Rroxscaffold_1G00029840

phenylalanine--tRNA ligase alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005532
Physical Location & Seq
Reverse (-)
38832613 .. 38835068
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029840.1

Sequence Viewer

Length: 870 bp
ATGGCCGAAGCTCGAGAATCCGATCAAAGAGTGAAGAAGAAGAAGAAGAGGAACACTATGGAAGCCGAAGCTCAAGAATCGAGCCCCCAAAACGACGCCGTCATCACCTCCTTCCGCGAGATCACCTCCGCCTCCAAACAAGAAGCCCTCTTCTTCCTCGAATTCCACAATTACGACCTCGACGCCTCCGTTTCCACTTTCTTGGACAACAAAAACGCCGCCGCCGTCACCGTCACCAACCCGGTCCGGCCCTCCCACTCACTTTCGCCGTCTCCGTCACACTCGCCCGAGTACTCGCCCTCCCGTTGCCCACGCGCTCTCGCGGACCAAGCTAAACTCAACCACGACGACATCGCCAATGCTATTAAGAGTCTCACCGGTCACGGATACGTTGACTCTCGGGGTTTCGGGTGTGCCATTCGCTTGGTGGAGGAACCGGATCGGTATGGGGACTCGGTGATTTCAAAAATCGAGGAGGAATACCACGACGGGATGATGCTCACTTTCTCTGTGTTTCCATCACCAAAAGTCTCCGATACGGTGGTTGAGCCCTATAATGCTACCTTGTCCGTGCACCGGCTTGCGAGAATGCGGATGAGTTCCCTGGGCAACTCAACTCGACCTCGAAAGTTGGCTGTCAATCTCATTCCATTCCCCGTCTCCACTTTTTCATTCCATTCCTGGTGGGATGTTTTAATGCAGAAAAAGTTTGATGCATCGGTTTTCAAAATAGCTTGCGCCCAGGCTGCAAAGAATAAATGGGTGGACTTTGGAAAGCAACTAGTGACCGGAAAGATCCAACATGTGGATAATGACAAGGTCCGAGACTTTGCTTCTACGGATACGGGACGCAACAAAACAACAAATTAA

Protein Analysis

289

Amino Acids

32.29

Weight (kDa)

