Rroxscaffold_1G00033800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006431
Physical Location & Seq
Reverse (-)
48944134 .. 48944475
342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00033800.1

Sequence Viewer

Length: 342 bp
ATGCGATCGAACCCAAACATCCCTCCGCTTAATCTCTCCGTCTCCGCGGCGACGGTGCGAGCCGTACATCCCTCCTCCCTCGTTCTCTTTCACCGCGCCGGCCGGAGGCACTTCATTTACACTCCTTTTCGCTCTAGCCGGACACAATTTCCTACCGGACCTCCTCCACGAGTCTTCGGCCTCGGCGGAGGCGGCTGCATTGTAGACGTCGCGGAAGAAGCCGTCGACGGTCATCGAGCCCAACTAGATCGGCGTGGGATTGGCCGTGTTAATGTAGCTGGTGGTGGTGGTGTGGTGTTTCGAGTTGAAAGGCAAGAAATCAAAGACATCGAAGCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

12.24

Weight (kDa)

11.41

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 210
AccI GTMKAC 2 cut(s) 204, 225
AccII CGCG 3 cut(s) 47, 96, 212
AciI CCGC 7 cut(s) 26, 45, 47, 94, 186, 192, 212
AcoI YGGCCR 2 cut(s) 100, 262
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 1 cut(s) 308
AluBI AGCT 1 cut(s) 278
AluI AGCT 1 cut(s) 278
Alw26I GTCTC 1 cut(s) 46
AoxI GGCC 3 cut(s) 100, 178, 262
ApeKI GCWGC 1 cut(s) 195
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 83
AvaII GGWCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 241
BauI CACGAG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 182
BceAI ACGGC 3 cut(s) 47, 206, 249
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 2 cut(s) 135, 245
BisI GCNGC 3 cut(s) 48, 193, 196
BlsI GCNGC 3 cut(s) 49, 194, 197
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 166
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 45, 181
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse118I RCCGGY 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 45, 181
BseGI GGATG 2 cut(s) 18, 67
BseLI CCNNNNNNNGG 1 cut(s) 105
BseRI GAGGAG 2 cut(s) 64, 153
BseX3I CGGCCG 1 cut(s) 100
BseXI GCAGC 1 cut(s) 182
Bsh1236I CGCG 3 cut(s) 47, 96, 212
Bsh1285I CGRYCG 2 cut(s) 8, 103
BshFI GGCC 3 cut(s) 102, 180, 264
BsiEI CGRYCG 2 cut(s) 8, 103
BsiSI CCGG 4 cut(s) 99, 103, 139, 156
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 46
BsmBI CGTCTC 1 cut(s) 46
BsnI GGCC 3 cut(s) 102, 180, 264
Bsp1286I GDGCHC 1 cut(s) 241
Bsp143I GATC 2 cut(s) 5, 247
BspACI CCGC 7 cut(s) 26, 45, 47, 94, 186, 192, 212
BspANI GGCC 3 cut(s) 102, 180, 264
BspFNI CGCG 3 cut(s) 47, 96, 212
BsrFI RCCGGY 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 45, 181
BssMI GATC 2 cut(s) 5, 247
BssNI GRCGYC 1 cut(s) 207
BssSI CACGAG 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 55, 230
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 60, 100
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 18, 67
BstFNI CGCG 3 cut(s) 47, 96, 212
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 2 cut(s) 8, 250
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 2 cut(s) 5, 247
BstMCI CGRYCG 2 cut(s) 8, 103
BstMWI GCNNNNNNNGC 2 cut(s) 192, 218
BstUI CGCG 3 cut(s) 47, 96, 212
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 1 cut(s) 166
BstZI CGGCCG 1 cut(s) 100
BsuRI GGCC 3 cut(s) 102, 180, 264
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 18, 67
Cac8I GCNNGC 2 cut(s) 60, 100
CfoI GCGC 1 cut(s) 98
