Rh4AG076700

Zinc finger HIT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005532
Physical Location & Seq
Reverse (-)
15563642 .. 15565081
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG076700.1

Sequence Viewer

Length: 564 bp
ATGATGGACATACTGAAAAGGTTTCACTCTGAAGATGAAGCTGATGGCATGGATGAGGATGTGGATGCTGATTCTGCACTGTCTGAGGAGACTATTAAAAAAGTGCTGTCTGGAGGTGAAGTTAGTTTTGATGATCTATCTGCAGAAGAGAAGAAACTATTCCAAAGAGCTGTTGCTTCTGGAGAACTGAGCAAGATGATTAAGCCTTGGGATCCTTGGTGGTTGAAGCCTTCTGCCAGGACAATATCCTTCAGCAAAGAAGGAACCCAACTTATCCAACCGCTTGCCAAAGAGGACGAACCAATGTCACCAGAAGAGGATCAAGAAAGCAGTCAAGCAAGTGAGATTCCACCAGGTCCTGAGACACCACTGCCTCCCCTCAGCAACCTTAGTTCCGCGAAGCCATCGCCATTCTTAGCTATCCAACATGTGGATAATGACAAGGTCCAGACTTTGCTTCTACGGATACAGGACGCAACAAAACAACAAATAGCAGCAACAAAACAAGGGAAACATAGAGAATCTGAAAGAATGGCAAGCAAGGACCTGGGAAACTTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHQ1 zf-HIT

Protein Analysis

187

Amino Acids

20.73

Weight (kDa)

