Rh5BG064900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004519
Physical Location & Seq
Forward (+)
5362617 .. 5364248
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG064900.1

Sequence Viewer

Length: 540 bp
ATGGAACTTTCTCAAATCTCAAACAACTTTTTTCACCTTCTCACATATACACACACAAATCATAGACACAAAATGGTGACTCACATAGCCATTGAACTGTCGAGCAGCTGCTTCTACTTCCTAAGCACTCGACATGGTCATAAACCTGAATCCCCTGCTTCTCCCAGTCGTCTTGTCATATATCACCTGCAAAATCCATCAAAAAGACTCCATTTATCCCAAAAAAAAACATTGAACCCACAATTCATCTCCATTCAATTCAGAAAAAGATGCAAAATTTCAAATGAAAAACAAAACCATACCTCATCCATCTCAACATTTCCAGCACCTTCGAAGAGCTCAGTTAGCGACAGTGAGGGGAAGGTTTCCGATGAAGAGCTTGAGGTTAGGCGAGAAATTGGGCTCGCCGGCGTCGTCGCTGAGAACCTCATCATCCTCCTGGTAACCTCCGCCGCTCTTCTACCTCCACTTTCGAATTCATCGTTATCCGAATCTGAATCGGACTCCAACCCAACAACCCGAAAACCCTTGTGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.09

Weight (kDa)

9.36

Isoelectric Point (pI)

