Rorug02G0061200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004519
Physical Location & Seq
Reverse (-)
4729415 .. 4730395
981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0061200.1

Sequence Viewer

Length: 582 bp
ATGCATATGCCCGGCCTCTTAGAAGAGATATCCTTGAACCAAGCTTCACTTGTTAGCTCTCAAATGGAACCTACTACCCAAATGGCTCATGACCAGCAACAACCAAAATGGGAAGGCGAGGCTAGGGCAGAGCTAATAGGCCCTAAGGCAGAACAAGTGTGGCCTTTGTTGGAGGACTTCTTCGGCCTACACAAGTGGTTCCCAACCCTAACCACTTGCCTTCCTGTACAAGGCGTATCCGGTCAACCAGGCTGCGTGCGCTATTGCGCCGGATTCAAAACTCCGGTTGATCATGAAAGCAACCAGCCGGAGCATCTGAACTGGACTAAGCAGGAGCTACTGTCCATAGACCCAAGCCAGCTGACGTTTAGCTATTCCATTGTAGACGGGAATGTCGGGTTCAACTCGTACATTTCGACTGTGCGAGTGGTGCCGAAGGAAGGTGGATGCAGCATTGAGTGGAAGTATGAGGTTGAGCCAGTGGAGGGGTGGACACTGAAGGATCTGGACGTGTTTATAGGCACTGGTTTGCAAGTCATGGCTAGCAGAATGGAAGCTAGCCTCTCCAAACTCCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

193

Amino Acids

21.57

Weight (kDa)