8.64

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_4 PF14555 32 - 71 9.7e-08 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 805
AccII CGCG 3 cut(s) 117, 315, 323
AciI CCGC 6 cut(s) 115, 129, 219, 222, 323, 592
AclWI GGATC 2 cut(s) 447, 790
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 161
AcyI GRCGYC 2 cut(s) 96, 183
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 576, 805
AflIII ACRYGT 1 cut(s) 802
AgeI ACCGGT 1 cut(s) 377
AgsI TTSAA 2 cut(s) 465, 727
AhlI ACTAGT 1 cut(s) 781
AjnI CCWGG 3 cut(s) 603, 680, 741
AluBI AGCT 4 cut(s) 11, 71, 332, 734
AluI AGCT 4 cut(s) 11, 71, 332, 734
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 5 cut(s) 276, 377, 535, 664, 819
Alw44I GTGCAC 1 cut(s) 572
AlwI GGATC 2 cut(s) 447, 790
Ama87I CYCGRG 3 cut(s) 12, 287, 399
AoxI GGCC 2 cut(s) 3, 248
ApaLI GTGCAC 1 cut(s) 572
ApeKI GCWGC 1 cut(s) 746
ApoI RAATTY 1 cut(s) 161
AsiGI ACCGGT 1 cut(s) 377
AspLEI GCGC 2 cut(s) 317, 740
AspS9I GGNCC 4 cut(s) 244, 249, 325, 820
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 7 cut(s) 97, 115, 220, 226, 367, 469, 513
AvaI CYCGRG 3 cut(s) 12, 287, 399
AvaII GGWCC 3 cut(s) 244, 325, 820
BaeGI GKGCMC 1 cut(s) 576
BaeI ACNNNNGTAYC 2 cut(s) 528, 561
BanII GRGCYC 2 cut(s) 86, 552
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 1 cut(s) 733
BccI CCATC 1 cut(s) 526
BceAI ACGGC 3 cut(s) 83, 209, 253
BciT130I CCWGG 3 cut(s) 605, 682, 743
BciVI GTATCC 2 cut(s) 380, 835
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 5 cut(s) 276, 377, 535, 664, 819
BcuI ACTAGT 1 cut(s) 781
BfaI CTAG 1 cut(s) 782
BfuI GTATCC 2 cut(s) 380, 835
BisI GCNGC 3 cut(s) 219, 222, 747
BlsI GCNGC 3 cut(s) 220, 223, 748
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 4 cut(s) 242, 605, 682, 743
Bme18I GGWCC 3 cut(s) 244, 325, 820
BmeT110I CYCGRG 3 cut(s) 12, 287, 399
BmgT120I GGNCC 4 cut(s) 244, 249, 325, 820
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 4 cut(s) 242, 605, 682, 743
BmsI GCATC 3 cut(s) 486, 703, 725
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpuEI CTTGAG 1 cut(s) 57
BpuMI CCSGG 1 cut(s) 242
BsaHI GRCGYC 2 cut(s) 96, 183
BsaJI CCNNGG 3 cut(s) 603, 604, 741
BsaWI WCCGGW 3 cut(s) 377, 436, 788
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 576, 805
Bse118I RCCGGY 2 cut(s) 377, 576
BseBI CCWGG 3 cut(s) 605, 682, 743
BseDI CCNNGG 3 cut(s) 603, 604, 741
BseGI GGATG 3 cut(s) 498, 600, 694
BseLI CCNNNNNNNGG 2 cut(s) 576, 805
BseRI GAGGAG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 576
BseXI GCAGC 1 cut(s) 733
Bsh1236I CGCG 3 cut(s) 117, 315, 323
BshFI GGCC 2 cut(s) 5, 250
BshTI ACCGGT 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 576
BsiHKCI CYCGRG 3 cut(s) 12, 287, 399
BsiSI CCGG 6 cut(s) 242, 247, 378, 437, 577, 789
BslFI GGGAC 2 cut(s) 464, 861
BslI CCNNNNNNNGG 2 cut(s) 576, 805
BsmAI GTCTC 5 cut(s) 276, 377, 535, 664, 819
BsmBI CGTCTC 2 cut(s) 276, 664
BsmFI GGGAC 2 cut(s) 464, 861
BsmI GAATGC 1 cut(s) 594
BsnI GGCC 2 cut(s) 5, 250
BsoBI CYCGRG 3 cut(s) 12, 287, 399
Bsp1286I GDGCHC 3 cut(s) 86, 552, 576
Bsp143I GATC 4 cut(s) 22, 120, 439, 795
BspACI CCGC 6 cut(s) 115, 129, 219, 222, 323, 592
BspANI GGCC 2 cut(s) 5, 250
