, 1190, 1280
MaeII ACGT 3 cut(s) 54, 202, 733
MaeIII GTNAC 5 cut(s) 124, 203, 941, 983, 1198
MalI GATC 6 cut(s) 198, 228, 417, 543, 994, 1127
MboI GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
MboII GAAGA 4 cut(s) 79, 860, 1250, 1282
MflI RGATCY 1 cut(s) 992
MhlI GDGCHC 1 cut(s) 667
MlyI GAGTC 2 cut(s) 1252, 1284
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 2 cut(s) 146, 748
MroXI GAANNNNTTC 4 cut(s) 71, 867, 1152, 1242
MseI TTAA 6 cut(s) 303, 635, 846, 882, 948, 1316
MslI CAYNNNNRTG 1 cut(s) 423
MspCI CTTAAG 1 cut(s) 302
MspR9I CCNGG 6 cut(s) 20, 194, 504, 602, 765, 1113
Mva1269I GAATGC 2 cut(s) 181, 1213
MvaI CCWGG 6 cut(s) 20, 194, 504, 602, 765, 1113
MwoI GCNNNNNNNGC 1 cut(s) 393
NdeI CATATG 1 cut(s) 962
NdeII GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
NlaIII CATG 9 cut(s) 15, 404, 651, 683, 802, 934, 1085, 1258, 1270
NlaIV GGNNCC 2 cut(s) 113, 1117
NmuCI GTSAC 2 cut(s) 124, 1198
NspI RCATGY 2 cut(s) 15, 683
PagI TCATGA 1 cut(s) 1254
PctI GAATGC 2 cut(s) 181, 1213
PdmI GAANNNNTTC 4 cut(s) 71, 867, 1152, 1242
PfeI GAWTC 2 cut(s) 72, 887
PflFI GACNNNGTC 1 cut(s) 524
PflMI CCANNNNNTGG 1 cut(s) 383
PkrI GCNGC 2 cut(s) 622, 1134
PleI GAGTC 2 cut(s) 1252, 1283
PpsI GAGTC 2 cut(s) 1252, 1283
Ppu21I YACGTR 1 cut(s) 55
PshBI ATTAAT 1 cut(s) 846
Psp1406I AACGTT 1 cut(s) 202
Psp6I CCWGG 6 cut(s) 18, 192, 502, 600, 763, 1111
PspGI CCWGG 6 cut(s) 18, 192, 502, 600, 763, 1111
PspN4I GGNNCC 2 cut(s) 113, 1117
PspPI GGNCC 2 cut(s) 374, 1019
PsuI RGATCY 1 cut(s) 992
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 2 cut(s) 592, 1117
RsaNI GTAC 2 cut(s) 591, 1116
RseI CAYNNNNRTG 1 cut(s) 423
SaqAI TTAA 6 cut(s) 303, 635, 846, 882, 948, 1316
SatI GCNGC 2 cut(s) 621, 1133
Sau3AI GATC 6 cut(s) 196, 226, 415, 541, 992, 1125
Sau96I GGNCC 2 cut(s) 374, 1019
ScaI AGTACT 1 cut(s) 592
SchI GAGTC 2 cut(s) 1252, 1284
ScrFI CCNGG 6 cut(s) 20, 194, 504, 602, 765, 1113
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 3 cut(s) 416, 508, 1037
SinI GGWCC 2 cut(s) 374, 1019
SmiMI CAYNNNNRTG 1 cut(s) 423
SmlI CTYRAG 4 cut(s) 302, 411, 674, 838
SmoI CTYRAG 4 cut(s) 302, 411, 674, 838
SsiI CCGC 1 cut(s) 1133
SspI AATATT 2 cut(s) 445, 709
SspMI CTAG 5 cut(s) 467, 518, 1046, 1190, 1280
StyD4I CCNGG 6 cut(s) 18, 192, 502, 600, 763, 1111
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 1 cut(s) 209
TaiI ACGT 3 cut(s) 57, 205, 736
TaqI TCGA 4 cut(s) 488, 528, 697, 1128
TatI WGTACW 1 cut(s) 590
TauI GCSGC 1 cut(s) 1135
TfiI GAWTC 2 cut(s) 72, 887
Tru1I TTAA 6 cut(s) 303, 635, 846, 882, 948, 1316
Tru9I TTAA 6 cut(s) 303, 635, 846, 882, 948, 1316
TscAI CASTG 5 cut(s) 280, 441, 746, 882, 1203
TseFI GTSAC 2 cut(s) 124, 1198
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 2 cut(s) 124, 1198
TspDTI ATGAA 6 cut(s) 417, 636, 900, 986, 1145, 1283
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 5 cut(s) 280, 441, 746, 882, 1203
Tth111I GACNNNGTC 1 cut(s) 524
Van91I CCANNNNNTGG 1 cut(s) 383
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 2 cut(s) 374, 1019
VspI ATTAAT 1 cut(s) 846
XapI RAATTY 2 cut(s) 366, 458
XceI RCATGY 2 cut(s) 15, 683
XmnI GAANNNNTTC 4 cut(s) 71, 867, 1152, 1242
XspI CTAG 5 cut(s) 467, 518, 1046, 1190, 1280
ZrmI AGTACT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.