Rh4CG081600

Zinc finger HIT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005532
Physical Location & Seq
Reverse (-)
14510276 .. 14512524
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG081600.1

Sequence Viewer

Length: 936 bp
ATGCTATTAAGAGTCTCACCGGTCACAGATACGTTGACTCTCAGATTAATCCAATTTCCTATTCCTGGATTGTGTAATGGTGTGTTGATTACTTCTTGCTACTTGTCGATTTTGCATGAGATTACAAAGGAGACATGGTTGCTCACCGAGGAAGGGAAGACATATGCTGTTCATGGAACGCCAGAAGTGTGGCTCTTCCTGTCCATACCGCAAGAGGGCATCACAAAAGATGAATTGCAGAAAAAGTTTGATGCATCGGTTTTCAAAATAGCTTGCACCCAGGCTGCAAAGAATAAATGGGTGGACTTTGGAAAGCAACTAGTGACCGGAAAGCTAGGGCAATTAGGGCCGAATGACCAAACGAAGCAGAAGATGATGGACATACTGAAAAGGTTTCACTCTGAAGATGAAGCTGATGGCATGGATGAGGATGTGGATGCTGATTCTGCACTGTCTGAGGAGACTATTAAAAAAGTGCTGTCTGGAGGTGAAGTTAGTTTTGATGATCTATCTGCAGAAGAGAAGAAACTATTCCAAAGAGCTGTTGCTTCTGGAGAACTGAGCAAGATGATTAAGCCTTGGGATCCTTGGTGGTTGAAGCCTTCTGCCAGGACAATATCCTTCAGCAAAGAAGGAACCCAACTTATCCAACCGCTTGCCAAAGAGGACGAACCAATGTCACCAGAAGAGGATCAAGAAAGCAGTCAAGCAAGTGAGATTCCACCAGGTCCTGAGACACCACTGCCTCCCCTCAGCAACCTTAGTTCCGCGAAGCCATCGCCATTCTTAGCTATCCAACATGTGGATAATGACAAGGTCCAGACTTTGCTTCTACGGATACAGGACGCAACAAAACAACAAATAGCAGCAACAAAACAAGGGAAACATAGAGAATCTGAAAGAATGGCAAGCAAGGACCTGGGAAACTTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHQ1 zf-HIT

Protein Analysis

311

Amino Acids

34.58

Weight (kDa)