BspFNI CGCG 3 cut(s) 117, 315, 323
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 2 cut(s) 447, 790
BsrFI RCCGGY 2 cut(s) 377, 576
BssAI RCCGGY 2 cut(s) 377, 576
BssECI CCNNGG 3 cut(s) 603, 604, 741
BssMI GATC 4 cut(s) 22, 120, 439, 795
BssNI GRCGYC 2 cut(s) 96, 183
Bst2UI CCWGG 3 cut(s) 605, 682, 743
Bst4CI ACNGT 2 cut(s) 232, 541
Bst6I CTCTTC 2 cut(s) 41, 155
BstACI GRCGYC 2 cut(s) 96, 183
BstC8I GCNNGC 2 cut(s) 582, 736
BstF5I GGATG 3 cut(s) 498, 600, 694
BstFNI CGCG 3 cut(s) 117, 315, 323
BstHHI GCGC 2 cut(s) 317, 740
BstKTI GATC 4 cut(s) 25, 123, 442, 798
BstMAI GTCTC 5 cut(s) 276, 377, 535, 664, 819
BstMBI GATC 4 cut(s) 22, 120, 439, 795
BstMWI GCNNNNNNNGC 2 cut(s) 329, 746
BstNI CCWGG 3 cut(s) 605, 682, 743
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 240, 603, 680, 741
BstSLI GKGCMC 1 cut(s) 576
BstUI CGCG 3 cut(s) 117, 315, 323
BstV1I GCAGC 1 cut(s) 733
BstX2I RGATCY 1 cut(s) 795
BstXI CCANNNNNNTGG 2 cut(s) 202, 424
BstYI RGATCY 1 cut(s) 795
BsuI GTATCC 2 cut(s) 380, 835
BsuRI GGCC 2 cut(s) 5, 250
BtgZI GCGATG 1 cut(s) 337
BtsCI GGATG 3 cut(s) 498, 600, 694
Cac8I GCNNGC 2 cut(s) 582, 736
CfoI GCGC 2 cut(s) 317, 740
Cfr10I RCCGGY 2 cut(s) 377, 576
Cfr13I GGNCC 4 cut(s) 244, 249, 325, 820
CpoI CGGWCCG 1 cut(s) 244
CseI GACGC 3 cut(s) 104, 191, 858
Csp6I GTAC 1 cut(s) 292
CspAI ACCGGT 1 cut(s) 377
CspI CGGWCCG 1 cut(s) 244
CviAII CATG 1 cut(s) 803
CviQI GTAC 1 cut(s) 292
DpnI GATC 4 cut(s) 24, 122, 441, 797
DpnII GATC 4 cut(s) 22, 120, 439, 795
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 41, 155
EarI CTCTTC 2 cut(s) 41, 155
EciI GGCGGA 1 cut(s) 118
Eco24I GRGCYC 2 cut(s) 86, 552
Eco47I GGWCC 3 cut(s) 244, 325, 820
Eco88I CYCGRG 3 cut(s) 12, 287, 399
EcoRI GAATTC 1 cut(s) 161
EcoRII CCWGG 3 cut(s) 603, 680, 741
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 2 cut(s) 86, 552
Esp3I CGTCTC 2 cut(s) 276, 664
FaeI CATG 1 cut(s) 806
FaiI YATR 4 cut(s) 59, 447, 555, 804
FaqI GGGAC 2 cut(s) 464, 861
FatI CATG 1 cut(s) 802
Fnu4HI GCNGC 3 cut(s) 219, 222, 747
FokI GGATG 3 cut(s) 505, 607, 701
FriOI GRGCYC 2 cut(s) 86, 552
Fsp4HI GCNGC 3 cut(s) 219, 222, 747
FspBI CTAG 1 cut(s) 782
GlaI GCGC 2 cut(s) 316, 739
GluI GCNGC 3 cut(s) 219, 222, 747
HaeIII GGCC 2 cut(s) 5, 250
HapII CCGG 6 cut(s) 242, 247, 378, 437, 577, 789
HgaI GACGC 3 cut(s) 104, 191, 858
HhaI GCGC 2 cut(s) 317, 740
Hin1I GRCGYC 2 cut(s) 96, 183
Hin1II CATG 1 cut(s) 806
Hin6I GCGC 2 cut(s) 315, 738
HinP1I GCGC 2 cut(s) 315, 738
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 5 cut(s) 17, 77, 370, 395, 452
HpaII CCGG 6 cut(s) 242, 247, 378, 437, 577, 789
HphI GGTGA 7 cut(s) 97, 115, 220, 226, 367, 469, 513
Hpy166II GTNNAC 3 cut(s) 394, 574, 766
Hpy188I TCNGA 3 cut(s) 22, 535, 824
Hpy188III TCNNGA 2 cut(s) 14, 74
Hpy8I GTNNAC 3 cut(s) 394, 574, 766
Hpy99I CGWCG 4 cut(s) 98, 185, 350, 491
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 2 cut(s) 232, 541
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 4 cut(s) 574, 700, 716, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 746
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 2 cut(s) 96, 183
Hsp92II CATG 1 cut(s) 806
HspAI GCGC 2 cut(s) 315, 738
Kzo9I GATC 4 cut(s) 22, 120, 439, 795