4.8

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 49 - 168 1.1e-19 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 430
AccI GTMKAC 1 cut(s) 384
AclWI GGATC 1 cut(s) 510
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 2 cut(s) 228, 410
AfiI CCNNNNNNNGG 3 cut(s) 230, 440, 485
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 3 cut(s) 37, 277, 403
AjiI CACGTC 1 cut(s) 511
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 7 cut(s) 44, 57, 133, 337, 361, 372, 557
AluI AGCT 7 cut(s) 44, 57, 133, 337, 361, 372, 557
AlwI GGATC 1 cut(s) 510
AoxI GGCC 4 cut(s) 13, 139, 161, 184
ApeKI GCWGC 2 cut(s) 252, 450
AspLEI GCGC 2 cut(s) 261, 269
AspS9I GGNCC 1 cut(s) 140
AsuC2I CCSGG 1 cut(s) 12
AsuNHI GCTAGC 2 cut(s) 542, 557
AxyI CCTNAGG 1 cut(s) 144
BanI GGYRCC 1 cut(s) 430
BbvI GCAGC 2 cut(s) 239, 462
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 1 cut(s) 247
BclI TGATCA 1 cut(s) 289
BcnI CCSGG 1 cut(s) 12
BfaI CTAG 3 cut(s) 123, 543, 558
BfuI GTATCC 1 cut(s) 247
BisI GCNGC 2 cut(s) 253, 451
BlsI GCNGC 2 cut(s) 254, 452
Bme1390I CCNGG 2 cut(s) 12, 249
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 69, 200, 432
BmrFI CCNGG 2 cut(s) 12, 249
BmsI GCATC 2 cut(s) 322, 437
BmtI GCTAGC 2 cut(s) 546, 561
BpuMI CCSGG 1 cut(s) 12
BsaWI WCCGGW 2 cut(s) 239, 283
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 440, 485
Bse1I ACTGG 3 cut(s) 326, 479, 529
Bse21I CCTNAGG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 452
BseLI CCNNNNNNNGG 3 cut(s) 230, 440, 485
BseNI ACTGG 3 cut(s) 326, 479, 529
BseXI GCAGC 2 cut(s) 239, 462
BshFI GGCC 4 cut(s) 15, 141, 163, 186
BshNI GGYRCC 1 cut(s) 430
BsiSI CCGG 5 cut(s) 12, 240, 270, 284, 308
BslI CCNNNNNNNGG 3 cut(s) 230, 440, 485
BsnI GGCC 4 cut(s) 15, 141, 163, 186
Bsp1407I TGTACA 1 cut(s) 226
Bsp143I GATC 2 cut(s) 289, 502
BspANI GGCC 4 cut(s) 15, 141, 163, 186
BspHI TCATGA 2 cut(s) 88, 292
BspLI GGNNCC 3 cut(s) 69, 200, 432
BspOI GCTAGC 2 cut(s) 546, 561
BspPI GGATC 1 cut(s) 510
BspT107I GGYRCC 1 cut(s) 430
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 3 cut(s) 326, 479, 529
BssMI GATC 2 cut(s) 289, 502
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 342, 421
Bst6I CTCTTC 1 cut(s) 18
BstAUI TGTACA 1 cut(s) 226
BstC8I GCNNGC 4 cut(s) 257, 359, 544, 559
BstDEI CTNAG 3 cut(s) 19, 144, 327
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 1 cut(s) 452
BstHHI GCGC 2 cut(s) 261, 269
BstKTI GATC 2 cut(s) 292, 505
BstMBI GATC 2 cut(s) 289, 502
BstMWI GCNNNNNNNGC 2 cut(s) 258, 430
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 2 cut(s) 10, 247
BstV1I GCAGC 2 cut(s) 239, 462
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
Bsu36I CCTNAGG 1 cut(s) 144
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 4 cut(s) 15, 141, 163, 186
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 1 cut(s) 452
BtsIMutI CAGTG 3 cut(s) 486, 494, 522
Cac8I GCNNGC 4 cut(s) 257, 359, 544, 559
CciI TCATGA 2 cut(s) 88, 292
CfoI GCGC 2 cut(s) 261, 269
Cfr13I GGNCC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 227, 409
CviAII CATG 3 cut(s) 89, 293, 538
CviQI GTAC 2 cut(s) 227, 409
DdeI CTNAG 3 cut(s) 19, 144, 327
DpnI GATC 2 cut(s) 291, 504
DpnII GATC 2 cut(s) 289, 502
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco32I GATATC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 518
Eco81I CCTNAGG 1 cut(s) 144
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 247
EcoRV GATATC 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 92, 296, 541
FaiI YATR 8 cut(s) 6, 8, 90, 294, 347, 468, 518, 539
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FatI CATG 3 cut(s) 88, 292, 537
FauNDI CATATG 1 cut(s) 6
FbaI TGATCA 1 cut(s) 289
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 2 cut(s) 253, 451
FokI GGATG 1 cut(s) 459
Fsp4HI GCNGC 2 cut(s) 253, 451
FspBI CTAG 3 cut(s) 123, 543, 558
GlaI GCGC 2 cut(s) 260, 268
GluI GCNGC 2 cut(s) 253, 451
HaeIII GGCC 4 cut(s) 15, 141, 163, 186
HapII CCGG 5 cut(s) 12, 240, 270, 284, 308
HhaI GCGC 2 cut(s) 261, 269
Hin1II CATG 3 cut(s) 92, 296, 541
Hin6I GCGC 2 cut(s) 259, 267
HinP1I GCGC 2 cut(s) 259, 267
HincII GTYRAC 1 cut(s) 245
HindII GTYRAC 1 cut(s) 245
HindIII AAGCTT 1 cut(s) 42
HinfI GANTC 1 cut(s) 273
HpaII CCGG 5 cut(s) 12, 240, 270, 284, 308
Hpy166II GTNNAC 3 cut(s) 245, 385, 492
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 3 cut(s) 89, 293, 506
Hpy8I GTNNAC 3 cut(s) 245, 385, 492
HpyAV CCTTC 5 cut(s) 107, 230, 430, 434, 493
HpyCH4III ACNGT 2 cut(s) 342, 421
HpyCH4IV ACGT 2 cut(s) 365, 510
HpyCH4V TGCA 3 cut(s) 4, 450, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 430
HpyF3I CTNAG 3 cut(s) 19, 144, 327
HpySE526I ACGT 2 cut(s) 365, 510
Hsp92II CATG 3 cut(s) 92, 296, 541
HspAI GCGC 2 cut(s) 259, 267
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 2 cut(s) 289, 502
LmnI GCTCC 2 cut(s) 310, 334
Lsp1109I GCAGC 2 cut(s) 239, 462
LweI GCATC 2 cut(s) 322, 437
MaeI CTAG 3 cut(s) 123, 543, 558
MaeII ACGT 2 cut(s) 365, 510
MalI GATC 2 cut(s) 291, 504
MboI GATC 2 cut(s) 289, 502
MboII GAAGA 2 cut(s) 35, 172
MflI RGATCY 1 cut(s) 502
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 6 cut(s) 26, 112, 166, 463, 478, 572
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 361
MspI CCGG 5 cut(s) 12, 240, 270, 284, 308
MspR9I CCNGG 2 cut(s) 12, 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 258, 430
NciI CCSGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 6
NdeII GATC 2 cut(s) 289, 502
NheI GCTAGC 2 cut(s) 542, 557
NlaIII CATG 3 cut(s) 92, 296, 541
NlaIV GGNNCC 3 cut(s) 69, 200, 432
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 2 cut(s) 88, 292
PcsI WCGNNNNNNNCGW 1 cut(s) 413
PfeI GAWTC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 254, 452
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 3 cut(s) 69, 200, 432
PspPI GGNCC 1 cut(s) 140
PsuI RGATCY 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 361
RsaI GTAC 2 cut(s) 228, 410
RsaNI GTAC 2 cut(s) 227, 409
SatI GCNGC 2 cut(s) 253, 451
Sau3AI GATC 2 cut(s) 289, 502
Sau96I GGNCC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 12, 249
SfaNI GCATC 2 cut(s) 322, 437
SspMI CTAG 3 cut(s) 123, 543, 558
StyD4I CCNGG 2 cut(s) 10, 247
TaaI ACNGT 2 cut(s) 342, 421
TaiI ACGT 2 cut(s) 368, 513
TaqI TCGA 1 cut(s) 416
TatI WGTACW 1 cut(s) 226
TfiI GAWTC 1 cut(s) 273
TscAI CASTG 3 cut(s) 486, 501, 529
TseI GCWGC 2 cut(s) 252, 450
TspDTI ATGAA 1 cut(s) 309
TspRI CASTG 3 cut(s) 486, 501, 529
XagI CCTNNNNNAGG 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 486
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 3 cut(s) 123, 543, 558
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.