5.09

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PheRS_DBD3 PF18553 60 - 106 3.2e-09 PheRS DNA binding domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 802
AccII CGCG 1 cut(s) 770
AciI CCGC 3 cut(s) 209, 653, 768
AclWI GGATC 3 cut(s) 578, 591, 699
AcuI CTGAAG 2 cut(s) 423, 607
AfiI CCNNNNNNNGG 4 cut(s) 65, 153, 215, 802
AflIII ACRYGT 1 cut(s) 799
AgeI ACCGGT 1 cut(s) 19
AgsI TTSAA 2 cut(s) 265, 598
AhlI ACTAGT 1 cut(s) 319
AjnI CCWGG 5 cut(s) 64, 279, 608, 724, 918
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
AluBI AGCT 5 cut(s) 272, 334, 413, 542, 791
AluI AGCT 5 cut(s) 272, 334, 413, 542, 791
Alw26I GTCTC 4 cut(s) 19, 125, 455, 728
AlwI GGATC 3 cut(s) 578, 591, 699
AlwNI CAGNNNCTG 1 cut(s) 731
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 284, 866
AseI ATTAAT 1 cut(s) 47
AsiGI ACCGGT 1 cut(s) 19
Asp700I GAANNNNTTC 1 cut(s) 530
AspS9I GGNCC 4 cut(s) 347, 728, 817, 916
AsuHPI GGTGA 4 cut(s) 9, 136, 500, 672
AvaII GGWCC 3 cut(s) 728, 817, 916
BamHI GGATCC 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 164
BbvCI CCTCAGC 1 cut(s) 752
BbvI GCAGC 2 cut(s) 271, 878
BccI CCATC 3 cut(s) 370, 410, 784
BciT130I CCWGG 5 cut(s) 66, 281, 610, 726, 920
BciVI GTATCC 1 cut(s) 831
BcoDI GTCTC 4 cut(s) 19, 125, 455, 728
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 2 cut(s) 320, 335
BfmI CTRYAG 1 cut(s) 513
BfuI GTATCC 1 cut(s) 831
BisI GCNGC 2 cut(s) 285, 867
BlsI GCNGC 2 cut(s) 286, 868
Bme1390I CCNGG 5 cut(s) 66, 281, 610, 726, 920
Bme18I GGWCC 3 cut(s) 728, 817, 916
BmgT120I GGNCC 4 cut(s) 347, 728, 817, 916
BmiI GGNNCC 2 cut(s) 585, 637
BmrFI CCNGG 5 cut(s) 66, 281, 610, 726, 920
BmsI GCATC 4 cut(s) 228, 241, 263, 427
BpiI GAAGAC 1 cut(s) 164
BpmI CTGGAG 2 cut(s) 504, 573
Bpu10I CCTNAGC 1 cut(s) 752
BsaJI CCNNGG 5 cut(s) 147, 279, 578, 587, 919
BsaWI WCCGGW 2 cut(s) 19, 326
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 477, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 153, 215, 802
Bse118I RCCGGY 1 cut(s) 19
BseBI CCWGG 5 cut(s) 66, 281, 610, 726, 920
BseDI CCNNGG 5 cut(s) 147, 279, 578, 587, 919
BseGI GGATG 3 cut(s) 430, 436, 442
BseLI CCNNNNNNNGG 4 cut(s) 65, 153, 215, 802
BseMII CTCAG 5 cut(s) 55, 447, 551, 723, 766
BseRI GAGGAG 1 cut(s) 473
BseXI GCAGC 2 cut(s) 271, 878
BsgI GTGCAG 1 cut(s) 432
Bsh1236I CGCG 1 cut(s) 770
BshFI GGCC 1 cut(s) 349
BshTI ACCGGT 1 cut(s) 19
BsiSI CCGG 2 cut(s) 20, 327
BslI CCNNNNNNNGG 4 cut(s) 65, 153, 215, 802
BsmAI GTCTC 4 cut(s) 19, 125, 455, 728
BsnI GGCC 1 cut(s) 349
Bsp143I GATC 3 cut(s) 505, 583, 691
BspACI CCGC 3 cut(s) 209, 653, 768
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 5 cut(s) 54, 448, 552, 724, 765
BspFNI CGCG 1 cut(s) 770
BspLI GGNNCC 2 cut(s) 585, 637
BspMAI CTGCAG 1 cut(s) 517
BspPI GGATC 3 cut(s) 578, 591, 699
BspQI GCTCTTC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 19
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 5 cut(s) 147, 279, 578, 587, 919
BssMI GATC 3 cut(s) 505, 583, 691