Lsp1109I GCAGC 1 cut(s) 733
LweI GCATC 3 cut(s) 486, 703, 725
MaeI CTAG 1 cut(s) 782
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 5 cut(s) 226, 232, 276, 380, 784
MalI GATC 4 cut(s) 24, 122, 441, 797
MboI GATC 4 cut(s) 22, 120, 439, 795
MboII GAAGA 7 cut(s) 46, 49, 52, 55, 58, 142, 145
MflI RGATCY 1 cut(s) 795
MhlI GDGCHC 3 cut(s) 86, 552, 576
MluCI AATT 3 cut(s) 161, 169, 865
MlyI GAGTC 3 cut(s) 379, 389, 446
MmeI TCCRAC 1 cut(s) 823
Mph1103I ATGCAT 1 cut(s) 718
MseI TTAA 3 cut(s) 366, 695, 868
MspI CCGG 6 cut(s) 242, 247, 378, 437, 577, 789
MspR9I CCNGG 4 cut(s) 242, 605, 682, 743
Mva1269I GAATGC 1 cut(s) 594
MvaI CCWGG 3 cut(s) 605, 682, 743
MvnI CGCG 3 cut(s) 117, 315, 323
MwoI GCNNNNNNNGC 2 cut(s) 329, 746
NciI CCSGG 1 cut(s) 242
NdeII GATC 4 cut(s) 22, 120, 439, 795
NlaIII CATG 1 cut(s) 806
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 5 cut(s) 226, 232, 276, 380, 784
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 806
PaeR7I CTCGAG 1 cut(s) 12
PasI CCCWGGG 1 cut(s) 604
PciI ACATGT 1 cut(s) 802
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 222, 272
PctI GAATGC 1 cut(s) 594
PfeI GAWTC 2 cut(s) 17, 77
PflFI GACNNNGTC 2 cut(s) 98, 818
PflMI CCANNNNNTGG 1 cut(s) 805
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 3 cut(s) 220, 223, 748
PleI GAGTC 3 cut(s) 378, 389, 446
PpsI GAGTC 3 cut(s) 378, 389, 446
PscI ACATGT 1 cut(s) 802
Psp6I CCWGG 3 cut(s) 603, 680, 741
PspGI CCWGG 3 cut(s) 603, 680, 741
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 4 cut(s) 244, 249, 325, 820
PsuI RGATCY 1 cut(s) 795
PsyI GACNNNGTC 2 cut(s) 98, 818
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
Rsr2I CGGWCCG 1 cut(s) 244
RsrII CGGWCCG 1 cut(s) 244
SaqAI TTAA 3 cut(s) 366, 695, 868
SatI GCNGC 3 cut(s) 219, 222, 747
Sau3AI GATC 4 cut(s) 22, 120, 439, 795
Sau96I GGNCC 4 cut(s) 244, 249, 325, 820
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 3 cut(s) 379, 389, 446
ScrFI CCNGG 4 cut(s) 242, 605, 682, 743
SduI GDGCHC 3 cut(s) 86, 552, 576
SfaNI GCATC 3 cut(s) 486, 703, 725
Sfr274I CTCGAG 1 cut(s) 12
SinI GGWCC 3 cut(s) 244, 325, 820
SlaI CTCGAG 1 cut(s) 12
SmlI CTYRAG 2 cut(s) 12, 72
SmoI CTYRAG 2 cut(s) 12, 72
SpeI ACTAGT 1 cut(s) 781
Sse9I AATT 3 cut(s) 161, 169, 865
SsiI CCGC 6 cut(s) 115, 129, 219, 222, 323, 592
SspMI CTAG 1 cut(s) 782
StyD4I CCNGG 4 cut(s) 240, 603, 680, 741
TaaI ACNGT 2 cut(s) 232, 541
TaiI ACGT 1 cut(s) 393
TaqI TCGA 7 cut(s) 13, 80, 159, 180, 471, 619, 625
TasI AATT 3 cut(s) 161, 169, 865
TatI WGTACW 1 cut(s) 291
TauI GCSGC 2 cut(s) 221, 224
TfiI GAWTC 2 cut(s) 17, 77
Tru1I TTAA 3 cut(s) 366, 695, 868
Tru9I TTAA 3 cut(s) 366, 695, 868
TseFI GTSAC 5 cut(s) 226, 232, 276, 380, 784
TseI GCWGC 1 cut(s) 746
Tsp45I GTSAC 5 cut(s) 226, 232, 276, 380, 784
TspDTI ATGAA 1 cut(s) 660
TspGWI ACGGA 5 cut(s) 178, 264, 399, 559, 854
Tth111I GACNNNGTC 2 cut(s) 98, 818
Van91I CCANNNNNTGG 1 cut(s) 805
VneI GTGCAC 1 cut(s) 572
VpaK11BI GGWCC 3 cut(s) 244, 325, 820
XapI RAATTY 1 cut(s) 161
XceI RCATGY 1 cut(s) 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 12
XspI CTAG 1 cut(s) 782
ZrmI AGTACT 1 cut(s) 293
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.