4.68

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 430
AccII CGCG 1 cut(s) 398
AciI CCGC 2 cut(s) 281, 396
AclWI GGATC 3 cut(s) 206, 219, 327
AcuI CTGAAG 2 cut(s) 51, 235
AfiI CCNNNNNNNGG 1 cut(s) 430
AflIII ACRYGT 1 cut(s) 427
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 3 cut(s) 236, 352, 546
AluBI AGCT 3 cut(s) 41, 170, 419
AluI AGCT 3 cut(s) 41, 170, 419
Alw26I GTCTC 2 cut(s) 83, 356
AlwI GGATC 3 cut(s) 206, 219, 327
AlwNI CAGNNNCTG 1 cut(s) 359
ApeKI GCWGC 1 cut(s) 494
Asp700I GAANNNNTTC 1 cut(s) 158
AspS9I GGNCC 3 cut(s) 356, 445, 544
AsuHPI GGTGA 2 cut(s) 128, 300
AvaII GGWCC 3 cut(s) 356, 445, 544
BamHI GGATCC 1 cut(s) 211
BbvCI CCTCAGC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 506
BccI CCATC 2 cut(s) 38, 412
BciT130I CCWGG 3 cut(s) 238, 354, 548
BciVI GTATCC 1 cut(s) 459
BcoDI GTCTC 2 cut(s) 83, 356
BfmI CTRYAG 1 cut(s) 141
BfuI GTATCC 1 cut(s) 459
BisI GCNGC 1 cut(s) 495
BlsI GCNGC 1 cut(s) 496
Bme1390I CCNGG 3 cut(s) 238, 354, 548
Bme18I GGWCC 3 cut(s) 356, 445, 544
BmgT120I GGNCC 3 cut(s) 356, 445, 544
BmiI GGNNCC 2 cut(s) 213, 265
BmrFI CCNGG 3 cut(s) 238, 354, 548
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 2 cut(s) 132, 201
Bpu10I CCTNAGC 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 206, 215, 547
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 430
BseBI CCWGG 3 cut(s) 238, 354, 548
BseDI CCNNGG 3 cut(s) 206, 215, 547
BseGI GGATG 3 cut(s) 58, 64, 70
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 4 cut(s) 75, 179, 351, 394
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 1 cut(s) 506
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 398
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 2 cut(s) 83, 356
Bsp143I GATC 3 cut(s) 133, 211, 319
BspACI CCGC 2 cut(s) 281, 396
BspCNI CTCAG 4 cut(s) 76, 180, 352, 393
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 2 cut(s) 213, 265
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 3 cut(s) 206, 219, 327
BssECI CCNNGG 3 cut(s) 206, 215, 547
BssMI GATC 3 cut(s) 133, 211, 319
BssT1I CCWWGG 2 cut(s) 206, 215
Bst2UI CCWGG 3 cut(s) 238, 354, 548
Bst4CI ACNGT 1 cut(s) 81
Bst6I CTCTTC 2 cut(s) 141, 309
BstC8I GCNNGC 2 cut(s) 285, 538
BstDEI CTNAG 6 cut(s) 84, 188, 360, 380, 389, 415
BstF5I GGATG 3 cut(s) 58, 64, 70
BstFNI CGCG 1 cut(s) 398
BstKTI GATC 3 cut(s) 136, 214, 322
BstMAI GTCTC 2 cut(s) 83, 356
BstMBI GATC 3 cut(s) 133, 211, 319
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 3 cut(s) 238, 354, 548
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 236, 352, 546
BstSFI CTRYAG 1 cut(s) 141
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 1 cut(s) 506
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuI GTATCC 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 3 cut(s) 58, 64, 70
BtsI GCAGTG 1 cut(s) 368
BtsIMutI CAGTG 2 cut(s) 77, 368
Cac8I GCNNGC 2 cut(s) 285, 538
CaiI CAGNNNCTG 1 cut(s) 359
Cfr13I GGNCC 3 cut(s) 356, 445, 544
CseI GACGC 1 cut(s) 482
CsiI ACCWGGT 1 cut(s) 352
CviAII CATG 2 cut(s) 49, 428
CviJI RGCY 6 cut(s) 41, 170, 205, 229, 403, 419
CviKI_1 RGCY 6 cut(s) 41, 170, 205, 229, 403, 419
DdeI CTNAG 6 cut(s) 84, 188, 360, 380, 389, 415
DpnI GATC 3 cut(s) 135, 213, 321
DpnII GATC 3 cut(s) 133, 211, 319
Eam1104I CTCTTC 2 cut(s) 141, 309
EarI CTCTTC 2 cut(s) 141, 309
Eco130I CCWWGG 2 cut(s) 206, 215
Eco47I GGWCC 3 cut(s) 356, 445, 544
Eco57I CTGAAG 2 cut(s) 51, 235
EcoO109I RGGNCCY 2 cut(s) 356, 544
EcoRII CCWGG 3 cut(s) 236, 352, 546
EcoT14I CCWWGG 2 cut(s) 206, 215
ErhI CCWWGG 2 cut(s) 206, 215
FaeI CATG 2 cut(s) 52, 431
FaiI YATR 4 cut(s) 11, 50, 429, 516
FatI CATG 2 cut(s) 48, 427
Fnu4HI GCNGC 1 cut(s) 495
FokI GGATG 3 cut(s) 65, 71, 77
Fsp4HI GCNGC 1 cut(s) 495
GluI GCNGC 1 cut(s) 495
GsuI CTGGAG 2 cut(s) 132, 201
HgaI GACGC 1 cut(s) 482
Hin1II CATG 2 cut(s) 52, 431
HinfI GANTC 3 cut(s) 71, 346, 521
HphI GGTGA 2 cut(s) 128, 300
Hpy188I TCNGA 3 cut(s) 31, 85, 526
Hpy188III TCNNGA 5 cut(s) 111, 180, 323, 359, 448
HpyAV CCTTC 3 cut(s) 240, 254, 259
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 77, 143
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 6 cut(s) 84, 188, 360, 380, 389, 415
Hsp92II CATG 2 cut(s) 52, 431
Kzo9I GATC 3 cut(s) 133, 211, 319
Lsp1109I GCAGC 1 cut(s) 506
LweI GCATC 1 cut(s) 55
MabI ACCWGGT 1 cut(s) 352
MaeIII GTNAC 1 cut(s) 306
MalI GATC 3 cut(s) 135, 213, 321
MboI GATC 3 cut(s) 133, 211, 319
MboII GAAGA 4 cut(s) 44, 158, 163, 326
MflI RGATCY 1 cut(s) 211
MluCI AATT 1 cut(s) 559
MmeI TCCRAC 2 cut(s) 301, 448
MnlI CCTC 7 cut(s) 49, 79, 107, 286, 310, 384, 389
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 3 cut(s) 96, 201, 557
MspR9I CCNGG 3 cut(s) 238, 354, 548
MvaI CCWGG 3 cut(s) 238, 354, 548
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 1 cut(s) 74
NdeII GATC 3 cut(s) 133, 211, 319
NlaIII CATG 2 cut(s) 52, 431
NlaIV GGNNCC 2 cut(s) 213, 265
NmuCI GTSAC 1 cut(s) 306
NspI RCATGY 1 cut(s) 431
PciI ACATGT 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 3 cut(s) 71, 346, 521
PflFI GACNNNGTC 1 cut(s) 443
PflMI CCANNNNNTGG 1 cut(s) 430
PkrI GCNGC 1 cut(s) 496
PpuMI RGGWCCY 2 cut(s) 356, 544
PscI ACATGT 1 cut(s) 427
Psp5II RGGWCCY 2 cut(s) 356, 544
Psp6I CCWGG 3 cut(s) 236, 352, 546
PspGI CCWGG 3 cut(s) 236, 352, 546
PspN4I GGNNCC 2 cut(s) 213, 265
PspPI GGNCC 3 cut(s) 356, 445, 544
PspPPI RGGWCCY 2 cut(s) 356, 544
PstI CTGCAG 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 211
PsyI GACNNNGTC 1 cut(s) 443
SaqAI TTAA 3 cut(s) 96, 201, 557
SatI GCNGC 1 cut(s) 495
Sau3AI GATC 3 cut(s) 133, 211, 319
Sau96I GGNCC 3 cut(s) 356, 445, 544
ScrFI CCNGG 3 cut(s) 238, 354, 548
SetI ASST 9 cut(s) 23, 43, 118, 172, 358, 390, 421, 447, 549
SexAI ACCWGGT 1 cut(s) 352
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 141
SinI GGWCC 3 cut(s) 356, 445, 544
Sse9I AATT 1 cut(s) 559
SsiI CCGC 2 cut(s) 281, 396
StyD4I CCNGG 3 cut(s) 236, 352, 546
StyI CCWWGG 2 cut(s) 206, 215
TaaI ACNGT 1 cut(s) 81
TasI AATT 1 cut(s) 559
TfiI GAWTC 3 cut(s) 71, 346, 521
Tru1I TTAA 3 cut(s) 96, 201, 557
Tru9I TTAA 3 cut(s) 96, 201, 557
TscAI CASTG 2 cut(s) 84, 375
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 51
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 2 cut(s) 84, 375
Tth111I GACNNNGTC 1 cut(s) 443
Van91I CCANNNNNTGG 1 cut(s) 430
VpaK11BI GGWCC 3 cut(s) 356, 445, 544
XceI RCATGY 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.