67.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 195
AccBSI CCGCTC 1 cut(s) 455
AciI CCGC 2 cut(s) 450, 453
AcsI RAATTY 2 cut(s) 276, 475
AcyI GRCGYC 1 cut(s) 411
AgsI TTSAA 4 cut(s) 95, 235, 257, 282
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 4 cut(s) 108, 339, 379, 537
AluI AGCT 4 cut(s) 108, 339, 379, 537
Alw21I GWGCWC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 105, 108
ApoI RAATTY 2 cut(s) 276, 475
AsuHPI GGTGA 3 cut(s) 26, 88, 176
AsuII TTCGAA 2 cut(s) 332, 473
BanII GRGCYC 2 cut(s) 341, 405
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 2 cut(s) 95, 117
BccI CCATC 2 cut(s) 205, 317
BciT130I CCWGG 1 cut(s) 440
BfaI CTAG 1 cut(s) 538
BfuAI ACCTGC 1 cut(s) 195
BisI GCNGC 3 cut(s) 106, 109, 453
BlsI GCNGC 3 cut(s) 107, 110, 454
Bme1390I CCNGG 1 cut(s) 440
BmrFI CCNGG 1 cut(s) 440
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 260
BmuI ACTGGG 1 cut(s) 159
Bpu10I CCTNAGC 1 cut(s) 122
Bpu14I TTCGAA 2 cut(s) 332, 473
BpuEI CTTGAG 1 cut(s) 401
BsaHI GRCGYC 1 cut(s) 411
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 165
BseBI CCWGG 1 cut(s) 440
BseGI GGATG 2 cut(s) 305, 432
BseMII CTCAG 2 cut(s) 354, 411
BseNI ACTGG 1 cut(s) 165
BseXI GCAGC 2 cut(s) 95, 117
BsiHKAI GWGCWC 1 cut(s) 341
BsiSI CCGG 1 cut(s) 408
Bsp119I TTCGAA 2 cut(s) 332, 473
Bsp1286I GDGCHC 2 cut(s) 341, 405
BspACI CCGC 2 cut(s) 450, 453
BspCNI CTCAG 2 cut(s) 353, 412
BspMI ACCTGC 1 cut(s) 195
BspQI GCTCTTC 3 cut(s) 329, 369, 462
BspT104I TTCGAA 2 cut(s) 332, 473
BsrBI CCGCTC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 1 cut(s) 165
BssAI RCCGGY 1 cut(s) 407
BssNI GRCGYC 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 2 cut(s) 99, 353
Bst6I CTCTTC 3 cut(s) 329, 369, 462
BstACI GRCGYC 1 cut(s) 411
BstBI TTCGAA 2 cut(s) 332, 473
BstC8I GCNNGC 2 cut(s) 405, 409
BstDEI CTNAG 3 cut(s) 122, 340, 420
BstEII GGTNACC 1 cut(s) 442
BstF5I GGATG 2 cut(s) 305, 432
BstMWI GCNNNNNNNGC 1 cut(s) 345
BstNI CCWGG 1 cut(s) 440
BstPI GGTNACC 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 438
BstV1I GCAGC 2 cut(s) 95, 117
BtsCI GGATG 2 cut(s) 305, 432
BtsIMutI CAGTG 1 cut(s) 358
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 405, 409
Cfr10I RCCGGY 1 cut(s) 407
CseI GACGC 1 cut(s) 400
CviAII CATG 1 cut(s) 134
CviJI RGCY 6 cut(s) 89, 108, 339, 379, 403, 537
CviKI_1 RGCY 6 cut(s) 89, 108, 339, 379, 403, 537
DdeI CTNAG 3 cut(s) 122, 340, 420
Eam1104I CTCTTC 3 cut(s) 329, 369, 462
EarI CTCTTC 3 cut(s) 329, 369, 462
EciI GGCGGA 1 cut(s) 439
Ecl136II GAGCTC 1 cut(s) 339
Eco24I GRGCYC 2 cut(s) 341, 405
Eco53kI GAGCTC 1 cut(s) 339
Eco91I GGTNACC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 339
EcoO65I GGTNACC 1 cut(s) 442
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 438
EcoT38I GRGCYC 2 cut(s) 341, 405
FaeI CATG 1 cut(s) 137
FaiI YATR 9 cut(s) 46, 48, 63, 86, 135, 141, 179, 181, 300
FatI CATG 1 cut(s) 133
Fnu4HI GCNGC 3 cut(s) 106, 109, 453
FokI GGATG 2 cut(s) 292, 419
FriOI GRGCYC 2 cut(s) 341, 405
Fsp4HI GCNGC 3 cut(s) 106, 109, 453
FspBI CTAG 1 cut(s) 538
GluI GCNGC 3 cut(s) 106, 109, 453
HapII CCGG 1 cut(s) 408
HgaI GACGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 1 cut(s) 137
HinfI GANTC 6 cut(s) 79, 149, 207, 491, 497, 503
HpaII CCGG 1 cut(s) 408
HphI GGTGA 3 cut(s) 26, 88, 176
Hpy188I TCNGA 5 cut(s) 263, 370, 490, 496, 502
Hpy99I CGWCG 2 cut(s) 416, 419
HpyAV CCTTC 3 cut(s) 47, 339, 355
HpyCH4III ACNGT 2 cut(s) 99, 353
HpyCH4V TGCA 2 cut(s) 190, 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 345
HpyF3I CTNAG 3 cut(s) 122, 340, 420
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 1 cut(s) 137
KroI GCCGGC 1 cut(s) 407
KroNI GCCGGC 1 cut(s) 409
LguI GCTCTTC 3 cut(s) 329, 369, 462
LmnI GCTCC 1 cut(s) 534
LpnPI CCDG 8 cut(s) 159, 168, 178, 200, 336, 421, 425, 452
Lsp1109I GCAGC 2 cut(s) 95, 117
LweI GCATC 1 cut(s) 260
MaeI CTAG 1 cut(s) 538
MaeIII GTNAC 2 cut(s) 76, 442
MbiI CCGCTC 1 cut(s) 455
MboII GAAGA 3 cut(s) 346, 386, 449
MhlI GDGCHC 2 cut(s) 341, 405
MluCI AATT 5 cut(s) 242, 257, 276, 396, 475
MlyI GAGTC 3 cut(s) 73, 201, 497
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 7 cut(s) 313, 349, 376, 437, 446, 457, 474
MreI CGCCGGCG 1 cut(s) 407
MroNI GCCGGC 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 1 cut(s) 345
NaeI GCCGGC 1 cut(s) 409
NgoMIV GCCGGC 1 cut(s) 407
NlaIII CATG 1 cut(s) 137
NmuCI GTSAC 1 cut(s) 76
NspV TTCGAA 2 cut(s) 332, 473
PaqCI CACCTGC 1 cut(s) 195
PciSI GCTCTTC 3 cut(s) 329, 369, 462
PcsI WCGNNNNNNNCGW 2 cut(s) 411, 479
PdiI GCCGGC 1 cut(s) 409
PfeI GAWTC 3 cut(s) 149, 491, 497
PflFI GACNNNGTC 1 cut(s) 135
PkrI GCNGC 3 cut(s) 107, 110, 454
PleI GAGTC 3 cut(s) 73, 201, 497
PpsI GAGTC 3 cut(s) 73, 201, 497
Psp124BI GAGCTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 438
PsyI GACNNNGTC 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 108
SacI GAGCTC 1 cut(s) 341
SapI GCTCTTC 3 cut(s) 329, 369, 462
SatI GCNGC 3 cut(s) 106, 109, 453
SchI GAGTC 3 cut(s) 73, 201, 497
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 2 cut(s) 341, 405
SfaNI GCATC 1 cut(s) 260
SfuI TTCGAA 2 cut(s) 332, 473
SgrAI CRCCGGYG 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 5 cut(s) 242, 257, 276, 396, 475
SsiI CCGC 2 cut(s) 450, 453
SspMI CTAG 1 cut(s) 538
SstI GAGCTC 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 99, 353
TaqI TCGA 4 cut(s) 101, 130, 332, 473
TasI AATT 5 cut(s) 242, 257, 276, 396, 475
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 3 cut(s) 149, 491, 497
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 2 cut(s) 105, 108
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 4 cut(s) 235, 300, 387, 468
TspRI CASTG 1 cut(s) 358
Tth111I GACNNNGTC 1 cut(s) 135
XapI RAATTY 2 cut(s) 276, 475
XspI CTAG 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.