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 1 cut(s) 158
Cfr42I CCGCGG 1 cut(s) 48
Csp6I GTAC 1 cut(s) 65
CviQI GTAC 1 cut(s) 65
DpnI GATC 2 cut(s) 7, 249
DpnII GATC 2 cut(s) 5, 247
EaeI YGGCCR 2 cut(s) 100, 262
EagI CGGCCG 1 cut(s) 100
EciI GGCGGA 1 cut(s) 201
EclXI CGGCCG 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 158
Eco52I CGGCCG 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 46
FblI GTMKAC 2 cut(s) 204, 225
Fnu4HI GCNGC 3 cut(s) 48, 193, 196
FokI GGATG 2 cut(s) 5, 54
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 3 cut(s) 48, 193, 196
FspBI CTAG 2 cut(s) 135, 245
GlaI GCGC 1 cut(s) 97
GluI GCNGC 3 cut(s) 48, 193, 196
HaeIII GGCC 3 cut(s) 102, 180, 264
HapII CCGG 4 cut(s) 99, 103, 139, 156
HhaI GCGC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 207
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 171
HpaII CCGG 4 cut(s) 99, 103, 139, 156
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 2 cut(s) 205, 226
Hpy8I GTNNAC 2 cut(s) 205, 226
Hpy99I CGWCG 4 cut(s) 55, 212, 227, 230
HpyCH4III ACNGT 2 cut(s) 55, 230
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 1 cut(s) 198
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 218
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 207
HspAI GCGC 1 cut(s) 96
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
KspI CCGCGG 1 cut(s) 48
Kzo9I GATC 2 cut(s) 5, 247
LpnPI CCDG 5 cut(s) 112, 116, 152, 169, 264
Lsp1109I GCAGC 1 cut(s) 182
MaeI CTAG 2 cut(s) 135, 245
MaeII ACGT 1 cut(s) 207
MalI GATC 2 cut(s) 7, 249
MboI GATC 2 cut(s) 5, 247
MboII GAAGA 2 cut(s) 166, 227
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 1 cut(s) 146
MlyI GAGTC 1 cut(s) 180
MnlI CCTC 9 cut(s) 33, 82, 85, 89, 99, 171, 174, 182, 191
MroNI GCCGGC 1 cut(s) 98
MseI TTAA 2 cut(s) 30, 270
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 4 cut(s) 99, 103, 139, 156
MvnI CGCG 3 cut(s) 47, 96, 212
MwoI GCNNNNNNNGC 2 cut(s) 192, 218
NaeI GCCGGC 1 cut(s) 100
NdeII GATC 2 cut(s) 5, 247
NgoMIV GCCGGC 1 cut(s) 98
NmeAIII GCCGAG 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 100
PkrI GCNGC 3 cut(s) 49, 194, 197
Ple19I CGATCG 1 cut(s) 8
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 158
PvuI CGATCG 1 cut(s) 8
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SacII CCGCGG 1 cut(s) 48
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 30, 270
SatI GCNGC 3 cut(s) 48, 193, 196
Sau3AI GATC 2 cut(s) 5, 247
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 180
SduI GDGCHC 1 cut(s) 241
SetI ASST 3 cut(s) 163, 210, 280
Sfr303I CCGCGG 1 cut(s) 48
SgrBI CCGCGG 1 cut(s) 48
SgrDI CGTCGACG 1 cut(s) 224
SinI GGWCC 1 cut(s) 158
Sse9I AATT 1 cut(s) 146
SsiI CCGC 7 cut(s) 26, 45, 47, 94, 186, 192, 212
SspMI CTAG 2 cut(s) 135, 245
TaaI ACNGT 2 cut(s) 55, 230
TaiI ACGT 1 cut(s) 210
TaqI TCGA 5 cut(s) 8, 225, 235, 301, 330
TasI AATT 1 cut(s) 146
TauI GCSGC 2 cut(s) 50, 195
Tru1I TTAA 2 cut(s) 30, 270
Tru9I TTAA 2 cut(s) 30, 270
TseI GCWGC 1 cut(s) 195
TspDTI ATGAA 1 cut(s) 103
TspGWI ACGGA 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 204, 225
XspI CTAG 2 cut(s) 135, 245
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.