BssT1I CCWWGG 2 cut(s) 578, 587
Bst2UI CCWGG 5 cut(s) 66, 281, 610, 726, 920
Bst4CI ACNGT 1 cut(s) 453
Bst6I CTCTTC 3 cut(s) 200, 513, 681
BstC8I GCNNGC 3 cut(s) 274, 657, 910
BstDEI CTNAG 7 cut(s) 41, 456, 560, 732, 752, 761, 787
BstF5I GGATG 3 cut(s) 430, 436, 442
BstFNI CGCG 1 cut(s) 770
BstKTI GATC 3 cut(s) 508, 586, 694
BstMAI GTCTC 4 cut(s) 19, 125, 455, 728
BstMBI GATC 3 cut(s) 505, 583, 691
BstMWI GCNNNNNNNGC 2 cut(s) 346, 446
BstNI CCWGG 5 cut(s) 66, 281, 610, 726, 920
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 5 cut(s) 64, 279, 608, 724, 918
BstSFI CTRYAG 1 cut(s) 513
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 2 cut(s) 271, 878
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 583
BstXI CCANNNNNNTGG 1 cut(s) 189
BstYI RGATCY 1 cut(s) 583
BsuI GTATCC 1 cut(s) 831
BsuRI GGCC 1 cut(s) 349
BtgZI GCGATG 1 cut(s) 762
BtsCI GGATG 3 cut(s) 430, 436, 442
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 2 cut(s) 449, 740
Cac8I GCNNGC 3 cut(s) 274, 657, 910
CaiI CAGNNNCTG 1 cut(s) 731
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 4 cut(s) 347, 728, 817, 916
CseI GACGC 1 cut(s) 854
CsiI ACCWGGT 1 cut(s) 724
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 5 cut(s) 116, 135, 173, 421, 800
DdeI CTNAG 7 cut(s) 41, 456, 560, 732, 752, 761, 787
DpnI GATC 3 cut(s) 507, 585, 693
DpnII GATC 3 cut(s) 505, 583, 691
Eam1104I CTCTTC 3 cut(s) 200, 513, 681
EarI CTCTTC 3 cut(s) 200, 513, 681
Eco130I CCWWGG 2 cut(s) 578, 587
Eco47I GGWCC 3 cut(s) 728, 817, 916
Eco57I CTGAAG 2 cut(s) 423, 607
EcoO109I RGGNCCY 2 cut(s) 728, 916
EcoRII CCWGG 5 cut(s) 64, 279, 608, 724, 918
EcoT14I CCWWGG 2 cut(s) 578, 587
EcoT22I ATGCAT 1 cut(s) 256
ErhI CCWWGG 2 cut(s) 578, 587
FaeI CATG 5 cut(s) 119, 138, 176, 424, 803
FatI CATG 5 cut(s) 115, 134, 172, 420, 799
FauNDI CATATG 1 cut(s) 163
Fnu4HI GCNGC 2 cut(s) 285, 867
FokI GGATG 3 cut(s) 437, 443, 449
Fsp4HI GCNGC 2 cut(s) 285, 867
FspBI CTAG 2 cut(s) 320, 335
GluI GCNGC 2 cut(s) 285, 867
GsuI CTGGAG 2 cut(s) 504, 573
HaeIII GGCC 1 cut(s) 349
HapII CCGG 2 cut(s) 20, 327
HgaI GACGC 1 cut(s) 854
Hin1II CATG 5 cut(s) 119, 138, 176, 424, 803
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 5 cut(s) 12, 37, 443, 718, 893
HpaII CCGG 2 cut(s) 20, 327
HphI GGTGA 4 cut(s) 9, 136, 500, 672
Hpy166II GTNNAC 2 cut(s) 36, 304
Hpy188I TCNGA 4 cut(s) 44, 403, 457, 898
Hpy188III TCNNGA 5 cut(s) 483, 552, 695, 731, 820
Hpy8I GTNNAC 2 cut(s) 36, 304
HpyAV CCTTC 4 cut(s) 146, 612, 626, 631
HpyCH4III ACNGT 1 cut(s) 453
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 7 cut(s) 115, 238, 254, 276, 287, 449, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 346, 446
HpyF3I CTNAG 7 cut(s) 41, 456, 560, 732, 752, 761, 787
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 5 cut(s) 119, 138, 176, 424, 803
Kzo9I GATC 3 cut(s) 505, 583, 691
LguI GCTCTTC 1 cut(s) 200
Lsp1109I GCAGC 2 cut(s) 271, 878
LweI GCATC 4 cut(s) 228, 241, 263, 427
MabI ACCWGGT 1 cut(s) 724
MaeI CTAG 2 cut(s) 320, 335
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 22, 322, 678
MalI GATC 3 cut(s) 507, 585, 693
MboI GATC 3 cut(s) 505, 583, 691
MboII GAAGA 7 cut(s) 169, 187, 382, 416, 530, 535, 698
MflI RGATCY 1 cut(s) 583
MluCI AATT 4 cut(s) 53, 233, 341, 931
MlyI GAGTC 2 cut(s) 21, 31
MmeI TCCRAC 2 cut(s) 673, 820
MnlI CCTC 9 cut(s) 142, 208, 421, 451, 479, 658, 682, 756, 761
Mph1103I ATGCAT 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 5 cut(s) 8, 47, 468, 573, 929
MspI CCGG 2 cut(s) 20, 327
MspR9I CCNGG 5 cut(s) 66, 281, 610, 726, 920
MvaI CCWGG 5 cut(s) 66, 281, 610, 726, 920
MvnI CGCG 1 cut(s) 770
MwoI GCNNNNNNNGC 2 cut(s) 346, 446
NdeI CATATG 1 cut(s) 163
NdeII GATC 3 cut(s) 505, 583, 691
NlaIII CATG 5 cut(s) 119, 138, 176, 424, 803
NlaIV GGNNCC 2 cut(s) 585, 637
NmuCI GTSAC 3 cut(s) 22, 322, 678
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 1 cut(s) 803
PciI ACATGT 1 cut(s) 799
PciSI GCTCTTC 1 cut(s) 200
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 3 cut(s) 443, 718, 893
PflFI GACNNNGTC 1 cut(s) 815
PflMI CCANNNNNTGG 1 cut(s) 802
PfoI TCCNGGA 1 cut(s) 64
PinAI ACCGGT 1 cut(s) 19
PkrI GCNGC 2 cut(s) 286, 868
PleI GAGTC 2 cut(s) 20, 31
PpsI GAGTC 2 cut(s) 20, 31
PpuMI RGGWCCY 2 cut(s) 728, 916
PscI ACATGT 1 cut(s) 799
PshBI ATTAAT 1 cut(s) 47
Psp5II RGGWCCY 2 cut(s) 728, 916
Psp6I CCWGG 5 cut(s) 64, 279, 608, 724, 918
PspGI CCWGG 5 cut(s) 64, 279, 608, 724, 918
PspN4I GGNNCC 2 cut(s) 585, 637
PspPI GGNCC 4 cut(s) 347, 728, 817, 916
PspPPI RGGWCCY 2 cut(s) 728, 916
PstI CTGCAG 1 cut(s) 517
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 1 cut(s) 583
PsyI GACNNNGTC 1 cut(s) 815
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 5 cut(s) 8, 47, 468, 573, 929
SatI GCNGC 2 cut(s) 285, 867
Sau3AI GATC 3 cut(s) 505, 583, 691
Sau96I GGNCC 4 cut(s) 347, 728, 817, 916
SchI GAGTC 2 cut(s) 21, 31
ScrFI CCNGG 5 cut(s) 66, 281, 610, 726, 920
SexAI ACCWGGT 1 cut(s) 724
SfaNI GCATC 4 cut(s) 228, 241, 263, 427
SfcI CTRYAG 1 cut(s) 513
SinI GGWCC 3 cut(s) 728, 817, 916
SpeI ACTAGT 1 cut(s) 319
Sse9I AATT 4 cut(s) 53, 233, 341, 931
SsiI CCGC 3 cut(s) 209, 653, 768
SspMI CTAG 2 cut(s) 320, 335
StyD4I CCNGG 5 cut(s) 64, 279, 608, 724, 918
StyI CCWWGG 2 cut(s) 578, 587
TaaI ACNGT 1 cut(s) 453
TaiI ACGT 1 cut(s) 35
TaqI TCGA 1 cut(s) 107
TasI AATT 4 cut(s) 53, 233, 341, 931
TfiI GAWTC 3 cut(s) 443, 718, 893
Tru1I TTAA 5 cut(s) 8, 47, 468, 573, 929
Tru9I TTAA 5 cut(s) 8, 47, 468, 573, 929
TscAI CASTG 2 cut(s) 456, 747
TseFI GTSAC 3 cut(s) 22, 322, 678
TseI GCWGC 2 cut(s) 284, 866
Tsp45I GTSAC 3 cut(s) 22, 322, 678
TspDTI ATGAA 3 cut(s) 161, 246, 423
TspGWI ACGGA 1 cut(s) 850
TspRI CASTG 2 cut(s) 456, 747
Tth111I GACNNNGTC 1 cut(s) 815
Van91I CCANNNNNTGG 1 cut(s) 802
VpaK11BI GGWCC 3 cut(s) 728, 817, 916
VspI ATTAAT 1 cut(s) 47
XceI RCATGY 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 2 cut(s) 320, 335
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.